Cited by De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis

Metabolic engineering of Escherichia coli BL21(DE3) cocultured with glucose and xylose for efficient production of 2′-fucosyllactose DOI

Yunqi Zhu,

Mingli Zhao,

Hao Wang

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132062 - 132062

Published: Jan. 1, 2025

Language: Английский

Citations

Metabolic engineering of Priestia megaterium for 2’-fucosyllactose production DOI

Bu-Soo Park,

Jihee Yoon,

Jun-Min Lee

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 4, 2025

Abstract Background 2′-Fucosyllactose (2′-FL) is a predominant human milk oligosaccharide that significantly enhances infant nutrition and immune health. This study addresses the need for safe economical production of 2’-FL by employing Generally Recognized As Safe (GRAS) microbial strain, Priestia megaterium ATCC 14581. strain was chosen its robust growth established safety profile attributing suitable industrial-scale production. Results The engineering targets included deletion lacZ gene to prevent lactose metabolism interference, introduction α-1,2-fucosyltransferase derived from non-pathogenic optimization GDP-L-fucose biosynthesis pathway through overexpression manA manC . These changes, coupled with improvements in uptake utilization random mutagenesis, led high yield 28.6 g/L fed-batch fermentation, highlighting potential our metabolic strategies on P. Conclusions GRAS 14581 successfully engineered overproduce 2’-FL, valuable oligosaccharide, series genetic modifications optimizations. work underscores feasibility using strains oligosaccharides, paving way safer more efficient methods biotechnological applications. Future studies could explore additional fermentation conditions further enhance scalability.

Language: Английский

Citations

Metabolic Engineering of Escherichia coli BL21(DE3) for 2′-Fucosyllactose Synthesis in a Higher Productivity DOI

Na Li, Yan Song,

Hongzhi Xia

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

2′-Fucosyllactose (2′-FL) is the most abundant human milk oligosaccharides (HMOs). 2′-FL exhibits great benefits for infant health, such as preventing infantile diarrhea and promoting growth of intestinal probiotics. The microbial cell factory technique has shown promise massive production 2′-FL. Here, we aimed to construct a recombinant E. coli BL21(DE3) strain hyperproduction Initially, multicopy genomic integration expression lactose permease gene lacY reduced formation byproducts. Furthermore, more efficient Shine–Dalgarno sequence was used replace wild-type in manC-manB gmd-wcaG clusters, which significantly increased titer. Based on these results, overexpressed sugar efflux transporter SetA knocked out pgi gene. This further improved synthesis when glycerol sole carbon source. Finally, new α-1,2-fucosyltransferase identified Neisseria sp., exhibited higher capacity production. Fed-batch fermentation produced 141.27 g/L 45 h with productivity 3.14 × h. rate achieved highest recorded levels, indicating potential engineered BL21 (DE3) strains use industrial

Language: Английский

Citations

Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system DOI

Yi Li, Xiang Wang,

Kaidi Chen

et al.

Synthetic and Systems Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

2'-fucosyllactose (2'-FL) holds significant role in the infants' nutrition. While microbial production of 2'-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, potential Pichia pastoris, renowned for its robust NADPH regeneration capability, remains underexplored. Herein, we systematically engineered metabolism P. pastoris to develop an efficient 2'-FL-producing cell factory. We first constructed de novo biosynthesis pathway achieving initial titer 0.143 g/L. By optimizing enzyme selection solubility α-1,2-fucosyltransferase (FutC), was enhanced by nearly ten folds. Subsequently, engineering supply further increased 170 %. Furthermore, energy incorporating orthogonal module based on methanol dissimilation increasing GTP availability, resulting a 32 % improvement production. Finally, through optimization fermentation condition, realized 3.50 g/L shake-flask, representing highest pastoris. These findings highlight as chassis produce chemicals providing abundant utilizing co-substrate sufficient energy.

Language: Английский

Citations

Structure, Function, Synthesis and Improved Strategies of Fucosylated Human Milk Oligosaccharides and Their Future Perspectives: A Review DOI

Ya-Ya Yang,

Shunli Jing,

Le Zhang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106584 - 106584

Published: April 1, 2025

Language: Английский

Citations

Metabolic engineering Corynebacterium glutamicum ATCC13032 for 2′-fucosyllactose production DOI

Zihan Li,

Guihong Zhao,

Dezhi Zhang

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway DOI

Shanquan Liang,

Zi He,

Dan Liu

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(1), P. 108 - 114

Published: Jan. 6, 2024

2'-Fucosyllactose (2'-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis 2'-FL by

Language: Английский

Citations

De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis DOI

Quanwei Zhang, X. Xu, Mengli Zhang

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations