Multi-dimensional deep learning drives efficient discovery of novel neuroprotective peptides from walnut protein isolates

Food & Function, Journal Year: 2023, Volume and Issue: 14(15), P. 6969 - 6984

Published: Jan. 1, 2023

A deep learning model named MiCNN–LSTM was designed for the rapid screening of multifunctional neuroprotective peptides in this paper. 4 Novel were selected from walnut protein and validated by d -gal-induced mice.

Language: Английский

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Effects of an Akt-activating peptide obtained from walnut protein degradation on the prevention of memory impairment in mice

Food & Function, Journal Year: 2024, Volume and Issue: 15(4), P. 2115 - 2130

Published: Jan. 1, 2024

Akt acts as a central protein influencing multiple pathologies in neurodegenerative diseases including AD and PD, using activators is promising management strategy. The current study characterized the effects of an Akt-activating peptide (Glu-Pro-Glu-Val-Leu-Pro, EPEVLR) obtained from walnut degradation on D-gal-induced memory impairment mice. EPEVLR was by hydrolysis proteins, identification sequences, screening for molecular docking sequentially. MWM test mice indicated that oral administration (80, 200 400 mg per kg day) significantly (p < 0.05) reversed impairment. WB tests mouse hippocampus confirmed could activate promoting its phosphorylation. In addition, further characterization (including TEM, ELISA, immunohistochemistry) related to phosphorylation showed lower Aβ p-tau levels, well more autophagosomes than those model group. Moreover, treatment increased Lactobacillus abundance reduced Helicobacter gut microbiome caused up-regulation SCFAs down-regulation LPS serum metabolites. Therefore, ingestion cognitive symptoms, possibly activation regulation intestinal flora pathway. Consumption EPEVLR-containing diet beneficial treating dysfunction.

Language: Английский

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Lycium ruthenicum Murray derived exosome-like nanovesicles inhibit Aβ-induced apoptosis in PC12 cells via MAPK and PI3K/AKT signaling pathways

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134309 - 134309

Published: July 31, 2024

Language: Английский

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Effects of walnut kernel pellicle on the composition and properties of enzymatic hydrolysates of walnut meal by peptidomics and bioinformatics

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(1)

Published: Jan. 1, 2025

Abstract The purpose of this article is to investigate the effects walnut ( Juglans regia L.) kernel pellicle on composition and properties enzymatic hydrolysis products meal using peptidomics bioinformatics. In study, a total 3423 peptide sequences were identified in peeled protein hydrolysates (PWPH) unpeeled (UWPH). Due presence pellicle, enzyme cleavage sites alkaline proteases precursor proteins altered, resulting differences number length peptides obtained. Principal component analysis indicates significant between PWPH UWPH. Combined with bioinformatics analysis, it was shown that peeling improved release peptides, formed more bioactive reduced allergenicity, water solubility. Seven acetylcholinesterase (AChE) inhibitory activity identified, Val‐Gly‐Ala‐Pro‐Phe‐Asp‐Gly‐Ala (VGAPFDGA) has strongest an IC 50 0.38 ± 0.01 mg/mL. These results confirmed could greatly change hydrolysates, seven novel reported showed AChE activity.

Language: Английский

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A breakthrough computational strategy for efficient enzymatic digestion of walnut protein to prepare antioxidant peptides

Food Chemistry, Journal Year: 2025, Volume and Issue: 476, P. 143311 - 143311

Published: Feb. 14, 2025

Language: Английский

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An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1298, P. 342419 - 342419

Published: Feb. 26, 2024

Language: Английский

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Advances in molecular mechanisms and therapeutic strategies for central nervous system diseases based on gut microbiota imbalance

