The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(10), P. 1775 - 1790

Published: Sept. 4, 2023

Abstract The mesencephalic locomotor region (MLR) is a brain stem area whose stimulation triggers graded forward locomotion. How MLR neurons recruit downstream vsx2 + (V2a) reticulospinal (RSNs) poorly understood. Here, to overcome this challenge, we uncovered the locus of in transparent larval zebrafish and show that distinct from nucleus medial longitudinal fasciculus. stimulations reliably elicit locomotion controlled duration frequency. V2a RSNs via projections onto somata pontine retropontine areas, dendrites medulla. High-speed volumetric imaging neuronal activity reveals strongly MLR-coupled are active for steering or swimming, whereas weakly medullary encode frequency component. Our study demonstrates how specific control kinematics suggests conservation motor functions across vertebrates.

Language: Английский

---

Projections of the Diencephalospinal Dopaminergic System to Peripheral Sense Organs in Larval Zebrafish (Danio rerio)

Frontiers in Neuroanatomy, Journal Year: 2018, Volume and Issue: 12

Published: March 19, 2018

Dopaminergic neurons of the descending diencephalospinal system are located in posterior tuberculum zebrafish (Danio rerio), and correspond mammals to A11 group hypothalamus thalamus. In larval zebrafish, they likely only source central dopaminergic projections periphery. Here, we characterized tubercular fibers projecting peripheral sense organs, with a focus on lateral line neuromasts. We labelled identified catecholaminergic their by combining two immunofluorescence techniques, (i) using an antibody against Tyrosine hydroxylase, (ii) GFP transgenic expressing either membrane-anchored track fibers, or Synaptophysin-GFP fusion visualize putative synapses. applied CLARITY method six days old whole larvae stain analyze confocal microscopy. found that all neuromasts receive direct innervation neurons, tracked these detail. addition, anterior ganglia, extensive arborizations around terminal projection field afferent hindbrain medial octavolateralis nucleus. Therefore, may affect information at different processing stages. Additional innervate trigeminal ganglion, observed fine skin arborization patterns similar free sensory nerve endings. also detected potentially innervating inner ear epithelia. A11-type broadly modulate senses. briefly report sympathetic labeled our experiments, developing intestine, swim bladder abdominal organs.

Language: Английский

---

Forebrain Dopamine System Regulates Inner Ear Auditory Sensitivity to Socially Relevant Acoustic Signals

Current Biology, Journal Year: 2019, Volume and Issue: 29(13), P. 2190 - 2198.e3

Published: June 13, 2019

Language: Английский

---

Whole-genome resequencing reveals genetic diversity and selection signals in warm temperate and subtropical Sillago sinica populations

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Sept. 15, 2023

Abstract Background Genetic diversity and heterogeneous genomic signatures in marine fish populations may result from selection pressures driven by the strong effects of environmental change. Nearshore fishes are often exposed to complex environments human activities, especially those with small ranges. However, studies on genetic population signals these species have mostly been based a relatively number markers. As newly recorded Sillaginidae, genetics Sillago sinica fragmented or even absent. Results To address this theoretical gap, we performed whole-genome resequencing 43 S. individuals Dongying (DY), Qingdao (QD) Wenzhou (WZ) obtained 4,878,771 high-quality SNPs. Population analysis showed that was low, but WZ higher than other two populations. Interestingly, three were not strictly clustered within group defined their sampling location an obvious geographic structure signal warm temperate subtropics. With further analysis, warm-temperate exhibited regions related nervous system development, sensory function immune function. subtropical more selective signalling for tolerance stress transduction. Conclusions Genome-wide SNPs provide data support localization The reduction be bottleneck effect. Considering low leads reduced adaptability, conservation efforts monitoring should increased future. Differences between activities changes complexity. This study deepened understanding nearshore provided basis exploring potential mechanisms variation pressures.

Language: Английский

---

Attention and Motivated Response to Simulated Male Advertisement Call Activates Forebrain Dopaminergic and Social Decision-Making Network Nuclei in Female Midshipman Fish

Integrative and Comparative Biology, Journal Year: 2017, Volume and Issue: 57(4), P. 820 - 834

Published: May 24, 2017

Little is known regarding the coordination of audition with decision-making and subsequent motor responses that initiate social behavior including mate localization during courtship. Using midshipman fish model, we tested hypothesis time spent by females attending responding to advertisement call correlated activation a specific subset catecholaminergic (CA) network (SDM) nuclei underlying auditory- driven sexual motivation. In addition, quantified relationship neural between CA SDM in all responders goal providing map functional connectivity circuitry motivated state responsive acoustic cues localization. order make baseline qualitative comparison this brain unmotivated females, made similar correlative who were unresponsive playback. Our results support an important role for dopaminergic neurons periventricular posterior tuberculum ventral thalamus, putative A11 A13 tetrapod homologues, respectively, as well parvocellular preoptic area dorsomedial telencephalon, (laterobasal amygdala homologue) auditory attention appetitive fishes. These findings may also offer insights into function these highly conserved context auditory-driven reproductive across vertebrates.

