Tissue Engineering and Regenerative Medicine, Journal Year: 2023, Volume and Issue: 20(5), P. 661 - 670
Published: May 9, 2023
Language: Английский
Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522
Published: Dec. 21, 2022
Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.
Language: Английский
Citations
275
Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100530 - 100530
Published: Dec. 28, 2022
In view of their low immunogenicity, biomimetic internal environment, tissue- and organ-like physicochemical properties, functionalization potential, decellularized extracellular matrix (dECM) materials attract considerable attention are widely used in tissue engineering. This review describes the composition matrices role stem-cell differentiation, discusses advantages disadvantages existing decellularization techniques, presents methods for characterization scaffolds. addition, we discuss progress use dECMs cartilage, skin, nerve, muscle repair transplantation or regeneration different whole organs (e.g., kidneys, liver, uterus, lungs, heart), summarize shortcomings using organ after refunctionalization, examine corresponding future prospects. Thus, present helps to further systematize application functionalized tissue/organ keep researchers up date on recent dECM usage.
Language: Английский
Citations
71
Exploration, Journal Year: 2024, Volume and Issue: unknown
Published: April 11, 2024
Decellularized extracellular matrix (dECM) offers a three-dimensional, non-immunogenic scaffold, enriched with bioactive components, making it suitable candidate for tissue regeneration. Although dECM-based scaffolds have been successfully implemented in preclinical and clinical settings within engineering regenerative medicine, the mechanisms of remodeling functional restoration are not fully understood. This review critically assesses state-of-the-art dECM scaffolds, including decellularization techniques various tissues, quality control cross-linking. It highlights properties components their latest applications multiorgan biomedicine. Additionally, addresses current challenges limitations decellularized perspectives on future directions field.
Language: Английский
Citations
25
Materials & Design, Journal Year: 2024, Volume and Issue: 241, P. 112886 - 112886
Published: March 28, 2024
The rising incidence of defects in oral and maxillofacial tissues, linked to factors such as trauma, tumors, periodontal disease, aging, poses significant challenges. Current treatments, involving autografts, allografts, synthetic graft materials, face obstacles like secondary inflammation, inadequate biocompatibility. Tissue engineering, integrating cell biology material science since the 1990s, relies heavily on biomaterial scaffolds promote adhesion, proliferation, differentiation. Traditional scaffold fabrication, including 3D printing, methods lack precision, hindering effective tissue repair by controlling distribution extracellular matrix. Biomedical engineering advancements have introduced bioprinting an innovative solution, overcoming constraints conventional scaffolds. technology enables rapid precise reconstruction damaged tissues with loaded cells, mimicking vivo environments. This paper explores key technologies inkjet-based, extrusion-based, fused deposition modeling, laser-assisted, VAT photopolymerization, freeform reversible embedding suspended hydrogels, sacrificial template printing. selection materials suitable mechanical biological properties is crucial, considering distinct requirements each technique. review provides a comprehensive survey research progress printing applications craniofacial dental serving valuable reference for future medical research.
Language: Английский
Citations
24
NPG Asia Materials, Journal Year: 2024, Volume and Issue: 16(1)
Published: Jan. 19, 2024
Abstract As one of the most intractable neurological diseases, spinal cord injury (SCI) often leads to permanent impairment in patients. Unfortunately, due complex pathological mechanisms and unique postinjury microenvironment, there is currently no way completely repair injured cord. In recent years, with rapid development tissue engineering technology, combination biomaterials medicine has provided a new idea for treating SCI. Here, we systematically summarize representative biomaterials, including natural, synthetic, nano, hybrid materials, their applications SCI treatment. addition, describe several state-of-the-art fabrication techniques engineering. Importantly, provide novel insights use biomaterial-based therapeutic strategies reduce secondary damage promote repair. Finally, discuss biomaterial clinical studies. This review aims reference future exploration regeneration strategies.
