miR-142-3p encapsulated in T lymphocyte-derived tissue small extracellular vesicles induces Treg function defect and thyrocyte destruction in Hashimoto’s thyroiditis DOI Creative Commons
Genpeng Li, Linye He, Jing Huang

et al.

BMC Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: June 6, 2023

Hashimoto's thyroiditis (HT) is an organ-specific autoimmune disease characterized by lymphocyte infiltration that destroys thyrocyte cells. The aim of the present study was to elucidate role and mechanisms tissue small extracellular vesicle (sEV) microRNAs (miRNAs) in pathogenesis HT.Differentially expressed sEV miRNAs were identified between HT normal RNA sequencing testing set (n = 20). Subsequently, using quantitative real-time polymerase chain reaction (qRT‒PCR) assays logistic regression analysis validation 60), most relevant verified. parental recipient cells miRNA then explored. In vitro vivo experiments further performed function potential contribute development HT.We miR-142-3p encapsulated T lymphocyte-derived sEVs can induce Treg defect destruction through intact response loop. Inactivation effectively protect non-obese diabetic (NOD).H-2h4 mice from display reduced infiltration, lower antibody titers, higher Looking at underlying action on destruction, we found strong deleterious effect mediated due its ability block activation ERK1/2 signaling pathway downregulating RAC1.Our findings highlight fact sEV-mediated transfer serve as a communication mode lymphocytes HT, favoring progression HT.

Language: Английский

Tissue‐derived extracellular vesicles in cancers and non‐cancer diseases: Present and future DOI

Su‐Ran Li,

Qi‐Wen Man, Xin Gao

et al.

Journal of Extracellular Vesicles, Journal Year: 2021, Volume and Issue: 10(14)

Published: Dec. 1, 2021

Extracellular vesicles (EVs) are lipid-bilayer membrane structures secreted by most cell types. EVs act as messengers via the horizontal transfer of lipids, proteins, and nucleic acids, influence various pathophysiological processes in both parent recipient cells. Compared to obtained from body fluids or culture supernatants, isolated directly tissues possess a number advantages, including tissue specificity, accurate reflection microenvironment, etc., thus, attention should be paid tissue-derived (Ti-EVs). Ti-EVs present interstitium play pivotal roles intercellular communication. Moreover, provide an excellent snapshot interactions among types with common histological background. Thus, may used gain insights into development progression diseases. To date, extensive investigations have focused on role fluid-derived culture-derived EVs; however, studies remains insufficient. Herein, we summarize latest advances for cancers non-cancer We propose future application basic research clinical practice. Workflows Ti-EV isolation characterization between diseases reviewed compared. discuss current issues associated potential directions.

Language: Английский

Citations

142
Liquid biopsy techniques and pancreatic cancer: diagnosis, monitoring, and evaluation DOI Creative Commons
Kangchun Wang, Xin Wang, Qi Pan

et al.

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Oct. 6, 2023

Abstract Pancreatic cancer (PC) is one of the most common malignancies. Surgical resection a potential curative approach for PC, but patients are unsuitable operations when at time diagnosis. Even with surgery, some may still experience tumour metastasis during operation or shortly after as precise prognosis evaluation not always possible. If miss opportunity surgery and resort to chemotherapy, they face challenging issue chemotherapy resistance. In recent years, liquid biopsy has shown promising prospects in disease diagnosis, treatment monitoring, assessment. As noninvasive detection method, offers advantages over traditional diagnostic procedures, such tissue biopsy, terms both cost-effectiveness convenience. The information provided by helps clinical practitioners understand molecular mechanisms underlying occurrence development, enabling formulation more personalized decisions each patient. This review introduces biomarkers methods including circulating cells (CTCs), DNA (ctDNA), noncoding RNAs (ncRNAs), extracellular vesicles (EVs) exosomes. Additionally, we summarize applications early response, resistance assessment, prognostic PC.