Language: Английский

---

Galanin immunoreactivity is sexually polymorphic in neuroendocrine and vocal‐acoustic systems in a teleost fish

The Journal of Comparative Neurology, Journal Year: 2019, Volume and Issue: 528(3), P. 433 - 452

Published: Aug. 30, 2019

Abstract Galanin is a peptide that regulates pituitary hormone release, feeding, and reproductive parental care behaviors. In teleost fish, increased galanin expression associated with territorial, reproductively active males. Prior transcriptome studies of the plainfin midshipman ( Porichthys notatus ), highly vocal fish two male morphs follow alternative tactics, show upregulated in preoptic area‐anterior hypothalamus (POA‐AH) nest‐holding, courting type I males during spawning compared to cuckolding II Here, we investigate possible differences immunoreactivity brain both females focus on vocal‐acoustic neuroendocrine networks. We find differ dramatically from number galanin‐expressing somata distribution fibers, especially brainstem nuclei other sensory integration sites also differ, though less extensively, between morphs. Double labeling shows primarily separate populations POA‐AH neurons express nonapeptides arginine‐vasotocin or isotocin, homologues mammalian arginine vasopressin oxytocin are broadly implicated neural mechanisms vertebrate social behavior including morph‐specific actions neurophysiology midshipman. Finally, report small population coexpress neurotransmitter γ‐aminobutyric acid. Together, results indicate likely modulate activity at broad scale, targeted effects motor, systems; unique nonapeptide‐expressing populations; play role male‐specific

Language: Английский

---

Brain Activation Patterns in Response to Conspecific and Heterospecific Social Acoustic Signals in Female Plainfin Midshipman Fish, <b><i>Porichthys notatus</i></b>

Brain Behavior and Evolution, Journal Year: 2018, Volume and Issue: 91(1), P. 31 - 44

Published: Jan. 1, 2018

While the peripheral auditory system of fish has been well studied, less is known about how fish’s brain and central process complex social acoustic signals. The plainfin midshipman fish, <i>Porichthys notatus</i>, become a good species for investigating neural basis communication because production reception signals paramount this species’ reproductive success. Nesting males produce long-duration advertisement calls that females detect localize among noise in intertidal zone to successfully find mates spawn. How female are able discriminate male from environmental other stimuli unknown. Using immediate early gene product cFos as marker activity, we quantified activation ascending pathway exposed conspecific calls, heterospecific white seabass or ambient environment noise. We hypothesized hindbrain nuclei would be activated by general (ambient biotic stimuli) whereas neurons midbrain forebrain selectively calls. show two regions hindbrain, i.e., rostral intermediate division descending octaval nucleus ventral secondary nucleus, did not differ via immunoreactive (cFos-ir) activity when different stimuli. In contrast, showed greater cFos-ir centralis torus semicircularis compared only No difference was observed animals versus However, structures receive input, posterior thalamus anterior tuberal hypothalamus, either Our results suggest higher-order semicircularis, thalamic hypothalamic may necessary discrimination Furthermore, differentially exposure

Language: Английский

---

Melatonin receptor expression in vocal, auditory, and neuroendocrine centers of a highly vocal fish, the plainfin midshipman (Porichthys notatus)

The Journal of Comparative Neurology, Journal Year: 2019, Volume and Issue: 527(8), P. 1362 - 1377

Published: Jan. 9, 2019

Melatonin plays a central role in entraining activity to the day-night cycle vertebrates. Here, we investigate neuroanatomical substrates of melatonin-dependent vocal-acoustic behavior nocturnal and highly vocal teleost fish, plainfin midshipman (Porichthys notatus). Using situ hybridization (ISH) quantitative real-time PCR (qPCR), assess mRNA distribution transcript abundance melatonin receptor subtype 1B (mel1b), shown be important for vocalization fish songbirds. ISH shows robust mel1b expression major nodes auditory networks subpallium, preoptic area (POA), anterior hypothalamus, dorsal thalamus, posterior tuberculum, midbrain torus semicircularis periaqueductal gray, hindbrain. Mel1b label is also abundant secondary targets olfactory, visual, lateral line systems, as well telencephalic regions that have been compared amygdala, extended striatum, septum, hippocampus tetrapods. Q-PCR corroborates throughout brain significant increases morning with nighttime tissue samples inclusive telencephalon POA, but remains stable other regions. Plasma levels show expected increase at night. Our findings support hypothesis melatonin's stimulatory effects on mechanisms mediated, part, by binding vocal, auditory, neuroendocrine centers. Together multiple nuclei sensory results further indicate an pattern comparable birds mammals indicative broad involvement modulation physiology behavior.

Language: Английский

---

Dopaminergic neurons are preferentially responsive to advertisement calls and co-active with social behavior network nuclei in sneaker male midshipman fish

Brain Research, Journal Year: 2018, Volume and Issue: 1701, P. 177 - 188

Published: Sept. 11, 2018

Language: Английский

---

Functional coupling of the mesencephalic locomotor region and V2a reticulospinal neurons driving forward locomotion

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 5, 2022

Abstract Locomotion in vertebrates relies on high brain centers converging onto the mesencephalic locomotor region (MLR). How MLR recruits brainstem reticulospinal neurons (RSNs) to initiate locomotion is incompletely understood due challenge of recording these cells vivo. To tackle this question, we leveraged transparency and genetic accessibility larval zebrafish. In model organism, uncovered locus as a small dorsal coeruleus containing glutamatergic cholinergic neurons. stimulations reliably elicited forward bouts controlled duration speed. We find that elicits by recruiting V2a RSNs pontine retropontine regions, gradually medulla. Remarkably, recruited medulla act maintain encoding speed locomotion. Altogether, our study reveals genetically-identified control kinematics exploration.

Language: Английский

---