‘Roly-poly toy’ motion during pollen exudation promotes rapid pollen adhesion in rice

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 18, 2025

Abstract In angiosperm, successful pollen adhesion and hydration on the stigma are essential for germination tube elongation. Self-pollinated grass plants, such as rice, exhibit viscous ‘pollen exudation’ prior to adhesion; however, its cellular dynamics, including their chemical composition, remain unknown. Here, we revisit exudation in rice find that grains showed ‘Roly-poly toy’-like rocking motion exudates lead adhesion. Single-cell metabolomics revealed were composed of high content sugar together with fatty acids redox-related metabolites, different from mature cells. And hence, these solutes might participate osmotic molecular signaling stigmatic apoplast, increasing fluid viscosity. Taken together, it is concluded unique behaviour observed play a crucial role optimal self-positioning germination, resulting rapid self-pollination.

Language: Английский

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Electroless Ionization Mass Spectrometry Using a Compact Electrokinetic Ionization Source

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(27), P. 10978 - 10985

Published: June 25, 2024

We introduce a new ionization technique for compact, portable mass spectrometers. It consists of syringe with sample liquid capped by self-ionizing spray nozzle containing microfabricated chip. Interaction the wall results in electrical charging without need electronics. Elaborate cleaning procedures are redundant when disposable syringes and mass-fabricated nozzles used. This self-named electroless (ELI) shows comparable performance to conventional techniques. In contrast commonly used electrospray ionization, ELI exhibits excellent efficiency low-conductive solutions such as water or acetonitrile. Due its compact size absence high-voltage electronics, it can also be readily integrated other sources. Besides reviewing main properties ELI, we showcase technique's potential two on-site, ambient spectroscopy applications: perfume fingerprinting fast screening fungicides on citrus fruits.

Language: Английский

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Identification of MDA in seized ecstasy-like samples using atmospheric solids analysis probe mass spectrometry and machine learning

Microchemical Journal, Journal Year: 2024, Volume and Issue: 205, P. 111287 - 111287

Published: July 24, 2024

Language: Английский

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In-Line Thermal Desorption and Dielectric Barrier Discharge Ionization for Rapid Mass Spectrometry Detection of Explosives

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(33), P. 13352 - 13357

Published: Aug. 5, 2024

Thermal desorption (TD) of wipe-based samples was coupled with an in-line dielectric barrier discharge ionization (DBDI) source and rugged compact time-of-flight mass spectrometer (MS) for the detection explosives, propellants, postblast debris. The chromatography-free TD-DBDI-MS platform enabled rapid sensitive organic nitramine, nitrate ester, nitroaromatic explosives as well black powder substitute propellants. Parametric investigations characterized response to TD temperature optimized DBDI voltage, aerodynamically assisted entrainment, fragmentation through in-source collision induced dissociation (isCID). Excess generated by yielded predominantly nitrate-adduct formation. Subnanogram sensitivities were demonstrated all investigated, except nitroglycerin, specifically due its volatility. Further, most analytes/explosives exhibited tens picograms sensitivities. also propellant military from system performed without need entrainment (and associated rough pump), which along requiring no additional gases (i.e., N

Language: Английский

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Current chromatographic methods to determine cannabinoids in biological samples: a review of the state-of-the art on sample preparation techniques

Green Analytical Chemistry, Journal Year: 2024, Volume and Issue: 11, P. 100161 - 100161

Published: Oct. 6, 2024

Language: Английский

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Sample Injection for Real-Time Analysis (SIRTA) Using GC-MS with Cold EI

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: 35(2), P. 378 - 385

Published: Jan. 18, 2024

There is a continual demand for advanced methods and instruments real-time analysis (RTA). Most of the current RTA techniques based on MS involve ambient desorption ionization technology. However, flow injection liquid extracted samples another option without added modifications or cost to existing LC-MS instruments. In this work, we introduce new approach named sample (SIRTA) using GC-MS with Cold EI. SIRTA, standard GC column replaced 1 m long 0.1 mm I.D. fused silica capillary that connects injector transfer-line Thus, SIRTA EI imposes no need any additional instrumentation; hence, it characterized by zero cost. Like in LC-MS, dissolved ∼1 mL methanol solvent. Subsequently, vial placed autosampler while syringe separation. The takes merely 0.2-0.7 min, ensuring rapid consecutive analyses. Unlike EI, enables taking advantage its fly through open ion source avoid overwhelming during elution solvents still providing enhanced molecular ions nearly all analytes. study, demonstrated 12 compounds 7 mixtures, including various prescription illicit drugs, cannabis petroleum samples, other synthetic organic those weight up 800 g/mol.

Language: Английский

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Arc-Induced Electrospray Ionization Mass Spectrometry

Analytical Chemistry, Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 28, 2023

Arc-induced electrospray ionization mass spectrometry (AESI-MS) was developed during which alternating current is simply achieved through the arc plasma. The AESI source exploits arc's temperature and charge properties to generate aerosols consisting of charged microdroplets. region, in organic molecules are contained within microdroplets, partially overlaps with plasma region. Guided by electric field, these undergo ionization, yielding ionic target analytes. represents a soft method that combines mechanisms atmospheric pressure chemical facilitating analytes wide ranging polarities. precisely targeted spraying area enhances ion entry into analyzer, thereby enabling excellent efficiency. exhibits several notable advantages over source, including an elevated but comparable level active species concentrations types, simplified spectra for direct amino acid analysis, high salt tolerance, versatile analysis compounds varying polarities, reliable quantitative acids complex matrices. Overall, broadens methodologies employed providing technological scientific framework creating distinctive techniques.

Language: Английский

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Maize hydroxycinnamic acids: unveiling their role in stress resilience and human health

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Feb. 2, 2024

Maize production is pivotal in ensuring food security, particularly developing countries. However, the crop encounters multiple challenges stemming from climatic changes that adversely affect its yield, including biotic and abiotic stresses during storage. A promising strategy for enhancing maize resilience to these involves modulating hydroxycinnamic acid amides (HCAAs) content. HCAAs are secondary metabolites present plants essential developmental processes, substantially contributing defense mechanisms against environmental stressors, pests, pathogens, exhibiting beneficial effects on human health. This mini-review aims provide a comprehensive overview of maize, their biosynthesis, functions, distribution, health potential applications.

Language: Английский

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Developing a detection strategy for ten paralytic shellfish poisonings in urine, combining high-throughput DESI-MS screening and accurate UPLC-QqQ/MS quantification

Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1234, P. 124036 - 124036

Published: Feb. 1, 2024

Language: Английский

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Protein analysis by desorption electrospray ionization mass spectrometry

Mass Spectrometry Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: July 26, 2024

Abstract This review presents progress made in the ambient analysis of proteins, particular by desorption electrospray ionization‐mass spectrometry (DESI‐MS). Related ionization techniques are discussed comparison to DESI‐MS only illustrate larger context protein mass spectrometry. The describes early and current approaches for undigested native tryptic digests, indirect determination through reporter molecules. Applications imaging spatial distributions, identification posttranslational modifications, binding stoichiometries, enzymatic transformations discussed. analytical capabilities other such as LESA nano‐DESI currently exceed those situ surface sampling intact proteins from tissues. shows, however, that despite its many limitations, is making valuable contributions analysis. challenges sensitivity, resolution, range surmountable obstacles further development improvements justified.

Language: Английский

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