Palynological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan, using light and scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(9), P. 1410 - 1418

Published: May 20, 2019

Abstract This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) District Rawalpindi, Pakistan. The morphology 10 species family Apocynaceae observed and documented using light microscopy scanning electron microscopy. Pollen found subspheroidal in shape most species, however peroblate noted Vinca major . Exine sculpturing patterns (psilate, rugulate, scabrate, microreticulate) were observed. result indicated that exine ornamentation taxa is systematically informative at generic levels. Most have tricolporate type but tetraporate also Trachelospermum jasminoides tetracolpate V. Minimum equatorial diameter Carissa edulis (27.13 μm) maximum (108.25 μm). Similarly, thickness Cascabela thevetia (9.5 In present findings, morphological data are compared with available other studies to evaluate taxonomic value traits by multiple microscopic techniques. Furthermore, molecular phylogenetic recommended strengthen systematics taxa.

Language: Английский

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Using microscopic techniques for taxonomic implications of seed and fruits of Delphinium L. (sensu lato) (Ranunculaceae)

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(2), P. 99 - 117

Published: Nov. 11, 2019

In present research, micromorphological characters of the genus Delphinium L. (sensu lato) Ranunculaceae seeds and fruits were studied using microscopic techniques. A total 37 species light (LM) scanning electron microscopy (SEM) to evaluate characteristics for taxonomic identification relationship in genus. For each taxon, 33 observed studied. All analyzed MVSP software UPGMA method by dissimilarity index (average distance). Considerable differences trichomes shape fruit seed color features. The results revealed that all taxa are separated into three clades. first clade consists D. pallidiflorum semibarbatum, which have black two types glandular crispate fruit. second speciosum, szowitsianum, dasystachyson, aquilegifolium, ursinum, C. trigonelloides, camptocarpa, had a brown seeds, villous on also been observed. remaining classified as third having various colors including yellow, black, trichome shapes included one following forms strigose, villous, less, glandular, hooked, fruits. aim study is use delimitation Delphinium.

Language: Английский

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Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(3), P. 471 - 479

Published: Sept. 21, 2020

Abstract Lamioideae comprised the second‐largest subfamily in Lamiaceae. Although considerable progress has recently been made taxonomic study of Lamioideae, remains one most poorly investigated Therefore, present was designed with aim to document pollen micromorphology some selected taxa and its significance from Pakistan. Pollen micromorphological features were observed using scanning electron microscopy. The grains are monad, tricolpate, radially/bilateral symmetrical. small medium‐sized having oblate, oblate/subspheroidal, subspheroidal shape. Exine sculpturing as reticulate, microreticulate, bireticulate. colpus surface ornamentation found verrucate, gemmate, scabrate, psilate. There a variation between species micromorphology, that is, coarseness reticulum, thickness muri comprising reticulum number secondary lumina per primary lumen. Hence, this documented morphology Pakistan strengthens identification based on characters, which helps correct identification, discrimination at generic level.

Language: Английский

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Morpho‐palynological and anatomical studies in desert cacti (Opuntia dillenii and Opuntia monacantha) using light and scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(8), P. 2801 - 2812

Published: April 12, 2022

Opuntia is the most diverse and widely distributed drought resistant promising genus of family Cactaceae. The cladodes were utilized to quantify chemical composition these plants helpful in lignocellulose conversion their application towards biofuel production. present study was aimed evaluate compare taxonomic relationship based on morphology, stem anatomy palynology important desert cacti including dillenii monacantha. This also evaluates potential usefulness morphological, anatomical pollen traits using light scanning electron microscopy. obtained microcharacters flowers are considered diagnostic at generic specific levels. Some distinguishing morphological features observed elliptical obovate cladodes, 1-7 spines per areole presence glochidia O. dillenii. Pollen characters studied taxa highly species epidermis has irregular wavy cells, with straight sinuate wall pattern paracytic stomata. grains appear as pantoporate prolate spheroidal having reticulate perforate-reticulate sculpturing while exine semi-tectate tectate. could be valuable elaborate correctly identification species. methods microscopic examination providing sufficient evidence about taxonomy RESEARCH HIGHLIGHTS: Description illustration Opuntia. Morpho-anatomy LM SEM. Highly variation qualitative quantitative features. Systematic significance presented.

