Activity-based NIR fluorescent probes based on the versatile hemicyanine scaffold: design strategy, biomedical applications, and outlook

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(5), P. 1795 - 1835

Published: Jan. 1, 2022

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.

Language: Английский

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Activity‐Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image‐Guided Surgery

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(32), P. 17268 - 17289

Published: Sept. 17, 2020

Abstract Near‐infrared (NIR) activatable fluorescent probes have been considered to be the effective edge tools for investigation of cell biology and disease diagnosis because their outstanding advantages. Related genes involved in tumor genesis progression regulate overexpression certain enzymes. Owing distinctive characteristics quick reaction time favorable pharmacokinetics, enzyme‐reactive NIR optical shown great potential tumorigenesis image‐guided intraoperative surgeries with high signal‐to‐noise ratios. In this review, we mainly summarize latest advancements from design strategy biomedical application. Specifically, some challenges prospects field are presented at end which will beneficial innovatively construct new multifunctional actively promote clinical transformation future.

Language: Английский

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Small-molecule fluorescence-based probes for interrogating major organ diseases

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(17), P. 9391 - 9429

Published: Jan. 1, 2021

This tutorial review describes recent advances involving small molecule fluorescent probes designed to aid in the study of major organ diseases.

Language: Английский

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Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(13), P. 6381 - 6389

Published: March 13, 2020

The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it difficult for surgeons to detect and accurately remove metastatic predominantly using visual examination palpation owing the lack effective means specifically distinguish boundary range between normal tumor tissues. Thus, development activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios particularly urgent in clinics. In view CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating progression, invasion, migration tumor, herein, we reported an APN-responsive YH-APN demonstrated its application cancer cells. Through situ spraying manner, (subcutaneous transplantation 13.86; hepatic metastasis, 4.42 6.25; splenic 4.99) were achieved. More importantly, have ability image metastasis less than 1 mm diameter, highlighting potential this be used tool This research may spur use enzyme-activatable probes progress diagnosis image-guided surgery (IGS).

Language: Английский

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Near-infrared (NIR) fluorescence-emitting small organic molecules for cancer imaging and therapy

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(21), P. 8957 - 9008

Published: Jan. 1, 2022

We discuss recent advances made in the development of NIR fluorescence-emitting small organic molecules for tumor imaging and therapy.

Language: Английский

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Fluorescent probes for the detection of disease-associated biomarkers

Science Bulletin, Journal Year: 2022, Volume and Issue: 67(8), P. 853 - 878

Published: Jan. 17, 2022

Language: Английский

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Activatable molecular probes for fluorescence-guided surgery, endoscopy and tissue biopsy

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 51(2), P. 566 - 593

Published: Dec. 20, 2021

The real-time, dynamic optical visualization of lesions and margins ensures not only complete resection the malignant tissues but also better preservation vital organs/tissues during surgical procedures. Most imaging probes with an "always-on" signal encounter high background noise due to their non-specific accumulation in normal tissues. By contrast, activatable molecular "turn on" signals upon reaction targeted biomolecules that are overexpressed cells, offering target-to-background ratios specificity sensitivity. This review summarizes recent progress diagnosis. design principle mechanism discussed, followed by specific emphasis on applications ranging from fluorescence-guided surgery endoscopy tissue biopsy. Finally, potential challenges perspectives field probe-enabled discussed.

Language: Английский

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Activity‐based smart AIEgens for detection, bioimaging, and therapeutics: Recent progress and outlook

Aggregate, Journal Year: 2021, Volume and Issue: 2(4)

Published: May 7, 2021

Abstract Activity‐based approaches for designing AIEgens possess prominent advantages including high selectivity, sensitivity, and signal‐to‐noise ratio, they have received more attention in recent years. Excellent activatable AIE probes been reported detecting toxic substances, imaging intracellular active molecules/biomolecules, as well monitoring the activity of overexpressed enzymes cancers. Moreover, majority theranostic can be specifically triggered cancer cells kill these under light irradiation, while no distinct effect on normal cells, demonstrating satisfactory therapeutic selectivity that is superior to traditional chemotherapy. Thus, this review, we systematically summarized development bioprobes years from molecular design principles biological applications. The challenges corresponding solutions are described. We hope information provided review will facilitate promote practical application AIEgens.

Language: Английский

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Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(16), P. 5607 - 5651

Published: Jan. 1, 2023

Since their inception, rhodamine dyes have been extensively applied in biotechnology as fluorescent markers or for the detection of biomolecules owing to good optical physical properties. Accordingly, they emerged a powerful tool visualization living systems. In addition fluorescence bioimaging, molecular design derivatives with disease therapeutic functions (e.g., cancer and bacterial infection) has recently attracted increased research attention, which is significantly important construction libraries diagnostic integration. However, reviews focusing on integrated strategies dye-based diagnosis treatment wide application are extremely rare. this review, first, brief history development dyes, transformation from bioimaging therapy, concept optics-based integration its significance human presented. Next, systematic review several excellent rhodamine-based well treatment, Finally, challenges practical future outlook clinical translation also discussed.

Language: Английский

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Organic dye assemblies with aggregation‐induced photophysical changes and their bio‐applications

Aggregate, Journal Year: 2021, Volume and Issue: 2(4)

Published: Feb. 23, 2021

Abstract Phototheranostics provide a safe, effective, and noninvasive way for the diagnosis treatment of contemporary diseases, organic dyes play vital role. For example, chemical modification endowed with powerful reactive oxygen species or heat generation ability, favoring photodynamic therapy photoacoustic (PA) imaging guided photothermal (PTT) serious diseases. Therefore, photophysical properties manipulation has become focus in current dye chemistry research. The development aggregate science made great effort to solve this problem. In recent years, large number studies have focused on molecular aggregation behavior its effect performance. most famous example is discovery aggregation‐induced emission (AIE) phenomenon. Based AIE theory, more theories revealing relationship between were proposed elucidated. property changes caused by unique discipline, guiding material science. With help self‐assembly, controllable can be realized, stable nano‐theranostic reagents obtained. Furthermore, constructing assemblies various will greatly reduce cost theranostic reagents, thus, expanding biomedical applications dyes. review focuses characteristic their biological including, fluorescence/phosphorescence/PA as well PTT. This guidance design dyes, methods, construction multifunctional phototheranostic reagents.

Language: Английский

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