Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
135(51)
Published: Nov. 1, 2023
Abstract
The
glutathione
(GSH)
system
is
one
of
the
most
powerful
intracellular
antioxidant
systems
for
elimination
reactive
oxygen
species
(ROS)
and
maintaining
cellular
redox
homeostasis.
However,
rapid
kinetics
information
(at
millisecond
to
second
level)
during
dynamic
antioxidation
process
GSH
remains
unclear.
As
such,
we
specifically
developed
a
novel
dual‐wire
nanosensor
(DWNS)
that
can
selectively
synchronously
measure
levels
ROS
with
high
temporal
resolution,
applied
it
monitor
transient
generation
as
well
in
individual
cancer
cells.
These
measurements
revealed
peroxidase
(GPx)
rapidly
initiated
against
burst
sub‐second
time
scale,
but
short‐lived,
ending
after
few
seconds,
while
some
are
still
present
This
study
expected
open
new
perspectives
understanding
studying
imbalance‐related
physiological.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 11, 2024
Abstract
Resveratrol
(RSV)
is
a
plant-derived
natural
product
with
diverse
biological
activities.
It
has
attracted
considerable
attention
for
its
notable
efficacy
in
nutritional
health
and
disease
treatment.
The
physiological
impact
of
RSV
the
human
body
closely
connected
to
gut
microbiota;
however,
primary
microorganisms
responsible
metabolism
underlying
mechanisms
remain
unclear.
Here,
based
on
ex
vivo
culturing
microbiota
from
feces,
we
isolated
bacterium
capable
efficiently
metabolizing
RSV,
namely
Eggerthella
lenta
J01.
Through
induced
enrichment
transcriptomics
bioinformatic
analyses,
further
identified
resveratrol
reductase
(RER)
E.
J01,
which
specifically
catalyzes
hydrogenation
C9–C10
double
bond
initiates
RSV’s
metabolism.
RER
homologs
represent
novel
class
ene-reductases.
abundance
healthy
individuals
was
significantly
higher
than
that
patients
inflammatory
bowel
disease,
suggesting
crucial
function.
Cell
culture
experiments
animal
studies
showed
dihydroresveratrol
(DHR),
metabolite
catalyzed
by
RER,
exhibited
stronger
activity
inhibiting
growth
colon
cancer
cells
alleviating
symptoms
enteritis
mouse
models.
Our
results
expand
understanding
microbial
medicinal
functions,
providing
possible
guidance
optimizing
bioavailability
body.
Cell Death and Disease,
Journal Year:
2024,
Volume and Issue:
15(11)
Published: Nov. 5, 2024
Abstract
Dysfunction
of
zinc
finger
protein
652
(ZNF652)
is
associated
with
various
malignant
tumors.
However,
the
role
ZNF652
in
lung
cancer
(LC)
poorly
understood.
Here,
we
identified
that
was
downregulated
human
LC
tissues
and
cell
lines.
Low
expression
poor
survival
patients.
Overexpression
inhibited
viability,
proliferation,
migration,
invasion
cells,
whereas
knockdown
promoted
these
phenotypes.
Using
RNA-seq
analysis
revealed
overexpression
resulted
obvious
alterations
biological
processes,
especially
cycle
cellular
senescence.
Subsequently,
confirmed
arrested
at
G1
phase,
increased
ROS-mediated
DNA
damage,
induced
senescence,
enhanced
cisplatin-induced
apoptosis
cells.
Mechanistically,
directly
bound
to
promoter
cyclin
D3
(CCND3),
its
transcription,
thereby
arresting
phase.
Ectopic
rescued
decreased
viability
arrest
by
ZNF652.
In
vivo
studies
further
showed
suppressed
tumorigenic
potential
LC.
Collectively,
our
findings
reveal
exerts
a
tumor
suppressor
inducing
senescence
via
transcriptionally
downregulating
D3.
Thus,
may
be
prognostic
predictive
factor
for
Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
16(12), P. 1588 - 1588
Published: Dec. 12, 2024
Background:
Resveratrol
(RSV)
is
a
natural
polyphenol
that
offers
antioxidant,
anti-inflammatory,
and
chemopreventive
benefits.
This
project
determined
the
ability
of
RSV-loaded
nanoparticles
(NP)
to
inhibit
growth
lung
tumor
spheroids
in
vitro.
