Fabrication and in vitro/vivo evaluation of quercetin nanocrystals stabilized by glycyrrhizic acid for liver targeted drug delivery DOI Creative Commons
Baode Shen,

Yuwen Zhu,

Fengxia Wang

et al.

International Journal of Pharmaceutics X, Journal Year: 2024, Volume and Issue: 7, P. 100246 - 100246

Published: April 9, 2024

The purpose of this study was to design novel drug nanocrystals (NCs) stabilized by glycyrrhizic acid (GL) for achieving liver targeted delivery due the presence GL receptor in hepatocytes. Quercetin (QT) exhibits good pharmacological activities treatment diseases, including steatosis, fatty hepatitis, fibrosis, and cancer. It selected as a model owing its poor water solubility. QT NCs (QT-NCs/GL) were fabricated wet media milling technique systemically evaluated. QT-NCs poloxamer 188 (QT-NCs/P188) prepared reference comparison vitro vivo performance with QT-NCs/GL. QT-NCs/GL QT-NCs/P188 similar particle size around 130 nm successfully technique. Both showed irregular particles short rods under SEM. XRPD revealed that remained crystalline state reduced crystallinity. exhibited significant solubility increase release improvement compared raw QT. No difference plasma concentration–time curves pharmacokinetic parameters found following intravenous administration QT-NCs/P188. However, significantly higher distribution observed QT-NCs/P188, indicating could achieve be concluded used stabilizer have great potential delivery.

Language: Английский

Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression DOI
Qing Zhu,

Yuanshan Han,

Ying He

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155560 - 155560

Published: April 4, 2024

Language: Английский

Citations

12
Quercetin alleviates LPS/iE-DAP-induced liver injury by suppressing ferroptosis via regulating ferritinophagy and intracellular iron efflux DOI Creative Commons

Hongzhu Zhang,

Huimin Shi, Xuerui Li

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: 81, P. 103557 - 103557

Published: Feb. 18, 2025

Ruminal dysbiosis-induced liver injury is prevalent in dairy cows, yet its underlying mechanisms remain incompletely understood. Ferroptosis, a newly identified form of programmed cell death distinct from apoptosis and necrosis, has been implicated various diseases by emerging studies. In the present study, lipopolysaccharide (LPS) γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) were employed to establish vitro vivo models using bovine hepatocytes mice, respectively. It was observed that noncytotoxic iE-DAP alone did not influence lipid peroxidation or GPX4, but exacerbated LPS-induced ferroptosis hepatocyte injury. Notably, co-treatment with LPS (LPS/iE-DAP)-induced mitigated intervention inhibitor ferrostatin-1 (Fer-1). Mechanistically, activated IL-6/STAT3 signaling pathway found mediate LPS/iE-DAP-induced ferroptosis. Suppression IL-6/STAT3, either through IL6 STAT3 knockdown pharmacological intervention, reduced Fe2+ accumulation alleviated ferroptotic death. Further investigations enhanced ferritinophagy impaired iron export. Either disrupting knocking down NCOA4 restoring export via HAMP relieved intracellular overload inhibited Specifically, LPS/iE-DAP treatment increased interaction between hepcidin ferroportin, promoting ferroportin ubiquitination degradation, thereby blocking efflux. Furthermore, we provided several evidence prove quercetin pretreatment decreasing hepatic targeting vivo, effects reversed addition recombinant IL-6. Based on these findings, concluded triggering regulating IL-6/STAT3/ferritinophagy-dependent release IL-6/STAT3/hepcidin/ferroportin-dependent export, while could alleviate this inhibiting pathway. This study provides novel insights into whereby ruminal dysbiosis induces presents prospective solution for

Language: Английский

Citations

1
Lonicera japonica polysaccharides alleviate D-galactose-induced oxidative stress and restore gut microbiota in ICR mice DOI

Wenwen Sun,

Jiahao Zhu,

Guanyu Qin

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 245, P. 125517 - 125517

Published: June 22, 2023

Language: Английский

Citations

22
Challenges and opportunities for improving the druggability of natural product: Why need drug delivery system? DOI Creative Commons
Peng Tang, Tianze Shen, Hairong Wang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 164, P. 114955 - 114955

Published: June 1, 2023

Bioactive natural products (BNPs) are the marrow of medicinal plants, which secondary metabolites organisms and have been most famous drug discovery database. for their enormous number great safety in medical applications. However, BNPs troubled by poor druggability compared with synthesis drugs challenged as medicine (only a few applied clinical settings). In order to find reasonable solution improving BNPs, this review summarizes bioactive nature based on pharmacological research tries explain reasons BNPs. And then focused boosting loaded delivery systems, further concludes advantages systems improvement from perspective nature, discusses why need predicts next direction.

