Biosafety and Health, Journal Year: 2024, Volume and Issue: 6(5), P. 260 - 269
Published: Sept. 11, 2024
Language: Английский
ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(50), P. 58079 - 58091
Published: Dec. 8, 2023
Monkeypox virus (MPXV) infection was classified as a public health emergency of international concern by the World Health Organization (WHO) in 2022, being transmitted between humans large respiratory droplets, contact with skin lesions, fomites, and sexually. Currently, there are no available accessible simple-to-use diagnostic tests that accurately detect MPXV antigens for decentralized frequent testing. Here, we report an electrochemical biosensor to saliva plasma samples within 15 min using materials. The system manufactured onto paper substrate engraved CO2 laser machine, modified gold nanostructures (AuNS) monoclonal antibody, enabling sensitive detection A29 viral protein. test is based on use impedance spectroscopy (EIS) can be run miniaturized potentiostat connected smartphone. impedimetric biosensing method presented excellent analytical parameters, glycoprotein concentration ranging from 1 × 10–14 10–7 g mL–1, limit (LOD) 3.0 10–16 mL–1. Furthermore, it enabled 10–1 104 PFU LOD 7.8 10–3 Importantly, cross-reactivity observed when our device tested presence other poxvirus nonpoxvirus strains, indicating adequate selectivity nanobiosensor detection. Collectively, presents high greenness metrics associated reproducible large-scale fabrication method, sustainable substrate, low volume sample (2.5 μL), which could facilitate testing at point-of-care (POC).
Language: Английский
Citations
23
Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125663 - 125663
Published: Jan. 16, 2024
Language: Английский
Citations
12
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(21), P. 8342 - 8348
Published: May 10, 2024
In this study, we reported a selective impedimetric biosensor for the detection of A29 which is target protein monkeypox virus (MPXV). The working principle relies on interaction mechanism between A29, an internal membrane MPXV, and heparan sulfate receptor. For purpose, after immobilizing onto gold screen-printed electrode surface, its with was monitored using electrochemical impedance spectroscopy. After optimization experimental parameters, analytical characteristics developed MPVX immunosensor were examined. exhibited linear range 2.0 50 ng mL
Language: Английский
Citations
12
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 257, P. 116268 - 116268
Published: April 15, 2024
Language: Английский
Citations
9
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117221 - 117221
Published: Jan. 30, 2025
Language: Английский
Citations
1
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11
Published: April 12, 2023
The CRISPR (Clustered Regularly Spaced Short Palindromic Repeats) system was first discovered in prokaryotes as a unique immune mechanism to clear foreign nucleic acids. It has been rapidly and extensively used basic applied research owing its strong ability of gene editing, regulation detection eukaryotes. Hererin this article, we reviewed the biology, mechanisms relevance CRISPR-Cas technology applications severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis. acid tools include CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, CRISPR-Cas14, amplification technology, colorimetric readout system. above technologies have detection, including SARS-CoV-2 detection. Common based on derivation SHERLOCK, DETECTR, STOPCovid. biosensing widely point-of-care testing (POCT) by targeting recognition both DNA molecules RNA Molecules.
Language: Английский
Citations
17
Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(1)
Published: Jan. 1, 2024
Abstract COVID‐19, caused by SARS‐CoV‐2, remains a global health crisis. The emergence of multiple variants with enhanced characteristics necessitates their detection and monitoring. Genome sequencing, the gold standard, faces implementation challenges due to complexity, cost, limited throughput. CRISPR‐Cas system offers promising potential for rapid variant detection, advantages such as speed, sensitivity, specificity, programmability. This review provides an in‐depth examination applications in mutation specifically SARS‐CoV‐2. It begins introducing SARS‐CoV‐2 existing platforms. principles are then clarified, followed exploration three CRISPR‐Cas‐based platforms, which evaluated from different perspectives. discusses strategies site selection utilization CRISPR‐Cas, offering valuable insights development methods. Furthermore, critical analysis clinical applications, advantages, disadvantages, challenges, prospects is provided.
Language: Английский
Citations
8
Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(2)
Published: Jan. 17, 2024
Language: Английский
Citations
7
iLABMED, Journal Year: 2024, Volume and Issue: 2(1), P. 6 - 13
Published: March 1, 2024
Abstract Mpox (formerly Monkeypox) is a zoonotic infection caused by Monkeypox virus (MPXV). Since 2022, epidemics have occurred in many non‐endemic countries and regions, leading the World Health Organization to declare public health emergency of international concern. With persistent transmission evolution MPXV, symptoms become milder, with some infections being asymptomatic. In addition, MPXV has more contagious. Therefore, rapid accurate diagnosis screening vital prevent control epidemics. Here, we review summarize technical details, application scenarios, advantages disadvantages MPXV‐specific diagnostic methods.
Language: Английский
Citations
7
Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 390, P. 133950 - 133950
Published: May 7, 2023
Language: Английский
Citations
16