Harnessing Non-Antibiotic Strategies to Counter Multidrug-Resistant Clinical Pathogens with Special Reference to Antimicrobial Peptides and Their Coatings

Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 57 - 57

Published: Jan. 9, 2025

Antimicrobial resistance is a critical global challenge in the 21st century, validating Sir Alexander Fleming's warning about misuse of antibiotics leading to resistant microbes. With dwindling arsenal effective antibiotics, it imperative concentrate on alternative antimicrobial strategies. Previous studies have not comprehensively discussed advantages and limitations various strategies, including bacteriophage therapy, probiotics, immunotherapies, photodynamic essential oils, nanoparticles peptides (AMPs) within single review. This review addresses that gap by providing an overview these non-antibiotic highlighting their pros cons, with particular emphasis (AMPs). We explore mechanism action AMPs against bacteria, viruses, fungi parasites. While hold significant promise, application mainstream drug development hindered challenges such as low bioavailability potential toxicity. However, advancements peptide engineering chemical modifications offer solutions enhance clinical utility. Additionally, this presents updates strategies aimed at improving cost, stability selective toxicity through peptidomimetics. These molecules demonstrated activity broad range pathogens, making them valuable candidates for integration into surface coatings prevent device-associated infections. Furthermore, we discuss approaches attaching functionalising surfaces. Finally, recommend comprehensive vivo evaluate efficacy mimetics, investigate synergistic combinations other assess medical devices.

Language: Английский

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Antimicrobial Peptides, Their Production, and Potential in the Fight Against Antibiotic-Resistant Pathogens

Fermentation, Journal Year: 2025, Volume and Issue: 11(1), P. 36 - 36

Published: Jan. 17, 2025

The article reviews the literature on antimicrobial peptides (AMPs) that exhibit unique mechanisms, such as broad-spectrum activity, low development of resistance, and ability to modulate immune response host organism. Information is provided significant potential AMPs in fight against pathogens threatening human health food safety. Enrichment diet with biologically active obtained using proteolytic activity lactic acid bacteria (LAB) proposed a simple, accessible, viable alternative antibiotics does not have harmful side effect. review briefly covers methods for obtaining features LAB system responsible producing bioactive environment. It has been shown various strains makes it possible produce high-quality whey-based beverages different directions antagonistic opportunistic helps optimize gastrointestinal microbiota. assumed drinks can reduce dose antimicrobials combined therapy infectious diseases be preventive measure contagion spread resistance.

Language: Английский

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Pyrrolysine Aminoacyl-tRNA Synthetase as a Tool for Expanding the Genetic Code

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 539 - 539

Published: Jan. 10, 2025

In addition to the 20 canonical amino acids encoded in genetic code, there are two non-canonical ones: selenocysteine and pyrrolysine. The discovery of pyrrolysine synthetases (PylRSs) was a key event field code expansion research. importance this is mainly due fact that translation systems involving PylRS, tRNA (tRNAPyl) orthogonal endogenous organisms do not use acid protein synthesis. addition, belonging different groups also mutually orthogonal. This orthogonality based on structural features PylRS tRNAPyl, which include identical elements, such as condensed core, certain base pairs motifs tRNAPyl. suggests targeted changes these molecules enable their specificity for codon. Such modifications were successfully used obtain aaRS/tRNA allow incorporation unnatural into peptides. review presents results recent studies related correlation between structure activity tRNAPyl extend code.

Language: Английский

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FDA-Approved Antibacterials and Echinocandins

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 166 - 166

Published: Feb. 7, 2025

Since 1955, a total of 12 peptide-based drugs with antimicrobial or antifungal properties have received approval from the Food and Drug Administration (FDA). Peptides present promising opportunity to address serious infections that may be challenging manage through other means. exhibit capability leverage various mechanisms, in some cases, multiple mechanisms are employed for this purpose. Despite initial dating back FDA recently approved an echinocandin peptide just last year. The ongoing approvals underscore significance peptides addressing medical challenges. Approximately 22 therapeutics antibacterial spectrum currently undergoing phases clinical trials, showing results. In review, analyzed terms their chemical structure, indication, mode action, development journey, concluding arrival pharmaceutical market.

