DNA origami

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Jan. 28, 2021

Language: Английский

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Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(7), P. 3976 - 4050

Published: March 29, 2023

DNA nanotechnology is a unique field, where physics, chemistry, biology, mathematics, engineering, and materials science can elegantly converge. Since the original proposal of Nadrian Seeman, significant advances have been achieved in past four decades. During this glory time, origami technique developed by Paul Rothemund further pushed field forward with vigorous momentum, fostering plethora concepts, models, methodologies, applications that were not thought before. This review focuses on recent progress origami-engineered nanomaterials five years, outlining exciting achievements as well unexplored research avenues. We believe spirit assets Seeman left for scientists will continue to bring interdisciplinary innovations useful next decade.

Language: Английский

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SERS sensing for cancer biomarker: Approaches and directions

Bioactive Materials, Journal Year: 2023, Volume and Issue: 34, P. 248 - 268

Published: Dec. 31, 2023

These days, cancer is thought to be more than just one illness, with several complex subtypes that require different screening approaches. can distinguished by the distinct markings left metabolites, proteins, miRNA, and DNA. Personalized illness management may possible if categorized according its biomarkers. In order stop from spreading posing a significant risk patient survival, early detection prompt treatment are essential. Traditional techniques tedious, time-consuming, expert personnel for analysis. This has led scientists reevaluate methodologies make use of emerging technologies achieve better results. Using time money saving techniques, these integrate procedures sample preparation in small devices high accuracy sensitivity. With proven potential biomedical use, surface-enhanced Raman scattering (SERS) been widely used biosensing applications, particularly biomarker identification. Consideration was given especially SERS as portable clinical diagnostic tool. The approaches SERS-based sensing both invasive non-invasive samples reviewed this article, along obstacles. Aside constraints approach review also takes into account complexity biological fluids, availability biomarkers, their sensitivity selectivity, which generally lowered. Massive ways maintain capabilities being developed recently get over restriction. known reliable method judgments. Nonetheless, there still room advancement terms portability, creation apps, interdisciplinary AI-based applications. Therefore, we will outline current state technological maturity article. meet demand reviewing various types (invasive non-invasive) biomarkers using SERS. It shed light on growing body research methods application quick detection.

Language: Английский

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Robotic DNA Nanostructures

ACS Synthetic Biology, Journal Year: 2020, Volume and Issue: 9(8), P. 1923 - 1940

Published: June 26, 2020

Over the past decade, DNA nanotechnology has spawned a broad variety of functional nanostructures tailored toward enabled state at which applications are coming increasingly in view. One branches these is synthetic biology, where intrinsic programmability may pave way for smart task-specific molecular robotics. In brief, synthesis user-defined artificial nano-objects based on employing molecules with custom lengths and sequences as building materials that predictably assemble together by obeying Watson–Crick base pairing rules. The general workflow creating nanoshapes getting more straightforward, some objects can be designed automatically from top down. versatile serve tools interface example, therapeutics diagnostics dynamic logic-gated nanopills, light-, pH-, thermally driven devices. Such diverse apparatuses also optical polarizers, sensors capsules, autonomous cargo-sorting robots, rotary machines, precision measurement tools, well electric magnetic-field directed robotic arms. this review, we summarize recent progress nanostructures, mechanics, their various implementations.

Language: Английский

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Sensing Picomolar Concentrations of RNA Using Switchable Plasmonic Chirality

Angewandte Chemie International Edition, Journal Year: 2018, Volume and Issue: 57(41), P. 13495 - 13498

Published: Aug. 7, 2018

Abstract Detecting small sequences of RNA in biological samples such as microRNA or viral demands highly sensitive and specific methods. Here, a reconfigurable DNA origami template has been used where chiral arrangement gold nanorods on the structure can lead to generation strong circular dichroism (CD). Switching cross‐like is achieved by addition nucleic acid sequences, which arrests one possible states molecular recognition. A sequence thus be detected through resulting changes plasmonic CD spectrum. We show selective detection target from hepatitis C virus genome. The binds complementary that part lock mechanism, leads formation defined state system with distinct optical response. With this approach, we were able detect at concentrations low 100 p m .

