Exploring the Chemical Constituents and Nutritive Potential of Bee Drone (Apilarnil): Emphasis on Antioxidant Properties

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: 21(5)

Published: Feb. 8, 2024

Abstract A lesser‐known bee product called drone brood homogenate (DBH, apilarnil) has recently attracted scientific interest for its chemical and biological properties. It contains pharmacologically active compounds that may have neuroprotective, antioxidant, fertility‐enhancing, antiviral effects. Unlike other products, the composition of larva is poorly studied. This study analyzed compostion apilarnil using several methods. These included liquid chromatography‐mass spectrometry (LC‐MS/MS) a combination gas chromatography/mass with solid phase micro‐extraction (SPME/GC‐MS). Additionally, antioxidant activity was assessed 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay. assessment showed it 6.3±0.00, 74.67±0.10 %, 3.65±0.32 8.80±1.01 13.16±0.94 8.79±0.49 % pH, moisture, total lipids, proteins, flavonoids, carbohydrates, respectively. LC‐MS/MS analysis molecular networking (GNPS) exhibited 44 compounds, including fatty acids, glycerophospholipids, alcohols, sugars, amino steroids. GC‐MS detected 30 volatile in apilarnil, mainly esters (24 %), ketones (23.84 ethers (15.05 alcohols (11.41 acids (10.06), aldehydes (6.73 amines (5.46), alkene (5.53 %). The measured DPPH an IC 50 179.93±2.46 μg/ml.

Language: Английский

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LC–MS/MS, GC–MS and molecular docking analysis for phytochemical fingerprint and bioactivity of Beta vulgaris L.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 29, 2024

Abstract The plants that we consume in our daily diet and use as a risk preventer against many diseases have biological pharmacological activities. In this study, the phytochemical fingerprint activities of Beta vulgaris L. leaf extract, which are widely consumed Black Sea region, were investigated. parts plant dried an oven at 35 °C then ground into powder. main constituents B. identified by LC–MS/MS GC–MS analyses. Phenolic content, betaxanthin betacyanin levels investigated extracts obtained using three different solvents. activity extract was anti-microbial, anti-mutagenic, anti-proliferative anti-diabetic tests. Anti-diabetic vitro enzyme inhibition in-silico molecular docking performed to confirm activity. LC–MS analysis major proportion p_coumaric acid, vannilin, protecatechuic aldehyde sesamol detected, while essential oils determined hexahydrofarnesyl acetone phytol. Among solvents used, highest extraction efficiency 2.4% methanol extraction, 36.2 mg GAE/g phenolic substance, 5.1 mg/L 4.05 extract. , exhibited broad-spectrum anti-microbial forming zone all tested bacteria, anti-mutagenic range 35.9–61.8% various chromosomal abnormalities. did not exhibit mutagenic, sub-lethal or lethal effects, reducing proliferation Allium root tip cells 21.7%. 50 mg/mL caused 58.9% 55.9% α-amylase α-glucosidase activity, respectively. interactions coumaric vanniline, phytol, compounds with silico between molecules via amino acids determined. Binding energies − 4.3 kcal/mol 6.1 kcal/mol, for it 3.7 5.7 kcal/mol. closely related active contains, therefore studies investigating contents very important. Safe non-toxic can help reduce diseases, such diabetes, serve alternative complement current pharmaceutical practices.

Language: Английский

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Antioxidant Potential and Phenolic Content of Plantago major

Published: Feb. 28, 2025

Objective: This study aimed to evaluate the antioxidant capacity and bioactive compound content of aerial parts Plantago major, a medicinal plant with potential therapeutic applications. Methods: The were extracted using 70% methanol, solvent was evaporated. Total phenolic (TPC) total flavonoid (TFC) quantified. Antioxidant assessed DPPH, ABTS, FRAP assays. Results: DPPH radical scavenging assay showed an IC50 value 127.33 ± 1.07 µg/mL, while ABTS revealed 46.74 2.53 µg/mL. demonstrated reducing power equivalent 44.62 0.61 mg TE/g extract. Conclusion: high major extract contributes significantly its strong activity. These findings suggest application as natural source in pharmaceutical nutraceutical industries.

Language: Английский

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Toxicity of environmentally relevant concentration of esfenvalerate and Taraxacum officinale application to overcome toxicity: A multi-bioindicator in-vivo study

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126111 - 126111

Published: March 1, 2025

Language: Английский

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Phytochemical fingerprint and biological activity of raw and heat-treated Ornithogalum umbellatum

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Aug. 23, 2023

The plants that we use as food in our daily diet and risk preventers against many diseases have biological pharmacological activities. heat treatments applied during the cooking of cause changes phytochemical content bioactivity. In this study, fingerprint activities raw heat-treated extracts Ornithogalum umbellatum L., which is widely consumed Black Sea region, were investigated. bulb leaf parts plant dried an oven at 35 °C then ground into powder. For treatment, was boiled 100 for 20 min. Differences contents determined by ICP-MS, LC-MS/MS, FTIR analysis. Biological activity investigated with antiradical, antimicrobial, antimutagenic antiproliferative tests. way, effect treatment on both O. extract determined. Gallic acid, procateuic acid caffeic found main compounds extract, while presence aldehyde, vanillin kaempferol minor proportions There a significant decrease phenolic compound levels after gallic decreased 92.6%, 90% 84.8%. Significant differences detected macro micro element ICP-MS results. While Cd, Ba Zn increased; Na, Mg, K, Fe, U, Co significantly. spectrum, shifts disappearances observed some vibrations emergence new also treatment. Raw exhibited strong scavenging H2O2 DPPH had broad spectrum antimicrobial property. As result application, regressions antiradicalic, antibacterial antifungal Antimutagenic Allium test loss chromosomal abnormalities 20%, to 7.6%. showed strongest 69.8% unequal distribution chromatin. Similarly, reduced bridges 56.1%, 0.74% almost lost its activity. are closely related major it contains, these reflected Studies investigating very important studies more remarkable. determined, contained, be sensitive.

