NLRP3 inflammasome activity and periodontal disease pathogenesis–A bidirectional relationship

Oral Diseases, Journal Year: 2024, Volume and Issue: 30(7), P. 4069 - 4077

Published: May 30, 2024

Periodontitis is an inflammatory oral disease that occurs as a result of the damaging effects immune response against subgingival microflora. Among mechanisms involved, nucleotide-binding oligomerization domain, leucine-rich repeat-containing proteins family member NLRP3 (NLR pyrin domain-containing 3), proposed key regulator macrophage-induced inflammation, strongly associated with periodontal due to bacterial activators. This paper aimed present general concepts inflammasome activation and regulation in disease.

Language: Английский

---

One‐stage versus two‐stage technique using two splinted extra‐short implants: A multicentric split‐mouth study with a one‐year follow‐up

Clinical Implant Dentistry and Related Research, Journal Year: 2022, Volume and Issue: 24(5), P. 602 - 610

Published: June 14, 2022

Abstract Objective To compare the clinical outcomes of extra‐short implants (≤6.5 mm) inserted with one‐stage versus two‐stage technique in adjacent sites upper or lower jaw. Materials and Methods In this split‐mouth multicenter study, were randomly divided into two groups according to healing phase: technique. Primary outcome measures implant survival, success, prosthodontic complications. Secondary measurements were: stability quotient (ISQ) collected at surgery time (T0), after 3 (T3) 12 (T12) months, marginal bone level (MBL) evaluated T0, T3, T6, T12, loss T6 plaque index (PI), probing depth (PD), bleeding on (BoP) T12. Significances differences between tested by linear mixed model random intercept. Results Nineteen patients ( 8 males 11 females) included. A total 38 inserted. At T12 cumulative survival success rate 100% both groups. No statistically significant recorded for any analyzed parameters point. ISQ values similar T0 (two‐stage: mean 67.53 ± SD 19.47; one‐stage: 66.53 19.07 p = 0.8738) increased 12‐month follow‐up appointment 81.1 7.04; 81.39 0.9266). MBL was 0.46 0.41 (two‐stage) 0.45 0.38 (one‐stage) mm 0.9417), while PD 2.7 0.85 2.69 0.89 mm. Conclusions Within limits present short‐term report, demonstrated optimal using technique, without compared traditional approach.

Language: Английский

---

Periodontitis and COVID-19: Immunological Characteristics, Related Pathways, and Association

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 3012 - 3012

Published: Feb. 3, 2023

Both periodontitis and Coronavirus disease 2019 (COVID-19) pose grave threats to public health social order, endanger human life, place a significant financial strain on the global healthcare system. Since COVID-19 pandemic, mounting research has revealed link between periodontitis. It is critical comprehend immunological mechanisms of two illnesses as well their interaction. Much evidence showed that there are many similar inflammatory pathways COVID-19, such NF-κB pathway, NLRP3/IL-1β IL-6 signaling pathway. Common risk factors gender, lifestyle, comorbidities contribute severity both diseases. Revealing internal relationship diseases conducive treatment in an emergency period. also maintain good oral hygiene positive attitude during treatment. This review covers four main areas: mechanisms, common factors, association diseases, possible interventions potential targets. These will provide ideas for drug development clinical

Language: Английский

---

Impact of local drug delivery and natural agents as new target strategies against periodontitis: new challenges for personalized therapeutic approach

Therapeutic Advances in Chronic Disease, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 1, 2023

Periodontitis is a persistent inflammation of the soft tissue around teeth that affects 60% population in globe. The self-maintenance inflammatory process can cause periodontal damage from alveolar bone resorption to tooth loss order contrast effects periodontitis, main therapy used scaling and root planing (SRP). At same time, studying physiopathology periodontitis has shown possibility using local drug delivery system as an adjunctive therapy. Using devices conjunction with SRP for potential tool since it increases efficacy minimizes negative by managing release. This review emphasized how use agents natural could be promising adjuvants treatment patients affected or not cardiovascular disease, diabetes, other problems. Moreover, evidences current issues new ideas inspire later study both basic research clinical practice tailored approach.

