Microscopy Research and Technique,
Journal Year:
2022,
Volume and Issue:
85(6), P. 2026 - 2044
Published: Jan. 24, 2022
Herbal
medicines
are
gaining
popularity
worldwide
for
human
healthcare
because
of
their
therapeutic
potential.
However,
adulteration
and
use
unauthentic
raw
herbals
as
substitutes
have
become
a
major
issue
the
local
communities
industry
reasons
safety
efficacy.
Therefore,
authentication
medicinal
plants
before
in
herbal
is
need
time.
Hence,
present
study
was
designed
with
an
aim,
to
authenticate
Lamiaceous
taxa
by
using
pollen
traits
observed
under
scanning
electron
microscopy.
Pollen
micro-morphological
studies
solve
problem
through
discrimination
correct
identification
Lamiaceae
species
from
adulterants.
Based
on
features,
were
further
divided
into
two
sub-families
Lamioideae
(tricolpate)
Nepetoideae
(hexa-colpate).
The
grains
found
small
medium-sized,
tricolpate,
radially/bilateral
symmetrical,
sub-spheroidal
oblate
shape.
Besides,
exine
patterns
bireticulate,
reticulate
micro-reticulate,
colpus
surface
sculpturing
psilate,
gemmate,
scabrate,
verrucate
also
been
reported.
A
significant
variation
pattern
reticulum,
thickness,
number
secondary
lumina
per
primary
lumen.
Similarly,
has
hexa
zono-colpate
but
tri
tetra
observed.
this
contributes
medicinally
important
microscopic
techniques
can
help
problem.
Highlights
Authentication
Lamiceous
carried
out
techniques.
Chemotaxonomic
characterization
used
accurate
taxa.
medicinal,
palynological
phytochemical
significance
evaluated.
seen
that
determination
species.
chemotaxonomic
characterization,
our
reason
Microscopy Research and Technique,
Journal Year:
2019,
Volume and Issue:
83(1), P. 72 - 80
Published: Oct. 16, 2019
Nanoparticles
as
biomedicine
has
made
a
crucial
role
in
health
biotechnology.
Different
transition
metals
various
forms
playing
nanotechnological
advances
and
biological
applications.
Silver
one
of
the
nontoxic,
safe
inorganic
antibacterial
agents
can
serve
replacement
antibiotics.
Present
research
is
based
on
biogenic
synthesis
silver
nanoparticles
(Ag-NPs)
potential
antibiotics
from
fungal
metabolites
Penicillium
oxalicum.
We
used
different
analytical
techniques
X-ray
diffraction
(XRD)
scanning
electron
microscopy
(SEM)
for
characterization
biosynthesized
nanoparticles.
Furthermore,
activity
was
checked
against
Staphylococcus
aureus,
S.
dysenteriae,
Salmonella
typhi
by
using
well
diffusion
method
UV
visible
spectrophotometer.
Maximum
zone
inhibition
recorded
Shigella
dysenteriae
17.5
±
0.5
mm
(mm)
both
species
18.3
0.60
typhi.
The
P.
oxalicum
showed
excellent
activity.
It
concluded
our
results
that
have
significant
might
be
useful
wide
range
applications
such
bactericidal
agent
resistant
bacteria,
preventing
infections,
healing
wounds,
anti-inflammation.
Plants,
Journal Year:
2020,
Volume and Issue:
9(6), P. 691 - 691
Published: May 28, 2020
Edible
flowers
are
consumed
for
their
appearance,
colours,
nutritional
and
healthy
properties,
but
the
use
is
limited
by
actual
number
of
species.
Seven
edible
Lamiaceae
family
(Ocimeae
Mentheae
tribes)
were
investigated:
Monarda
didyma
'Fireball',
Nepeta
×
faassenii
'Six
Hills
Giant',
Ocimum
basilicum
'Blue
Spice',
O.
'Cinnamon',
citriodorum,
Salvia
discolor,
microphylla
'Hot
Lips'.
Total
soluble
sugars,
proteins,
polyphenols,
carotenoids,
ascorbic
acid
antioxidant
activity
detected.
The
species
tribe
contained
higher
sugar
content
than
Ocimeae
flowers,
opposite
with
regard
to
protein
content.
showed
high
polyphenols
carotenoids
together
two
specie
an
aroma
profile
dominated
sesquiterpene
hydrocarbons
(58.0%
in
S.
discolor
77.9%
citriodorum).
Oxygenated
monoterpenes
prevailed
Monarda,
also
present
essential
oil
this
latter
(84.5%).
By
contrast,
evidenced
non-terpenes
as
principal
class
(41.2%
77.5%,
respectively),
while
oxygenated
was
main
one
microphylla.
varieties
spp.
volatiles.
Microscopy Research and Technique,
Journal Year:
2019,
Volume and Issue:
82(9), P. 1507 - 1528
Published: June 7, 2019
Foliar
micromorphological
features
are
useful
to
elucidate
the
taxonomy
and
systematics
of
Lamiaceae
species.
Leaf
epidermal
morphology
using
scanning
electron
microscopy
light
22
species
from
15
genera
have
been
investigated
with
an
aim
solve
its
taxonomic
problem
in
correct
identification.
Various
foliar
were
observed
explain
their
importance
resolving
identification
taxa.