Methods:
RSV
was
encapsulated
NP
comprised
biodegradable
polymer,
acetalated
dextran.
A549
cancer
cells
two-dimensional
three-dimensional
cell
culture
models
were
exposed
free
evaluate
their
effect
on
proliferation
spheroid
formation
growth.
For
prevention
studies,
day
0,
for
treatment
dosed
with
same
formulations
5
after
had
fully
formed.
Results:
The
resulting
200
nm
diameter
neutral
surface
charge
exhibited
control
release
vitro
based
environmental
pH.
In
comparison
RSV,
exerted
greater
inhibitory
spheroids.
Conclusions:
have
potential
be
used
as
agent
cancer.
MedComm – Oncology,
Journal Year:
2024,
Volume and Issue:
3(4)
Published: Dec. 1, 2024
Abstract
Oxidative
stress
results
from
an
imbalance
between
the
production
and
neutralization
of
reactive
oxygen
species.
It
induces
oxidative
damage
to
cellular
components
including
proteins,
lipids,
nucleic
acids,
membranes,
therefore
intrinsically
linking
aging‐related
diseases
such
as
cancer,
cardiovascular
disease,
neurological
disorders.
Emerging
evidence
suggests
that
may
promote
tumor
development
by
influencing
various
aspects
senescence,
its
onset,
pro‐inflammatory
secretion,
alteration
function
structure.
Modulating
target
senescence
offers
a
novel
strategy
for
cancer
prevention
treatment.
However,
thorough
grasp
specific
mechanisms
at
play
is
lacking.
This
review
will
present
association
their
regulatory
role
in
progression
treatment,
with
emphasis
on
senescence‐associated
secretory
phenotype,
immunosenescence
therapy‐induced
senescence.
Current
agents
strategies
remove
side
effects
via
killing
senescent
cells
or
modulating
improve
antitumor
efficacy
be
summarized.
help
readers
better
understand
complex
relationship
also
provide
basis
further
research
this
area.
Cell Biology International,
Journal Year:
2023,
Volume and Issue:
47(8), P. 1441 - 1452
Published: May 3, 2023
An
elevation
of
pathologic
intraocular
pressure
(IOP)
is
the
greatest
risk
factor
for
glaucoma.
CD154
has
been
reported
to
bind
CD40
expressed
by
orbital
fibroblasts
and
be
involved
in
immune
inflammatory
responses.
However,
function
mechanism
ocular
hypertensive
glaucoma
(OHG)
are
not
fully
understood.
We
isolated
characterized
Müller
cells
subsequently
examined
effect
on
ATP
release
from
those
cells.
After
being
cocultured
with
CD154-pretreated
cells,
retinal
ganglion
(RGCs)
were
treated
P2X7
siRNAs
or
a
inhibitor.
Furthermore,
mouse
models
(GC)
injected
shRNA.
p21,
p53,
expression
examined,
cellular
senescence
apoptosis
detected
β-Gal
TUNEL
staining,
pathology
was
H&E
ELISA.
induced
accelerated
RGCs
that
had
also
found
treatment
could
attenuate
mediated
pretreated
CD154.
In
vivo
studies
GC
model
mice
verified
silencing
attenuated
pathological
damage
prevented
tissue.
The
study
demonstrates
how
accelerates
aging
co-cultivating
OHG.
research
implies
potential
become
new
therapeutic
target
hypertension
glaucoma,
providing
direction
its
treatment.
Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
135(51)
Published: Nov. 1, 2023
Abstract
The
glutathione
(GSH)
system
is
one
of
the
most
powerful
intracellular
antioxidant
systems
for
elimination
reactive
oxygen
species
(ROS)
and
maintaining
cellular
redox
homeostasis.
However,
rapid
kinetics
information
(at
millisecond
to
second
level)
during
dynamic
antioxidation
process
GSH
remains
unclear.
As
such,
we
specifically
developed
a
novel
dual‐wire
nanosensor
(DWNS)
that
can
selectively
synchronously
measure
levels
ROS
with
high
temporal
resolution,
applied
it
monitor
transient
generation
as
well
in
individual
cancer
cells.
These
measurements
revealed
peroxidase
(GPx)
rapidly
initiated
against
burst
sub‐second
time
scale,
but
short‐lived,
ending
after
few
seconds,
while
some
are
still
present
This
study
expected
open
new
perspectives
understanding
studying
imbalance‐related
physiological.