Language: Английский

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A Review on the Ethnopharmacology and Phytochemistry of the Neotropical Sages (Salvia Subgenus Calosphace; Lamiaceae) Emphasizing Mexican Species

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: April 19, 2022

Salvia is the most diverse genus within mint family (Lamiaceae), many of its species are well-known due to their medicinal and culinary uses. Most ethnopharmacological phytochemical studies on centred from European Asian clades. However, about clade, Neotropical sages ( subgenus Calosphace ; 587 spp.), relatively scarce. This review aims compile information traditional uses, pharmacological phytochemistry properties sages. To do so, we carried out a comprehensive articles available in different online databases published past 2022 (i.e., PubMed, Scopus, Web Science, among others) summarized tables. uncover phylogenetic patterns distribution four groups metabolites (mono-, sesqui-, di-, triterpenes), generated presence-absence matrices plotted tip states over dated phylogeny . We found several involving Mexican , but only few taxa other diversity centres. The main uses ceremonial. In medicine 56 used treat diseases 17 categories according WHO, plus cultural-bound syndromes. Pharmacological reveal wide range biological (e.g., antinociceptive, anti-inflammatory, anxiolytic, cytotoxic, antidiabetic, etc.) extracts isolated compounds 38 From these species, at least 109 have been isolated, identified evaluated pharmacologically; 73 clerodanes, 21 abietanes, six flavonoids, five sesquiterpenoids, triterpenoids. characteristic diterpenes, particularly clerodanes Amarisolide A, Tilifodiolide), that almost exclusively this group. promising resource production herbal medication, corroborate attributed them Research guided by phylogenies recommended, since closely related tend share presence similar thus properties.

Language: Английский

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Pollen morphology of selected melliferous plants and its taxonomic implications using microscopy

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(7), P. 2361 - 2380

Published: March 5, 2022

This research aimed to explore the melliferous bee flora pollen from arid zones of District Attock, Islamabad Capital and Rawalpindi. Morpho-palynological features has provided key information for classification help explain taxonomy several genera species. Therefore, in this work, we examined morphological characters potentially be used species delimitation flora. A total 18 floral species, classified into 12 different families were collected, pressed, identified then acetolyized observe grains under microscopy. Bee can described by small, medium large sized morphotypes presenting five types shapes, prolate spheroidal (seven species), sub-prolate (six oblate (three (one each) was examined. The observed polar equatorial diameter found maximum Zea mays 73.5 μm Pelargonium inquinans 66.75 respectively. Pollen six namely: tricolporate, 3-zonocolporate, monoportae, tetracolporate, tetraporate mononsulcate. Whereas highest value colpi length measured Grewia tenax (24.55 μm). Exine surface nature echinate psilate scabrate. thickness noted Verbena tenuisecta (8.40 μm) minimum Citrus macrocarpa (0.4 considered difficult identify based on other traits successfully distinguished using quantitative qualitative traits, confirming importance morphology diagnose correctly honeybee RESEARCH HIGHLIGHTS: diversity among Highly variations via microscopy micromorphology. Palyno-morph taxonomic identification

Language: Английский

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Palynological characterization and taxonomic delimitation of the genus Salvia L. in Pakistan

Palynology, Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Language: Английский

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A contribution to the knowledge of wild medicinal Lamiaceae: Taxonomic, microscopic, and ecological insights from palynology

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Language: Английский

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Taxonomic importance of spore morphology in Thelypteridaceae from Northern Pakistan

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(8), P. 1326 - 1333

Published: May 14, 2019

Abstract Spore morphology of Thelypteridaceae species growing in Malakand Division, Northern Pakistan, was studied using both light microscopy and scanning electron microscopy. The taxa are Christella dentata Glaphyropteridopsis erubescens the subfamily Thelypteridoideae, Phegopteris connectilis , Pseudophegopteris pyrrhorhachis levingei Phegopteridoideae. exhibit differences spore size, exospore thickness, color, ornamentation. Spores monolete medium‐sized, shape is ellipsoidal polar equatorial views. average measurement diameter ranges from 27 μm to 31 μm, whereas direction it varied 20 40 μm. thickness 1.2 2.4 Reticulate, laevigate with microgranules, cristate, coarsely echinate surface ornamentation observed among species. Multivariate analysis including unweighted pair group method arithmetic mean principal component used for grouping discrimination genera.

Language: Английский

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Feasible regeneration and agro bacterium-mediated transformation of Brassica juncea with Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene

Saudi Journal of Biological Sciences, Journal Year: 2020, Volume and Issue: 27(5), P. 1324 - 1332

Published: Jan. 8, 2020

In the present study an effort has been made to optimize in vitro regeneration protocol for Agrobacterium-mediated transformation of Brassica juncea, because its importance as oilseed crops. The highest callus induction frequency 87% was observed on MS (Murashige and Skoog, 1962) medium supplemented with 4 µM 6-benzyladenine (BA) after four weeks culture period. Subculturing organogenic calli media a similar hormonal composition resulted shoot organogenesis six cultivation. (92%) recorded containing BA combination 1 α-naphthalene acetic acid (NAA). Further, well-developed roots were formed augmented 6 Indole (IAA) Kinetin (Kn). Cotyledon explants exploited successful B. juncea. A binary vector comprised Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene under transcriptional control glycinin promoter basta selection marker introduced into A. tumefaciens strain GV3101 via electroporation. EaDAcT is responsible unusual triacylglycerol's production where sn-3 position esterified acetate instead long-chain fatty found triacylglycerol's. (100%) transgenic shoots plus NAA presence 25 mg l-1 160 timintin. efficiency stable be approximately 7% plants. Moreover, transformed regenerated confirmed by PCR analysis using gene-specific primers.

Language: Английский

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