Strategies for improving antimicrobial peptide production

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 59, P. 107968 - 107968

Published: April 28, 2022

Language: Английский

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Antimicrobial Peptides: From Design to Clinical Application

Antibiotics, Journal Year: 2022, Volume and Issue: 11(3), P. 349 - 349

Published: March 6, 2022

Infection of multidrug-resistant (MDR) bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Enterobacteriaceae (CRE), and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, brings public health issues causes economic burden. Pathogenic bacteria develop several methods to resist antibiotic killing or inhibition, mutation function sites, activation drug efflux pumps, enzyme-mediated degradation. Antibiotic resistance components can be transferred between by mobile genetic elements including plasmids, transposons, integrons, well bacteriophages. The development limits the treatment options for bacterial infection, especially MDR bacteria. Therefore, novel alternative antibacterial agents are urgently needed. Antimicrobial peptides (AMPs) display multiple mechanisms against infections, directly bactericidal activity immunomodulatory function, potential alternatives antibiotics. In this review, resistance, AMPs, especially, design, optimization, delivery AMPs reviewed. Strategies structural change, amino acid substitution, conjugation with cell-penetration peptide, terminal acetylation amidation, encapsulation nanoparticles will improve antimicrobial efficacy, reduce toxicity, accomplish local AMPs. addition, clinical trials in AMP studies applications within last five years were summarized. Overall, diverse action infection pathogenic future research investigations accelerate application.

Language: Английский

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Flourishing Antibacterial Strategies for Osteomyelitis Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 10(11)

Published: Jan. 30, 2023

Abstract Osteomyelitis is a destructive disease of bone tissue caused by infection with pathogenic microorganisms. Because the complex and long‐term abnormal conditions, osteomyelitis one refractory diseases in orthopedics. Currently, anti‐infective therapy primary modality for addition to thorough surgical debridement. However, bacterial resistance has gradually reduced benefits traditional antibiotics, development advanced antibacterial agents received growing attention. This review introduces main targets treating osteomyelitis, including cell wall, membrane, intracellular macromolecules, energy metabolism, focuses on their mechanisms, predicts prospects clinical applications.

Language: Английский

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Antimicrobial Natural Hydrogels in Biomedicine: Properties, Applications, and Challenges—A Concise Review

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2191 - 2191

Published: Jan. 22, 2023

Natural hydrogels are widely used as biomedical materials in many areas, including drug delivery, tissue scaffolds, and particularly wound dressings, where they can act an antimicrobial factor lowering the risk of microbial infections, which serious health problems, especially with respect to healing. In this review article, a number promising strategies development biocidal properties, those originating from natural polymers, briefly summarized concisely discussed. Common design fabricate intrinsic or stimuli-triggered antibacterial activity exemplified, mechanisms lying behind these properties also Finally, practical applications considered while discussing current challenges perspectives.

Language: Английский

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Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria

Journal of Biomedical Science, Journal Year: 2023, Volume and Issue: 30(1)

Published: April 26, 2023

Gram-positive (G+) bacterial infection is a great burden to both healthcare and community medical resources. As result of the increasing prevalence multidrug-resistant G+ bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), novel antimicrobial agents must urgently be developed for treatment infections caused by bacteria. Endolysins are bacteriophage (phage)-encoded enzymes that can specifically hydrolyze cell wall quickly kill Bacterial resistance endolysins low. Therefore, considered promising alternatives solving mounting problem. In this review, derived from phages targeting were classified based on their structural characteristics. The active mechanisms, efficacy, advantages antibacterial drug candidates summarized. Moreover, remarkable potential phage in was described. addition, safety endolysins, challenges, possible solutions addressed. Notwithstanding limitations trends development indicate endolysin-based drugs will approved near future. Overall, review presents crucial information current progress involving therapeutic agents, it provides guideline biomaterial researchers who devoting themselves fighting against infections.

Language: Английский

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Photothermal microneedle patch loaded with antimicrobial peptide/MnO2 hybrid nanoparticles for chronic wound healing

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 482, P. 148938 - 148938

Published: Jan. 21, 2024

Language: Английский

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Photochemical Strategies toward Precision Targeting against Multidrug-Resistant Bacterial Infections

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14085 - 14122

Published: May 22, 2024

Infectious diseases pose a serious threat and substantial economic burden on global human public health security, especially with the frequent emergence of multidrug-resistant (MDR) bacteria in clinical settings. In response to this urgent need, various photobased anti-infectious therapies have been reported lately. This Review explores discusses several photochemical targeted antibacterial therapeutic strategies for addressing bacterial infections regardless their antibiotic susceptibility. contrast conventional therapies, these approaches facilitate precise targeting pathogenic and/or infectious microenvironments, effectively minimizing toxicity mammalian cells surrounding healthy tissues. The highlighted include photodynamic therapy, photocatalytic photothermal endogenous pigments-based photobleaching polyphenols-based photo-oxidation therapy. comprehensive exploration aims offer updated information development effective, convenient, safe, alternative counter growing MDR future.

Language: Английский

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Synergistic action of antimicrobial peptides and antibiotics: current understanding and future directions

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 31, 2024

Antibiotic resistance is a growing global problem that requires innovative therapeutic approaches and strategies for administering antibiotics. One promising approach combination therapy, in which two or more drugs are combined to combat an infection. Along this line, the of antimicrobial peptides (AMPs) with conventional antibiotics has gained attention mainly due complementary mechanisms action AMPs In article, we review both

Language: Английский

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Enhancing Antimicrobial Peptide Activity through Modifications of Charge, Hydrophobicity, and Structure

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10821 - 10821

Published: Oct. 9, 2024

Antimicrobial peptides (AMPs) are emerging as a promising alternative to traditional antibiotics due their ability disturb bacterial membranes and/or intracellular processes, offering potential solution the growing problem of antimicrobial resistance. AMP effectiveness is governed by factors such net charge, hydrophobicity, and form amphipathic secondary structures. When properly balanced, these characteristics enable AMPs selectively target while sparing eukaryotic cells. This review focuses on roles positive structure in influencing activity toxicity, explores strategies optimize them for enhanced therapeutic potential. We highlight delicate balance between properties how various modifications, including amino acid substitutions, peptide tagging, or lipid conjugation, can either enhance impair performance. Notably, an increase parameters does not always yield best results; sometimes, slight reduction structural stability improves overall Understanding complex interactions key developing with greater reduced making viable candidates fight against antibiotic-resistant bacteria.

Language: Английский

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Bacteriophage therapy for drug-resistant Staphylococcus aureus infections

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 31, 2024

Drug-resistant Staphylococcus aureus stands as a prominent pathogen in nosocomial and community-acquired infections, capable of inciting various infections at different sites patients. This includes bacteremia (SaB), which exhibits severe infection frequently associated with significant mortality rate approximately 25%. In the absence better alternative therapies, antibiotics is still main approach for treating infections. However, excessive use has, turn, led to an increase antimicrobial resistance. Hence, it imperative that new strategies are developed control drug-resistant S. Bacteriophages viruses ability infect bacteria. Bacteriophages, were used treat bacterial before advent antibiotics, but subsequently replaced by due limited theoretical understanding inefficient preparation processes time. Recently, phages have attracted attention many researchers again because serious problem antibiotic article provides comprehensive overview phage biology, animal models, diverse clinical case treatments, trials context therapy. It also assesses strengths limitations therapy outlines future prospects research directions. review expected offer valuable insights engaged phage-based treatments

Language: Английский

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