Language: Английский

Citations

44
Tumor Exosomal ENPP1 Hydrolyzes cGAMP to Inhibit cGAS‐STING Signaling DOI Creative Commons
Yu An,

Jinchao Zhu,

Qihui Xie

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)

Published: March 18, 2024

To evade immune surveillance, tumor cells express ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) on the surface of their membrane, which degrades extracellular cyclic GMP-AMP (cGAMP), thereby inhibiting synthase (cGAS) stimulator interferon gene (STING) DNA-sensing pathway. fully understand this stealth mechanism, it is essential to determine whether other forms ENPP1 with hydrolytic cGAMP activity also are present in microenvironment regulate innate Herein, reported that various tumor-derived exosomes carry ENPP1, and can hydrolyze synthetic 2'3'-cGAMP endogenous produced by inhibit cGAS-STING pathway cells. Moreover, exosomal bound LL-37 (an effective transporter 2'3'-cGAMP) STING signaling. Furthermore, high expression observed isolated from human breast lung cancer tissue, inhibited infiltration CD8+ T CD4+ The results elucidate function pathway, furthering understanding crosstalk between system.

Language: Английский

Citations

19
Recent advances in the use of extracellular vesicles from adipose-derived stem cells for regenerative medical therapeutics DOI Creative Commons

Song Yang,

Yiran Sun,

Chenchen Yan

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 6, 2024

Abstract Adipose-derived stem cells (ADSCs) are a subset of mesenchymal (MSCs) isolated from adipose tissue. They possess remarkable properties, including multipotency, self-renewal, and easy clinical availability. ADSCs also capable promoting tissue regeneration through the secretion various cytokines, factors, extracellular vesicles (EVs). ADSC-derived EVs (ADSC-EVs) act as intercellular signaling mediators that encapsulate range biomolecules. These have been found to mediate therapeutic activities donor by proliferation migration effector cells, facilitating angiogenesis, modulating immunity, performing other specific functions in different tissues. Compared themselves, ADSC-EVs offer advantages such fewer safety concerns more convenient transportation storage for application. As result, these received significant attention cell-free agents with potential future application regenerative medicine. In this review, we focus on recent research progress regarding medical use across conditions, wound healing, chronic limb ischemia, myocardial infarction, diabetic nephropathy, fat graft survival, bone regeneration, cartilage tendinopathy tendon peripheral nerve acute lung injury, among others. We discuss underlying mechanisms responsible inducing effects. believe deciphering biological effects, associated will provide foundation developing novel approach Graphical

Language: Английский

Citations

17
Application of engineered extracellular vesicles for targeted tumor therapy DOI Creative Commons
Fusheng Zhang, J.J. Guo,

Zhenghou Zhang

et al.

Journal of Biomedical Science, Journal Year: 2022, Volume and Issue: 29(1)

Published: Feb. 21, 2022

Abstract All cells, including prokaryotes and eukaryotes, could release extracellular vesicles (EVs). EVs contain many cellular components, RNA, surface proteins, are essential for maintaining normal intercellular communication homeostasis of the internal environment. released from different tissues cells exhibit excellent properties functions (e.g., targeting specificity, regulatory ability, physical durability, immunogenicity), rendering them a potential new option drug delivery precision therapy. have been demonstrated to transport antitumor drugs tumor therapy; additionally, EVs' contents substance can be altered improve their therapeutic efficacy in clinic by boosting effectiveness. regulate immune system function affecting microenvironment, thereby inhibiting progression. Co-delivery systems utilized further efficiency EVs, hydrogels liposomes. In this review, we discuss isolation technologies as well engineering approaches modification. Moreover, evaluate tumors, engineered co-delivery systems.

Language: Английский

Citations

55
Tumor-Derived Exosomes in Tumor-Induced Immune Suppression DOI Open Access
Qiongyu Hao, Yong Wu, Yanyuan Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(3), P. 1461 - 1461

Published: Jan. 27, 2022

Exosomes are a class of small membrane-bound extracellular vesicles released by almost all cell types and present in body fluids. Based on the studies exosome content their interactions with recipient cells, exosomes now thought to mediate "targeted" information transfer. Tumor-derived (TEX) carry cargo molecules different from that normal cell-derived exosomes. TEX functions distinct biological effects such as receptor discharge intercellular cross-talk. The immune system defenses, which may initially restrict tumor progression, progressively blunted broad array activate suppressive pathways cells. Herein, we provide review latest research progress context tumor-mediated suppression discuss potential well challenges target immunotherapy.

