Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(1), P. 72 - 80

Published: Oct. 16, 2019

Nanoparticles as biomedicine has made a crucial role in health biotechnology. Different transition metals various forms playing nanotechnological advances and biological applications. Silver one of the nontoxic, safe inorganic antibacterial agents can serve replacement antibiotics. Present research is based on biogenic synthesis silver nanoparticles (Ag-NPs) potential antibiotics from fungal metabolites Penicillium oxalicum. We used different analytical techniques X-ray diffraction (XRD) scanning electron microscopy (SEM) for characterization biosynthesized nanoparticles. Furthermore, activity was checked against Staphylococcus aureus, S. dysenteriae, Salmonella typhi by using well diffusion method UV visible spectrophotometer. Maximum zone inhibition recorded Shigella dysenteriae 17.5 ± 0.5 mm (mm) both species 18.3 0.60 typhi. The P. oxalicum showed excellent activity. It concluded our results that have significant might be useful wide range applications such bactericidal agent resistant bacteria, preventing infections, healing wounds, anti-inflammation.

Language: Английский

---

Taxonomic significance of foliar epidermal morphology in Lamiaceae from Pakistan

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(9), P. 1507 - 1528

Published: June 7, 2019

Foliar micromorphological features are useful to elucidate the taxonomy and systematics of Lamiaceae species. Leaf epidermal morphology using scanning electron microscopy light 22 species from 15 genera have been investigated with an aim solve its taxonomic problem in correct identification. Various foliar were observed explain their importance resolving identification taxa. Two main types trichomes observed; glandular (GTs) nonglandular (NGTs). GTs further divided into seven subtypes including capitate, subsessile sessile sunken, barrel, peltate, clavate. Similarly, NGTs also simple unicellular multicellular conical, falcate, cylindrical, dendrite, papillose, short hook shape. Quantitative measurement includes length width trichomes, stomatal complex, cells, stomata, index. Based on characters, a key was developed delimit correctly identify studied Further molecular, other anatomical phylogenetic studies recommended strengthen Lamiaceae.

Language: Английский

---

Petiole micromorphology in Brassicaceous taxa and its potential for accurate taxonomic identification

Flora, Journal Year: 2023, Volume and Issue: 303, P. 152280 - 152280

Published: April 16, 2023

Language: Английский

---

Palynological Study of Fossil Plants from Miocene Murree Formation of Pakistan: Clues to Investigate Palaeoclimate and Palaeoenvironment

Agronomy, Journal Year: 2023, Volume and Issue: 13(1), P. 269 - 269

Published: Jan. 16, 2023

Palaeoflora in Pakistan the Miocene is characterized by its high biodiversity. The present study investigated pollen of fossil plants from Murree Formation Pakistan. Shales and mudstones were collected section located at foothills Margalla hills analyzed palynofacies palynostratigraphic analyses. In this paleopalynological Formations Pakistan, 31 samples using microscopic techniques containing 48 types 12 families. quantitative qualitative morphological features determined light scanning electron microscopy to help identify grains. Exine ornamentations spines most important diagnostic for distinguishing one grain another. maximum exine thickness was observed Ocimum basilicum 4.25 μm, whereas diameter recorded Pinus 69.5 μm. Lamiaceae, Asteraceae, Poaceae dominant results showed that preservation floral records not optimal. presented provide data on plant taxa existed (23.03–5.33 Ma). evolution phytogeographical histories can be unraveled rock sediments preserve

Language: Английский

---

Taxonomic study of one generic and two new species record to the flora of Pakistan using multiple microscopic techniques

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(4), P. 345 - 353

Published: Dec. 2, 2019

Abstract Recent field exploration and collections has led to the findings of several new species in Pakistan. Here we reported two Ajuga reptance L Sphagneticola trilobata (L.) Prusk for first time Pakistan flora as these were neither listed any other literature nor identified before These found a result taxonomic studies performed year 2019 District Rawalpindi Islamabad, Microscopic techniques used confirmation foliar epidermal pollen micromorphological features. Detailed study (morphological, palynological, epidermal) was provided correct identification delimitation using both light scanning electron microscopy. Morphological results compared with Taiwan China.

Language: Английский

---

Macro-Morphological and Ecological Variation in Rosa sericea Complex

Agronomy, Journal Year: 2022, Volume and Issue: 12(5), P. 1078 - 1078

Published: April 29, 2022

Taxon delimitation and identification are important in many areas of biology, especially taxonomy conservation. Taxonomic treatment is required to establish justify recommendations conservation biology for the group being considered. Imperfect controversial can undermine assessment. We studied 71 populations; 665 individuals corresponding morphology Rosa sericea complex (including six taxa, one which has extremely narrow distributions) were collected from sympatric, parapatric, allopatric populations distributed China. This study aims investigate whether species macromorphologically different evaluate rare taxa priority. The morphological characters principal component analysis (PCA) R. showed that have overlapping but distinguish morphologically. systematics status based on previous DNA sequencing controversial. ecological habitat’s current only delimit morrisonensis (in Taiwan). To evade mistakes conservation, we recommend taxonomical knowledge be needed ensure success protecting target species. Thus, complementarity systemic assurance makes actions more necessary complex’s taxa. niche modelling (ENM) results habitats these conspecific would shrunken. With presence snapshots time, geography might decrease rapidly representative entirely Geographic space (G-space) Environmental (E-space) such bright inhabit. So far, significant inferences meant occupy most incredible comparative research, taking impermanent nature distributions undertaking at a state stability. If artificially identified (rarely distributed) identification, they must conserved.