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Central nervous system (CNS) diseases pose a serious threat to human health, but the regulatory mechanisms and therapeutic strategies of CNS need be further explored. It has been demonstrated that gut microbiota (GM) is closely related disease. GM structure disorders, abnormal microbial metabolites, intestinal barrier destruction elevated inflammation exist in patients with promote development diseases. More importantly, remodeling alleviates pathology some extent. Here, we have summarized mechanism potential treatment for repair based on regulation, aiming provide safer more effective from perspective regulation. The abundance composition associated On basis in-depth analysis changes mice disease, as well its strategies, such probiotics, prebiotics, FMT, may used regulate balance affect thereby promoting recovery

Language: Английский

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Response Surface Methodology Optimization of Exosome-like Nanovesicles Extraction from Lycium ruthenicum Murray and Their Inhibitory Effects on Aβ-Induced Apoptosis and Oxidative Stress in HT22 Cells

Foods, Journal Year: 2024, Volume and Issue: 13(20), P. 3328 - 3328

Published: Oct. 20, 2024

Exosome-like nanovesicles (ELNs) derived from plants are nanoscale vesicles isolated edible plant sources. Lycium ruthenicum Murray (LRM) has garnered growing attention for its dietary value and therapeutic benefits. In this study, a PEG6000-based method was developed to isolate LRM-ELNs. Response surface methodology (RSM) used optimize the extraction conditions obtain optimal efficiency. When PEG6000 concentration at 11.93%, relative centrifugal force 9720 g, incubation time 21.12 h, maximum LRM-ELN yield 4.24 g/kg. This optimization process yielded LRM-ELNs with particle size of 114.1 nm charge −6.36 mV. Additionally, mitigated Aβ-induced apoptosis in HT22 cells by enhancing mitochondrial membrane potential (MMP), lowering Bax/Bcl-2 ratio, reducing Cleaved Caspase-3 expression. Furthermore, alleviated oxidative stress promoting nuclear translocation Nrf2 upregulating expression HO-1 NQO1. These findings indicate that exert protective effects against damage may be considered as supplement Alzheimer’s disease prevention.

Language: Английский

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Advances on resources, biosynthesis pathway, bioavailability, bioactivity, and application of corylifolinin

Future Postharvest and Food, Journal Year: 2024, Volume and Issue: 1(1), P. 82 - 103

Published: March 1, 2024

Abstract Corylifolinin, also known as isobavachalcone, is a flavonoid compound isolated from the seeds of Psoralea corylifolia L. With rich array biological activities, corylifolinin has emerged versatile therapeutic agent. It demonstrated notable antioxidant, anti‐inflammatory, anti‐tumor, anti‐bacterial, and anti‐viral properties. Moreover, holds promise in enhancing liver function, cardiovascular health, providing neuroprotection. Its applications span range medical conditions, including hepatitis, disease, tumors, inflammation. Herein, this comprehensive review delves into various aspects corylifolinin, encompassing its sources, biosynthetic pathways, biotransformation, physicochemical properties, absorption, assimilation, metabolism, excretion. explores molecular mechanisms underlying diverse bioactivities cellular animal models. Furthermore, we present insights clinical trials, human studies, toxicological profiles, safety considerations, marketed products, patents related to corylifolinin. This compilation aims serve valuable reference for both development application corylifolinin‐based therapies.

Language: Английский

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Plant-Derived Proteins/Peptides for the Management of Alzheimer's Disease

Current Nutrition & Food Science, Journal Year: 2024, Volume and Issue: 21(1), P. 35 - 45

Published: April 30, 2024

: Alzheimer's Disease (AD) is a neurological condition that worsens over time and has gradual start. It significant impact on the well-being of human beings. Peptides are substances produced from plants have been revealed to inhibit progression disease disorders, making them promising strategy for prevention disease. Nevertheless, because enormously convoluted pathophysiology recognition majority research action plant-derived peptides solitary instead sufficiently comprehensive, development implementation Plant-derived Alzheimer-prevention (PADPs) constrained. The molecular pathways PADPs, AD-prevention activity, some perspectives current advanced technologies discussed in this review. Additionally, review provides summary techniques available obtaining as well vitro vivo protocols evaluating activity PADPs preventing fundamental concepts manufacturing utilization developed study.

Language: Английский

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