Photoreactivation Activities of Rad5, Rad16A and Rad16B Help Beauveria bassiana to Recover from Solar Ultraviolet Damage DOI Creative Commons

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 420 - 420

Published: June 13, 2024

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair nucleotide excision of ultraviolet-induced DNA damage; however, their homologs have not yet been studied non-yeast fungi. fungus Beauveria bassiana, a deficiency Rad7 homolog, ortholog two Rad16 paralogs (Rad16A/B) instituted an ability to help insect-pathogenic recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were altered absence rad5, rad16A or rad16B, while severe defects growth conidiation caused by double deletion rad16B. Compared with wild-type complemented strains, mutants showed differentially reduced activities regarding resilience UVB-impaired conidia at 25 °C 12-h incubation regime visible light plus dark (L/D 3:9 h 5:7 for photoreactivation) full darkness (dark reactivation) mimicking natural nighttime. estimates median lethal dose LD50 L/D treatments revealed greater Rad16B than Rad16A additive either NER-dependent reactivation photorepair-dependent However, limited recovering low dose-impaired but unable impaired sublethal doses as did photoreactivation 5:7, unless night/dark time was doubled further prolonged. Therefore, anti-UV effects Rad5, B. bassiana depend primarily on are mechanistically distinct those yeast homologs.

Language: Английский

Evaluating Beauveria bassiana Strains for Insect Pest Control and Endophytic Colonization in Wheat DOI Creative Commons
Lu‐Lu Liu, Shiming Liu,

Qingfan Meng

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 287 - 287

Published: March 10, 2025

Certain entomopathogenic fungi, such as Beauveria bassiana, are highly pathogenic to arthropod pests and able colonize plant tissues, thereby enhancing both growth disease resistance. This study assessed three B. bassiana strains (CBM1, CBM2, CBM3) for their pathogenicity toward insect larvae colonization potential in wheat. The insecticidal activity of the fungi against major lepidopteran Helicoverpa armigera, Spodoptera frugiperda, Mythimna separata, Plutella xylostella was determined. were then applied wheat plants using seed immersion soil drench methods; rates compared, impacts fungal on survival evaluated. results demonstrated that all effective reducing damage, with CBM1 exhibiting highest followed by CBM3 CBM2. particularly effective, a significantly higher rate achieved through drenching compared immersion. inoculation resulted increased height at 30 days after sowing (DAS) root length 15 DAS control group. CBM1-colonized mortality fall armyworm. research has enriched biological microbial resource pool highlights integrated pest management strategies.

Language: Английский

Citations

0
The multifunctionality of the fungus Metarhizium spp. and its use in Brazilian agriculture DOI Open Access
H. A. Boaventura,

E. D. Quintela

Bragantia, Journal Year: 2025, Volume and Issue: 84

Published: Jan. 1, 2025

ABSTRACT The genus Metarhizium is extremely diverse, with 66 species described worldwide, among which 60 are entomopathogenic. Despite the diversity of 14 found in Brazil, anisopliae fungal highest number registered products. It an active ingredient 100 products, while rileyi only four. This was first on-farm-produced fungus Brazil by sugarcane mills 1960s to control leafhoppers. Currently, more than 50 on-farm biofactories produce this for application on 2 million hectares, making it largest microbial program world. Brazilian producers have often used fungi a mixture synthetic chemical insecticides, aiming at shock action and residual biological (55% use biocontrol agents chemicals pest control). impact M. as mycoinsecticide has recently been enhanced discovery that colonizes plant roots, can exploit its ability disease, promote growth, bioprotectant. systematic review demonstrates versatile adaptable diverse environments remarkable genetic, physiological, ecological flexibility, most widely control. In addition, prospects future studies increasing mitigate damage caused pests crops discussed.

Language: Английский

Citations

0
Timing of Fungal Insecticide Application to Avoid Solar Ultraviolet Irradiation Enhances Field Control of Rice Planthoppers DOI Creative Commons
Wanying Xu,

Zhen-Xin Wen,

Xinjie Li

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(4), P. 307 - 307

Published: March 23, 2023

Thechemical control of rice planthoppers (RPH)is prohibited in annual rice–shrimp rotation paddy fields. Here, the fungal insecticides Beauveria bassiana ZJU435 and Metarizhium anisoplae CQ421 were tested for RPH populations dominated by Nilaparvata lugens three field trials. During four-week trials initiated from harsh weather high temperatures strong sunlight, crop at stages tillering to flowering was effectively protected sprays applied 14-day intervals. The either insecticide after 5:00 p.m. (solar UV avoidance) suppressed population better than those before 10 a.m. avoidance versus exposure resulted mean efficacies 60% 56% 41% 45% on day 7, 77% 78% 63% 67% 14, 84% 82% 80% 79% 21, 81% 75 28, respectively. These results indicate that can fields offer a novel insight into significance solar-UV-avoiding application improved pest during sunny summers.