Language: Английский

---

Shared genetic architecture between periodontal disease and type 2 diabetes: a large scale genome-wide cross-trait analysis

Endocrine, Journal Year: 2024, Volume and Issue: 85(2), P. 685 - 694

Published: March 9, 2024

Abstract Purpose To investigate the relationship between abnormal glucose metabolism, type 2 diabetes (T2D), and periodontal disease (PER) independent of Body Mass Index (BMI), we employed a genome-wide cross-trait approach to clarify association. Methods Our study utilized most extensive association studies conducted for populations European ancestry, including PER, T2D, fasting glucose, insulin, 2-hour after an oral challenge, HOMA-β, HOMA-IR (unadjusted or adjusted BMI) HbA1c. Results With this approach, were able identify pleiotropic loci, establish expression-trait associations, quantify global local genetic correlations. There was significant positive correlation T2D (r g = 0.261, p 2.65 × 10 −13 ), HbA1c 0.182, 4.14 −6 ) as well BMI 0.158, 2.34 ). A also observed PER glycemic traits T2D. We identified 62 loci that impact both traits, Nine pathways shared genes PER. Genetically liability HOMA-βadjBMI causally associated with risk Conclusion research has revealed link is influenced by biological pleiotropy. Notably, some these links are not related BMI. highlights underlying patients regardless their

Language: Английский

---

Short term effect of antimicrobial photodynamic therapy and probiotic L. salivarius WB21 on halitosis: A controlled and randomized clinical trial

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0297351 - e0297351

Published: July 2, 2024

Objective This study aimed to evaluate the effect of antimicrobial photodynamic therapy (aPDT) and use probiotics on treatment halitosis. Methods Fifty-two participants, aged from 18 25 years, exhaling sulfhydride (H 2 S) ≥ 112 ppb were selected. They allocated into 4 groups (n = 13): Group 1: tongue scraper; 2: treated once with aPDT; 3: probiotic capsule containing Lactobacillus salivarius WB21 (6.7 x 10 8 CFU) xylitol (280mg), 3 times a day after meals, for 14 days; 4: aPDT days. Halimetry gas chromatography (clinical evaluation) microbiological samples collected dorsum before aPDT, as well 7, 14, 30 The clinical data failed follow normal distribution; therefore, comparisons made using Kruskal-Wallis test (independent measures) Friedman ANOVA (dependent followed by appropriate posthoc tests, when necessary. For data, seeing distribution, rank sum was performed Dunn’s post-test. significance level α 0.05. Results Clinical results (halimetry) showed an immediate significant reduction in halitosis (p 0.0008) and/or scraper 0.0006). Probiotics no difference relation initial levels 0.7530). No differences found control appointments. amount Porphyromonas gingivalis , Tannerella forsythia Treponema denticola not altered throughout analysis 0.1616, p 0.2829 0.2882, respectively). Conclusion There scraping, but there number bacteria study, or times, both results. New trials are necessary better assess tested therapies. Trial registration Trials NCT03996044 .

Language: Английский

---

Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering

Polymers, Journal Year: 2021, Volume and Issue: 13(13), P. 2114 - 2114

Published: June 28, 2021

Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been extensively studied for skin engineering. However, they showed rapid degradation and contraction over time low mechanical properties which limit their reproducibility lifespan. In order to achieve better properties, elasticity biological we incorporated a elastin-like recombinamer (ELR) network, two types of ELR, one modified with azide (SKS-N3) other cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 three different SKS (serine-lysine-serine sequence) concentrations (1, 3, 5 wt.%), into hydrogels. Our results decrease in gelation contraction, both the absence presence encapsulated human primary fibroblasts (hFBs), higher increase when SKSs content is equal or than 3%. hFBs proliferation an improvement lowest (1 wt.%) was used but started decreasing increasing concentration day 14 respect plasma control. Proliferation keratinocytes (hKCs) seeded top hybrid-plasma containing 1 3% no differences control hKCs observed wt.% SKS. These promising need balance between reduced indicate potential using this type hydrogel as testing platform pharmaceutical products cosmetics, future work will elucidate potential.

