ACS Biomaterials Science & Engineering,
Journal Year:
2019,
Volume and Issue:
5(9), P. 4167 - 4182
Published: July 29, 2019
Liver
tissue
engineering
aims
at
the
possibility
of
reproducing
a
fully
functional
organ
for
treatment
acute
and
chronic
liver
disorders.
Approaches
in
this
field
endeavor
to
replace
transplantation
(gold
standard
diseases
clinical
setting)
with
vitro
developed
constructs.
However,
complexity
microarchitecture
functionality
along
limited
supply
cellular
components
pose
numerous
challenges.
This
review
provides
comprehensive
outlook
onto
how
physicochemical,
mechanobiological,
spatiotemporal
aspects
substrates
could
be
tuned
address
current
challenges
field.
We
also
highlight
strategic
advancements
made
so
far
development
artificial
tissue.
further
showcase
currently
available
prototypes
research
trials,
which
shows
hope
future
engineering.
Nature Communications,
Journal Year:
2019,
Volume and Issue:
10(1)
Published: Dec. 11, 2019
Organoids
have
extensive
therapeutic
potential
and
are
increasingly
opening
up
new
avenues
within
regenerative
medicine.
However,
their
clinical
application
is
greatly
limited
by
the
lack
of
effective
GMP-compliant
systems
for
organoid
expansion
in
culture.
Here,
we
envisage
that
use
extracellular
matrix
(ECM)
hydrogels
derived
from
decellularized
tissues
(DT)
can
provide
an
environment
capable
directing
cell
growth.
These
gels
possess
biochemical
signature
tissue-specific
ECM
translation.
Gels
porcine
small
intestine
(SI)
mucosa/submucosa
enable
formation
growth
endoderm-derived
human
organoids,
such
as
gastric,
hepatic,
pancreatic,
SI.
be
used
a
tool
direct
derivation,
with
stable
transcriptomic
signature,
vivo
delivery.
The
development
these
ECM-derived
opens
organoids
to
clinically.
Communications Biology,
Journal Year:
2021,
Volume and Issue:
4(1)
Published: Dec. 10, 2021
Organoids-cellular
aggregates
derived
from
stem
or
progenitor
cells
that
recapitulate
organ
function
in
miniature-are
of
growing
interest
developmental
biology
and
medicine.
Organoids
have
been
developed
for
organs
tissues
such
as
the
liver,
gut,
brain,
pancreas;
they
are
used
surrogates
to
study
a
wide
range
questions
basic
biology,
genetic
disorders,
therapies.
However,
many
organoids
reported
date
cultured
Matrigel,
which
is
prepared
secretion
Engelbreth-Holm-Swarm
mouse
sarcoma
cells;
Matrigel
complex
poorly
defined.
This
complexity
makes
it
difficult
elucidate
Matrigel-specific
factors
governing
organoid
development.
In
this
review,
we
discuss
promising
Matrigel-free
methods
generation
maintenance
use
decellularized
extracellular
matrix
(ECM),
synthetic
hydrogels,
gel-forming
recombinant
proteins.
Microsystems & Nanoengineering,
Journal Year:
2020,
Volume and Issue:
6(1)
Published: Oct. 4, 2020
Innovations
in
biomaterials
and
stem
cell
technology
have
allowed
for
the
emergence
of
novel
three-dimensional
(3D)
tissue-like
structures
known
as
organoids
spheroids.
As
a
result,
compared
to
conventional
2D
culture
animal
models,
these
complex
3D
improved
accuracy
facilitated
vitro
investigations
human
diseases,
development,
personalized
medical
treatment.
Due
rapid
progress
this
field,
numerous
spheroid
organoid
production
methodologies
been
published.
However,
many
current
techniques
are
limited
by
complexity,
throughput,
reproducibility.
Microfabricated
microscale
platforms
(e.g.,
microfluidics
microprinting)
shown
promise
address
some
limitations
both
generation.
microfluidic
devices
improve
nutrient
delivery
exchange
arrayed
size-controlled
areas
that
yield
more
uniform
spheroids
higher
throughput
at
lower
cost.
In
review,
we
discuss
most
recent
methods,
challenges
currently
faced
production,
microfabricated
applications
improving
Specifically,
focus
on
how
microfabrication
methods
such
lithography,
microcontact
printing,
systems
can
advance
medicine.
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: July 10, 2020
Abstract
The
recent
demonstration
that
primary
cells
from
the
liver
can
be
expanded
in
vitro
as
organoids
holds
enormous
promise
for
regenerative
medicine
and
disease
modelling.
use
of
three-dimensional
(3D)
cultures
based
on
ill-defined
potentially
immunogenic
matrices,
however,
hampers
translation
organoid
technology
into
real-life
applications.
We
here
chemically
defined
hydrogels
efficient
derivation
both
mouse
human
hepatic
organoids.
Organoid
growth
is
found
to
highly
stiffness-sensitive,
a
mechanism
independent
acto-myosin
contractility
requiring
instead
activation
Src
family
kinases
(SFKs)
yes-associated
protein
1
(YAP).
Aberrant
matrix
stiffness,
other
hand,
results
compromised
proliferative
capacity.
Finally,
we
demonstrate
establishment
biopsy-derived
without
animal
components
at
any
step
process.
Our
approach
thus
opens
up
exciting
perspectives
protocols
organoid-based
medicine.
