Targeting cholangiocarcinoma cells by cold piezoelectric plasmas: in vitro efficacy and cellular mechanisms DOI Creative Commons
Manon Soulier, Bouchra Lekbaby,

Imane Houari

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 4, 2024

Cold piezoelectric plasma (CPP) is a novel approach in cancer therapy, enabling the development of portable treatment devices capable triggering cell death. While its effectiveness remains underexplored, this research focuses on application against cholangiocarcinoma (CCA), an aggressive biliary tract. A CPP device utilized to generate either corona discharge (Pz-CD) or dielectric barrier (Pz-DBD) for vitro experiments. Notably, Pz-CD can deliver more power than Pz-DBD, although both sources produce significant levels reactive species and liquid phases. This work shows that causes gradient increase medium temperature from center towards edges culture well, especially longer times. Although heats significantly, it cools quickly after extinction. When applied human CCA cells, immediate long-term effects, localized Pz-CD, while uniform Pz-DBD. Immediate effects result also actin cytoskeleton remodeling without alteration membrane permeability. Long-term CPP, antioxidant system engaged, include activation DNA damage response pathway leading In conclusion, should be recognized as promising antitumor therapy.

Language: Английский

Cold Atmospheric Plasma as a Therapeutic Tool in Medicine and Dentistry DOI
Cristiane Yumi Koga‐Ito, Konstantin Georgiev Kostov, F. Miranda

et al.

Plasma Chemistry and Plasma Processing, Journal Year: 2023, Volume and Issue: 44(3), P. 1393 - 1429

Published: Aug. 27, 2023

Language: Английский

Citations

29
Cancer stem cells and their niche in cancer progression and therapy DOI Creative Commons

Qiuping Liu,

Zongliang Guo,

Guoyin Li

et al.

Cancer Cell International, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 1, 2023

Abstract High recurrence and metastasis rates poor prognoses are the major challenges of current cancer therapy. Mounting evidence suggests that stem cells (CSCs) play an important role in development, chemoradiotherapy resistance, recurrence, metastasis. Therefore, targeted CSC therapy has become a new strategy for solving problems recurrence. Since properties CSCs regulated by specific tumour microenvironment, so-called niche, which targets crosstalk between their niches, is vital our pursuit therapeutic opportunities to prevent from recurring. In this review, we aim highlight factors within niche have roles regulating properties, including extracellular matrix (ECM), stromal (e.g., associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), mesenchymal (MSCs)), physiological changes inflammation, hypoxia, angiogenesis). We also discuss recent progress regarding therapies targeting elucidate developments more effective strategies eliminate cancer.

Language: Английский

Citations

25
Cold atmospheric plasma (CAP): a revolutionary approach in dermatology and skincare DOI Creative Commons
Ahmad Taha Khalaf, Ahmed N. Abdalla,

Kaixuan Ren

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Oct. 5, 2024

Language: Английский

Citations

6
Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines DOI Creative Commons
Viviana di Giacomo, Marwa Balaha,

Morena Pinti

et al.

Oral Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Objective Cold atmospheric plasma (CAP) is a novel approach for cancer treatment. It can be used to treat liquids—plasma‐activated media (PAM)—which are then transferred the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM assessing their in vitro selectivity against head neck cells (HNC). Methods were obtained by exposing 2 5 mL cell culture medium CAP 5, 10 20 min 6 mm working distance. Anions kinetics was evaluated ion chromatography. Cell proliferation inhibition, apoptosis occurrence, cycle modifications assessed MTS flow cytometry, on human epidermal keratinocyte (HaCaT) HNC lines HSC3, HSC4 A253. Results conditions showed significant reduction whereas effect milder, but time‐dependence more evident. HaCaT unaffected PAM, indicating cells. Conclusions chemical composition modified exposure influenced modulating inducing cells, without affecting normal

Language: Английский

Citations

4
Cold atmospheric plasma control metabolic syndromes via targeting fat mass and obesity-associated protein DOI Creative Commons
Xiaofeng Dai, Shuo Feng, Tian Li

et al.

Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107720 - 107720

Published: March 1, 2025

Both obesity and metabolic disorders are global medical problems. Driven by prolonged inflammation, increases the risk of developing syndromes such as fatty liver, diabetes, cardiovascular diseases cancers. The fat mass obesity-associated protein (FTO) is an m6A demethylase, elevated activity which known to promote pathogenesis many disorders, leading establishment various FTO inhibitors. By combing through intrinsic connections among four primary problems, we attribute their shared pathological cause inflammation. reviewing roles in promoting these current status existing inhibitors treating syndromes, underpinned paramount potential resolving clinical issues targeting urgent need establishing novel with maximized efficacy minimized side effect. Cold atmospheric plasma (CAP) fourth state matter demonstrated associated chronic introducing characteristics CAP, proposed it a possible solution unresolved given its anti-inflammation feature safety. We also emphasized intensive investigations exploring feasibility using CAP that might function FTO.