Two
main
types
trichomes
observed;
glandular
(GTs)
nonglandular
(NGTs).
GTs
further
divided
into
seven
subtypes
including
capitate,
subsessile
sessile
sunken,
barrel,
peltate,
clavate.
Similarly,
NGTs
also
simple
unicellular
multicellular
conical,
falcate,
cylindrical,
dendrite,
papillose,
short
hook
shape.
Quantitative
measurement
includes
length
width
trichomes,
stomatal
complex,
cells,
stomata,
index.
Based
on
characters,
a
key
was
developed
delimit
correctly
identify
studied
Further
molecular,
other
anatomical
phylogenetic
studies
recommended
strengthen
Lamiaceae.
Microscopy Research and Technique,
Journal Year:
2019,
Volume and Issue:
83(1), P. 81 - 87
Published: Oct. 18, 2019
Abstract
In
all
over
the
world,
herbal
drugs
are
usually
adulterated
with
similar
species
or
varieties
due
to
incorrect
identification.
Most
of
products
devoid
purity
and
quality,
therefore
an
attempt
was
carried
out
identify
plant
authenticate
its
drug
from
Mentha
suaveolens
.
Microscopy
tools
provide
excellent
platform
plants
at
level.
this
study,
microscopic
pharmacokinetic
parameters
M.
were
observed.
Plant
collected
high
diverse
areas
Northern
Pakistan.
Macro
micro‐morphology
including
palynology
anatomical
features
analyzed
study
Species
characteristics
studied,
while
implementing
techniques
for
delimitation
identification
species.
Traditionally
used
cure
several
diseases
that
is,
digestive
disorders,
respiratory
disorders.
Micromorphology
(stem,
leaves,
flowers
structure,
length
etc.),
(shape,
size
pollen
characters
(types
stomata,
epidermal
cell
shape,
trichomes)
studied.
great
interest
significance
discuss
taxonomy
Taxonomic
studied
characterize
The
aim
present
is
observe
in
detail
taxonomic
term
morphology,
palynology,
foliar
anatomy
correct
along
their
medicinal
uses
area.
Microscopy Research and Technique,
Journal Year:
2019,
Volume and Issue:
83(4), P. 345 - 353
Published: Dec. 2, 2019
Abstract
Recent
field
exploration
and
collections
has
led
to
the
findings
of
several
new
species
in
Pakistan.
Here
we
reported
two
Ajuga
reptance
L
Sphagneticola
trilobata
(L.)
Prusk
for
first
time
Pakistan
flora
as
these
were
neither
listed
any
other
literature
nor
identified
before
These
found
a
result
taxonomic
studies
performed
year
2019
District
Rawalpindi
Islamabad,
Microscopic
techniques
used
confirmation
foliar
epidermal
pollen
micromorphological
features.
Detailed
study
(morphological,
palynological,
epidermal)
was
provided
correct
identification
delimitation
using
both
light
scanning
electron
microscopy.
Morphological
results
compared
with
Taiwan
China.
Microscopy Research and Technique,
Journal Year:
2020,
Volume and Issue:
83(11), P. 1381 - 1390
Published: July 12, 2020
Abstract
In
the
current
investigation,
16
samples
of
six
species
genus
Polygonatum
were
collected
from
different
geographical
regions
in
China.
Leaf
micromorphological
traits
studied
detail
using
scanning
electron
microscopy.
About
five‐leaf
used
for
each
regions.
A
significant
variation
was
found
foliar
epidermal
such
as
stomata
types
and
their
distribution,
cell
shape,
anticlinal
wall
pattern,
various
trichomes.
Polygonal
cells
observed
P.
cyrtonema
,
odoratum
sibiricum
filipes
rectangular
P
.
zanlanscianense
irregular
shape
Straight
pattern
most
species,
but
straight
to
little
undulate
Similarly,
that
are,
oval,
oblong,
elliptical
is,
paracytic,
anomocytic,
actinocytic
have
been
observed.
Based
on
traits,
closely
related
one
another.
On
other
hand,
involucratum
shows
a
strong
association
However,
showed
very
less
similarity
with
five
species.
Hence
this
study
adds
useful
information
may
be
helpful
further
taxonomic
classification
taxa
at
subspecies
level
correct
identification
discrimination
Saudi Journal of Biological Sciences,
Journal Year:
2019,
Volume and Issue:
27(1), P. 567 - 573
Published: Nov. 27, 2019
Camellia
sinensis
L.
has
long
been
used
as
a
therapeutic
agent
for
the
Central
nervous
system
(CNS)
due
to
presence
of
flavonoids.
The
present
study
aimed
evaluate
dose-dependent
Neuropharmacological
behavioral
potential
seed
and
leaf
extracts
on
mice.
To
differential
extract
various
doses
were
prepared
examined
in
open
field,
head
dip,
rearing,
cage
cross,
swimming
traction
tests.
One-way
ANOVA
set
at
P*
<
0.05
followed
by
POST
HOC
LSD
(P*
0.01)
was
applied
significant
difference
among
treatments.
Herein
both
showed
results
high
doses.
Interestingly
dose
effect
mice
CNS
field
dip
test,
while
revealed
stimulus
Overall
that
produced
more
stimulant
less
calmness
compared
was.
Tea
leaves
had
already
known
drugs;
current
investigation
suggests
tea
can
be
an
alternative
with
effective
action.