Language: Английский

Citations

19
Research progress of sorafenib drug delivery system in the treatment of hepatocellular carcinoma: An update DOI Open Access

Qiang-qiang Fan,

Huan Tian,

Jiangxue Cheng

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 117118 - 117118

Published: July 14, 2024

Hepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors in contemporary era, representing a significant global health concern. Early HCC patients have mild symptoms or are asymptomatic, which promotes onset and progression disease. Moreover, advanced insensitive to chemotherapy, making traditional clinical treatment unable block cancer development. Sorafenib (SFB) first-line targeted drug for with anti-angiogenesis anti-tumor cell proliferation effects. However, efficacy SFB constrained by its off-target distribution, rapid metabolism, multi-drug resistance. In recent years, nanoparticles based on variety materials been demonstrated enhance targeting therapeutic against HCC. Concurrently, advent joint delivery systems has furnished crucial empirical evidence reversing This review will summarize application nanotechnology field over past five years. It focus research progress combined multiple modalities treatment.

Language: Английский

Citations

6
Moringa oleifera Leaf Extract Protects C2C12 Myotubes against H2O2-Induced Oxidative Stress DOI Creative Commons
Roberta Ceci, Mariateresa Maldini, Mark E. Olson

et al.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(8), P. 1435 - 1435

Published: July 24, 2022

The imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule tissue damage as a result of cellular oxidative stress. This phenomenon is considered key factor in fatigue muscle following chronic or high-intensity physical exercise. In the present study, effect Moringa oleifera leaf extract (MOLE) was evaluated C2C12 myotubes exposed an elevated hydrogen peroxide (H2O2) insult. capacity influence myotube redox status through analysis total (TAC), glutathione homeostasis (GSH GSSG), free thiols (TFT), thioredoxin (Trx) activity, well enzyme activities superoxide dismutase (SOD), catalase (CAT), peroxidase (GPx) transferase (GST). Moreover, ability MOLE mitigate stress-induced peroxidation lipids (TBARS protein carbonyls) also evaluated. Our data demonstrate that pre-treatment mitigates highly stressful effects H2O2 (1 mM) by restoring (TFT, Trx, GSH/GSSG ratio) increasing enzymatic system (CAT, SOD, GPx, GST), thereby significantly reducing TBARs PrCAR levels. study provides evidence supplementation has potential, allowing better able cope with insult and, therefore, could represent useful nutritional strategy for preservation well-being.

Language: Английский

Citations

27
Potential Therapeutic Implication of Herbal Medicine in Mitochondria-Mediated Oxidative Stress-Related Liver Diseases DOI Creative Commons
Moon Nyeo Park, Md. Ataur Rahman, MD. Hasanur Rahman

et al.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(10), P. 2041 - 2041

Published: Oct. 17, 2022

Mitochondria are double-membrane organelles that play a role in ATP synthesis, calcium homeostasis, oxidation-reduction status, apoptosis, and inflammation. Several human disorders have been linked to mitochondrial dysfunction. It has found traditional therapeutic herbs effective on alcoholic liver disease (ALD) nonalcoholic fatty (NAFLD) which leading causes of cirrhosis hepatocellular carcinoma. The generation reactive oxygen species (ROS) response oxidative stress is caused by dysfunction considered critical for treatment. stress, lipid toxicity, inflammation NAFLD well known. chronic commonly progresses disease, people with obesity, insulin resistance, diabetes, hyperlipidemia, hypertension at higher risk developing NAFLD. associated number pathological factors, including metabolic dysfunction, inflammation, fibrosis. As result, the improvement steatosis enough entice researchers look into However, antioxidant treatment not very disease. Additionally, it suggested beneficial effects herbal medicines immunity governed various mechanisms metabolism control. This review provided summary research implementation mitochondria-mediated ROS production as clinical applications through medicine. In addition, pathophysiology common such ALD would be investigated mitochondria process open new avenues management patients

Language: Английский

Citations

23
Modulation of NRF2: Biological Dualism in Cancer, Targets and Possible Therapeutic Applications DOI
M. Gallorini, Simone Carradori, Emiliano Panieri

et al.

