Circular RNA CircFOXO3 Functions as a Competitive Endogenous RNA for Acid-Sensing Ion Channel Subunit 1 Mediating Oxeiptosis in Nucleus Pulposus

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 678 - 678

Published: March 18, 2024

Oxeiptosis is a reactive oxygen species (ROS)-induced pathway of cell death. The involvement circular RNAs (circRNAs) has been confirmed in the incidence and progression intervertebral disc degeneration (IVDD). However, whether oxeiptosis occurs IVDD how circRNAs regulate still unclear. In this study, we discovered that could be induced nucleus pulposus cells (NPCs), circFOXO3 was significantly upregulated after induction. Transfection using small interfering RNA (siRNA) inhibited NPCs. Mechanistically, acid-sensing ion channel subunit 1 (ASIC1) expression by functioning as molecular sponge for miR-185-3p miR-939-5p. Subsequent rescue experiments validated NPCs via miR-185-3p/miR-939-5p-ASIC1 axis. Further research on ASIC1 functions indicated regulation achieved affecting Calcium (Ca2+) influx mediated ASIC1. A mouse model established, silencing vivo found to inhibit development activation oxeiptosis-related pathway. Overall, one factors contributing mediating oxeiptosis.

Language: Английский

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Impact of indoor air pollution on DNA damage and chromosome stability: a systematic review

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(9), P. 2817 - 2841

Published: May 28, 2024

Language: Английский

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A Compressive Review on Source, Toxicity and Biological Activity of Flavonoid

Current Topics in Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 24(24), P. 2093 - 2116

Published: Aug. 7, 2024

Flavonoids are biologically active chemicals in various fruits, plants, vegetables, and leaves, which have promising uses medicinal science. The health properties of these natural widely accepted, efforts underway to extract the specific components referred as flavonoids. demonstrate a diverse range bio-activities, anticancer, antioxidant activity, anti-cholinesterase antiinflammatory antimalarial antidiabetic neurodegenerative disease, cardiovascular effect, hepatoprotective effects, antiviral antimicrobial activity. This study aims examine prevailing trends flavonoid investigation studies, elucidate activity flavonoids, their functions uses, assess potential flavonoids preventive medications for chronic diseases, outline future research opportunities this field. review explores preventing managing diseases.

Language: Английский

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Antioxidant Functions of Vitamin D and CYP11A1-Derived Vitamin D, Tachysterol, and Lumisterol Metabolites: Mechanisms, Clinical Implications, and Future Directions

Antioxidants, Journal Year: 2024, Volume and Issue: 13(8), P. 996 - 996

Published: Aug. 17, 2024

Evidence is increasing that vitamin D and CYP11A1-derived D, tachysterol, lumisterol metabolites play a significant antioxidant role beyond its classical functions in bone health calcium metabolism. Several recent studies have linked these elements to reduced oxidative stress as well improved immune, cardiovascular, neurological result of chronic kidney disease cancer. Additionally, supplementation with this has been shown be one the most cost-effective micronutrient interventions worldwide, highlighting potential therapeutic approach. The underlying mechanisms implications function or are not understood. This comprehensive narrative review aimed at summarizing current evidence regarding molecular implicated provide general overview identify key research areas for future, offering an extensive perspective can guide both researchers clinicians management diseases associated and/or insufficient status.

Language: Английский

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Unravelling the antioxidant behaviour of self-assembly β-Sheet in silk fibroin

Redox Biology, Journal Year: 2024, Volume and Issue: 76, P. 103307 - 103307

Published: Aug. 23, 2024

Local oxidative stress in diseases or injury severely hinders cell homeostasis and organ regeneration. Antioxidant therapy is an effective strategy for treatment. Biomaterials with good biocompatibility reactive oxygen species (ROS) scavenging ability are choices antioxidant therapeutics. However, there few natural biomaterials that identified both strong activity. Here, we show, the first time, silk fibroin (SF) a antioxidant, which can eliminate ROS cells zebrafish. We further demonstrate β-sheet structures turn into random coiled structure when SF treated hydrogen peroxide. The content of be increased by heating, thus enhancing antioxidation properties SF. Therefore, serve as biomaterial therapeutics, its structure-based mechanism provides novel theoretical basis, could new cue more discovery identification.

Language: Английский

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The RNA Demethyltransferase FTO Regulates Ferroptosis in Major Depressive Disorder

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1075 - 1075

Published: Jan. 26, 2025

Major depressive disorder (MDD) is a widespread and severe mental health condition characterized by persistent low mood loss of interest. Emerging evidence suggests that ferroptosis, an iron-dependent form cell death, epigenetic dysregulation contribute to the pathogenesis MDD. This study investigates role RNA demethylase FTO autophagy regulator BECN1 in ferroptosis their regulation active compound ginsenoside Rb1 (GRb1) as potential antidepressant strategy. Hippocampal tissues from postmortem MDD patient brains mice with chronic restraint stress (CRS)-induced depression were analyzed. Ferroptosis was evaluated analyzing levels markers such glutathione (GSH) malondialdehyde (MDA). GRb1 administered CRS model gavage explore its effects on ferroptosis-related pathways. The results showed expression reduced hippocampal patients mice, promoting via disruption antioxidant system. Moreover, treatment increased expression, modulated m6A methylation, restored balance, inhibited mice. These findings reveal novel mechanism highlight promising agent for treating through targeting