Language: Английский

Citations

9
Rad6, a ubiquitin conjugator required for insect-pathogenic lifestyle, UV damage repair, and genomic expression of Beauveria bassiana DOI

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127622 - 127622

Published: Jan. 18, 2024

Language: Английский

Citations

3
Comparative Roles of Rad4A and Rad4B in Photoprotection of Beauveria bassiana from Solar Ultraviolet Damage DOI Creative Commons
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 154 - 154

Published: Jan. 23, 2023

The Rad4-Rad23-Rad33 complex plays an essential anti-ultraviolet (UV) role depending on nucleotide excision repair (NER) in budding yeast but has been rarely studied filamentous fungi, which possess two Rad4 paralogs (Rad4A/B) and orthologous Rad23 rely the photorepair of UV-induced DNA lesions, a distinct mechanism behind photoreactivation UV-impaired cells. Previously, nucleocytoplasmic shuttling proved to be highly efficient conidia inactivated by UVB, major component solar UV, due its interaction with Phr2 Beauveria bassiana, wide-spectrum insect mycopathogen lacking Rad33. Here, either Rad4A or Rad4B was proven localize exclusively nucleus interact Rad23, previously shown white collar protein WC2 as regulator photorepair-required photolyases (Phr1 Phr2) B. bassiana. Δrad4A mutant lost ~80% conidial UVB resistance ~50% activity UVB-inactivated 5 h light exposure. Intriguingly, reactivation rates UVB-impaired were observable only presence rad4A after dark incubation exceeding 24 h, implicating extant, infeasible, NER for field where night (dark) time is too short. Aside from strong anti-UVB role, played no other bassiana's lifecycle while functionally redundant. Our findings uncover that depends ascribed linked expands molecular basis underlying fungal adaptation UV irradiation Earth's surface.

Language: Английский

Citations

8
Co-Regulatory Roles of WC1 and WC2 in Asexual Development and Photoreactivation of Beauveria bassiana DOI Creative Commons
Siyuan Xu, Lei Yu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 290 - 290

Published: Feb. 23, 2023

The white collar proteins WC1 and WC2 interact with each other to form a complex acting as well-known transcription regulator required for the operation of circadian clock in Neurospora, but their roles insect-pathogenic fungal lifecycles remain poorly understood. Here, we report that orthologs co-regulate conidiation capacity conidial resistance solar ultraviolet-B (UVB) irradiation Beauveria bassiana, after high activities photorepair UVB-induced DNA damages were elucidated previously insect mycopathogen, which features non-rhythmic capacity. yield, UVB resistance, photoreactivation rate UVB-impaired conidia greatly reduced null mutants wc1 wc2 compared control strains. However, many lifecycle-related phenotypes, except antioxidant response, rarely affected two mutants. Transcriptomic analysis revealed largely overlapping regulating gene networks. Most differentially expressed genes identified from (1380) (1001) co-downregulated (536) or co-upregulated (256) at similar levels, including several aerial photorepair. These findings expand molecular basis underlying adaptation UV offer novel insight into genome-wide co-regulatory B. bassiana's asexual development vivo against damage.

Language: Английский

Citations

8
Recovery of insect-pathogenic fungi from solar UV damage: Molecular mechanisms and prospects DOI
Ming‐Guang Feng

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 59 - 82

Published: Jan. 1, 2024

Language: Английский

Citations

2
Rad1 and Rad10 Tied to Photolyase Regulators Protect Insecticidal Fungal Cells from Solar UV Damage by Photoreactivation DOI Creative Commons
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(11), P. 1124 - 1124

Published: Oct. 25, 2022

Beauveria bassiana serves as a main source of global fungal insecticides, which are based on the active ingredient formulated conidia vulnerable to solar ultraviolet (UV) irradiation and restrained for all-weather application in green agriculture. The anti-UV proteins Rad1 Rad10 required nucleotide excision repair (NER) UV-injured DNA model yeast, but their roles remain rarely exploredin filamentous fungi. Here, orthologues that accumulated more nuclei than cytoplasm B. proved capable reactivating UVB-impaired or UVB-inactivated efficiently by 5h light exposure incapable doing so 24 h dark incubation if UVB was lethal. Each orthologue found interacting with other two white collar (WC1 WC2), be regulators photolyases (Phr1 Phr2) individually efficient photorepair UVB-induced lesions either photolyase alone. photoreactivation activity far compromised when protein-protein interactions were abolished absence Phr1 Phr2 lost function. detected suggest direct links regulators. In bassiana, therefore, tied have high activities photoprotection from UV damage insufficient NER field, where night (dark) time is too short, no lifecycle vitro vivo.

Language: Английский

Citations

11
Effect of ultraviolet radiation on Beauveria bassiana virulence and development of protective formulations DOI

Haoran Jia,

Ibrahima Camara,

Zhen Zhang

et al.

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(4)

Published: March 10, 2023

Language: Английский

Citations

6
Mycoinsecticides applied in late afternoon to avoid solar UV damage are more efficacious against rice leaf-rolling insect pests than those applied in the morning DOI

Dan‐Yi Qi,

Wan-Ying Xu,

Yan‐Zhi Shao

et al.

Biological Control, Journal Year: 2023, Volume and Issue: 186, P. 105352 - 105352

Published: Sept. 20, 2023

Language: Английский

Citations

6