Enzyme-Based Anti-Inflammatory Therapeutics for Inflammatory Diseases DOI Creative Commons
Kannan Badri Narayanan

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(5), P. 606 - 606

Published: May 2, 2025

Inflammation is a multifaceted biological response of the immune system against various harmful stimuli, including pathogens (such as bacteria and viruses), cellular damage, toxins, natural/synthetic irritants. This protective mechanism essential for eliminating cause injury, removing damaged cells, initiating repair process. While inflammation fundamental component body’s defense healing process, its dysregulation can lead to pathological consequences, contributing acute chronic diseases, such autoimmune disorders, cancer, metabolic syndromes, cardiovascular neurodegenerative conditions, other systemic complications. Generally, non-steroidal anti-inflammatory drugs (NSAIDs), corticosteroids, disease-modifying anti-rheumatic (DMARDs), antihistamines, biologics, colchicine are used pharmacological agents in management inflammatory diseases. However, these conventional treatments often have limitations, adverse side effects, long-term toxicity, drug resistance. In contrast, enzyme-based therapeutics emerged promising alternative due their high specificity, catalytic efficiency, ability modulate pathways with reduced effects. These enzymes function by scavenging reactive oxygen species (ROS), inhibiting cytokine transcription, degrading circulating cytokines, blocking release targeting exocytosis-related receptors. Additionally, role tissue regeneration further enhances therapeutic potential. Most natural belong oxidoreductase class, catalase superoxide dismutase, well hydrolases trypsin, chymotrypsin, nattokinase, bromelain, papain, serratiopeptidase, collagenase, hyaluronidase, lysozyme. Engineered enzymes, Tobacco Etch Virus (TEV) protease botulinum neurotoxin type A (BoNT/A), also demonstrated significant potential targeted therapies. Recent advancements enzyme engineering, nanotechnology-based delivery, biopharmaceutical formulations expanded applicability treating review provides comprehensive overview both engineered along formulations, therapeutics. It highlights improvements stability, efficacy, minimized immunogenicity, while discussing mechanisms action clinical applications future developments biomedical

Language: Английский

Nanoparticles in tumor microenvironment remodeling and cancer immunotherapy DOI Creative Commons
Qiang Lü,

Dongquan Kou,

Shenghan Lou

et al.

Journal of Hematology & Oncology, Journal Year: 2024, Volume and Issue: 17(1)

Published: April 2, 2024

Abstract Cancer immunotherapy and vaccine development have significantly improved the fight against cancers. Despite these advancements, challenges remain, particularly in clinical delivery of immunomodulatory compounds. The tumor microenvironment (TME), comprising macrophages, fibroblasts, immune cells, plays a crucial role response modulation. Nanoparticles, engineered to reshape TME, shown promising results enhancing by facilitating targeted These nanoparticles can suppress fibroblast activation, promote M1 macrophage polarization, aid dendritic cell maturation, encourage T infiltration. Biomimetic further enhance increasing internalization agents cells such as cells. Moreover, exosomes, whether naturally secreted body or bioengineered, been explored regulate TME immune-related affect cancer immunotherapy. Stimuli-responsive nanocarriers, activated pH, redox, light conditions, exhibit potential accelerate co-application with checkpoint inhibitors is an emerging strategy boost anti-tumor immunity. With their ability induce long-term immunity, nanoarchitectures are structures development. This review underscores critical overcoming current driving advancement modification.

Language: Английский

Citations

135
Signaling pathways in macrophages: molecular mechanisms and therapeutic targets DOI Creative Commons
Ming Li, Mengjie Wang, Yuanjia Wen

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(5)