Language: Английский

Citations

48
A Fluid Multivalent Magnetic Interface for High‐Performance Isolation and Proteomic Profiling of Tumor‐Derived Extracellular Vesicles DOI
Qi Niu, Yun Shu, Yuanqiang Chen

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(21)

Published: March 24, 2023

Isolation and analysis of tumor-derived extracellular vesicles (T-EVs) are important for clinical cancer management. Here, we develop a fluid multivalent magnetic interface (FluidmagFace) in microfluidic chip high-performance isolation, release, protein profiling T-EVs. The FluidmagFace increases affinity by 105-fold with fluidity-enhanced binding to improve isolation efficiency 13.9 % compared non-fluid interface. Its anti-adsorption property hydrodynamic shear minimize contamination, increasing detection sensitivity two orders magnitude. Moreover, its reversibility expandability allow high-throughput recovery T-EVs mass spectrometric analysis. With the chip, were detected all tested samples identification differentially expressed proteins healthy controls. opens new avenue release targets diagnosis biomarker discovery.

Language: Английский

Citations

34
Beyond Extracellular Vesicles: Hybrid Membrane Nanovesicles as Emerging Advanced Tools for Biomedical Applications DOI Creative Commons
Meng Sun,

Jiani Yang,

Yueyun Fan

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(32)

Published: Sept. 25, 2023

Extracellular vesicles (EVs), involved in essential physiological and pathological processes of the organism, have emerged as powerful tools for disease treatment owing to their unique natural biological characteristics artificially acquired advantages. However, limited targeting ability, insufficient production yield, low drug-loading capability simplex EVs greatly hindered development clinical translation. Therefore, establishment multifunctional hybrid membrane nanovesicles (HMNVs) with favorable adaptability flexibility has become key expanding practical application EVs. This timely review summarizes current progress HMNVs biomedical applications. Different preparation strategies including physical, chemical, chimera approaches are first discussed. then individually describes diverse types based on homologous or heterologous cell substances, a fusion liposome, well bacterial membrane. Subsequently, specific emphasis is placed highlight applications toward various diseases representative examples. Finally, ongoing challenges prospects currently developed translational briefly presented. will not only stimulate broad interest among researchers from disciplines but also provide valuable insights promising nanoplatforms precision medicine.

Language: Английский

Citations

34
Extracellular Vesicles as New Players in Drug Delivery: A Focus on Red Blood Cells-Derived EVs DOI Creative Commons
Sara Biagiotti,

Faiza Abbas,

Mariele Montanari

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(2), P. 365 - 365

Published: Jan. 21, 2023

The article is divided into several sections, focusing on extracellular vesicles’ (EVs) nature, features, commonly employed methodologies and strategies for their isolation/preparation, characterization/visualization. This work aims to give an overview of advances in EVs’ extensive nanomedical-drug delivery applications. Furthermore, considerations EVs translation clinical application are summarized here, before the review a special kind vesicles, ones derived from red blood cells (RBCEVs). Generally, employing as drug carriers means managing entities with advantageous properties over synthetic vehicles or nanoparticles. Besides fact that certain also reveal intrinsic therapeutic characteristics, regenerative medicine, nanosize, lipidomic proteomic profiles enable them pass biologic barriers display cell/tissue tropisms; indeed, engineering can further optimize organ targeting. In second part review, we focus our attention RBCEVs. First, describe biogenesis composition those naturally produced by (RBCs) under physiological pathological conditions. Afterwards, discuss current procedures isolate and/or produce RBCEVs lab load specific cargo exploitation. Finally, disclose most recent applications at vitro preclinical research level potential industrial conclusion, be, near future, very promising versatile platform pharmaceutical exploitations.

Language: Английский

Citations

26
Neonatal‐Tissue‐Derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine DOI

Peng Lou,

Shuyun Liu, Yizhuo Wang

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(33)

Published: May 6, 2023

Extracellular vesicle (EV)-based therapies have emerged as a promising means in regenerative medicine. However, the conventional EV therapy strategy displays some limitations, such inefficient production and lack of tissue-specific repair effects. Here, it is reported that neonatal-tissue-derived (NEXT) potent for precision tissue repair. In brief, large amounts EVs with higher yield/purity can be readily isolated from desired tissues less time/cost compared to cell-culture-based method. Moreover, source factors, age type, affect efficacy tissue-derived different injury models (skin wounds acute kidney injury), show superior potency adult-tissue-derived EVs. Different age- or tissue-type-derived distinct composition (e.g., protein) signatures are likely due diverse metabolic patterns donor tissues, which may contribute specific action modes NEXT types injury. Furthermore, incorporated bioactive materials advanced This study highlights provide new avenue many

Language: Английский

Citations

26