Language: Английский

---

Foliar micromorphology of selected medicinal Lamiaceae taxa and their taxonomic implication using scanning electron microscopy

Microscopy Research and Technique, Journal Year: 2022, Volume and Issue: 85(9), P. 3217 - 3236

Published: June 18, 2022

In this research, 25 medicinally used Lamiaceae species belonging to 20 genera have been studied and identified for the nine disorders. We scanning electron microscopy (SEM) qualitative quantitative morphological character identification. The micromorphological characters observed here were important distinguishing taxa. highest medicinal values reported Vitex negundo Scutellaria baicalensis all considered categories except urinary otorhinolaryngology foliar epidermal anatomical characteristics revealed that features of provide taxonomically significant accurate identification information delimitate family species. Moreover, we focused on both (epidermal cell shape, stomata type, stomatal pore subsidiary glandular trichomes, non-glandular trichome shape) as well size, trichomes size). diversity was different in most species' adaxial abaxial surfaces. species, anomocytic observed, but other types such diacytic, paracytic, tetracytic type also examined. diverse pattern suggests taxa insight evidence taxonomic observation Traditional Chinese Medicinal plants from Lamiaceae. This work sets an avenue future research exploration flora through microscopic investigations. RESEARCH HIGHLIGHTS: offers a thorough Taxonomic authentication. Qualitative characterization

Language: Английский

---

Pollen Morphology of Rosa sericea Complex and Their Taxonomic Contribution

Diversity, Journal Year: 2022, Volume and Issue: 14(9), P. 705 - 705

Published: Aug. 26, 2022

In this research, the pollen morphology of Rosa sericea complex has been studied by Scanning Electron Microscope (SEM). The present study aimed to provide baseline information for taxonomic identification R. taxa. sculpture, outline, and aperture show variability in includes taxonomically challenging species with undistinguishable boundaries. Identifying taxon boundaries closely related is fundamental investigating evolution biodiversity. Taxa circumscription insufficient, unresolved, a prerequisite comprehensive systematic revisions. To resolve these issues, we have attempted identify some important diagnostic characters complex. This research different qualitative quantitative characteristics pollen. included polar equatorial view, apertures, exine, size category, polarity contrast, features include diameter, P/E, colpi length width, distance between colpi. Pollen mono tricolporate. morphological here very few details which separate into distinct Some offer delimitation including apertures.

Language: Английский

---

Using microscopic techniques for taxonomic implications of seed and fruits of Delphinium L. (sensu lato) (Ranunculaceae)

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(2), P. 99 - 117

Published: Nov. 11, 2019

In present research, micromorphological characters of the genus Delphinium L. (sensu lato) Ranunculaceae seeds and fruits were studied using microscopic techniques. A total 37 species light (LM) scanning electron microscopy (SEM) to evaluate characteristics for taxonomic identification relationship in genus. For each taxon, 33 observed studied. All analyzed MVSP software UPGMA method by dissimilarity index (average distance). Considerable differences trichomes shape fruit seed color features. The results revealed that all taxa are separated into three clades. first clade consists D. pallidiflorum semibarbatum, which have black two types glandular crispate fruit. second speciosum, szowitsianum, dasystachyson, aquilegifolium, ursinum, C. trigonelloides, camptocarpa, had a brown seeds, villous on also been observed. remaining classified as third having various colors including yellow, black, trichome shapes included one following forms strigose, villous, less, glandular, hooked, fruits. aim study is use delimitation Delphinium.

Language: Английский

---

Palynological Study of Weed Flora from Potohar Plateau

Agronomy, Journal Year: 2022, Volume and Issue: 12(10), P. 2500 - 2500

Published: Oct. 13, 2022

The pollen morphology of weeds was investigated by scanning electron microscopy (SEM). A morpho-palynological investigation 18 species that belongs to 16 angiosperms families performed using SEM document distinguishable microscopic features. main objective the present study provide basic knowledge about features weed helps delimit flora Potohar Plateau. results show diversity among qualitative and quantitative characteristics shape, equatorial polar axis diameter, exine’s thickness, surface ornamentation. grains were spherical, prolate-spheroidal, oblate-spheroidal, sub-oblate. exine ornamentation in most reticulate, scarbate, aerolate, faveolate, reticulate-perforate, reticulate-scabrate. All described possessed tricolpate pollen. variations found thickness other characters helpful at genus species-specific levels. In accordance with these variations, a taxonomic key prepared identify differentiate plant species. images can help taxa level. This provides baseline information distinguish weeds.

Language: Английский

---