Published: Sept. 11, 2023

Abstract Macrophages play diverse roles in development, homeostasis, and immunity. Accordingly, the dysfunction of macrophages is involved occurrence progression various diseases, such as coronavirus disease 2019 atherosclerosis. The protective or pathogenic effect that exert different conditions largely depends on their functional plasticity, which regulated via signal transduction Janus kinase–signal transducer activator transcription, Wnt Notch pathways, stimulated by environmental cues. Over past few decades, molecular mechanisms signaling pathways have been gradually elucidated, providing more alternative therapeutic targets for diseases treatment. Here, we provide an overview basic physiology expound regulatory within them. We also address crucial role pathogenesis including autoimmune, neurodegenerative, metabolic, infectious cancer, with a focus advances macrophage‐targeted strategies exploring modulation components regulators pathways. Last, discuss challenges possible solutions therapy clinical applications. hope this comprehensive review will directions further research targeting macrophage are promising to improve efficacy

Language: Английский

Citations

46
Probiotics in Wound Healing DOI Open Access

Valentina Alexandra Bădăluță,

Carmen Curuțiu, Lia-Mara Dițu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5723 - 5723

Published: May 24, 2024

Wound infections caused by opportunistic bacteria promote persistent infection and represent the main cause of delayed healing. Probiotics are acknowledged for their beneficial effects on human body could be utilized in management various diseases. They also possess capacity to accelerate wound healing, due remarkable anti-pathogenic, antibiofilm, immunomodulatory effects. Oral topical probiotic formulations have shown promising openings field dermatology, there vitro vivo models focusing healing mechanisms. dressings embedded with prebiotics probiotics now prime candidates designing therapeutic approaches combat process. The aim this review is conduct an extensive scientific literature regarding efficacy oral management, as well potential dressing embedding pre- stimulating

Language: Английский

Citations

16
Role of macrophage in intervertebral disc degeneration DOI Creative Commons

Yiming Dou,

Yiming Zhang, Yang Liu

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Language: Английский

Citations

2
Structure-activity relationships of bioactive polysaccharides extracted from macroalgae towards biomedical application: A review DOI

Yinyi Fu,

Haixin Jiao, Jianzhong Sun

et al.

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 324, P. 121533 - 121533

Published: Oct. 26, 2023

Language: Английский

Citations

33
Single-cell histone chaperones patterns guide intercellular communication of tumor microenvironment that contribute to breast cancer metastases DOI Creative Commons
Jindong Xie, Wei Deng, Xinpei Deng

et al.

Cancer Cell International, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 6, 2023

Histone chaperones (HCs) are crucial for governing genome stability and gene expression in multiple cancers. However, the functioning of HCs tumor microenvironment (TME) is still not clearly understood.Self-tested single-cell RNA-seq data derived from 6 breast cancer (BC) patients with brain liver metastases were reanalyzed by nonnegative matrix factorization (NMF) algorithm 36 HCs. TME subclusters observed BC immunotherapy public cohorts to assess their prognosis immune response. The biological effect HSPA8, one HCs, was verified transwell assay wound-healing assays.Cells including fibroblasts, macrophages, B cells, T classified into various based on marker genes. Additionally, it showed that might be strongly associated clinical features metastases, along pseudotime trajectory each cell type. Besides, results bulk-seq analysis revealed mediated distinguished significant prognostic value relevant patients' responses, especially cells macrophages. In particular, CellChat exhibited HCs-related extensive diverse interactions malignant cells. Finally, assays HSPA8 deficiency inhibited migration invasion.Collectively, our study first dissected HCs-guided intercellular communication contribute metastases.

Language: Английский

Citations

17
Macrophages and Gut Barrier Function: Guardians of Gastrointestinal Health in Post-Inflammatory and Post-Infection Responses DOI Open Access

Edward Xiangtai Meng,

G. Nicholas Verne, QiQi Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9422 - 9422

Published: Aug. 30, 2024

The gut barrier is essential for protection against pathogens and maintaining homeostasis. Macrophages are key players in the immune system, indispensable intestinal health, contribute to defense repair mechanisms. Understanding multifaceted roles of macrophages can provide critical insights into restoring gastrointestinal (GI) health. This review explores role function their contribution post-inflammatory post-infectious responses gut. significantly integrity through epithelial repair, modulation, interactions with microbiota. They demonstrate active plasticity by switching phenotypes resolve inflammation, facilitate tissue regulate microbial populations following an infection or inflammation. In addition, tissue-resident (M2) infiltration (M1) convert each other problems such as IBS IBD via major signaling pathways mediated NF-κB, JAK/STAT, PI3K/AKT, MAPK, Toll-like receptors, specific microRNAs miR-155, miR-29, miR-146a, miR-199, which may be good targets new therapeutic approaches. Future research should focus on elucidating detailed molecular mechanisms developing personalized approaches fully harness potential maintain restore permeability