Language: Английский

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Evaluation of leucomethylene blue as a probe for assessing antioxidant activity reveals a potential application in the assessment of male fertility

Advances in Redox Research, Journal Year: 2025, Volume and Issue: 14, P. 100121 - 100121

Published: Feb. 14, 2025

Language: Английский

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Mechanisms of Endometrioma-Mediated Ovarian Damage: Myths and Facts

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(7), P. 2147 - 2147

Published: March 21, 2025

Ovarian endometriomas (OEMs), cystic formations within the ovaries, are a significant manifestation of endometriosis and present in 20-40% affected women. Despite extensive research, pathogenesis remains unclear, with retrograde menstruation, coelomic metaplasia, lymphatic dissemination being proposed mechanisms. OEMs negatively impact ovarian function by reducing reserve, disrupting folliculogenesis, altering microenvironment through oxidative stress, inflammation, fibrosis. Elevated reactive oxygen species (ROS) accelerate follicular atresia, extracellular matrix remodeling contributes to damage, while immune dysregulation cytokine imbalances further exacerbate condition. The presence does not significantly affect live birth rates vitro fertilization (IVF) treatments, despite potential reductions quality quantity oocytes. However, their surgical excision compromises reserve. This review highlights complex mechanisms which impair emphasizes need for research develop strategies that mitigate these effects, ultimately improving reproductive outcomes women endometriomas.

Language: Английский

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N. sphaeroides phycocyanin subunit Ns-α and Ns-β improve C. elegans antioxidative capacity via ROS-related regulation

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18917 - e18917

Published: Feb. 10, 2025

Oxidative stress and damage to macromolecules due free radicals such as reactive oxygen species (ROS) are commonly considered factors that can impair health. This study investigated the potential antioxidant properties of two subunit proteins associated with pigment-protein complex phycocyanin derived from Nostoc sphaeroides (Gexianmi). Bacterial expression vectors were separately constructed induce engineering proteins, Ns-α Ns-β. These then examined for their enhance antioxidative capacity in Caenorhabditis elegans . Firstly, a proper concentration Ns-β vitro exhibited 2, 2-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity. Secondly, while there no other observed effects on nematodes, those treated showed significant improvements motility reduced levels lipofuscin compared control group. Furthermore, thirdly, nematodes demonstrated increased resistance oxidation, evidenced by higher survivals under oxidative conditions induced 5 mM H 2 O Notably, decline endogenous ROS levels, redox-related genes, SOD-3 CAT-1 , down-regulated following consumption proteins. Taken together, these findings suggest improve C. modulating ROS-related regulation, making them modulators responding stressors.

Language: Английский

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Cullin3 mitigates NEFA-induced oxidative stress in mammary epithelial cells: involvement of BCL2/BECN1 and autophagy

Journal of Dairy Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

High nonesterified fatty acid (NEFA) concentrations in cows with clinical ketosis lead to metabolic dysfunction mammary cells, resulting oxidative stress. Studies have shown that autophagy is impaired the glands of ketotic cows, while enhancing mitigate stress these animals. Cullin3 (CUL3), an E3 ubiquitin ligase, integral for maintaining cellular homeostasis, particularly regulation and autophagy. Whether CUL3 involved mitigating NEFA-induced unknown. This study aimed investigate protective effects underlying mechanisms whereby mitigates epithelial cells. First, gland tissue blood samples were collected from healthy [n = 12, β-hydroxybutyrate (BHB) < 0.6 mM] (n BHB > 3.0 mM). Compared had reduced productive performance, decreased expression, autophagic activity, increased status tissue. In vitro, incubating MAC-T 1.2 mM NEFA downregulated autophagy, Adenovirus-mediated overexpression attenuated accumulation peroxides reactive oxygen species (ROS), silencing via siRNA exacerbated effects. Even when nuclear factor erythroid 2 related (NFE2L2) expression was by CUL3, there no worsening reductions mRNA levels NFE2L2 downstream target genes [NADPH quinone oxidoreductase 1 (NQO1), Heme oxygenase-1 (HMOX1), Glutamate-cysteine ligase catalytic (GCLC) Glutamate cysteine modifier subunit (GCLM)]. The reduction diminished upon 5 (ATG5) suggesting alleviates Additionally, aggravated decrease BCL2 apoptosis regulator (BCL2) alleviating beclin1(BECN1) expression. Under treatment, partly mitigated CUL3-induced elevation BECN1. Overall, characterize ketosis. activation, likely through BCL2-BECN1 pathway, enhances Thus, targeting CUL3-mediated could be a promising therapeutic strategy reduce stress-induced damage bovine

Language: Английский

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