Language: Английский

---

Inflammasomes and the IL-1 Family in Bone Homeostasis and Disease

Current Osteoporosis Reports, Journal Year: 2022, Volume and Issue: 20(3), P. 170 - 185

Published: May 14, 2022

Abstract Purpose of Review Inflammasomes are multimeric protein structures with crucial roles in host responses against infections and injuries. The importance inflammasome activation goes beyond defense as a dysregulated subsequent secretion IL-1 family members is believed to be involved the pathogenesis various diseases, some which also produce skeletal manifestations. purpose this review summarize recent developments understanding regulation bone physiology pathology current therapeutics will discussed. Recent Findings Small animal models have been vital help understand how regulates dynamics. Animal gain or loss function components signaling illustrated these systems can impact numerous pathologies utilized test new therapeutics. Summary It increasingly clear that tightly regulated required not only for but homeostasis, linked diseases pathological accrual loss. Given complexities redundancies secretion, targeting pathways at times challenging. Ongoing research into inflammasome-mediated mechanisms allow development inflammasome/IL-1 diseases.

Language: Английский

---

Identification of hub cuproptosis related genes and immune cell infiltration characteristics in periodontitis

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 5, 2023

Periodontitis is an inflammatory disease and its molecular mechanisms not clear. A recently discovered cell death pathway called cuproptosis, may related to the disease.The datasets GSE10334 of human periodontitis control were retrieved from Gene Expression Omnibus database (GEO) for analysis.Following use two machine learning algorithms, least absolute shrinkage selection operator (LASSO) support vector machine-recursive feature removal (SVM-RFE) used find CRG-based signature. Then Receiver operating characteristic (ROC) curves was evaluate gene signature's discriminatory ability. The CIBERSORT deconvolution algorithm study link between hub genes distinct types immune cells. Next, association CRGs with cells in relevant clusters cuproptosis found. various ascertained by GSVA algorithm. Finally, An external dataset (GSE16134) confirm diagnosis capacity identified biomarkers. In addition, clinical samples examined using qRT-PCR immunohistochemistry verifiy expression cuprotosis signature better predict periodontitis.15 periodontitis-related DE-CRGs found,then 11-CRG-based found LASSO SVM-RFE. ROC also supported value results showed that a crucial component response.The NFE2L2, SLC31A1, FDX1,LIAS, DLD, DLAT, DBT highest levels Cluster2 ,while NLRP3, MTF1, DLST displayed lowest level Cluster 2 but Cluster1. 11 diagnostic regulate affecting biomarkers, verified these signiture periodontitis.These findings have important implications periodontitis, highlight further research needed understand underlying this relationship periodontitis.

Language: Английский

---

Growth differentiation factor-15 and circulating biomarkers as predictors of periodontal treatment effects in patients with periodontitis: a randomized-controlled clinical trial

BMC Oral Health, Journal Year: 2023, Volume and Issue: 23(1)

Published: Aug. 21, 2023

Abstract Background During the last decades, in patients with periodontitis, periodontal treatment has been shown to reduce potential release of local and systemic biomarkers linked an early risk inflammatory disorders. This study evaluated efficacy non-surgical-periodontal (NSPT) on growth differentiation factor 15 (GDF-15) related circulating such as glutathione peroxidase 1 (GPx-1), c-reactive protein (hs-CRP), surfactant D (SP-D) explored whether subjects who had high GDF-15 levels at baseline showed increased clinical benefits following NSPT 6-months follow-up. Methods For this two-arm, parallel randomized trial, periodontitis were randomly allocated receive quadrant scaling root-planing (Q-SRP, n = 23, median age 51 years old) or full-mouth disinfection (FMD, 50 treatment. Clinical parameters recorded all enrolled patients. The primary outcome was analyse serum concentrations changes GPx-1, hs-CRP, SP-D 30, 90, 180-days follow-up after through enzyme-linked immunosorbent assay (ELISA) nephelometric techniques. Results In comparison FMD, Q-SRP group a significant improvement (p < 0.05) reduction mean 0.005), hs-CRP 0.001), 0.042) increase GPx-1 0.025) 6 months At treatment, there association between several GDF-15, 0.05 for parameters). Finally, ANOVA analysis revealed that, significantly impacted 0.015), 0.026) upregulation 0.045). Conclusion results evidenced both protocols improved analyzed biomarkers, but more efficacious than FMD approach. Moreover, presented benefited from 6-month Trial registration NCT05720481.

Language: Английский

---