KHDRBS1 regulates the pentose phosphate pathway and malignancy of GBM through SNORD51-mediated polyadenylation of ZBED6 pre-mRNA DOI Creative Commons

Xiaoyu Liu,

Xiaobai Liu,

Weiwei Dong

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(11)

Published: Nov. 8, 2024

Glioblastoma is one of the most common and aggressive primary brain tumors. The aberration metabolism important character GBM cells tightly related to malignancy GBM. We mainly verified regulatory effects KHDRBS1, SNORD51 ZBED6 on pentose phosphate pathway malignant biological behavior in glioblastoma cells, such as proliferation, migration invasion. KHDRBS1 were upregulated tissues cells. But had opposite tendency may improve stability by binding SNORD51, thus elevating expression SNORD51. More importantly, can competitively bind WDR33 with 3'UTR pre-mRNA which inhibit 3' end processing pre-mRNA, thereby inhibiting mRNA. inhibited transcription G6PD promoter region G6PD. Therefore, KHDRBS1/SNORD51/ZBED6 performs an part regulating influence providing new insights potential targets for treatment

Language: Английский

SnoRNAs: the promising targets for anti-tumor therapy DOI Creative Commons
Xiao Hu, Wanlin Cui, Min Liu

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(11), P. 101064 - 101064

Published: Aug. 5, 2024

Recently, snoRNAs have transcended the genomic "noise" to emerge as pivotal molecular markers due their essential roles in tumor progression. Substantial evidence indicates a strong association between and critical clinical features such pathology drug resistance. Historically, snoRNA research has concentrated on two classical mechanisms: 2'-O-ribose methylation pseudouridylation. This review specifically summarizes novel regulatory mechanisms functional patterns of tumors, encompassing transcriptional, post-transcriptional, post-translational regulation. We further discuss synergistic effect host genes (SNHGs) More importantly, extensively contribute development cell resistance oncogenes or suppressor genes. Accordingly, we provide comprehensive diagnosis treatment associated with explore significant potential targets.

Language: Английский

Citations

3
Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells DOI

Ying Zhan,

Wei Qiao,

Bolong Yi

et al.

Oncogene, Journal Year: 2022, Volume and Issue: 41(40), P. 4512 - 4523

Published: Aug. 29, 2022

Language: Английский

Citations

14
The circular RNA Rap1b promotes Hoxa5 transcription by recruiting Kat7 and leading to increased Fam3a expression, which inhibits neuronal apoptosis in acute ischemic stroke DOI Creative Commons
Liang Zhang, Fang-Fang Zhang, Lin Zhao

et al.

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 18(10), P. 2237 - 2237

Published: Jan. 1, 2023

Circular RNAs can regulate the development and progression of ischemic cerebral disease. However, it remains unclear whether they play a role in acute stroke. To investigate circular RNA Rap1b (circRap1b) stroke, this study we established an vitro model ischemia hypoxia by subjecting HT22 cells to oxygen glucose deprivation mouse occluding right carotid artery. We found that circRap1b expression was remarkably down-regulated hippocampal tissue cell model. In addition, Hoxa5 strongly up-regulated response overexpression. low hippocampus HT22-AIS cells, inhibited apoptosis. Importantly, promoted transcription recruiting acetyltransferase Kat7 induce H3K14ac modification promoter region. regulated neuronal apoptosis activating Fam3a, apoptosis-related protein. These results suggest regulates expression, subsequently Fam3a ultimately inhibiting Lastly, explored potential clinical relevance vivo. Taken together, these findings demonstrate mechanism which inhibits

Language: Английский

Citations

8
U3 snoRNA‐mediated degradation of ZBTB7A regulates aerobic glycolysis in isocitrate dehydrogenase 1 wild‐type glioblastoma cells DOI Creative Commons

Weiwei Dong,

Yunhui Liu, Ping Wang

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2023, Volume and Issue: 29(10), P. 2811 - 2825

Published: April 17, 2023

Abstract Aims The isocitrate dehydrogenase (IDH) phenotype is associated with reprogrammed energy metabolism in glioblastoma (GBM) cells. Small nucleolar RNAs (snoRNAs) are known to exert an important regulatory role the of tumor purpose this study was investigate C/D box snoRNA U3 and transcription factor zinc finger BTB domain‐containing 7A (ZBTB7A) regulation aerobic glycolysis proliferative capacity IDH1 wild‐type (IDH1 WT ) GBM Methods Quantitative reverse PCR western blot assays were utilized detect ZBTB7A expression. promoter methylation status analyzed via bisulfite sequencing methylation‐specific PCR. Seahorse XF stress assays, lactate production glucose consumption measurement cell viability proliferation Results We found that hypomethylation CpG island region led upregulation expression cells, knockdown suppressed ability small nucleolar‐derived RNA (sdRNA) U3‐miR, a fragment produced by U3, able bind ZBTB4 3′UTR reduce mRNA stability, thereby downregulating protein Furthermore, transcriptionally inhibited hexokinase 2 (HK2) A (LDHA), which key enzymes glycolysis, directly binding HK2 LDHA regions, forming U3/ZBTB7A/HK2 pathway regulates Conclusion enhances cells axis.