Language: Английский

Citations

6
CAR-macrophage: Breaking new ground in cellular immunotherapy DOI Creative Commons

Ting Huang,

Chenqi Bei,

Zhenhua Hu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 3, 2024

Chimeric Antigen Receptor (CAR) technology has revolutionized cellular immunotherapy, particularly with the success of CAR-T cells in treating hematologic malignancies. However, have limited efficacy against solid tumors. To address these limitations, CAR-macrophages (CAR-Ms) leverage innate properties macrophages specificity and potency CAR technology, offering a novel promising approach to cancer immunotherapy. Preclinical studies shown that CAR-Ms can effectively target destroy tumor cells, even within challenging microenvironments, by exhibiting direct cytotoxicity enhancing recruitment activation other immune cells. Additionally, favorable safety profile their persistence tumors position as potentially safer more durable therapeutic options compared This review explores recent advancements including engineering strategies optimize anti-tumor preclinical evidence supporting use. We also discuss challenges future directions developing therapies, emphasizing potential revolutionize By harnessing unique macrophages, offer groundbreaking overcoming current limitations cell paving way for effective sustainable treatments.

Language: Английский

Citations

6
Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights DOI Creative Commons
Cheima Djehiche,

Nadia Benzidane,

Hanène Djeghim

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(3), P. 385 - 385

Published: March 18, 2024

Ammodaucus leucotrichus exhibits promising pharmacological activity, hinting at anti-inflammatory and anti-arthritic effects. This study investigated seed extracts from using methanol n-hexane, focusing on properties. The extract outperformed the n-hexane diclofenac, a reference drug, in trypsin inhibition (85% vs. 30% 64.67% 125 μg/mL). For inhibition, IC50 values were 82.97 μg/mL (methanol), 202.70 (n-hexane), 97.04 (diclofenac). Additionally, surpassed diclofenac BSA (bovine serum albumin) denaturation (90.4% 22.0% 51.4% 62.5 14.30 5408 42.30 Gas chromatography-mass spectrometry (GC-MS) revealed 59 58 secondary metabolites extracts, respectively. higher therapeutic activity of was attributed to hydroxyacetic acid hydrazide, absent extract. In silico docking studies identified 28 compounds with negative binding energies, indicating potential inhibition. 2-hydroxyacetohydrazide displayed superior inhibitory effects compared diclofenac. Further mechanistic are crucial validate as drug candidate for rheumatoid arthritis treatment.

Language: Английский

Citations

5
Macrophage membrane-coated nanoparticles for the treatment of infectious diseases DOI
Chenguang Wang,

Chuyu Li,

Ruoyu Zhang

et al.

Biomedical Materials, Journal Year: 2024, Volume and Issue: 19(4), P. 042003 - 042003

Published: May 13, 2024

Abstract Infectious diseases severely threaten human health, and traditional treatment techniques face multiple limitations. As an important component of immune cells, macrophages display unique biological properties, such as biocompatibility, immunocompatibility, targeting specificity, immunoregulatory activity, play a critical role in protecting the body against infections. The macrophage membrane-coated nanoparticles not only maintain functions inner but also inherit characteristics macrophages, making them excellent tools for improving drug delivery therapeutic implications infectious (IDs). In this review, we describe their advantages challenges ID therapy. We first summarize pathological features IDs, providing insight into how to fight them. Next, focus on classification, characteristics, preparation nanoparticles. Finally, comprehensively progress combating including delivery, inhibition killing pathogens, modulation. At end look forward aspect is presented.

Language: Английский

Citations

5