Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(15)

Published: May 26, 2023

Capillary electrophoresis is recognized as a valued separation technique for its high efficiency, low sample consumption, good economic and ecological aspects, reproducibility, complementarity to traditional liquid chromatography techniques. experiments are generally performed utilizing optical detection, such ultraviolet or fluorescence detectors. However, in order provide structural information, capillary hyphenated highly sensitive selective mass spectrometry has been developed overcome the limitations of detections. electrophoresis‐mass increasingly popular protein analysis, including biopharmaceutical biomedical research. It frequently applied determination physicochemical biochemical parameters proteins, offers excellent performance in‐depth characterizations biopharmaceuticals at various levels also already proven promising tool biomarker discovery. In this review, we focus on possibilities analysis their intact level. Various modes interfaces, well approaches prevent adsorption enhance loading capacity, discussed recent (2018–March 2023) developments applications field summarized.

Language: Английский

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Current green capillary electrophoresis and liquid chromatography methods for analysis of pharmaceutical and biomedical samples (2019–2023) – A review

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1323, P. 342889 - 342889

Published: June 20, 2024

Separation analytical methods, including liquid chromatography (LC) and capillary electrophoresis (CE), in combination with an appropriate detection technique, are dominant powerful approaches preferred the analysis of pharmaceutical biomedical samples. Recent trends methods focused on activities that push them to field greenness sustainability. New based implementation greener solvents, non-hazardous chemicals, reagents have grown exponentially. Similarly, recent pushed strategies miniaturization, reduction wastes, avoiding derivatization procedures, or energy consumption. However, real method can be evaluated only according objective sufficient metric offering complex results taking into account all twelve rules green chemistry (SIGNIFICANCE mnemonic system). This review provides extensive overview papers published area development LC CE over last 5 years (2019-2023). The main focus is situated metrics used for evaluation applied determination bioactive agents. It critically evaluates compares demands applicability quality control clinical environment requirements (GAC). Greenness practicality summarized re-evaluated newly use tools, i.e., Analytical GREEnness (AGREE), Green Procedure Index (GAPI), Blue Applicability Grade (BAGI), Sample Preparation Metric Sustainability (SPMS). Moreover, general conclusions future perspectives greening procedures systems presented. paper should provide comprehensive information chemists, biochemists, it also represent a valuable source clinicians, laboratories interested greenness, practicality,

Language: Английский

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A Novel Peptide Mapping Method Utilizing Cysteine as a Reducing Agent

Pharmaceutical Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Language: Английский

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Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(12)

Published: Feb. 26, 2023

This review gives a wide overview of recent advances and applications capillary electrophoresis microchip methods in the fields proteomics peptidomics period from mid‐2018 up to end 2022. The methodological topics covering sample preparation concentration techniques, hyphenation with mass spectrometry, multidimensional separations by on‐line or off‐line coupled different liquid chromatography techniques are described new developments both bottom‐up top‐down approaches presented. In addition, various proteomic peptidomic studies demonstrated. They include monitoring protein posttranslational modifications biological biochemical research, clinical proteomics, food analysis.

Language: Английский

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 165 - 198

Published: Sept. 5, 2023

Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.

Language: Английский

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Recent developments of imaged capillary isoelectric focusing technology for in-depth biopharmaceutical characterization

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118212 - 118212

Published: Feb. 1, 2025

Language: Английский

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Saccharide mapping as an extraordinary method on characterization and identification of plant and fungi polysaccharides: A review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133350 - 133350

Published: Aug. 1, 2024

Language: Английский

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Buffering agents and additives for the background electrolyte solutions used for peptide and protein capillary zone electrophoresis

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 164, P. 117080 - 117080

Published: May 6, 2023

The background electrolyte solution (BGE) has a strong impact on the separation performance of peptides and proteins in all techniques governed by electrophoretic principles, due to its considerable influence mechanism physicochemical properties both sample inner surface device, which mostly consists fused-silica capillary tube. This review article examines discusses effects composition pH BGE electrokinetic phenomena taking place at solid liquid interface within capillaries, generating electroosmotic flow (EOF), zone electrophoresis (CZE), is most used mode for these biopolymers. paper reviews literature relevant explain how pH, ionic strength, conductivity employed peptide protein CZE efficiency, selectivity resolution other factors affecting separation, including flow. Also examined discussed compatibility with ionization sources on-line hyphenation mass spectrometry two popular preconcentration methods field-amplified stacking (FASS) dynamic junction.

Language: Английский

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Greening Separation and Purification of Proteins and Peptides

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(19)

Published: Oct. 1, 2024

The increasing awareness of environmental issues and the transition to green analytical chemistry (GAC) have gained popularity among academia industry in recent years. One principles GAC is reduction replacement toxic solvents with more sustainable environmentally friendly ones. This review gives an overview advances applying as alternative traditional organic for peptide protein purification analysis by liquid chromatography (LC) capillary electrophoresis (CE) methods. feasibility using greener LC CE methods demonstrated through several application examples; however, there still plenty room new developments fully realize their potential address existing challenges. Thanks tunable properties designer solvents, such ionic liquids deep eutectic almost infinite possible mixtures components production, it that some could potentially surpass harmful future. Therefore, future research should focus mainly on developing solvent combinations enhancing instruments be able effectively work solvents.

Language: Английский

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Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(16)

Published: Aug. 1, 2024

This review provides an overview of recent works focusing on the determination amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection ultraviolet (UV) detection, which is most widespread in electromigration techniques, without pre-capillary derivatization. Available options for UV these analytes, such as indirect complexation transition metal ions, in-capillary derivatization are described. Developments field direct UV-absorbing AAs well progress chiral separation A separate section dedicated to on-line sample preconcentration methods combined electrophoresis-UV.

Language: Английский

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