Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry DOI
Eric B. Monroe, Suresh P. Annangudi,

Andinet Amare Wadhams

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2018, Volume and Issue: 29(5), P. 923 - 934

Published: April 17, 2018

Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made peptide identification across the Metazoa, for some phyla such as Echinodermata, limited neuropeptides known even fewer have verified on protein level. We employed peptidomic approaches using bioinformatics mass spectrometry (MS) to experimentally confirm 23 prohormones characterize a new prohormone nervous system tissue from Strongylocentrotus purpuratus, purple sea urchin. Ninety-three distinct peptides novel were detected with MS extracts of radial nerves, many which reported or confirmed here first time, representing large-scale study phylum Echinodermata. Many identified their precursor proteins low homology other species/phyla unique By pairing MS, capacity annotate genes is enhanced.

Language: Английский

Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior DOI
Dick R. Nässel, Meet Zandawala

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

Citations

309
Ancient role of vasopressin/oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm DOI Creative Commons

Esther Odekunle,

Dean C. Semmens,

Nataly Martynyuk

et al.

BMC Biology, Journal Year: 2019, Volume and Issue: 17(1)

Published: July 30, 2019

Vasopressin/oxytocin (VP/OT)-type neuropeptides are well known for their roles as regulators of diuresis, reproductive physiology and social behaviour. However, our knowledge functions is largely based on findings from studies vertebrates selected protostomian invertebrates. Little about the VP/OT-type in deuterostomian invertebrates, which more closely related to than protostomes.

Language: Английский

Citations

48
Neuropeptide precursors and neuropeptides in the sea cucumber Apostichopus japonicus: a genomic, transcriptomic and proteomic analysis DOI Creative Commons
Muyan Chen, Alžbeta Talarovičová,

Yingqiu Zheng

et al.

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: June 20, 2019

The sea cucumber Apostichopus japonicus is a foodstuff with very high economic value in China, Japan and other countries south-east Asia. It at the heart of multibillion-dollar industry to meet demand for this product, aquaculture methods facilities have been established. However, there are challenges associated optimization reproduction, feeding growth non-natural environments. Therefore, we need learn more about biology A. japonicus, including processes such as aestivation, evisceration, regeneration albinism. One major classes molecules that regulate physiology behaviour animals neuropeptides, few bioactive peptides already identified japonicus. To facilitate comprehensive investigations neuropeptide function here analysed genomic transcriptomic sequence data proteomic identify precursors neuropeptides species. We 44 transcripts encoding or putative precursors, some instances derived from these were confirmed by mass spectrometry. Furthermore, analysis enabled identification location precursor genes on scaffolds linkage groups (chromosomes) determination gene structure. Many contain homologs bilaterian animals. Precursors thus far only echinoderms identified, L- F-type SALMFamides, AN others. several act modulators neuromuscular activity also identified. discovery large repertoire provides basis experimental studies investigate physiological roles signaling systems Looking ahead, may effects could be harnessed enable improvements economically important

Language: Английский

Citations

43
Ancient role of sulfakinin/cholecystokinin-type signalling in inhibitory regulation of feeding processes revealed in an echinoderm DOI Creative Commons
Ana B. Tinoco, Antón Barreiro‐Iglesias, Luis Alfonso Yañez Guerra

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 7, 2021

Sulfakinin (SK)/cholecystokinin (CCK)-type neuropeptides regulate feeding and digestion in protostomes (e.g. insects) chordates. Here, we characterised SK/CCK-type signalling for the first time a non-chordate deuterostome – starfish Asterias rubens (phylum Echinodermata). In this species, two (ArSK/CCK1, ArSK/CCK2) derived from precursor protein ArSK/CCKP act as ligands an receptor (ArSK/CCKR) these peptides/proteins are expressed nervous system, digestive tube feet, body wall. Furthermore, ArSK/CCK1 ArSK/CCK2 cause dose-dependent contraction of cardiac stomach, foot, apical muscle preparations vitro, injection vivo triggers stomach retraction inhibition onset A. . Thus, evolutionarily ancient role inhibitory regulators feeding-related processes Bilateria has been conserved unusual unique context extra-oral behaviour pentaradial plan echinoderm.

Language: Английский

Citations

37
Somatostatin-type and allatostatin-C–type neuropeptides are paralogous and have opposing myoregulatory roles in an echinoderm DOI Creative Commons
Ya Zhang, Luis Alfonso Yañez Guerra, Ana B. Tinoco

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(7)

Published: Feb. 10, 2022

Somatostatin (SS) and allatostatin-C (ASTC) are inhibitory neuropeptides in chordates protostomes, respectively, which hitherto were identified as orthologs. However, echinoderms have two SS/ASTC-type (SS1 SS2), here, our analysis of sequence data indicates that SS1 is an ortholog ASTC SS2 SS. The occurrence both SS-type ASTC-type provides a unique context to compare their physiological roles. Investigation the expression actions neuropeptide ArSS1 starfish Asterias rubens revealed it causes muscle contraction (myoexcitation), contrasting with myoinhibitory effects ArSS2. Our findings suggest paralogous originated by gene duplication common ancestor Bilateria, only one type being retained protostomes but types echinoderms. Loss may been due functional redundancy regulators processes. Conversely, retention be consequence evolution myoexcitatory role for mediated yet unknown signaling mechanisms.

