ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
We
present
a
strategy
for
constructing
activatable
photoacoustic
imaging
(PAI)
probes
in
vivo
enzyme
activity
measurements,
based
on
dissociation
previously
applied
to
vitro
sensing.
Unlike
conventional
nanoparticle
aggregation
strategies,
minimizes
false
positives
and
functions
effectively
complex
biological
environments.
Overcoming
the
challenge
of
dissociating
nanostructure
aggregates,
which
arises
from
strong
van
der
Waals
forces
at
short
distances,
we
demonstrate
controlled
assembly
citrate-capped
gold
nanorods
(AuNRs-citrate)
using
diarginine
peptide
additive
thiolated
polyethylene
glycol
(HS-PEG-OMe),
respectively.
This
mechanism
enables
precise
control
optical
(PA)
properties
AuNRs
both
settings.
Building
these
findings,
engineered
an
enzyme-sensitive
PAI
probe
(AuNRs@RgpB)
composed
AuNR
assemblies
PEG-peptide
conjugate
with
protease-specific
cleavage
sequence.
The
detects
Arg-specific
gingipain
(RgpB),
protease
expressed
by
Porphyromonas
gingivalis
associated
periodontal
disease
Alzheimer's
disease.
Proteolytic
sequence
triggers
dissociation,
resulting
enhanced
PA
signal
output.
was
designed
be
injected
intrathecally
preclinical
trials
image
gingipains
investigate
value
inhibitors
developed
probe's
performance
characterized
UV-vis
spectroscopy
PAI,
achieving
detection
limits
5
20
nM,
In
studies
involved
intracranial
injection
AuNRs@RgpB
into
RgpB-containing
murine
models,
monitoring
over
time.
RgpB
produced
four-fold
increase
within
2
h,
while
P.
gingivalis-infected
mice
showed
similar
enhancement.
Specificity
confirmed
negligible
responses
Fusobacterium
nucleatum,
non-RgpB-producing
bacterium.
Additionally,
system
demonstrated
utility
drug
development
successfully
inhibition
(leupeptin
KYT-1)
models.
Beyond
its
immediate
application
detection,
this
modular
approach
plasmonic-based
sensing
holds
significant
potential
detecting
other
proteases,
advancing
nanotechnology
protease-targeted
diagnostics.
Chemical Society Reviews,
Journal Year:
2024,
Volume and Issue:
53(6), P. 2932 - 2971
Published: Jan. 1, 2024
Silver
and
gold
nanoparticles
have
found
extensive
biomedical
applications
due
to
their
strong
localized
surface
plasmon
resonance
(LSPR)
intriguing
plasmonic
properties.
This
review
article
focuses
on
the
correlation
among
particle
geometry,
properties
applications.
It
discusses
how
shape
size
are
tailored
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(12), P. 8649 - 8662
Published: March 12, 2024
There
has
been
much
interest
in
integrating
various
inorganic
nanoparticles
(nanoscale
colloids)
biology
and
medicine.
However,
buildup
of
a
protein
corona
around
the
biological
media,
driven
by
nonspecific
interactions,
remains
major
hurdle
for
translation
nanomedicine
into
clinical
applications.
In
this
study,
we
investigate
interactions
between
gold
serum
proteins
using
series
dihydrolipoic
acid
(DHLA)-based
ligands.
We
employed
gel
electrophoresis
combined
with
UV–vis
absorption
dynamic
light
scattering
to
correlate
adsorption
nature
size
ligand
used.
For
instance,
found
that
AuNPs
capped
DHLA
alone
promote
adsorption.
comparison,
capping
polyethylene
glycol-
or
zwitterion-appended
essentially
prevents
formation,
regardless
charge
size.
Our
results
highlight
crucial
role
surface
chemistry
core
material
formation
offer
valuable
information
design
colloidal
nanomaterials
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(7), P. 689 - 689
Published: June 28, 2023
The
utilization
of
electrochemical
detection
techniques
in
paper-based
analytical
devices
(PADs)
has
revolutionized
point-of-care
(POC)
testing,
enabling
the
precise
and
discerning
measurement
a
diverse
array
(bio)chemical
analytes.
application
sensing
paper
as
suitable
substrate
for
testing
platforms
led
to
emergence
(ePADs).
inherent
advantages
these
modified
have
gained
significant
recognition
POC
field.