Language: Английский

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DNA Nanotechnology‐Based Biosensors and Therapeutics

Advanced Healthcare Materials, Journal Year: 2021, Volume and Issue: 10(15)

Published: June 3, 2021

Over the past few decades, DNA nanotechnology engenders a vast variety of programmable nanostructures utilizing Watson-Crick base pairing. Due to their precise engineering, unprecedented programmability, and intrinsic biocompatibility, cannot only interact with small molecules, nucleic acids, proteins, viruses, cancer cells, but also can serve as nanocarriers deliver different therapeutic agents. Such addressability innate enables use in various fields biomedical applications such biosensors therapy. This review is begun brief introduction development nanotechnology, followed by summary recent therapeutics. Finally, challenges opportunities for practical are discussed.

Language: Английский

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3D Patterning within Hydrogels for the Recreation of Functional Biological Environments

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(16)

Published: Feb. 11, 2021

Abstract Biological structures are inherently complex in nature. Structural hierarchy, chemical anisotropy, and compositional heterogeneity ubiquitous biological systems play a key role the functionality of living systems. For decades, methods such as soft lithography have enabled recreation arrangements through precise spatial control molecular patterns 2D. With technological advances increasing understanding structural biology, there has been an interest recreating organizations 3D. In this review, comprehensive summary latest technologies being used to create 3D functional molecules within hydrogels for tissue engineering applications is presented. The review divided into five groups defined according main driving force fabricate including light, design, microfluidics, printing, non‐contact forces (i.e. electric, magnetic, or acoustic fields self‐assembly).

Language: Английский

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Information Coding in a Reconfigurable DNA Origami Domino Array

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(31), P. 12991 - 12997

Published: April 18, 2020

DNA nanostructures with programmable nanoscale patterns has been achieved in the past decades, and molecular information coding (MIC) on those designed gained increasing attention for security. However, achieving steganography cryptography synchronously remains a challenge. Herein, we demonstrated MIC reconfigurable origami domino array (DODA), which can reconfigure intrinsic but keep DODA outline same steganography. When set of keys (DNA strands) are added, cryptographic data be translated into visible within DODA. More complex ASCII code 6×6 lattice is to demosntrate versatility Furthermore, an anti-counterfeiting approach based conformational transformation-mediated toehold strand displacement reaction protect from decoding falsification.

Language: Английский

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Bioinspired Supramolecular Catalysts from Designed Self-Assembly of DNA or Peptides

ACS Catalysis, Journal Year: 2020, Volume and Issue: 10(24), P. 14937 - 14958

Published: Dec. 4, 2020

Enzymes play vital roles in cellular functions by catalyzing biochemical reactions high activity and selectivity, which is largely attributed to the cooperation between exquisitely distributed functional groups at active sites. Reconstruction of enzymatic sites artificial systems, produce robust biocatalysts, has been a significant but challenging subject. Biomolecular self-assembly, typically with de novo designed peptide or DNA as building blocks, provides an effective avenue orient confine reactive into catalytically configuration mimic even rival catalytic performance native enzymes. In this Review, we discuss chemical structural properties self-assemblies, principles strategies designing that show hydrolase-, peroxidase-, oxidase-, aldolase-like activities. We focus on relationship control over structures performances. also highlight synergies components formation sites, well applications end, provide outlook obstacles, possible solutions, future directions, aspect modeling, improving performances, upgrading complexity

Language: Английский

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DNA Strand Displacement Reaction: A Powerful Tool for Discriminating Single Nucleotide Variants

Topics in Current Chemistry, Journal Year: 2020, Volume and Issue: 378(1)

Published: Jan. 2, 2020

Language: Английский

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