Language: Английский

Citations

20
Biochemical, Anatomical, and Pharmacological Characterization of Calcitonin-Type Neuropeptides in Starfish: Discovery of an Ancient Role as Muscle Relaxants DOI Creative Commons
Weigang Cai, Chan‐Hee Kim, Hye‐Jin Go

et al.

Frontiers in Neuroscience, Journal Year: 2018, Volume and Issue: 12

Published: June 8, 2018

Calcitonin (CT) is a peptide hormone released by the thyroid gland that regulates blood Ca2+ levels in mammals. The CT gene alternatively spliced, with one transcript encoding and another CT-like neuropeptide calcitonin-gene related (α-CGRP), which powerful vasodilator. Other CT-related peptides vertebrates include adrenomedullin, amylin intermedin, also act as smooth muscle relaxants. evolutionary origin of CT-type has been traced to bilaterian common ancestor protostomes deuterostomes (DH31) identified diuretic some insect species. However, little known about physiological roles other invertebrates. Here we characterized deuterostomian invertebrate – starfish Asterias rubens (Phylum Echinodermata). A precursor cDNA (ArCTP) was sequenced predicted structure (ArCT) derived from ArCTP confirmed using mass spectrometry. distribution mRNA ArCT investigated situ hybridization immunohistochemistry, respectively, revealing stained cells/processes nervous system, digestive system muscular organs, including apical tube feet. Investigation effects synthetic on vitro preparations feet revealed it acts relaxant, causing dose-dependent reversal acetylcholine-induced contraction. Furthermore, relaxant present whole-animal extracts species, Patiria pectinifera, an ortholog named PpCT. Consistent expression pattern A. rubens, RT-qPCR P. pectinifera PpCT more abundant radial nerve cords than tissues/organs analyzed. In conclusion, our findings indicate action relaxants may reflect evolutionarily ancient role neuropeptides can be back deuterostomes.

Language: Английский

Citations

37
Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome DOI Creative Commons
Luis Alfonso Yañez Guerra, Jérôme Delroisse, Antón Barreiro‐Iglesias

et al.

Scientific Reports, Journal Year: 2018, Volume and Issue: 8(1)

Published: May 2, 2018

Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution comparative physiology luqin-type neuropeptide signalling, which has been characterised previously protostomian invertebrates. Phylogenetic analysis indicates that receptors tachykinin-type paralogous probably originated a common ancestor Bilateria. In deuterostomian lineage, signalling lost chordates but interestingly it retained ambulacrarians. Therefore, here for first time an ambulacrarian - starfish Asterias rubens (phylum Echinodermata). A luqin-like with C-terminal RWamide motif (ArLQ; EEKTRFPKFMRW-NH

Language: Английский

Citations

35
Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate DOI Creative Commons
Ya Zhang, Luis Alfonso Yañez Guerra,

Michaela Egertová

et al.

Open Biology, Journal Year: 2020, Volume and Issue: 10(9)

Published: Sept. 1, 2020

Somatostatin (SS) and allatostatin-C (ASTC) are structurally evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals insects, respectively. Here, we report the first molecular functional characterization SS/ASTC-type signalling a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two precursors were identified A. (ArSSP1 ArSSP2) structures derived from these proteins (ArSS1 ArSS2) analysed using mass spectrometry. Pharmacological three cloned receptors (ArSSR1–3) revealed ArSS2, but not ArSS1, acts ligand for all receptors. Analysis ArSS2 expression mRNA situ hybridization immunohistochemistry stained cells/fibres central nervous system, digestive system (e.g. cardiac stomach) body wall its appendages tube feet). Furthermore, vitro pharmacological tests causes dose-dependent relaxation foot stomach preparations, while injection vivo partial eversion stomach. Our findings provide new insights into evolution animal kingdom reveal an ancient role SS-type muscle contractility.

Language: Английский

Citations

32
The neuropeptidomes of the sea cucumbers Stichopus cf. horrens and Holothuria scabra DOI Creative Commons

John Aidan A. Resoles,

Eizadora T. Yu

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 27, 2025

The Philippines is a renowned marine biodiversity hotspot, home to several sea cucumber species with unusual biological traits. Among these, Stichopus cf. horrens notable for its ability undergo rapid body wall liquefaction when stressed, coupled remarkable regenerative abilities. In contrast, Holothuria scabra has one of the most robust walls in cucumbers and thrives many regimes tropics. Despite their intriguing traits, neurobiology chemical diversity these remain underexplored. Neuropeptides are important components an animal's neurobiological toolkit that underlie various physiological behavioral processes. Thus, discovery neuropeptides crucial step understanding molecular underpinnings unique traits cucumbers. Leveraging throughput sensitivity tandem mass spectrometry, we obtained unbiased view endogenous peptidomes radial nerve cord tissues non-model species, H. S. horrens. this work, sequenced 60 mature peptides from were derived 22 precursor proteins, 43 originating 25 proteins nervous tissues. A total seven previously unannotated uncharacterized neuropeptide precursors identified, thereby expanding known animal repertoire. Furthermore, discovered consistent structural features based on type post-translational modifications while pushing forward potentially novel proteolytic processing sites during peptide maturation enriched flanking amino acid residues. Collectively, our results provide preliminary data expand echinoderm through discovery, paving way innovative solutions address global demand echinoderms.

Language: Английский

Citations

0
Identification and characterization of neuropeptides in sea urchin Strongylocentrotus intermedius DOI
Qianqian Li,

Mingya Zhang,

K. Liu

et al.

General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 114716 - 114716

Published: March 1, 2025

Language: Английский

Citations

0