Multifunctional biomolecule nanostructures for cancer therapy

Nature Reviews Materials, Journal Year: 2021, Volume and Issue: 6(9), P. 766 - 783

Published: May 19, 2021

Language: Английский

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Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(5), P. 244 - 244

Published: April 26, 2023

Silver nanoparticles (AgNPs) are the most investigated antibacterial agents against multidrug resistant (MDR) pathogens. They can lead to cellular death by means of different mechanisms, damaging several cell compartments, from external membrane, enzymes, DNA and proteins; this simultaneous attack amplifies toxic effect on bacteria with respect traditional antibiotics. The effectiveness AgNPs MDR is strongly correlated their chemical morphological properties, which influence pathways involved in damage. In review, AgNPs’ size, shape modification functional groups or other materials reported, both investigate synthetic nanoparticles’ modifications evaluate related activity. Indeed, understanding conditions for obtaining performing could help tailor new improved silver-based combat resistance.

Language: Английский

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Challenges and Perspectives of DNA Nanostructures in Biomedicine

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(37), P. 15818 - 15833

Published: Feb. 29, 2020

DNA nanotechnology holds substantial promise for future biomedical engineering and the development of novel therapies diagnostic assays. The subnanometer-level addressability nanostructures allows their precise tailored modification with numerous chemical biological entities, which makes them fit to serve as accurate tools multifunctional carriers targeted drug delivery. absolute control over shape, size, function enables fabrication dynamic devices, such nanorobots that can execute programmed tasks react various external stimuli. Even though several studies have demonstrated successful operation both in vitro vivo, major obstacles remain on path real-world applications DNA-based nanomedicine. Here, we summarize current status field main implementations nanostructures. In particular, focus open challenges untackled issues discuss possible solutions.

Language: Английский

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Structure‐Based Varieties of Polymeric Nanocarriers and Influences of Their Physicochemical Properties on Drug Delivery Profiles

Advanced Science, Journal Year: 2022, Volume and Issue: 9(10)

Published: Feb. 3, 2022

Carriers are equally important as drugs. They can substantially improve bioavailability of cargos and safeguard healthy cells from toxic effects certain therapeutics. Recently, polymeric nanocarriers (PNCs) have achieved significant success in delivering drugs not only to but also subcellular organelles. Variety natural sources, availability different synthetic routes, versatile molecular architectures, exploitable physicochemical properties, biocompatibility, biodegradability presented polymers one the most desired materials for nanocarrier design. Recent innovative concepts advances PNC-associated nanotechnology providing unprecedented opportunities engineer their functions. The efficiency therapeutic loading has got considerably increased. Structural design-based varieties PNCs widely employed delivery small molecules genes, proteins. gained ever-increasing attention certainly paves way develop advanced nanomedicines. This article presents a comprehensive investigation structural influences properties on drug profiles with perspectives highlighting inevitability incorporating both multi-stimuli-responsive multi-drug single carrier design intelligent new emerging research directions this rapidly developing area.

Language: Английский

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Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(7), P. 3976 - 4050

Published: March 29, 2023

DNA nanotechnology is a unique field, where physics, chemistry, biology, mathematics, engineering, and materials science can elegantly converge. Since the original proposal of Nadrian Seeman, significant advances have been achieved in past four decades. During this glory time, origami technique developed by Paul Rothemund further pushed field forward with vigorous momentum, fostering plethora concepts, models, methodologies, applications that were not thought before. This review focuses on recent progress origami-engineered nanomaterials five years, outlining exciting achievements as well unexplored research avenues. We believe spirit assets Seeman left for scientists will continue to bring interdisciplinary innovations useful next decade.

Language: Английский

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Unraveling the interaction between doxorubicin and DNA origami nanostructures for customizable chemotherapeutic drug release

Nucleic Acids Research, Journal Year: 2021, Volume and Issue: 49(6), P. 3048 - 3062

Published: Feb. 4, 2021

Abstract Doxorubicin (DOX) is a common drug in cancer chemotherapy, and its high DNA-binding affinity can be harnessed preparing DOX-loaded DNA nanostructures for targeted delivery therapeutics. Although DOX has been widely studied, the existing literature of DNA-carriers remains limited incoherent. Here, based on an in-depth spectroscopic analysis, we characterize optimize loading into different 2D 3D scaffolded origami (DONs). In our experimental conditions, all DONs show similar binding capacities (one molecule per two to three base pairs), equilibrium reached within seconds, remarkably faster than previously acknowledged. To release profiles, DON degradation from complexes upon DNase I digestion was studied. For employed DONs, relative doses (DOX molecules released unit time) may vary by orders magnitude depending superstructure. addition, identify aggregation mechanisms spectral changes linked pH, magnesium, concentration. These features have largely ignored experimenting with nanostructures, but are probably major sources incoherence results so far. Therefore, believe this work act as guide tailoring profiles developing better systems DNA-carriers.

