Biosensors,
Journal Year:
2022,
Volume and Issue:
12(7), P. 503 - 503
Published: July 9, 2022
Food
allergy
has
been
indicated
as
the
most
frequent
adverse
reaction
to
food
ingredients
over
past
few
years.
Since
only
way
avoid
occurrence
of
allergic
phenomena
is
eliminate
allergenic
foods,
it
essential
have
complete
and
accurate
information
on
components
foodstuff.
In
this
framework,
mandatory
crucial
provide
fast,
cost-effective,
affordable,
reliable
analysis
methods
for
screening
specific
allergen
content
in
products.
This
review
reports
research
advancements
concerning
detection,
involving
electrochemical
biosensors.
It
focuses
sensing
strategies
evidencing
different
types
recognition
elements
such
antibodies,
nucleic
acids,
cells,
among
others,
nanomaterial
role,
several
techniques
involved
last,
but
not
least,
ad
hoc
electrodic
surface
modification
approaches.
Moreover,
a
selection
recent
sensors
detection
are
reported
critically
analyzed
terms
sensors’
analytical
performances.
Finally,
advantages,
limitations,
potentialities
practical
applications
biosensors
allergens
discussed.
Engineering in Life Sciences,
Journal Year:
2022,
Volume and Issue:
22(3-4), P. 319 - 333
Published: Jan. 13, 2022
Since
the
invention
of
first
biosensors
70
years
ago,
they
have
turned
into
valuable
and
versatile
tools
for
various
applications,
ranging
from
disease
diagnosis
to
environmental
monitoring.
Traditionally,
antibodies
been
employed
as
capture
probes
in
most
biosensors,
owing
their
innate
ability
bind
target
with
high
affinity
specificity,
are
still
considered
gold
standard.
Yet,
resulting
immunosensors
often
suffer
considerable
limitations,
which
mainly
ascribed
antibody
size,
conjugation
chemistry,
stability,
costs.
Over
past
decade,
aptamers
emerged
promising
alternative
presenting
some
advantages
over
existing
constraints
immunosensors,
well
new
biosensing
concepts.
Herein,
we
review
employment
platforms,
addressing
main
aspects
biosensor
design
mechanism.
We
also
aim
compare
both
probe
classes
theoretical
experimental
perspectives.
highlight
that
such
comparisons
not
straightforward,
these
two
families
should
be
necessarily
perceived
competing
but
rather
complementary.
We,
thus,
elaborate
on
combined
use
hybrid
schemes
benefiting
each
biorecognition
element.
Molecules,
Journal Year:
2023,
Volume and Issue:
28(9), P. 3777 - 3777
Published: April 27, 2023
Electrochemical
biosensors
are
known
as
analytical
tools,
guaranteeing
rapid
and
on-site
results
in
medical
diagnostics,
food
safety,
environmental
protection,
life
sciences
research.
Current
research
focuses
on
developing
sensors
for
specific
targets
addresses
challenges
to
be
solved
before
their
commercialization.
These
typically
include
the
lowering
of
limit
detection,
widening
linear
concentration
range,
analysis
real
samples
a
environment
comparison
with
standard
validation
method.
Nowadays,
functional
nanomaterials
designed
applied
electrochemical
biosensing
support
all
these
challenges.
This
review
will
address
integration
development
diagnosis
viral
infections,
such
COVID-19,
middle
east
respiratory
syndrome
(MERS),
influenza,
hepatitis,
human
immunodeficiency
virus
(HIV),
dengue,
among
others.
The
role
relevance
nanomaterial,
type
biosensor,
technique
adopted
discussed.
Finally,
critical
issues
applying
laboratory
samples,
future
perspectives,
commercialization
aspects
detection
analyzed.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(43), P. 58405 - 58416
Published: Oct. 16, 2024
Apolipoprotein
A1
(Apo-A1)
is
a
well-recognized
biomarker
in
tissues,
closely
associated
with
cardiovascular
diseases
such
as
atherosclerosis,
coronary
artery
disease,
and
heart
failure.
However,
existing
methods
for
Apo-A1
determination
are
limited
by
costly
equipment
intricate
operational
procedures.
Given
the
distinct
advantages
of
electrochemical
immunosensors,
including
affordability
high
sensitivity,
along
unique
attributes
nanobodies
(Nbs),
enhanced
specificity
better
tissue
permeability,
we
developed
an
immunosensor
detection
utilizing
Nb
technology.
In
our
study,
Ce-MOF@AuNPs
nanocomposites
were
synthesized
using
ultrasonic
applied
to
modify
glassy
carbon
electrode.
The
Nb6,
screened
from
immunized
phage
library,
was
immobilized
onto
nanocomposite
material,
establishing
robust
binding
interaction
Apo-A1.
recorded
peak
current
values
demonstrated
logarithmic
increase
corresponding
concentrations
ranging
1
100,000
pg/mL,
limit
36
fg/mL.
Additionally,
immunosensors
selectivity,
good
stability,
reproducibility.
Our
methodology
also
effectively
utilized
serum
sample
analysis,
showing
performance
clinical
assessments.
This
represents
promising
tool
detection,
significant
potential
advancing
disease
diagnostics.