Language: Английский
Citations
23
Cell stem cell, Journal Year: 2024, Volume and Issue: 31(5), P. 772 - 787.e11
Published: April 1, 2024
Language: Английский
Citations
22
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(18)
Published: April 9, 2024
Spinal cord injury (SCI) often leads to a severe permanent disability. A poor inflammatory microenvironment and nerve electric signal conduction block are the main reasons for difficulty in spinal regeneration. In this study, black phosphorus (BP) glycyrrhizic acid (GA) integrated into methacrylate-modified silk fibroin (SF) construct bifunctional injectable hydrogel (SF/BP/GA) with appropriate conductivity ability inhibit inflammation promote neuronal regeneration after SCI. This work discovers that SF/BP/GA can reduce oxidative damage mediated by oxygen free radicals, polarization of macrophages toward anti-inflammatory M2 phenotype, expression factors, improve microenvironment. Moreover, it induces neural stem cell (NSC) differentiation neurosphere formation, restores at SCI site vivo, ameliorates motor function mice hemisection, revealing significant repair effect. An injectable, electroconductive, free-radical-scavenging is promising therapeutic strategy repair.
Language: Английский
Citations
19
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: March 27, 2024
Abstract Musculoskeletal disorders are the leading causes of physical disabilities worldwide. The poor self-repair capacity musculoskeletal tissues and absence effective therapies have driven development novel bioengineering-based therapeutic approaches. Adipose-derived stem cell (ADSC)-based being explored as new regenerative strategies for repair regeneration bone, cartilage, tendon owing to accessibility, multipotency, active paracrine activity ADSCs. In this review, recent advances in ADSCs their optimization strategies, including ADSC-derived exosomes (ADSC-Exos), biomaterials, genetic modifications, summarized. Furthermore, preclinical clinical applications ADSC-Exos, either alone or combination with growth factors biomaterials genetically modified forms, reviewed. ADSC-based hold promise management multiple types injuries. timely summary highlights provided here could offer guidance further investigations accelerate application regeneration. Graphical abstract
Language: Английский
Citations
18
Giant, Journal Year: 2024, Volume and Issue: 19, P. 100323 - 100323
Published: July 10, 2024
Inspired by the extracellular matrix (ECM), biomaterials have emerged as promising strategies in biomedical research and engineering domain, offering unique characteristics for tissue regeneration, drug delivery, therapeutic interventions, cellular investigations. The ECM, a dynamic network structure secreted various cells, primarily comprises diverse proteins capable of facilitating tissue-ECM signaling regulatory functions through its rich array bioactive substances multi-level structural properties. Drawing inspiration from intricate biochemical composition natural researchers developed to encapsulate these features create biomimetic microenvironments, such electrospinning, hydrogels/hydrogel microspheres, decellularized ECM(dECM), ECM-mimicking peptides. Furthermore, mimicking ECM components, ECM-inspired exhibit varying degrees functionalization, including providing support, cell adhesion, signal transduction, mitigating immune responses, remodeling. In summary, advancements offer significant promise addressing key challenges fields engineering, regenerative medicine, delivery.
Language: Английский
Citations
18
Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)
Published: Jan. 24, 2025
Abstract Background Interfacial heterogeneity is widely explored to reveal molecular mechanisms of force-mediated pathways due biased tension. However, the influence cell density,, curvature, and interfacial on underlying mechanotransduction obscure. Methods Polydimethylsiloxane (PDMS)-based stencils were micropatterned prepare micropores for culture. The colonies human mesenchymal stem cells (hMSCs) formed by controlling seeding density investigate influences density, curvature mechanotransduction. Immunofluorescent staining integrin, vinculin, talin-1 was conducted evaluate adhesion-related expression levels. Then, immunofluorescent actin, actinin, myosin performed detect cytoskeleton distribution, especially at periphery. Nuclear force-sensing explained yes-associated protein (YAP) laminA/C analysis. Results colony hMSCs demonstrated coincident characters with engineered microstencils. obviously developed heterogeneous morphogenesis. Heterogeneous focal adhesion guided development together regulate cellular contractility movement talin-1. Cytoskeletal showed that fibers reorganized periphery YAP nuclear translocation remodeling enhanced regulation (FA) arrangement. Conclusions clustering similar results prepared microstencils, also well adjusted establish balance colony. mechanism spreading, elongation investigated disclose compliance FA along microarrays increased may provide helpful information understanding cells.
Language: Английский
Citations
4