The Inhibitory Effects and Cytotoxic Activities of the Stem Extract of Nepenthes miranda against Single-Stranded DNA-Binding Protein and Oral Carcinoma Cells

Plants, Journal Year: 2023, Volume and Issue: 12(11), P. 2188 - 2188

Published: May 31, 2023

The carnivorous pitcher plants of the genus Nepenthes exhibit many ethnobotanical uses, including treatments stomachache and fever. In this study, we prepared different extracts from pitcher, stem, leaf miranda obtained using 100% methanol analyzed their inhibitory effects on recombinant single-stranded DNA-binding protein (SSB) Klebsiella pneumoniae (KpSSB). SSB is essential for DNA replication cell survival thus an attractive target potential antipathogen chemotherapy. Different Sinningia bullata, a tuberous member flowering plant family Gesneriaceae, were also used to investigate anti-KpSSB properties. Among these extracts, stem extract N. exhibited highest activity with IC50 value 15.0 ± 1.8 μg/mL. cytotoxic apoptosis cancer lines Ca9-22 gingival carcinoma, CAL27 oral adenosquamous PC-9 pulmonary adenocarcinoma, B16F10 melanoma, 4T1 mammary carcinoma cells demonstrated compared. Based collective data, activities at concentration 20 μg/mL followed order > PC9 cells. 40 completely inhibited migration proliferation. addition, incubation boosted distribution G2 phase 7.9% 29.2% in cells; other words, might suppress proliferation by inducing cycle arrest. Through gas chromatography-mass spectrometry, 16 most abundant compounds tentatively identified. 10 docking analysis, scores binding capacity was sitosterol hexadecanoic acid oleic plumbagin 2-ethyl-3-methylnaphtho[2,3-b]thiophene-4,9-dione methyl α-d-galactopyranoside 3-methoxycatechol catechol pyrogallol hydroxyhydroquinone; thus, greatest against KpSSB among selected compounds. Overall, results may indicate pharmacological further therapeutic applications.

Language: Английский

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Phytosterols activating nuclear receptors are involving in steroid hormone-dependent cancers: Myth or fact?

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 169, P. 115783 - 115783

Published: Nov. 7, 2023

Nuclear receptors (NRs) represent intracellular proteins that function as a signaling network of transcriptional factors to control genes in response variety environmental, dietary, and hormonal stimulations or serve orphan lacking recognized ligand. They also play an essential role normal development, metabolism, cell growth, division, physiology, reproduction, homeostasis biological markers for tumor subclassification targets hormone therapy. NRs, including steroid (SHRs), have been studied tools examine the fundamentals regulation within development mammals human addition their links disturbances. In this regard, it is widely aberrant NR responsible pathological growth hormone-dependent tumors SHRs dysregulation consequently represents potential therapeutic candidate range diseases, case prostate cancer breast cancer. On other hand, phytosterols are group plant-derived compounds act directly ligands NRs proven efficacy management diabetes, heart cancers. However, these plants not suggested cases since certain contains molecules with chemical structure similar estrogens, which known phytoestrogens estrogen-like compounds, such lignans, coumestans, isoflavones. Therefore, remains open controversial debate regarding whether consuming phytosterol-rich diet adopting vegetarian lifestyle like Mediterranean may increase risk developing cancers by constitutively activating thereby leading transformation. Overall, purpose review better understand relevant mechanistic pathways explore epidemiological investigations order establish contribute activation drivers

Language: Английский

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Antiproliferative Effect of 7- Ketositosterol in Breast and Liver Cancer Cells: Possible Impact of Ceramide, ERK and NF-kB Signaling Pathways

Published: May 28, 2024

Background: This study aimed to examine the effect of 7-Ketositosterol (7-KSS), on sphingomyelin/ceramide metabolites and apoptosis in human breast MCF-7 liver HepG2 cancer cells. Methods: Anti-proliferative effects 7-KSS treatment were assessed at different concentrations periods. Cell viability was through MTT analysis, whereas levels sphingosine-1-phosphate (S1P), sphingomyelins (SMs), ceramides (CERs) measured using LC-MS/MS. Phosphorylated 44/42 ERK1/2 NF-kB p65 (Ser536) protein by western blot analysis immunofluorescence staining. Apoptosis evaluated TUNEL staining flow cytometric assessment annexin-V propidium iodide (PI) labeling. Results: Treatment with significantly decreased cell survival, S1P, p-44/42 ERK1/2, p-NF-κBp65 cells compared controls. A substantial rise detected intracellular amounts C16-C24 CERs incubated 7-KSS. Conclusions: stimulated ceramide accumulation while decreasing proliferation via downregulating levels.

