Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(1), P. 72 - 80

Published: Oct. 16, 2019

Nanoparticles as biomedicine has made a crucial role in health biotechnology. Different transition metals various forms playing nanotechnological advances and biological applications. Silver one of the nontoxic, safe inorganic antibacterial agents can serve replacement antibiotics. Present research is based on biogenic synthesis silver nanoparticles (Ag-NPs) potential antibiotics from fungal metabolites Penicillium oxalicum. We used different analytical techniques X-ray diffraction (XRD) scanning electron microscopy (SEM) for characterization biosynthesized nanoparticles. Furthermore, activity was checked against Staphylococcus aureus, S. dysenteriae, Salmonella typhi by using well diffusion method UV visible spectrophotometer. Maximum zone inhibition recorded Shigella dysenteriae 17.5 ± 0.5 mm (mm) both species 18.3 0.60 typhi. The P. oxalicum showed excellent activity. It concluded our results that have significant might be useful wide range applications such bactericidal agent resistant bacteria, preventing infections, healing wounds, anti-inflammation.

Language: Английский

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Evaluation of chromium phytoremediation potential of some plant species of Dir Lower, Khyber Pakhtunkhwa, Pakistan

Acta Ecologica Sinica, Journal Year: 2019, Volume and Issue: 40(2), P. 158 - 165

Published: Dec. 19, 2019

Language: Английский

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Systematics study through scanning electron microscopy; a tool for the authentication of herbal drug Mentha suaveolens Ehrh

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(1), P. 81 - 87

Published: Oct. 18, 2019

Abstract In all over the world, herbal drugs are usually adulterated with similar species or varieties due to incorrect identification. Most of products devoid purity and quality, therefore an attempt was carried out identify plant authenticate its drug from Mentha suaveolens . Microscopy tools provide excellent platform plants at level. this study, microscopic pharmacokinetic parameters M. were observed. Plant collected high diverse areas Northern Pakistan. Macro micro‐morphology including palynology anatomical features analyzed study Species characteristics studied, while implementing techniques for delimitation identification species. Traditionally used cure several diseases that is, digestive disorders, respiratory disorders. Micromorphology (stem, leaves, flowers structure, length etc.), (shape, size pollen characters (types stomata, epidermal cell shape, trichomes) studied. great interest significance discuss taxonomy Taxonomic studied characterize The aim present is observe in detail taxonomic term morphology, palynology, foliar anatomy correct along their medicinal uses area.

Language: Английский

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Taxonomic study of one generic and two new species record to the flora of Pakistan using multiple microscopic techniques

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(4), P. 345 - 353

Published: Dec. 2, 2019

Abstract Recent field exploration and collections has led to the findings of several new species in Pakistan. Here we reported two Ajuga reptance L Sphagneticola trilobata (L.) Prusk for first time Pakistan flora as these were neither listed any other literature nor identified before These found a result taxonomic studies performed year 2019 District Rawalpindi Islamabad, Microscopic techniques used confirmation foliar epidermal pollen micromorphological features. Detailed study (morphological, palynological, epidermal) was provided correct identification delimitation using both light scanning electron microscopy. Morphological results compared with Taiwan China.

Language: Английский

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Foliar epidermal micromorphology and its taxonomic significance in Polygonatum (Asparagaceae) using scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(11), P. 1381 - 1390

Published: July 12, 2020

Abstract In the current investigation, 16 samples of six species genus Polygonatum were collected from different geographical regions in China. Leaf micromorphological traits studied detail using scanning electron microscopy. About five‐leaf used for each regions. A significant variation was found foliar epidermal such as stomata types and their distribution, cell shape, anticlinal wall pattern, various trichomes. Polygonal cells observed P. cyrtonema , odoratum sibiricum filipes rectangular P . zanlanscianense irregular shape Straight pattern most species, but straight to little undulate Similarly, that are, oval, oblong, elliptical is, paracytic, anomocytic, actinocytic have been observed. Based on traits, closely related one another. On other hand, involucratum shows a strong association However, showed very less similarity with five species. Hence this study adds useful information may be helpful further taxonomic classification taxa at subspecies level correct identification discrimination

Language: Английский

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Pollen morphology of family Solanaceae and its taxonomic significance

Anais da Academia Brasileira de Ciências, Journal Year: 2020, Volume and Issue: 92(3)

Published: Jan. 1, 2020

The pollen micro-morphology of family Solanaceae from the different phytogeographical region Pakistan has been assessed. In this study, thirteen species belonging to ten genera have studied using light and scanning electron microscopy for both qualitative quantitative features. is a eurypalynous significant variation was observed in size, shape, polarity exine sculpturing. Examined plant includes, Brugmansia suaveolens, Capsicum annuum, Cestrum parqui, Datura innoxia, Solanum lycopersicum, Nicotiana plumbaginifolia, Petunia hybrida, Physalis minima, americanum, erianthum, melongena, surattense Withania somnifera. prominent type tricolporate shed as monad. High fertility reflects that taxa are well-known study area. Based on traits taxonomic key developed accurate quick identification species. Principal Component Analysis performed shows some morphological features main characters identification. Cluster separate cluster. findings highlight importance Palyno-morphological characterization Solanaceous taxa. It concluded LM SEM significantly play role correct studied.

