Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics

Gels, Journal Year: 2024, Volume and Issue: 10(3), P. 162 - 162

Published: Feb. 21, 2024

Extracellular vesicles (EVs), especially exosomes, have shown great therapeutic potential in the treatment of diseases, as they can target cells or tissues. However, effect EVs is limited due to susceptibility immune system clearance during transport vivo. Hydrogels become an ideal delivery platform for their good biocompatibility and porous structure. This article reviews preparation application EVs-loaded hydrogels a cell-free therapy strategy diseases. The also discusses challenges future outlook hydrogels.

Language: Английский

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Progress of Gene‐Functionalized Regenerative Material Repair Intervertebral Disc Degeneration

Small Science, Journal Year: 2024, Volume and Issue: 4(6)

Published: March 18, 2024

Intervertebral disc degeneration (IDD) is widely recognized as the primary culprit of chronic low back pain. Restoring deteriorated intervertebral (IVD) and alleviating IDD‐induced pain are remaining enormous challenges. There a genetic susceptibility to IDD, gene therapy has some therapeutic potential. However, traditional still certain drawbacks, including host immunity, temporary release, suppression medication function. Although regenerative materials can effectively improve local microenvironment, they cannot treat IDD from root. Gene‐functionalized material (GRM) constructed based on physical embedding or forming chemical bonds by introducing particular genes, such pDNA, siRNA, mRNA, miRNA, into regenerated materials. The findings demonstrate that GRM not only enhances safety controllability therapy, but also repairs overcoming constraint simple reverse disease's progression This article provided brief overview physiological pathological features IVD, available treatment options, their limitations. Then, significance GRM‐based proposed, future challenges development in this field finally prospected.

Language: Английский

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Effective delivery of miR-150-5p with nucleus pulposus cell-specific nanoparticles attenuates intervertebral disc degeneration

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 27, 2024

Abstract Background The use of gene therapy to deliver microRNAs (miRNAs) has gradually translated preclinical application for the treatment intervertebral disc degeneration (IDD). However, effects miRNAs are hindered by short half-life time and poor cellular uptake, owing lack efficient delivery systems. Here, we investigated nucleus pulposus cell (NPC) specific aptamer-decorated polymeric nanoparticles that can load miR-150-5p IDD treatment. Methods role during development was examined knockout (KO) mice. Histological analysis undertaken in specimens. functional mechanism qRT-PCR assay, Western blot, coimmunoprecipitation immunofluorescence. NPC designed, its penetration, stability safety were evaluated. progression assessed radiological including X-ray MRI, after annulus fibrosus needle puncture surgery with manipulation intradiscal injection nanoparticles. investigations into interaction between aptamer receptor conducted using mass spectrometry, molecular docking dynamics simulations. Results We NPC-specific bind Furthermore, detected nanoparticle-loaded inhibitors alleviated senescence vitro, sustained more than 3 months vivo. microenvironment NPCs improves endo/lysosomal escape miRNAs, greatly inhibiting secretion senescence-associated factors subsequent NPCs. Importantly, delivering attenuated puncture-induced mouse models targeting FBXW11 TAK1 ubiquitination, resulting downregulation NF-kB signaling pathway activity. Conclusions NPC-targeting show favorable therapeutic efficacy may constitute a promising IDD. Graphical

Language: Английский

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Sinomenine Ameliorates IL-1β-Induced Intervertebral Disc Degeneration in Rats Through Suppressing Inflammation and Oxidative Stress via Keap1/Nrf2/NF-κB Signaling Pathways

Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 4777 - 4791

Published: Oct. 1, 2023

Purpose: To investigate the molecular mechanism underlying inhibitory effect of sinomenine (SN) on interleukin-1β (IL-1β)-induced apoptosis in nucleus pulposus cells (NPCs), and to evaluate potential role SN preventing intervertebral disk degeneration (IDD). Methods: The Rat NPCs were cultured vitro identified using Hematoxylin-Eosin (HE) staining, toluidine blue immunofluorescence analysis. pretreated with or without SN, then induced IL-1β assess cell viability, ROS levels, apoptotic rates, wound healing ability. Relevant protein expression was detected Elisa, qPCR Western Blot techniques. either alone combination Nrf2-IN-1 SC, before being undergo by IL-1β. Apoptosis Hoechst while techniques assessed expression. caudal discs IDD, injection, co-injected levels IDD evaluated HE staining modified saffron-O-fix green cartilage staining. qPCR, Results: significantly reduced NPC activity, accumulation apoptosis, decreased rate, promoted secretion inflammatory factors, inhibited extracellular matrix synthesis. However, pretreatment effectively reversed these effects. Inhibition Keap1/Nrf2 signaling pathway activation NF-κB attenuated cytoprotective effects increased apoptosis. Acupuncture combined injection markedly disc rat spine, upregulated factors secretion, downregulated intervention notably enhanced antioxidant enzyme outcomes. Conclusion: can prevent IL-1β-induced ameliorate activating inhibiting pathway. Keywords: sinomenine, degeneration, cells, Nrf2,

Language: Английский

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Analysis of global research hotspots and trends in immune cells in intervertebral disc degeneration: A bibliometric study

Human Vaccines & Immunotherapeutics, Journal Year: 2023, Volume and Issue: 19(3)

Published: Nov. 9, 2023

Intervertebral disc degeneration is an important pathological basis for spinal degenerative diseases. The imbalance of the immune microenvironment and involvement cells has been shown to lead nucleus pulposus death. This article presents a bibliometric analysis studies on in IDD order clarify current status hotspots. We searched WOSCC, Scopus PubMed databases from 01/01/2001 08/03/2023. analyzed visualized content using software such as Citespace, Vosviewer bibliometrix. study found that number annual publications increasing year year. journal Spine had highest articles citations. country/regions showed China publications, USA citations total link strength. institutional Shanghai Jiao Tong University Huazhong Science Technology Tokai citations, Bern Sakai D Risbud MV publications. strength, results keyword suggested research hotspots future directions continue be mechanisms IDD, therapeutic role tissue engineering IDD.

Language: Английский

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