Language: Английский

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DNA Nanostructures: Current Challenges and Opportunities for Cellular Delivery

ACS Nano, Journal Year: 2021, Volume and Issue: 15(3), P. 3631 - 3645

Published: Feb. 26, 2021

DNA nanotechnology has produced a wide range of self-assembled structures, offering unmatched possibilities in terms structural design. Because their programmable assembly and precise control size, shape, function, particles can be used for numerous biological applications, including imaging, sensing, drug delivery. While the biocompatibility, programmability, ease synthesis nucleic acids have rapidly made them attractive building blocks, many challenges remain to addressed before using conditions. Enzymatic hydrolysis, low cellular uptake, immune cell recognition degradation, unclear biodistribution profiles are yet solved. Rigorous methodologies needed study, understand, fate structures physiological In this review, we describe current faced by field as well recent successes, highlighting potential solve biology problems or develop smart delivery tools. We then propose an outlook drive translation constructs toward preclinical particularly believe that detailed understanding nanostructures within living organisms, achieved through thorough characterization, is next required step reach clinical maturity.

Language: Английский

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DNA nanostructure-based nucleic acid probes: construction and biological applications

Chemical Science, Journal Year: 2021, Volume and Issue: 12(22), P. 7602 - 7622

Published: Jan. 1, 2021

In recent years, DNA has been widely noted as a kind of material that can be used to construct building blocks for biosensing, in vivo imaging, drug development, and disease therapy because its advantages good biocompatibility programmable properties. However, traditional DNA-based sensing processes are mostly achieved by random diffusion free probes, which were restricted limited dynamics relatively low efficiency. Moreover, the application biosystems, single-stranded probes face challenges such being difficult internalize into cells easily decomposed cellular microenvironment. To overcome above limitations, nanostructure-based have attracted intense attention. This probe showed series compared conventional ones, including increased biostability, enhanced cell internalization efficiency, accelerated reaction rate, amplified signal output, thus improved vitro applications. Therefore, reviewing summarizing important roles nanostructures improving biosensor design is very necessary development nanotechnology applications biology pharmacology. this perspective, reviewed summarized from several aspects: classification according dimensions (one, two, three-dimensional nanostructures), common connection modes between nucleic acid nanostructures, most self-assembled imaging analysis, assembly, capture, theranostics. Finally, prospects future also discussed.

Language: Английский

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A Self‐Catabolic Multifunctional DNAzyme Nanosponge for Programmable Drug Delivery and Efficient Gene Silencing

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(19), P. 10766 - 10774

Published: Feb. 18, 2021

Abstract DNAzyme‐based gene therapy holds immense prospects for effectively treating severe diseases, yet is constrained with inefficient delivery and unconditional activation. Herein, we designed a bioinspired self‐catabolic DNA nanocapsule sustaining tumor‐specific cascade activation of therapeutic DNAzyme. The exquisite DNAzyme was temporarily masked by the self‐excising in hierarchical rolling circle replication (RCR) nanostructures, thus stayed an inactive state physiological fluids. Through multivalent tumor‐anchoring aptamer strands, RCR specifically accumulated cancer cells sequentially activated motivating ultimate DNAzyme‐mediated silencing via intelligent stimuli‐responsive By virtue programmable assembly strategy, our compact nanoplatform shows great promise developing versatile smart therapeutics personalized nanomedicines.

Language: Английский

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Emerging Delivery Systems for Enabling Precision Nucleic Acid Therapeutics

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Nucleic acid therapeutics represent a highly promising treatment approach in modern medicine, treating diseases at the genetic level. However, these face numerous challenges practical applications, particularly regarding their stability, effectiveness, cellular uptake efficiency, and limitations delivering them specifically to target tissues. To overcome obstacles, researchers have developed various innovative delivery systems, including viral vectors, lipid nanoparticles, polymer inorganic protein carriers, exosomes, antibody oligonucleotide conjugates, DNA nanostructure-based systems. These systems enhance therapeutic efficacy of nucleic drugs by improving targeting specificity, half-life vivo. In this review, we systematically discuss different types drugs, analyze major barriers encountered delivery, summarize current research progress emerging We also highlight latest advancements application for diseases, infectious cancer, brain wound healing. This review aims provide comprehensive overview drug systems' status future directions integrating nanotechnology, biomaterials science, gene editing technologies, emphasizing transformative potential precision medicine.

Language: Английский

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