Language: Английский

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Foliar micromorphology of selected medicinal Lamiaceae taxa and their taxonomic implication using scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(9), P. 3217 - 3236

Published: June 18, 2022

In this research, 25 medicinally used Lamiaceae species belonging to 20 genera have been studied and identified for the nine disorders. We scanning electron microscopy (SEM) qualitative quantitative morphological character identification. The micromorphological characters observed here were important distinguishing taxa. highest medicinal values reported Vitex negundo Scutellaria baicalensis all considered categories except urinary otorhinolaryngology foliar epidermal anatomical characteristics revealed that features of provide taxonomically significant accurate identification information delimitate family species. Moreover, we focused on both (epidermal cell shape, stomata type, stomatal pore subsidiary glandular trichomes, non-glandular trichome shape) as well size, trichomes size). diversity was different in most species' adaxial abaxial surfaces. species, anomocytic observed, but other types such diacytic, paracytic, tetracytic type also examined. diverse pattern suggests taxa insight evidence taxonomic observation Traditional Chinese Medicinal plants from Lamiaceae. This work sets an avenue future research exploration flora through microscopic investigations. RESEARCH HIGHLIGHTS: offers a thorough Taxonomic authentication. Qualitative characterization

Language: Английский

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Neuropharmacological potential of various morphological parts of Camellia sinensis L.

Saudi Journal of Biological Sciences, Journal Year: 2019, Volume and Issue: 27(1), P. 567 - 573

Published: Nov. 27, 2019

Camellia sinensis L. has long been used as a therapeutic agent for the Central nervous system (CNS) due to presence of flavonoids. The present study aimed evaluate dose-dependent Neuropharmacological behavioral potential seed and leaf extracts on mice. To differential extract various doses were prepared examined in open field, head dip, rearing, cage cross, swimming traction tests. One-way ANOVA set at P* < 0.05 followed by POST HOC LSD (P* 0.01) was applied significant difference among treatments. Herein both showed results high doses. Interestingly dose effect mice CNS field dip test, while revealed stimulus Overall that produced more stimulant less calmness compared was. Tea leaves had already known drugs; current investigation suggests tea can be an alternative with effective action.

Language: Английский

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Using microscopic techniques for taxonomic implications of seed and fruits of Delphinium L. (sensu lato) (Ranunculaceae)

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(2), P. 99 - 117

Published: Nov. 11, 2019

In present research, micromorphological characters of the genus Delphinium L. (sensu lato) Ranunculaceae seeds and fruits were studied using microscopic techniques. A total 37 species light (LM) scanning electron microscopy (SEM) to evaluate characteristics for taxonomic identification relationship in genus. For each taxon, 33 observed studied. All analyzed MVSP software UPGMA method by dissimilarity index (average distance). Considerable differences trichomes shape fruit seed color features. The results revealed that all taxa are separated into three clades. first clade consists D. pallidiflorum semibarbatum, which have black two types glandular crispate fruit. second speciosum, szowitsianum, dasystachyson, aquilegifolium, ursinum, C. trigonelloides, camptocarpa, had a brown seeds, villous on also been observed. remaining classified as third having various colors including yellow, black, trichome shapes included one following forms strigose, villous, less, glandular, hooked, fruits. aim study is use delimitation Delphinium.

Language: Английский

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Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(3), P. 471 - 479

Published: Sept. 21, 2020

Abstract Lamioideae comprised the second‐largest subfamily in Lamiaceae. Although considerable progress has recently been made taxonomic study of Lamioideae, remains one most poorly investigated Therefore, present was designed with aim to document pollen micromorphology some selected taxa and its significance from Pakistan. Pollen micromorphological features were observed using scanning electron microscopy. The grains are monad, tricolpate, radially/bilateral symmetrical. small medium‐sized having oblate, oblate/subspheroidal, subspheroidal shape. Exine sculpturing as reticulate, microreticulate, bireticulate. colpus surface ornamentation found verrucate, gemmate, scabrate, psilate. There a variation between species micromorphology, that is, coarseness reticulum, thickness muri comprising reticulum number secondary lumina per primary lumen. Hence, this documented morphology Pakistan strengthens identification based on characters, which helps correct identification, discrimination at generic level.

Language: Английский

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