Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112583 - 112583
Published: Dec. 1, 2024
Language: Английский
Microchemical Journal, Journal Year: 2024, Volume and Issue: 204, P. 111124 - 111124
Published: Sept. 1, 2024
Language: Английский
Citations
5
Journal of Liquid Chromatography & Related Technologies, Journal Year: 2023, Volume and Issue: 46(6-10), P. 153 - 167
Published: June 21, 2023
Zibushengfa Tablet is a widely consumed traditional Chinese medicine (TCM) prescription. However, its main bioactives remain unclear and pharmacopeia quality-marker (Q-marker) poorly characterized nowadays. The present study analyzed lyophilized aqueous powder using ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry. Through comparison with authentic standards in the library, 46 were putatively identified, including quinic acid, D-gluconic gallic protocatechuic 5-caffeoylquinic L-tryptophan, salidroside, (+)-catechin hydrate, caffeic cryptochlorogenic mangiferin, ferulic myricetin-3-O-galactoside, 2,3,5,4'-tetrahydroxystilbene-2-O-glucoside, forsythoside B, myricitrin, rutin, hyperoside, acteoside, 3,4-dicaffeoylquinic myricetin, senkyunolide A, isochlorogenic acid C, quercitrin, (+)-epipinoresinol, quercetin, biochanin calycosin, luteolin, wedelolactone, calycosin-7-O-glucoside, chrysoeriol, trans-cinnamic formononetin, chrysin, 3,3',4',5,6,7,8-heptamethoxyflavone, Z-ligustilide, amentoflavone, tangeretin, hinokiflavone, emodin, physcion, linoleic stearic palmitic ethyl ester. Of these, four pairs of isomers successfully differentiated newly established library-filtering strategy. On this basis, wedelolactone are preliminarily suggested as additional quality-markers for consideration by Pharmacopeia Committee, to prevent possible adulterate.
Language: Английский
Citations
11
Pharmacognosy Magazine, Journal Year: 2025, Volume and Issue: unknown
Published: March 24, 2025
Background: There is an abnormal enhancement of androgen receptor activity in prostate cancer cells, leading to increased sensitivity androgens and the promotion cell proliferation. Purpose: This study aimed investigate effects Eucommia ulmoides leaf-derived kaempferol extract on biological cycle androgen-dependent cells (LNCaP) through vitro culture experiments. Methods: Kaempferol was isolated extracted from hydrolysate E. leaves, its clearance rates for superoxide anion (O 2 − ) hydroxyl radical (·OH) were analyzed. Subsequently, LNCaP treated with at concentrations 0.0, 0.5, 1.0, 2.0 µmol L⁻¹ grouped accordingly. Changes proliferation inhibition rate, apoptosis distribution, levels apoptosis-related proteins assessed. Results: The recovery rate leaves approximately 98.23%, contents 0.008% before hydrolysis 0.089% after hydrolysis. As concentration increased, clearing (CR) O ·OH free radicals also gradually ( p < .05). In contrast 0.0 group, groups have exhibited greatly enhanced LNCaP, a remarkable decrease S-phase proportion, substantial increase G2/M-phase significant reduction Bcl-2 protein. Conversely, expressions Bax, caspase-3, caspase-9 significantly upregulated Conclusion: demonstrated pronounced anti-oxidant effect. found inhibit promote by modulating proteins.
Language: Английский
Citations
0
Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(17)
Published: July 14, 2023
The chemical constituents from Phellodendron amurense Rupr. were characterized systematically by ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry method for collecting mass data, and the fingerprints was established, providing reference its quality control. chromatographic column ACQUITY UPLC BEH-C18 (100 mm×2.1 mm, 1.7 μm). mobile phase acetonitrile-0.1% formic acid aqueous solution compounds P. identified Qualitative Analysis 10.0 software, substance, retention time, fragmentation pattern database retrieval. Meanwhile, chromatography-mass fingerprint methods of chinense Schneid. established using similarity evaluation system traditional Chinese medicine (2012 edition), differences analyzed multivariate statistical analysis methods. A total 105 identified, including 102 alkaloids, two phenolic acids, one lactone compound. Liquid with ideal precision, stability repeatability, 12 differential markers recognized between above herbs. can be used qualitative Rupr., clarifying material basis promoting clinical precision medication
Language: Английский
Citations
7
Rapid Communications in Mass Spectrometry, Journal Year: 2024, Volume and Issue: 38(13)
Published: May 1, 2024
Perillae Fructus (PF) is a common traditional Chinese medicine (TCM) for the treatment of asthma. It has not been effectively characterized by rosmarinic acid (RosA), which currently designed as sole quality indicator in Pharmacopoeia.
Language: Английский
Citations
2
Chromatographia, Journal Year: 2024, Volume and Issue: 87(9), P. 597 - 608
Published: July 4, 2024
Language: Английский
Citations
2
Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 27
Published: July 3, 2023
Myricitrin is a member of flavonols, natural phenolic compounds extracted from plant resources. It has gained great attention for various biological activities, such as anti-inflammatory, anti-cancer, anti-diabetic, well cardio-/neuro-/hepatoprotective activities. These effects have been demonstrated in both vitro and vivo models, making myricitrin favorable candidate the exploitation novel functional foods with potential protective or preventive against diseases. This review summarized health benefits attempted to uncover its action mechanism, expecting provide theoretical basis their application. Despite enormous bioactive myricitrin, low production, high cost, environmental damage caused by extracting it resources greatly constrain practical Fortunately, innovative, green, sustainable extraction techniques are emerging extract which function alternatives conventional techniques. Additionally, biosynthesis based on synthetic biology plays an essential role industrial-scale manufacturing, not reported exclusively. The construction microbial cell factories absolutely appealing competitive option produce large-scale manufacturing. Consequently, state-of-the-art green trends were reviewed discussed endow innovative perspective production myricitrin.
Language: Английский
Citations
6
Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(19)
Published: July 31, 2023
Chemome characterization is the prerequisite for either therapeutic mechanism clarification or quality control of traditional Chinese medicine prescriptions (TCMPs). Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) currently serves as most popular analytical tool; however, chemome still challenged by MS/MS spectral acquisition and post-acquisition data processing. Here, an integrated strategy was proposed in-depth Shengbai oral liquid (SBOL). Gas phase ion fractionation with staggered mass ranges demonstrated to be superior method regarding MS2 spectrum coverage in this study, narrower range further advanced coverage. To facilitate information extraction, all ingredient materials were measured parallel form in-house library, where each MS1 -MS2 item generated a square mass-to-charge ratio (m/z) frame capture tagged identity chemical family produced pentagon defect features accomplish analogs-targeted quasi-molecular extraction. Square m/z imprinting captured 355 identities, while frames extracted 275 compounds. Attributing comprehensive efficient processing, components tentatively identified, resulting clarified composition SBOL. Therefore, should meaningful TCMPs.
Language: Английский
Citations
1
Molecules, Journal Year: 2024, Volume and Issue: 29(20), P. 4886 - 4886
Published: Oct. 15, 2024
To date, no study has focused on
Language: Английский
Citations
0
Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112583 - 112583
Published: Dec. 1, 2024
Language: Английский
Citations
0