Language: Английский

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Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 13, 2023

Although the antioxidant properties of Melissa officinalis extract (Mox) are widely known, little work has focused on its protective capacity against heavy metal stress. The primary objective this study was to determine potential Mox mitigate manganese (II) chloride (MnCI2)-induced cyto-genotoxicity using Allium and comet assays. Physiological, genotoxic, biochemical anatomical parameters as well phenolic composition were examined in cepa (L.). Application 1000 µM MnCl2 reduced rooting percentage, root elongation, weight gain, mitotic index levels chlorophyll a b pigments compared control group. However, it increased micronuclei formation, chromosomal abnormality frequencies, tail DNA proline amount, lipid peroxidation level meristematic damage severity. activities superoxide dismutase catalase also increased. Chromosomal aberrations induced by fragment, sticky chromosome, vagrant unequal distribution chromatin bridge. 250 mg/L 500 along with significantly alleviated adverse effects dose dependently. activity bestowed compounds assisted organism combat toxicity. Consequently, exerted remarkable protection toxicity needs be investigated further therapeutic option.

Language: Английский

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Siringaldehit Bazlı Yeni 2,4,6-Triarilpiridin Türevlerinin Antioksidan Aktiviteleri ve Teorik Profili

Düzce Üniversitesi Bilim ve Teknoloji Dergisi, Journal Year: 2024, Volume and Issue: 12(2), P. 981 - 999

Published: April 29, 2024

Siringaldehitten türetilen sekiz adet yeni 2,4,6-triarilpiridin tasarlandı ve tek basamaklı multi-komponent yöntemle sentezlendi. Bu bileşiklerin antioksidan aktiviteleri bilinen referans bileşiklerle kıyaslanarak değerlendirildi. Daha sonra, B3LYP teorisi SVP, TVZP temel setleri kullanılarak, sentezlenen bileşikler için kapsamlı bir teorik kuantum hesaplama yaklaşımı oluşturuldu radikal yakalama potansiyelini tanımlayan Fukui indeksleri adlı elektronik yapı tanımlayıcı parametreler belirlendi. Son olarak, deneysel sonuçlar karşılaştırılarak yapı-etkinlik ilişkisi ortaya konuldu. Sonuç elde edilen aktivite potansiyeli yaklaşımla da desteklendi.

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Investigation of the protective role of Ginkgo biloba L. against phytotoxicity, genotoxicity and oxidative damage induced by Trifloxystrobin

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 27, 2024

Trifloxystrobin (TFS) is a widely used strobilurin class fungicide. Ginkgo biloba L. has gained popularity due to its recognized medicinal and antioxidant properties. The aim of this study was determine whether extract (Gbex) protective role against TFS-induced phytotoxicity, genotoxicity oxidative damage in A. cepa. Different groups were formed from Allium cepa bulbs subjected tap water (control), 200 mg/L Gbex (Gbex1), 400 (Gbex2), 0.8 g/L TFS solution (TFS), + (TFS Gbex1) Gbex2), respectively. phenolic composition alterations the morphological, physiological, biochemical, anatomical parameters evaluated. Rutin, protocatechuic acid, catechin, gallic taxifolin, p-coumaric caffeic epicatechin, syringic acid quercetin most prevalent substances Gbex. Rooting percentage, root elongation, weight gain, chlorophyll b decreased by approximately 50%, 85%, 77%, 55% 70%, respectively, as result treatment compared control. In group, mitotic index fell 28% control but chromosomal abnormalities, micronuclei frequency tail DNA percentage increased. Fragment, vagrant chromosome, sticky uneven chromatin distribution, bridge, vacuole-containing nucleus, reverse polarization irregular mitosis abnormalities observed group. levels proline (2.17-fold) malondialdehyde (2.71-fold), well activities catalase (2.75-fold) superoxide dismutase (2.03-fold) increased comparison TFS-provoked meristematic disorders damaged epidermis cortex cells, flattened cell nucleus thickened wall. combined with relieved all these stress signs dose-dependent manner. This investigation showed that can play caused TFS. results demonstrated had antigenotoxic potential owing high content.

Language: Английский

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Ferruginoside D: A Novel Phenylethanoid from Verbascum leiocarpum

Chemistry & Biodiversity, Journal Year: 2023, Volume and Issue: 20(12)

Published: Nov. 1, 2023

A novel phenylethanoid, ferruginoside D, together with fourteen known compounds were isolated from the Verbascum leiocarpum for first time. Chemical structures of determined by spectroscopic analysis including HR-ESI-MS and NMR spectra. The antioxidant, α-glucosidase inhibitory properties, antiproliferative activities against multiple cell lines (A172, C6, HeLa, A2780, SW620, HT29, Beas2B, RPE, HSF) evaluated in vitro. According to results, iridoids, flavonoids (luteolin apigenin), phenylethanoids (poliumoside isoverbascoside) strong potential also found have cytostatic effects. Furthermore, investigation revealed that luteolin, poliumoside, verbascoside, isoverbascoside, C, ursolic acid show potent alpha-glucosidase activity, while isoverbascoside exhibit substantial antioxidant activity. new compound (ferruginoside D) was moderately active cancer lines, moderate properties.

Language: Английский

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LC–MS/MS phenolic profileand remedial role of Urtica dioica extract against Li2CO3-induced toxicity

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(42), P. 54589 - 54602

Published: Aug. 29, 2024

Language: Английский

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