Cracking the membrane lipid code

Current Opinion in Cell Biology, Journal Year: 2023, Volume and Issue: 83, P. 102203 - 102203

Published: July 11, 2023

Why has nature acquired such a huge lipid repertoire? Although it would be theoretically possible to make bilayer fulfilling barrier functions with only one glycerophospholipid, there are diverse and numerous different species. Lipids heterogeneously distributed across the evolutionary tree lipidomes evolving in parallel organismal complexity. Moreover, lipids between organs tissues even within same cell, organelles have characteristic signatures. At molecular level, membranes asymmetric laterally heterogeneous. This asymmetry at scales indicates that these molecules may play very specific biology. Some of roles been recently uncovered: shown essential processes as hypoxia ferroptosis or protein sorting trafficking but many them remain still unknown. In this review we will discuss importance understanding diversity biology share toolbox some emerging technologies helping us uncover new cell and, step by step, crack membrane code.

Language: Английский

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Hydrophobic Interfacing of Fluorescent Membrane Probes

ChemistryEurope, Journal Year: 2023, Volume and Issue: 1(3)

Published: Oct. 2, 2023

Abstract Fluorescent flippers have been introduced as small‐molecule probes to image membrane tension in living systems. While the hydrophilic headgroup region has modified extensively for intracellular targeting, little is known about hydrophobic interfacing with surrounding membrane. To tackle this challenge, design, synthesis and evaluation of a glutamine‐derived flipper collection reported. Considering importance tension‐induced phase separation imaging, study focused on how modulate distribution functional between ordered disordered microdomains. Also interest was control over intermembrane transfer without loss function specific labeling plasma membranes. Evidence presented two‐step insertion mechanism through more accessible domains into better matching domains. This process also explains differences partition coefficients bioimaging. It further demonstrated that interdomain can be regulated by brightness fluorescence lifetime imaging microscopy responsiveness tension. Irreversible partitioning inhibits coincides internalization cells. These results demonstrate improve probe performance provide guidelines proceed.

Language: Английский

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Pentafluorobenzene: Promising Applications in Diagnostics and Therapeutics

ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(10), P. 4081 - 4099

Published: Sept. 18, 2023

Pentafluorobenzene (PFB) represents a class of aromatic fluorine compounds employed exclusively across spectrum chemical and biological applications. PFBs are credited with developing various synthesis techniques, networks biopolymers, bioactive materials, targeted drug delivery systems. The first part this review delves into recent developments in PFB-derived molecules for diagnostic purposes. In the latter segment, PFB's role domain theragnostic applications is discussed. elucidates different mechanisms interaction strategies applied leveraging to formulate tools, substantiated by proper examples. utilization emerges as an enabler, facilitating manifold reactions, improving materials' properties, even opening avenues explorative research.

Language: Английский

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Spectrally Resolved Single Molecule Orientation Imaging Reveals Direct Correspondence between Polarity and Order Experienced by Nile Red in Supported Lipid Bilayer Membrane

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

ABSTRACT Molecular level interaction among lipids, cholesterol and water dictates nanoscale membrane organization of lipid bilayers into liquid ordered (Lo) disordered (Ld) phases, characterized by different polarity order. Generally, solvatochromic dyes easily discriminate difference between Lo Ld whereas molecular flippers rotors show distinct photophysics depending on In spite progress in single molecule spectral imaging orientation mapping, still direct experimental proof linking with order sensed the same probe eludes us. Here, we demonstrate spectrally resolved localization microscopy to connect nanoscopic bilayer its emission spectra, three-dimensional dipole rotational constraint offered local microenvironment highlights beautiful correspondence This technique has potential addres heterogeneity dynamics, especially biology as well material sciences. TOC GRAPHICS

Language: Английский

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Single-Molecule Localization Microscopy and Tracking with a Fluorescent Mechanosensitive Probe

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: 128(33), P. 7997 - 8006

Published: Aug. 9, 2024

A milestone in optical imaging of mechanical forces cells has been the development family flipper fluorescent probes able to report membrane tension noninvasively living through their fluorescence lifetime. The specifically designed Flipper-CF3 probe with an engineered inherent blinking mechanism was recently introduced for super-resolution microscopy lipid ordered membranes but too dim be detected disordered at single-molecule level (García-Calvo, J. Am. Chem. Soc. 2020, 142(28), 12034–12038). We show here that original and commercially available Flipper-TR is compatible based resolves both liquid giant unilamellar vesicles below diffraction limit. Single molecules were additionally tracked bilayers, enabling distinguish varying composition from diffusion coefficient probe. Differences brightness between originate steady-state absorption properties. general compatibility scaffold detection further shown experiments targetable derivatives.

Language: Английский

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