Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)
Published: June 27, 2024
Extracellular vesicles (EVs) are promising next-generation therapeutics and drug delivery systems due to demonstrated safety efficacy in preclinical models early-stage clinical trials. There is an urgent need address the immunogenicity of EVs (beyond apparent lack immunotoxicity) advance development. To date, few studies have assessed unintended immunological recognition EVs. An in-depth understanding EV-induced clearance necessary develop effective therapeutic strategies, including approaches mitigate when undesired. This article summarizes various factors involved potential strategies reduce for improved benefit.
Language: Английский
Citations
44
Circulation Research, Journal Year: 2024, Volume and Issue: 135(1), P. 198 - 221
Published: June 20, 2024
From their humble discovery as cellular debris to cementing natural capacity transfer functional molecules between cells, the long-winded journey of extracellular vesicles (EVs) now stands at precipice a next-generation cell-free therapeutic tool revolutionize modern-day medicine. This perspective provides snapshot EVs emergence vibrant field biology and renaissance they usher in biomedical sciences agents for cardiovascular pathologies. Rapid development bioengineered is providing innovative opportunities overcome biological challenges such potency, cargo loading enhanced secretion, targeting circulation half-life, localized sustained delivery strategies, approaches enhance systemic circulation, uptake lysosomal escape, logistical hurdles encompassing scalability, cost, time. A multidisciplinary collaboration beyond extends chemistry, physics, biomaterials, nanotechnology, allowing rapid designer that are entering late-stage human clinical trials.
Language: Английский
Citations
19
Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(6)
Published: June 1, 2024
Abstract Food‐derived extracellular vesicles (FEVs) are nanoscale membrane obtained from dietary materials such as breast milk, plants and probiotics. Distinct other EVs, FEVs can survive the harsh degrading conditions in gastrointestinal tract reach intestines. This unique feature allows to be promising prebiotics health oral nanomedicine for gut disorders, inflammatory bowel disease. Interestingly, therapeutic effects of have recently also been observed non‐gastrointestinal diseases. However, mechanisms remain unclear or even mysterious. It is speculated that orally administered could enter bloodstream, remote organs, thus exert therein. emerging evidence suggests amount reaching organs beyond marginal may insufficient account significant achieved regarding diseases involving liver. Thus, we herein propose primarily act locally intestine by modulating intestinal microenvironments barrier integrity microbiota, thereby eliciting impact remotely on liver via gut‐liver axis. Likewise, drugs delivered system through As main metabolic hub, microenvironment implicated In fact, many patients with non‐alcoholic fatty disease, obesity, diabetes cardiovascular disease suffer a leaky dysbiosis. this review, provide an overview recent progress discuss their biomedical applications agents drug delivery systems, highlighting pivotal role axis action treatment disorders
Language: Английский
Citations
18
Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 707 - 707
Published: May 30, 2024
Mesenchymal stem cells (MSCs) have emerged as a promising approach for drug delivery strategies because of their unique properties. These include cell membrane-coated nanoparticles, cell-derived extracellular vesicles, immunomodulatory effects, cell-laden scaffolds, and scaffold-free sheets. MSCs offer advantages such low immunogenicity, homing ability, tumor tropism, making them ideal targeted systems. Stem vesicles gained attention immune properties tumor-homing abilities, presenting potential solution challenges. The relationship between MSC-based the self-renewal differentiation capabilities lies in engineered to serve effective carriers therapeutic agents while maintaining intrinsic exhibit potent immunosuppressive functions strategies. EVs immunogenicity strong tissue repair regeneration. Scaffold-free sheets represent cutting-edge regenerative medicine, offering versatile platform engineering regeneration across different medical specialties. shown great clinical applications medicine ability differentiate into various types, secrete bioactive factors, modulate responses. Researchers are exploring these innovative approaches enhance efficiency effectiveness treating diseases.
Language: Английский
Citations
13
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)
Published: June 18, 2024
Tissue-specific delivery of oligonucleotide therapeutics beyond the liver remains a key challenge in nucleic acid drug development. To address this issue, exploiting exosomes as novel carrier has emerged promising approach for efficient delivery. However, current exosome-based systems still face multiple hurdles their clinical applications. Herein, work presents strategy constructing hybrid exosome vehicle (HEV) through DNA zipper-mediated membrane fusion tissue-specific siRNA As proof-of-concept, successfully fuses liposome encapsulating anti-NFKBIZ siRNAs with corneal epithelium cell (CEC)-derived to form HEV construct treatment dry eye disease (DED). With homing characteristics inherited from exosomes, siRNA-bearing can target its parent cells and efficiently deliver payloads cornea. Subsequently, NFKBIZ gene silencing significantly reduces pro-inflammatory cytokine secretions ocular surface, reshapes inflammatory microenvironment, ultimately achieves an excellent therapeutic outcome DED mouse model. versatile platform, targeting capability designed may hold great potential various treatments.
Language: Английский
Citations
13
Pharmacological Research, Journal Year: 2024, Volume and Issue: 206, P. 107307 - 107307
Published: July 14, 2024
Extracellular vesicles (EVs), secreted by most cells, act as natural cell-derived carriers for delivering proteins, nucleic acids, and organelles between cells. Mitochondria are highly dynamic responsible energy production cellular physiological processes. Recent evidence has highlighted the pivotal role of EVs in intercellular mitochondrial content transfer, including DNA (mtDNA), intact mitochondria. Intriguingly, mitochondria crucial mediators release, suggesting an interplay their potential implications physiology pathology. However, this expanding field, much remains unknown regarding function mechanism crosstalk transport EVs. Herein, we shed light on pathological functions mitochondria, mechanisms underlying interactions, delivery mitochondria-rich EVs, clinical applications regenerative medicine.
Language: Английский
Citations
11
Nano Letters, Journal Year: 2024, Volume and Issue: 24(18), P. 5593 - 5602
Published: April 15, 2024
The design of intracellular delivery systems for protein drugs remains a challenge due to limited efficacy and serum stability. Herein, we propose reversible assembly strategy assemble cargo proteins phenolic polymers into stable nanoparticles this purpose using heterobifunctional adaptor (2-formylbenzeneboronic acid). is easily decorated on via iminoboronate chemistry further conjugates with catechol-bearing form boronate diester linkages. exhibit excellent stability in culture media but rapidly release the triggered by lysosomal acidity GSH after endocytosis. In proof-of-concept animal model, successfully transports superoxide dismutase retina intravitreal injection efficiently ameliorates oxidative stress cellular damage induced ischemia-reperfusion (I/R) minimal adverse effects. represents robust efficient method develop serum-stable biomacromolecules.
Language: Английский
Citations
9
Methods in pharmacology and toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 125 - 153
Published: Jan. 1, 2025
Language: Английский
Citations
1
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.
Language: Английский
Citations
1
International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 3175 - 3199
Published: March 1, 2025
Extracellular vesicles (EVs) participate in intercellular communication and play an essential role physiological pathological processes. In recent years, EVs have garnered significant attention as cell-free therapeutic alternatives, vectors for drug gene delivery, biomarkers disease diagnosis prognosis, vaccine development, nutraceuticals. The biodistribution of critically influences their efficacy toxicity. Therefore, this review aims to discuss the main factors influencing unmodified EVs, highlighting distribution patterns, advantages, limitations, applications under different routes administration. addition, we provide a comprehensive discussion currently available sources summarize current status potentials EVs. By optimizing administration selecting appropriate EV sources, aim offer valuable insights enhance delivery efficiency target tissues.
Language: Английский
Citations
1