Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
The designed all-in-one SNAs anchored with ice-cream probes achieve three functions: (i) enhanced AMACR conversion efficiency; (ii) promoted rolling circle amplification and (iii) improved CRISPR/Cas12a activation efficiency.
Language: Английский
Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(44)
Published: Sept. 12, 2023
Copper nanoclusters (CuNCs) are emerging electrochemiluminescence (ECL) emitters with unique molecule-like electronic structures, high abundance, and low cost. However, the synthesis of CuNCs ECL efficiency stability in a scalable manner remains challenging. Here, we report facile gram-scale approach for preparing self-assembled (CuNCsAssy ) induced by ligands exceptionally boosted anodic stability. Compared to disordered aggregates that inactive ECL, CuNCsAssy shows record (10 %, wavelength-corrected, relative Ru(bpy)3 Cl2 /tripropylamine). Mechanism studies revealed unusual dual functions simultaneously facilitating electrochemical excitation radiative transition. Moreover, assembly addressed limitation poor conventional CuNCs. As proof concept, an biosensor alkaline phosphatase detection was successfully constructed ultralow limit 8.1×10-6 U/L.
Language: Английский
Citations
46
ACS electrochemistry., Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 15, 2025
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(17), P. 6652 - 6658
Published: April 17, 2024
A low-triggering potential and a narrow-potential window are anticipated to decrease the electrochemical interference cross talk of electrochemiluminescence (ECL). Herein, by exploiting low oxidative (0.82 V vs Ag/AgCl) dihydrolipoic acid-capped sliver nanoclusters (DHLA-AgNCs), coreactant ECL system DHLA-AgNCs/hydrazine (N2H4) is proposed achieve efficient oxidative-reduction with 0.82 (vs 0.22 V. The low-triggering-potential narrow-potential-window nature can be primarily preserved upon labeling DHLA-AgNCs probe DNA immobilizing onto Au surface via sandwiched hybridization, which eventually enables selective strategy for gene assay at +0.82 This sensitively determine human papillomavirus from 10 1000 pM limit detection 5 (S/N = 3) would open way improve applied bioassay.
Language: Английский
Citations
9
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(4), P. 1700 - 1706
Published: Jan. 18, 2024
The commercialized electrochemiluminescence (ECL) immunoassay is carried out by holding luminophore Ru(bpy)32+ at a given potential. Designing an electrochemiluminophore with narrow triggering potential window strongly anticipated to decrease the electrochemical cross-talk and improve flux of ECL in potential-resolved way. Herein, L-penicillamine-capped silver nanoclusters (LPA-AgNCs) are facilely synthesized utilized as tags perform sole 0.24 V employing hydrazine (N2H4) coreactant. maximum emission LPA-AgNCs/N2H4 system located ca. +1.27 V. Upon immobilizing LPA-AgNCs onto electrode surface via forming sandwich immunocomplex, can be sensitively selectively determine human carcinoembryonic antigen from 0.5 1000 pg/mL low limit detection 0.1 (S/N = 3). This work might open way screen electrochemiluminophores for multiple
Language: Английский
Citations
8
Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 153, P. 104706 - 104706
Published: Sept. 10, 2024
Language: Английский
Citations
8
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4623 - 4631
Published: March 8, 2024
Developing new electrochemiluminescence (ECL) luminators with high stability, wide applicability, and strong designability is of great strategic significance to promote the ECL field frontier. Here, driven by I···N bond, 1,3,5-trifluoro-2,4,6-triiodobenzene (TFTI) 2,4,6-trimethyl-1,3,5-triazine (TMT) self-assembled into a novel halogen cocrystal (TFTI-TMT) through slow solution volatilization. Significant difference charge density existed between N atoms on TMT σ-hole I TFTI. Upon induction effect, high-speed spontaneous transferring from TFTI occurred, stimulating exciting signals. Besides, could capture iodine ions specifically, which blocked original transfer σ-hole, causing signal TFTI-TMT undergo quenching rate as 92.9%. Excitingly, sensing toward I– possessed linear range (10–5000 nM) ultralow detection limit (3 in real water sample. The strategy makes remarkable viewpoint luminator design enables analysis technology contribute addressing environmental health threats posed iodide pollution.
Language: Английский
Citations
7
Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 21, 2025
In this study, ionic-liquid submicron particles (ILSPs) encapsulating the luminophore tris(2',2-bipyridyl)ruthenium (II) ([Ru(bpy)3]2+) were developed as a carrier for an electrochemiluminescence immunoassay (ECLIA). The ILSPs applied to quantitative determination of model analyte prostate-specific antigen (PSA). [Ru(bpy)3]2+ was measured with 2-(dibutylamino)ethanol co-reactant in nine ionic liquids (ILs). intensity higher 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([BMIM][TFSA]) and N-(2-methoxyethyl)-N-methyl pyrrolidinium ([MEMP][TFSA]) than other ILs. prepared using polyoxyethylene sorbitan monolaurate monooleate surfactants either [BMIM][TFSA] or [MEMP][TFSA]. had mean particle size 244 nm zeta potential - 21.0 mV, [MEMP][TFSA] 293 17.9 mV. Microscope images showed that IL-in-water emulsions completely encapsulated [Ru(bpy)3]2+. more stable those [MEMP][TFSA], selected ECLIA PSA determination. calibration curve good linear relationship (y = 0.29x + 4.02, r 0.95) concentration range 100 pg/mL-100 μg/mL. limit detection quantification 544 pg/mL 35 ng/mL, respectively. Our results demonstrate can be used easily determine even state.
Language: Английский
Citations
1
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115530 - 115530
Published: July 16, 2023
Language: Английский
Citations
17
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 170, P. 117443 - 117443
Published: Nov. 18, 2023
Language: Английский
Citations
17
JACS Au, Journal Year: 2023, Volume and Issue: 3(9), P. 2566 - 2577
Published: Sept. 6, 2023
Metal nanoclusters (NCs) have emerged as a promising class of fluorescent probes for cellular imaging due to their high resistance photobleaching and low toxicity. Nevertheless, widespread use in clinical diagnosis is limited by unstable intracellular fluorescence. In this study, we develop an intracellularly biosynthesized probe, DNA nanoribbon-gold NCs (DNR/AuNCs), long-term tracking. Our results show that DNR/AuNCs exhibit 4-fold enhancement fluorescence intensity compared free AuNCs. We also investigated the mechanism underlying AuNCs DNRs. findings suggest higher synthesis efficiency stability lysosome may contribute enhancement, which enables (up 15 days) cancer cells (enhancement ∼60 times AuNCs). Furthermore, observe similar with other metal NCs, confirming generality DNR-assisted biosynthesis approach preparing highly bright stable cell imaging.
Language: Английский
Citations
16