Application of CRISPR/Cas Technology in Biological Nano‐Analysis DOI
Pingping Liu,

Gerile Aodeng,

Lu Ga

et al.

ChemistrySelect, Journal Year: 2023, Volume and Issue: 8(45)

Published: Dec. 5, 2023

Abstract Currently, with the growing global demand for infectious disease diagnostics, environmental testing and food safety, CRISPR‐Cas (Clustered Regularly Interspaced Short Palindromic Repeats‐CRISPR associated proteins) system has unlimited potential as one of emerging technologies in field biotechnology. Recent studies have shown that become a powerful weapon gene editing, treatment, molecular diagnosis imaging. As important biomarkers, various nucleic acid non‐nucleic targets need to be detected from samples before situation deteriorates. In addition, there are still some limitations challenges current biosensor platforms based on system. Therefore, summarising strategies, strengths weaknesses, development prospects target identification is beneficial fully explore directions CRISPR‐Cas‐based systems. This review briefly introduces summarises classification, mechanisms applications (diagnostics when combined signal detection means). limitations, future optimisation this discussed.

Language: Английский

Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance DOI Creative Commons
Kobra Omidfar,

Fatemeh Riahi,

Soheila Kashanian

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 837 - 837

Published: Aug. 23, 2023

Lateral flow tests are one of the most important types paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving rapid screening and management infections in global pandemics or other health disorders by using minimally expert staff locations where no sophisticated laboratory services accessible. They can detect different biomarkers various biological samples provide results a little time at low price. An challenge regarding conventional LFAs is increasing their sensitivity specificity. There two main approaches to increase specificity, including assay improvement target enrichment. Assay comprises optimization signal amplification techniques. In this study, summarize specificity enhancement strategies with objective evaluation presented, such detection element immobilization, capillary rate adjusting, label evolution, sample extraction enrichment, etc. also key findings LFA performance solving limitations discussed along numerous examples.

Language: Английский

Citations

53
CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus DOI

Xue Zhao,

Yawen He,

Shengjie Shao

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(5), P. 2413 - 2420

Published: April 18, 2024

The highly contagious nature and 100% fatality rate contribute to the ongoing expanding impact of African swine fever virus (ASFV), causing significant economic losses worldwide. Herein, we developed a cascaded colorimetric detection using combination CRISPR/Cas14a system, G-quadruplex DNAzyme, microfluidic paper-based analytical device. This CRISPR/Cas14a-G4 biosensor could detect ASFV as low 5 copies/μL differentiate wild-type mutated DNA with 2-nt difference. Moreover, this approach was employed in porcine plasma. A broad linear range observed, limit spiked plasma calculated be 42–85 copies/μL. Our results indicate that paper platform exhibits advantages high sensitivity, excellent specificity, cost, making it promising for clinical applications field disease suitable popularization low-resourced areas.

Language: Английский

Citations

22
CRISPR-Microfluidics Nexus: Advancing Biomedical Applications for Understanding and Detection DOI Creative Commons

ZahraSadat Razavi,

M. Soltani, Hamidreza Pazoki‐Toroudi

et al.

Sensors and Actuators A Physical, Journal Year: 2024, Volume and Issue: 376, P. 115625 - 115625

Published: June 24, 2024

The integration of CRISPR technology with microfluidic-based biosensors has greatly expanded its applications in medicine and molecular biology. This combination offers enhanced sensitivity selectivity medical diagnostics. CRISPR-powered microfluidics enables the accurate quantification DNA RNA associated diseases such as cancer, viral infections, bacterial diseases. precise targeting capabilities allow for detection specific genetic sequences, aiding early diagnosis, disease monitoring, treatment assessment. improves specificity by leveraging programmable nature coupling it signal readouts. However, challenges assay optimization, standardization, device fabrication need to be addressed. Additionally, complexity presents potential limitations terms off-target effects unintended modifications. Overall, holds tremendous diagnostics, but further research development are required fully exploit benefits clinical settings.

Language: Английский

Citations

20
Progress and prospect: Biosynthesis of plant natural products based on plant chassis DOI

Junjie Lin,

Xue Yin,

Youran Zeng

et al.