Bioactive Materials,
Journal Year:
2021,
Volume and Issue:
9, P. 198 - 220
Published: July 10, 2021
The
construction
of
biomimetic
vasculatures
within
the
artificial
tissue
models
or
organs
is
highly
required
for
conveying
nutrients,
oxygen,
and
waste
products,
improving
survival
engineered
tissues
in
vitro.
In
recent
times,
remarkable
progress
utilizing
hydrogels
understanding
vascular
biology
have
enabled
creation
three-dimensional
(3D)
composed
complex
systems.
this
review,
we
give
an
emphasis
on
utilization
their
advantages
vascularization
tissues.
Initially,
significance
elements
regeneration
mechanisms
vascularization,
including
angiogenesis
vasculogenesis,
are
briefly
introduced.
Further,
highlight
importance
as
microenvironments
fabricating
vascularized
organs,
terms
tunable
physical
properties,
high
similarity
physiological
environments,
alternative
shaping
mechanisms,
among
others.
Furthermore,
discuss
such
hydrogels-based
various
applications,
regeneration,
drug
screening,
organ-on-chips.
Finally,
put
forward
key
challenges,
multifunctionalities
hydrogels,
selection
suitable
cell
phenotype,
sophisticated
engineering
techniques,
clinical
translation
behind
development
with
towards
future
development.
Regenerative Therapy,
Journal Year:
2021,
Volume and Issue:
18, P. 88 - 96
Published: May 18, 2021
Decellularized
extracellular
matrix
(dECM)
is
widely
used
in
regenerative
medicine
as
a
scaffold
material
due
to
its
unique
biological
activity
and
good
biocompatibility.
Hydrogel
three-dimensional
network
structure
polymer
with
high
water
content
swelling
that
can
simulate
the
environment
of
human
tissues,
has
biocompatibility,
exchange
nutrients,
oxygen,
waste
cells.
At
present,
hydrogel
ideal
for
tissue
engineering.
In
recent
years,
rapid
development
theory
technology
progress
use
dECM
form
hydrogels
have
attracted
considerable
attention
an
innovative
method
engineering
medicine.
This
article
introduces
classification
hydrogels,
focuses
on
history
formation
source
dECM,
application
commercial
materials.
Small,
Journal Year:
2023,
Volume and Issue:
19(25)
Published: March 16, 2023
Abstract
Over
the
past
decade,
stem
cell‐
and
tumor‐derived
organoids
are
most
promising
models
in
developmental
biology
disease
modeling,
respectively.
The
matrix
is
one
of
three
main
elements
construction
an
organoid
important
module
its
extracellular
microenvironment.
However,
source
currently
available
commercial
matrix,
Matrigel,
limits
application
clinical
medicine.
It
worth
investigating
whether
original
decellularized
(dECM)
can
be
exploited
as
improving
very
important.
In
this
review,
tissue
decellularization
protocols
characteristics
methods,
mechanical
support
biological
cues
extraccellular
(ECM),
methods
for
multifunctional
dECM
responsive
hydrogel,
potential
applications
functional
summarized.
addition,
some
expectations
provided
applications.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Feb. 8, 2024
With
the
increase
in
weight
and
age
of
population,
consumption
tobacco,
inappropriate
foods,
reduction
sports
activities
recent
years,
bone
joint
diseases
such
as
osteoarthritis
(OA)
have
become
more
common
world.
From
past
until
now,
various
treatment
strategies
(e.g.,
microfracture
treatment,
Autologous
Chondrocyte
Implantation
(ACI),
Mosaicplasty)
been
investigated
studied
for
prevention
this
disease.
However,
these
methods
face
problems
being
invasive,
not
fully
repairing
tissue,
damaging
surrounding
tissues.
Tissue
engineering,
including
cartilage
tissue
is
one
minimally
innovative,
effective
regeneration
damaged
cartilage,
which
has
attracted
attention
scientists
fields
medicine
biomaterials
engineering
several
years.
Hydrogels
different
types
with
diverse
properties
desirable
candidates
treating
tissue.
They
can
cover
most
shortcomings
other
cause
least
secondary
damage
to
patient.
Besides
using
hydrogels
an
ideal
strategy,
new
drug
delivery
methods,
targeted
through
mechanical
signaling,
interesting
strategies.
In
study,
we
review
discuss
hydrogels,
used
hydrogel
manufacturing,
cartilage-targeting
delivery,
mechanosignaling
modern
treatment.
Cancers,
Journal Year:
2020,
Volume and Issue:
12(10), P. 2727 - 2727
Published: Sept. 23, 2020
One
of
the
promising
directions
in
personalized
medicine
is
use
three-dimensional
(3D)
tumor
models
such
as
spheroids
and
organoids.
Spheroids
organoids
are
cultures
cells
that
can
be
obtained
from
patient
tissue
and,
using
high-throughput
methods,
provide
a
suitable
therapy
for
patient.
These
3D
most
types
tumors,
which
provides
opportunities
creation
biobanks
with
appropriate
materials
used
to
screen
drugs
facilitate
development
therapeutic
agents.
It
should
noted
would
expand
understanding
biology
its
microenvironment,
help
develop
new
vitro
platforms
drug
testing
create
strategies.
In
this
review,
we
discuss
spheroid
organoid
models,
their
advantages
disadvantages,
evaluate
medicine.