Language: Английский

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Screening of Short-Chain Antimicrobial Peptide LKARI with Broad-Spectrum Bactericidal Properties and Its Application in Promoting Wound Healing

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(25), P. 32087 - 32103

Published: June 12, 2024

Due to the extensive use of antibiotics, many highly resistant bacteria and extensively have been produced. In recent years, increase drug-resistant resulting proliferation increased incidence hospital-acquired infections caused great harm human health. Antimicrobial peptides (AMPs) are considered be an innovative antibiotic belong latest advances in this field. We designed a polypeptide verified its low minimum inhibitory concentration broad-spectrum activity against Gram-positive bacteria, Gram-negative fungi microbiology pharmacology. Several experiments confirmed that screened antimicrobial significant antidrug resistance also show therapeutic properties treatment systemic bacterial infections. addition, through our experimental research, it was proved antibacterial hydrogel composed poly(vinyl alcohol), sodium alginate, had excellent showed good wound healing ability.

Language: Английский

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Exploring the Efficacy of Peptides and Mimics against Influenza A Virus, Adenovirus, and Murine Norovirus

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7030 - 7030

Published: June 27, 2024

The ongoing battle against viral pandemics continues, with the possibility of future outbreaks. search for effective antiviral compounds that can combat a diverse range viruses continues to be focal point research. This study investigated efficacy two natural antimicrobial peptides (AMPs) (lactoferricin and LL-37), synthetic AMPs (melimine Mel4), nine AMP mimics (758, 1091, 1096, 1083, 610, NAPL, 3-BIPL, 4-BIPL, Sau-22) influenza A virus strains H1N1 H3N2, human adenovirus 5 (HAdV-5), murine norovirus 1 (MNV-1). These were tested using pre-treatment, cell or post-cell entry treatment assays, electron microscopy, circular dichroism (CD), alongside evaluations cytotoxicity host cells. After 610 Sau-22 had relatively low IC50 values (2.35 6.93 µM, respectively) H3N2 (3.7 5.34 respectively). Conversely, not active these strains. For non-enveloped viruses, Mel4 mimic 1083 moderate activity HAdV-5 (Mel4 = 47.4 µM; 47.2 µM), whereas all AMPs, but none mimics, (LL-37 4.2 lactoferricin 23.18 melimine 4.8 8.6 µM). Transmission microscopy demonstrated targeted outer envelope while capsid viruses. CD showed adopted an α-helical structure in membrane mimetic environment, 758 remained unstructured. different groups is probably influenced by charge, hydrophobicity, size, and, case their secondary structure. findings underscore potential as promising candidates therapeutics both enveloped

Language: Английский

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Human microbiome derived synthetic antimicrobial peptides with activity against Gram-negative, Gram-positive, and antibiotic resistant bacteria

RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

An AMP-derived short 15-mer peptide and its cyclic derivatives have low micromolar broad spectrum antibacterial activity, with rapid onset of bactericidal effect a membrane-targeting mode action.

Language: Английский

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The Promising Role of Amine Transaminase Cascades in the Synthesis of Non-Canonical Amino Acids

Processes, Journal Year: 2024, Volume and Issue: 12(11), P. 2566 - 2566

Published: Nov. 16, 2024

Amine transaminases (ATA) are critical players in producing non-canonical amino acids, essential building blocks pharmaceuticals and fine chemicals. Significant progress has been made discovering engineering enzymes this field, enhancing their use organic synthesis. However, challenges such as co-factor regeneration, substrate, product inhibition remain significant limitations to widespread industrial enzyme application. (Chemo-)enzymatic cascades offer efficient environmentally friendly pathways for synthesizing reducing the need multiple synthesis steps saving purification of intermediates. This review focuses specifically on emphasizing enzymatic chemoenzymatic involving ATA.

Language: Английский

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Peptides as Alternatives to Antibiotics in Poultry Health Management

Published: Jan. 1, 2024

Language: Английский

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