Antioxidants and Redox Signaling, Journal Year: 2023, Volume and Issue: 40(10-12), P. 636 - 662

Published: July 20, 2023

The nuclear factor erythroid 2-related 2 (NRF2)-Kelch-like ECH-associated protein 1 (KEAP1) system is a master regulator of redox homeostasis and cell adaptation to variety exogenous endogenous stressors. Accumulating evidence from the last decade indicates that impairment balance leads oxidative stress (OS), common alteration occurring in many human acute chronic inflammatory diseases, such as cancer, diabetes, neurodegeneration, metabolic disorders, aging.

Language: Английский

Citations

16
Quercetin/Anti-PD-1 Antibody Combination Therapy Regulates the Gut Microbiota, Impacts Macrophage Immunity and Reshapes the Hepatocellular Carcinoma Tumor Microenvironment DOI Creative Commons
Ruoxia Wu, Jiaqing Xiong, Ting Zhou

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2023, Volume and Issue: 28(12), P. 327 - 327

Published: Dec. 1, 2023

Objective: The use of immune checkpoint inhibitors (ICIs) provides promising strategies for hepatocellular carcinoma (HCC) treatment. This study aimed to explore impact and underlying mechanism the combination therapy quercetin anti-programmed cell death 1 (anti-PD-1) antibody on HCC. Methods: Orthotopically transplanted HCC tumors in mice were treated with quercetin, anti-PD-1 antibody, or a both therapies. Histopathological changes programmed ligand (PD-L1) expression characterized by hematoxylin eosin (H&E) immunohistochemistry (IHC) staining. diversity differences gut microbiota (GM) evaluated through 16S rRNA sequencing. Levels macrophage immunity-related cytokines quantified enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (RT-qPCR), Western blot. Results: Combination reduced necrosis, fibrosis, PD-L1 liver tissues. Additionally, GM imbalance increased abundance Firmicutes, Actinobacteria, Verrucomicrobiota at phylum level as well Dubosiella Akkermansia genus level. improved immunity, raised expressions CD8a, CD4, CD11b, interleukin (IL)-10, interferon (IFN)-γ , declined IL-4, IL-6, toll-like receptor 4 (TLR4), an inhibitor nuclear factor κBα (IκBα), NFκB subunit p65. Upon therapy, M2 macrophage-related genes arginase-1 (Arg-1), IL-10, transforming growth factor-β (TGF-β), matrix metalloproteinase-9 (MMP-9) upregulated. Instead, M1 IL-12a, IL-1β, tumor necrosis factor-α (TNF-α) downregulated. Conclusions: Quercetin/anti-PD-1 reshaped microenvironment parallel regulating immunity.

Language: Английский

Citations

15
Lipoic acid-based poly(disulfide)s: Synthesis and biomedical applications DOI Creative Commons
Ivan O. Levkovskyi, Shota Mochizuki,

Ajay Zheng

et al.

Nano TransMed, Journal Year: 2023, Volume and Issue: 2(2-3), P. 100006 - 100006

Published: Sept. 1, 2023

Biodegradable and adaptable polymeric materials are currently being studied due to their wide scope of potential applications, from nanomedicine novel multifunctional materials. One such class polymers poly(disulfide)s, which contain repeating disulfide bonds in main chain. Lipoic acid, or thioctic is a biologically derived small molecule containing 1,2-dithiolane ring capable undergoing opening polymerization yield poly(disulfide)s. In this review, we highlight the synthesis lipoic acid-based poly(disulfide)s through thermal thiolate-initiated polymerizations, development methodology pertaining synthetic methods. We further discuss biomedical applications have been widely used construct various responsive biomaterials, including polymer-drug conjugates, nanoparticles, hydrogels, adhesives.

Language: Английский

Citations

13