Language: Английский

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Antiproliferative Effect of 7-Ketositosterol in Breast and Liver Cancer Cells: Possible Impact on Ceramide, Extracellular Signal-Regulated Kinases, and Nuclear Factor Kappa B Signaling Pathways

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(7), P. 860 - 860

Published: July 1, 2024

Background: This study aimed to examine the effect of 7-Ketositosterol (7-KSS), on sphingomyelin/ceramide metabolites and apoptosis in human breast MCF-7 liver HepG2 cancer cells. Methods: Anti-proliferative effects 7-KSS treatment were assessed at different concentrations periods. Cell viability was through MTT analysis, whereas levels sphingosine-1-phosphate (S1P), sphingomyelins (SMs), ceramides (CERs) measured using LC-MS/MS. Phosphorylated 44/42 ERK1/2 NF-κB p65 (Ser536) protein by Western blot analysis immunofluorescence staining. Apoptosis evaluated TUNEL staining flow cytometric assessment annexin-V propidium iodide (PI) labeling. Results: Treatment with significantly decreased cell survival S1P, p-44/42 ERK1/2, p-NF-κB cells compared controls. A substantial rise detected intracellular amounts C16-C24 CERs incubated 7-KSS. Conclusions: stimulated ceramide accumulation while decreasing proliferation via downregulating levels.

Language: Английский

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Unveiling the potential of medicinal herbs as the source for in vitro screening toward the inhibition of Nrf2

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38411 - e38411

Published: Sept. 26, 2024

Language: Английский

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Efficacy of Ergosterol and Ergosterol peroxide in different anti-inflammatory models – a comparison study

Food and Agricultural Immunology, Journal Year: 2024, Volume and Issue: 35(1)

Published: Nov. 24, 2024

Ergosterol and its peroxide with important regulatory roles in a variety of intracellular biological processes. In this study, both the LPS- UVA-induced human keratinocyte (HaCaT) inflammatory models were established to investigate anti-inflammatory effects ES EP. The protective EP on UVA LPS-induced cell damage discussed by viability assays, reactive oxygen species levels, skin barrier-related proteins, factors, expression genes involved related signaling pathways analyzed. results showed that could reduce ROS levels; inhibit IL-8 IL-1β secretion; had significant promotion AQP3 FLG, inhibited gene expressions PI3K, AKt, not irritating according safety evaluation. summary, excellent provided degree protection against UVA/LPS-induced damage.

Language: Английский

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Current and New Insights on Delivery Systems for Plant Sterols in Food

Advances in experimental medicine and biology, Journal Year: 2023, Volume and Issue: unknown, P. 403 - 435

Published: Dec. 1, 2023

Language: Английский

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Anti-Skin Aging Potential, Antibacterial Activity, Inhibition of Single-Stranded DNA-Binding Protein, and Cytotoxic Effects of Acetone-Extracted Passiflora edulis (Tainung No. 1) Rind Extract on Oral Carcinoma Cells

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2194 - 2194

Published: Aug. 8, 2024

The passion fruit,

Language: Английский

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Unlocking the Insightful Antidiabetic Effects of Lemongrass (Cymbopogon citratus): A Comprehensive Review

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown, P. 90 - 104

Published: Aug. 19, 2024

Lemongrass (Cymbopogon citratus) has emerged as a noteworthy contender in traditional medicine for its aromatic and therapeutic attributes, yet potential an antidiabetic agent remains largely unexplored the literature. To address this gap, study endeavors to provide comprehensive overview of lemongrass role diabetes management underlying pathways involved. Rich bioactive compounds such citral, flavonoids, essential oils, boasts diverse array medicinal properties. Research indicates capability modulate blood glucose levels, improve insulin sensitivity, fortify pancreatic β-cell function primarily through antioxidant anti-inflammatory mechanisms. Animal studies have demonstrated substantial reductions fasting enhanced tolerance, ascribed heightened signaling inhibition carbohydrate metabolism enzymes. Additionally, exhibits positive impact on lipid profiles, thereby mitigating cardiovascular risks associated with diabetes. Limited human trials corroborated these findings, showcasing improvements glycemic control following consumption. In conclusion, emerges promising adjunctive therapy management, owing natural composition multifaceted Nonetheless, further investigation, particularly rigorous clinical trials, is imperative determine optimal dosages, long-term safety, efficacy, facilitating seamless integration into conventional treatment protocols.

Language: Английский

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Bee Pollen as a Source of Biopharmaceuticals for Neurodegeneration and Cancer Research: A Scoping Review and Translational Prospects

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5893 - 5893

Published: Dec. 13, 2024

Bee Pollen (BP) has many advantageous properties relying on its multitargeting potential, a new tendency in managing challenging illnesses. In cancer and neurodegeneration, the multiple effects of BP could be unequaled importance need further investigation. Although still limited, available data interestingly spotlights some floral sources with promising activities line this Adopting scoping review methodology, we have identified crucial bioactivities that are widely recognized to individual compounds but remain completely untapped valuable bee cocktail. A wide range these been recently found endowed great potential modulating pivotal processes neurodegeneration pathophysiology. addition, ubiquitous only isolated, while number studied BPs remains extremely limited compared endless pool plant species worldwide. We also elucidated clinical profits from perspectives impeded by hurdles such as bioavailability phytocompounds, diversity lack phytochemical standardization BP, difficulty selective targeting pathophysiological mechanisms. finally present interesting insights guide future research pave way for urgently needed simplified investigations.

Language: Английский

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