Language: Английский

Citations

7
Non-coding transcriptome profiles in clear-cell renal cell carcinoma DOI
Tereza Tesařová, Ondřej Fiala, Milan Hora

et al.

Nature Reviews Urology, Journal Year: 2024, Volume and Issue: 22(3), P. 151 - 174

Published: Sept. 6, 2024

Language: Английский

Citations

2
TEP SNORD12B, SNORA63, and SNORD14E as novel biomarkers for hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC) DOI Creative Commons
Xuan Zhao,

Guanxuan Chen,

Yawen Wu

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 2, 2024

Abstract Purpose The alterations of RNA profile in tumor-educated platelets (TEPs) have been described as a novel biosource for cancer diagnostics. This study aimed to explore the potential snoRNAs TEP biomarkers diagnostics hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC). Methods Platelets were isolated using low-speed centrifugation and subjected quantitative polymerase chain reaction (qPCR) detection. Results Down-regulated SNORD12B SNORD14E well up-regulated SNORA63 identified from HBV-related HCC, which could act diagnostic HCC early disease. Besides, SNORD12B, SNORD14E, facilitate performance AFP achieve favorable efficiency when combined with platelet parameters. Conclusions Aberrant expression SNORA63, TEPs serve non-invasive diagnosis.

Language: Английский

Citations

2
SNORD17-mediated KAT6B mRNA 2’-O-methylation regulates vasculogenic mimicry in glioblastoma cells DOI
Jingyi Cui, Xiaobai Liu,

Weiwei Dong

et al.

Cell Biology and Toxicology, Journal Year: 2023, Volume and Issue: 39(6), P. 2841 - 2860

Published: April 14, 2023

Language: Английский

Citations

6
FXR1 stabilizes SNORD63 to regulate blood-tumor barrier permeability through SNORD63 mediated 2’-O-methylation of POU6F1 DOI

Chan-chan Liang,

Bei Zhai, Wei Deng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130642 - 130642

Published: March 7, 2024

Language: Английский

Citations

1
Alternative polyadenylation regulates the translation of metabolic and inflammation-related proteins in adipose tissue of gestational diabetes mellitus DOI Creative Commons
Bingnan Chen,

Xu-Yang Chen,

Ruohan Hu

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2024, Volume and Issue: 23, P. 1298 - 1310

Published: March 15, 2024

In gestational diabetes mellitus (GDM), adipose tissue undergoes metabolic disturbances and chronic low-grade inflammation. Alternative polyadenylation (APA) is a post-transcriptional modification mechanism that generates mRNA with variable lengths of 3' untranslated regions (3'UTR), it associated inflammation metabolism. However, the role APA in GDM has not been well characterized. this study, we conducted transcriptomic proteomic sequencing on subcutaneous omental tissues from both control patients. Using Dapars, novel quantitative algorithm, delineated landscape tissue, revealing significant 3'UTR elongation mRNAs group. Omental exhibited correlation between elongated 3'UTRs reduced translation levels genes related to metabolism Validation experiments THP-1 derived macrophages (TDMs) demonstrated impact by overexpressing long short isoforms representative gene LRRC25. Additionally, LRRC25 was validated suppress proinflammatory polarization TDMs. Further exploration revealed two underexpressed trans-acting factors, CSTF3 PPP1CB, tissue. conclusion, study provides preliminary insights into Reduced regulation may contribute disorders downregulating levels. These findings advance our understanding molecular mechanisms underlying GDM-associated changes.

Language: Английский

Citations

1
Comprehensive Characterization of the Regulatory Landscape of Adrenocortical Carcinoma: Novel Transcription Factors and Targets Associated with Prognosis DOI Open Access
João C. D. Muzzi, Jessica Maria Magno,

Jean S. Souza

et al.

Cancers, Journal Year: 2022, Volume and Issue: 14(21), P. 5279 - 5279

Published: Oct. 27, 2022

We reconstructed a transcriptional regulatory network for adrenocortical carcinoma (ACC) using transcriptomic and clinical data from The Cancer Genome Atlas (TCGA)-ACC cohort. investigated the association of units (regulons) with overall survival, molecular phenotypes, immune signatures. annotated ACC regulons cancer hallmarks assessed single sample regulon activities in European Network Study Adrenal Tumors (ENSAT) found 369 associated survival subdivided them into four clusters: RC1 RC2, good prognosis, RC3 RC4, worse outcomes. were highly correlated 'Steroid Phenotype,' while RC2 RC4 proliferation signature. selected two regulons, NR5A1 (steroidogenic factor 1, SF-1) CENPA (Centromeric Protein A), that consistently further downstream analyses. was primary regulator MKI-67 (a marker KI-67), is well-described transcription (TF) tumorigenesis. also ZBTB4 (Zinc finger BTB domain-containing protein 4) regulon, which negatively our network, druggable anti-tumorigenic TF. anticipate may be used as reference investigations concerning complex interactions tumors.

Language: Английский

Citations

6