Language: Английский

Citations

0
Developments in low-temperature plasma applications in Asia DOI

Pankaj Attri,

Kenji Ishikawa, Nozomi Takeuchi

et al.

Reviews of Modern Plasma Physics, Journal Year: 2025, Volume and Issue: 9(1)

Published: Feb. 8, 2025

Language: Английский

Citations

0
Targeting Proteostasis Pathways for Cancer Therapy DOI Creative Commons
Xiaofeng Dai,

Ruohan Lyu,

Guanqun Ge

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101287 - 101287

Published: March 1, 2025

Language: Английский

Citations

0
Cold Atmospheric Plasma Jet Promotes Wound Healing through CK2-coordinated PI3K/AKT and MAPK Signaling Pathways DOI Creative Commons
Pei‐Shan Wu,

Tzu-Hsuan Wong,

Chun-Wei Hou

et al.

Molecular & Cellular Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 100962 - 100962

Published: April 1, 2025

The promising role of cold atmospheric plasma jet (CAPJ) treatment in promoting wound healing has been widely documented therapeutic implications. However, the fact that not all subjects respond equally to CAPJ necessitates investigation underlying cellular mechanisms, which have rarely understood so far. Given is a complex and prolonged process, post-plasma-activated medium (PAM) treated keratinocytes were collected at two time points, 2 hours (receiving) 24 (recovery), for (phospho)proteomic analysis systematically dissect molecular basis CAPJ-promoted healing. receiving (phospho)proteomics datasets, referred point hours, revealed an apparent increase phosphorylation CK2 its-mediated PI3K/AKT MAPK signaling pathways, accompanied by prompted downstream physiological response cell migration. Additionally, incorporating network predicted kinases their direct interactors, we reiterated influenced growth migration, thereby paving way its subsequent processes. Further determining proteome profiles recovery phase, displayed totally different view from had almost no change. up-regulation ROBOs/SLITs expression vesicle trafficking fusion-related proteins, along with abundant presence 14-3-3 family indicated persistent effect PAM on process could potentially promote keratinocyte-fibroblast crosstalk stimulate extracellular matrix (ECM) synthesis upon epithelialization. Consistent patterns, CAPJ-treated tissues indeed showed denser well-organized ECM architecture, implying hastened epithelialization during Collectively, delineated CAPJ-accelerated early late responses, providing valuable insights selection development strategies achieve better outcomes.

Language: Английский

Citations

0
Personalized Plasma Medicine for Cancer: Transforming Treatment Strategies with Mathematical Modeling and Machine Learning Approaches DOI Creative Commons
Viswambari Devi Ramaswamy, Michael Keidar

Applied Sciences, Journal Year: 2023, Volume and Issue: 14(1), P. 355 - 355

Published: Dec. 30, 2023

Plasma technology shows tremendous potential for revolutionizing oncology research and treatment. Reactive oxygen nitrogen species electromagnetic emissions generated through gas plasma jets have attracted significant attention due to their selective cytotoxicity towards cancer cells. To leverage the full of medicine, researchers explored use mathematical models various subsets or approaches within machine learning, such as reinforcement learning deep learning. This review emphasizes application advanced algorithms in adaptive system, paving way precision dynamic Realizing techniques medicine requires efforts, data sharing, interdisciplinary collaborations. Unraveling complex mechanisms, developing real-time diagnostics, optimizing will be crucial harnessing true power oncology. The integration personalized therapies, alongside AI diagnostic sensors, presents a transformative approach treatment with improve outcomes globally.

Language: Английский

Citations

7
Cold atmospheric‐pressure plasma selectively inhibits glioblastoma via DNA damage and AKT dephosphorylation in vitro and in vivo DOI
Yinan Chen,

Xiaolong Qiao,

Changqing Liu

et al.

Plasma Processes and Polymers, Journal Year: 2024, Volume and Issue: 21(5)

Published: Jan. 17, 2024

Abstract In this work, the effects of cold atmospheric‐pressure plasma (CAP) on glioblastoma are evaluated comprehensively. After CAP treatment, U251 cell viability, migration, and invasion functions were inhibited, while an appropriate dose had no inhibitory effect human brain glial line cells. Western blots indicated that expression caspase‐3 was upregulated with ki‐67 downregulated. Moreover, mitochondrial membrane potential decreased, energy metabolisms cells influenced afterward. TUNEL assays comet suggested DNA damage after treatment. Furthermore, as one responses associated pathways, AKT (AKT8 virus oncogene cellular homolog) signaling pathway also in work. The findings raise great promise for clinical applications treatments.

Language: Английский

Citations

2