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 69, P. 108266 - 108266

Published: Sept. 30, 2023

Language: Английский

Citations

24
CRISPR-Driven Biosensors: A New Frontier in Rapid and Accurate Disease Detection DOI

ZahraSadat Razavi,

M. Soltani, Mohammad Souri

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Sept. 17, 2024

This comprehensive review delves into the advancements and challenges in biosensing, with a strong emphasis on transformative potential of CRISPR technology for early rapid detection infectious diseases. It underscores versatility CRISPR/Cas systems, highlighting their ability to detect both nucleic acids non-nucleic acid targets, seamless integration isothermal amplification techniques. The provides thorough examination latest developments CRISPR-based biosensors, detailing unique properties such as high specificity programmability, which make them particularly effective detecting disease-associated acids. While focuses due its critical role diagnosing diseases, it also explores broader applications thereby acknowledging technology's potential. Additionally, identifies existing challenges, need improved signal real-world applicability, offers future perspectives aimed at overcoming these hurdles. ultimate goal is advance development highly sensitive specific biosensors that can be used widely improving human health, point-of-care settings resource-limited environments.

Language: Английский

Citations

15
Recent advances and challenges in plant viral diagnostics DOI Creative Commons

Aizada Kanapiya,

U.I. Amanbayeva,

Zhanar Tulegenova

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 13, 2024

Accurate and timely diagnosis of plant viral infections plays a key role in effective disease control maintaining agricultural productivity. Recent advances the viruses have significantly expanded our ability to detect monitor pathogens crops. This review discusses latest diagnostic technologies, including both traditional methods innovations. Conventional such as enzyme-linked immunosorbent assay DNA amplification-based assays remain widely used due their reliability accuracy. However, diagnostics next-generation sequencing CRISPR-based detection offer faster, more sensitive specific virus detection. The highlights main advantages limitations systems conventional methods, biosensor technologies advanced sequence-based techniques. In addition, it also effectiveness commercially available tools challenges facing modern techniques well future directions for improving informed management strategies. Understanding features methodologies would enable stakeholders choose optimal strategies against threats ensure global food security.

Language: Английский

Citations

7
CRISPR-assisted Diagnostics of Plant Viruses and Challenges DOI

Siddhant G. Jaybhaye,

R. L. Chavhan, V. R. Hinge

et al.

Virology, Journal Year: 2024, Volume and Issue: 597, P. 110160 - 110160

Published: June 26, 2024

Language: Английский

Citations

5
Multiplexed Detection Strategies for Biosensors Based on the CRISPR-Cas System DOI
Cong Wei,

Xueying Lei,

Songcheng Yu

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(6), P. 1633 - 1646

Published: June 11, 2024

A growing number of applications require simultaneous detection multiplexed nucleic acid targets in a single reaction, which enables higher information density combination with reduced assay time and cost. Clustered regularly interspaced short palindromic repeats (CRISPR) the CRISPR-Cas system have broad for acids due to their strong specificity, high sensitivity, excellent programmability. However, realizing is still challenging nonspecific collateral cleavage activity, limited signal reporting strategies, possible cross-reactions. In this review, we summarize principles, features based on further discuss challenges perspective.

Language: Английский

Citations

5
Multi-Level Biomarkers for Early Diagnosis of Ischaemic Stroke: A Systematic Review and Meta-Analysis DOI Open Access
Qianyun Li, Lingyun Zhao,

Ching Long Chan

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 13821 - 13821

Published: Sept. 7, 2023

Blood biomarkers hold potential for the early diagnosis of ischaemic stroke (IS). We aimed to evaluate current weight evidence and identify biological pathways further investigation. searched PubMed, EMBASE, Cochrane Library Web Science, used R package meta4diag diagnostic meta-analysis applied Gene Ontology (GO) analysis vital processes (BPs). Among 8544 studies, we included 182 articles with a total 30,446 participants: 15675 IS, 2317 haemorrhagic (HS), 1798 mimics, 846 transient attack 9810 control subjects. There were 518 pooled including 203 proteins, 114 genes, 108 metabolites 88 transcripts. Our study generated two shortlists future research: one optimal performance another low selection bias. Glial fibrillary acidic protein was eligible meta-analysis, summary sensitivities specificities differentiating HS from IS between 3 h 24 after onset ranging 73% 80% 77% 97%, respectively. GO revealed top five BPs associated IS. This provides holistic view in Two warrant

Language: Английский

Citations

10
Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies DOI Creative Commons
Chang He, Yongqi Li,

Jinkuan Liu

et al.

BME Frontiers, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Language: Английский

Citations

0