In
response,
biosensors
assembled
from
materials
shown
great
promise
enhancing
sensitivity
improving
their
range
use.
addition,
numerous
advantageous
characteristics,
including
self-sufficient
conveyance
liquids,
reduced
resistance,
minimal
fabrication
cost,
environmental
friendliness.
This
study
seeks
provide
concise
summary
present
state
uses
ePADs
with
insightful
commentary
on
practicality
Future
developments
include
developing
novel
systems,
system
performance
biocatalyst,
combining
biosensor
other
cutting-edge
tools
such
machine
learning
3D
printing.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(5), P. 2713 - 2722
Published: Jan. 27, 2023
We
detail
the
assembly
and
characterization
of
quantum
dot
(QD)−dye
conjugates
constructed
using
a
peptide
bridge
specifically
designed
to
recognize
interact
with
breast
cancer
biomarker─matrix
metalloproteinase-14
(MMP-14).
The
assembled
QD
are
then
used
as
optically
addressable
probes,
relying
on
Förster
resonance
energy
transfer
(FRET)
interactions
transduction
mechanism
detect
activity
MMP-14
in
solution
phase.
QDs
were
first
coated
dithiolane
poly(ethylene
glycol)
(PEG)
bearing
carboxyl
group
that
allows
coupling
via
amide
bond
formation
different
dye-labeled
peptides.
analytical
capability
is
enabled
by
correlating
changes
FRET
efficiency
conjugate
valence
and/or
QD-to-dye
separation
distance,
triggered
modulated
enzymatic
proteolysis
surface-tethered
probe
exhibits
great
sensitivity
enzyme
digestion
sub-nanomolar
limit
detection.
further
analyze
data
within
framework
Michaelis–Menten
model,
which
considers
fact
surface-attached
peptides
have
slower
diffusion
coefficient
than
free
This
results
reduced
collision
frequency
lower
catalytic
efficiency,
kcat/KM.
Our
suggest
our
design
promising,
effective,
potentially
useful
for
vivo
analysis.
Nano Convergence,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Jan. 8, 2024
Abstract
The
COVID-19
outbreak
caused
by
SARS-CoV-2
in
late
2019
has
spread
rapidly
across
the
world
to
form
a
global
epidemic
of
respiratory
infectious
diseases.
Increased
investigations
on
diagnostic
tools
are
currently
implemented
assist
rapid
identification
virus
because
mass
and
diagnosis
might
be
best
way
prevent
virus.
This
critical
review
discusses
detection
principles,
fabrication
techniques,
applications
with
three
categories:
nuclear
acid
augmentation
test,
immunoassay
test
biosensors.
Special
efforts
were
put
enhancement
nanomaterials
biosensors
for
rapid,
sensitive,
low-cost
diagnostics
Future
developments
suggested
regarding
potential
candidates
hospitals,
clinics
laboratories
control
prevention
large-scale
epidemic.
Graphical
ACS Nano,
Journal Year:
2022,
Volume and Issue:
16(4), P. 6165 - 6175
Published: April 4, 2022
We
report
the
peptide-programmed
fractal
assembly
of
silver
nanoparticles
(AgNPs)
in
a
diffusion-limited
aggregation
(DLA)
mode,
and
this
change
morphology
generates
significant
color
change.
show
that
peptides
with
specific
repetitions
defined
amino
acids
(i.e.,
arginine,
histidine,
or
phenylalanine)
can
induce
coalescence
AgNPs
(20
nm)
into
hyperbranched
structure
(AgFSs)
(∼2
μm).
The
dynamic
process
was
systematically
investigated,
extinction
nanostructures
be
modulated
from
400
to
600
nm
by
varying
peptide
sequences
molar
ratio.
According
rationale,
two
strategies
SARS-CoV-2
detection
were
investigated.
activity
main
protease
(Mpro)
involved
validated
substrate
bridge
after
proteolytic
cleavage.
A
sub-nanomolar
limit
(0.5
nM)
capacity
distinguish
naked
eye
wide
concentration
range
(1.25–30
achieved.
Next,
multichannel
sensor-array
based
on
multiplex
visually
proteases
influenza
doped
human
samples
