Langmuir,
Journal Year:
2024,
Volume and Issue:
40(29), P. 15205 - 15213
Published: July 11, 2024
The
size
or
the
curvature
of
nanoparticles
(NPs)
plays
an
important
role
in
regulating
composition
protein
corona.
However,
molecular
mechanisms
how
affects
interaction
NPs
with
serum
proteins
still
remain
elusive.
In
this
study,
we
employ
all-atom
dynamics
simulations
to
investigate
interactions
between
two
typical
and
PEGylated
Au
three
different
surface
curvatures
(0,
0.1,
0.5
nm
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 7, 2024
The
protein
corona
formed
on
nanoparticles
(NPs)
has
potential
as
a
valuable
diagnostic
tool
for
improving
plasma
proteome
coverage.
Here,
we
show
that
spiking
small
molecules,
including
metabolites,
lipids,
vitamins,
and
nutrients
into
can
induce
diverse
patterns
otherwise
identical
NPs,
significantly
enhancing
the
depth
of
profiling.
coronas
polystyrene
NPs
when
exposed
to
treated
with
an
array
molecules
allows
detection
1793
proteins
marking
8.25-fold
increase
in
number
quantified
compared
alone
(218
proteins)
2.63-fold
relative
untreated
(681
proteins).
Furthermore,
discovered
adding
1000
µg/ml
phosphatidylcholine
could
singularly
enable
897
proteins.
At
this
specific
concentration,
selectively
depletes
four
most
abundant
proteins,
albumin,
thus
reducing
dynamic
range
enabling
lower
abundance.
Employing
optimized
data-independent
acquisition
approach,
inclusion
leads
1436
single
sample.
Our
molecular
dynamics
results
reveal
interacts
albumin
via
hydrophobic
interactions,
H-bonds,
water
bridges.
addition
also
enables
337
additional
proteoforms
using
top-down
proteomics
approach.
Given
critical
role
biomarker
discovery
disease
monitoring,
anticipate
widespread
adoption
methodology
identification
clinical
translation
biomarkers.
International Journal of Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
654, P. 123987 - 123987
Published: March 11, 2024
It
is
well
known
that
protein
corona
affects
the
"biological
identity"
of
nanoparticles
(NPs),
which
has
been
seen
as
both
a
challenge
and
an
opportunity.
Approaches
have
moved
from
avoiding
adsorption
to
trying
direct
it,
taking
advantage
formation
favorably
modify
pharmacokinetic
parameters
NPs.
Although
promising,
results
obtained
with
engineered
NPs
still
need
be
completely
understood.
While
much
effort
put
into
understanding
how
surface
nanomaterials
absorption,
less
about
proteins
can
affect
due
their
specific
physicochemical
properties.
This
review
addresses
this
knowledge
gap,
examining
key
factors
influencing
formation,
highlighting
current
challenges
in
studying
protein–protein
interactions,
discussing
future
perspectives
field.
Chem & Bio Engineering,
Journal Year:
2024,
Volume and Issue:
1(9), P. 757 - 772
Published: Oct. 3, 2024
Nanoparticles
entering
biological
systems
or
fluids
inevitably
adsorb
biomolecules,
such
as
protein,
on
their
surfaces,
forming
a
protein
corona.
Ensuing,
the
corona
endows
nanoparticles
with
new
identity
and
impacts
interaction
between
systems.
Hence,
development
of
reliable
techniques
for
isolation
analysis
is
key
understanding
behaviors
nanoparticles.
First,
this
review
systematically
outlines
approach
isolating
corona,
including
centrifugation,
magnetic
separation,
size
exclusion
chromatography,
flow-field-flow
fractionation,
other
emerging
methods.
Next,
we
qualitative
quantitative
characterization
methods
Finally,
underscore
necessary
steps
to
advance
efficiency
fidelity
nanoparticle
surfaces.
We
anticipate
that
these
insights
into
methodologies
will
profoundly
influence
technologies
aimed
at
elucidating
bionano
interactions
role
in
various
biomedical
applications.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
The
protein
corona
effect
refers
to
the
phenomenon
wherein
nanomaterials
in
bloodstream
are
coated
by
serum
proteins,
yet
how
coronated
interact
with
blood
vessels
and
its
toxicity
implications
remain
poorly
understood.
In
this
study,
we
investigated
corona-related
vessel
using
an
all-humanized
assay
integrating
organoids
patient-derived
serum.
Initially,
screened
various
discern
parameters
including
size,
morphology,
hydrophobicity,
surface
charge,
chirality-dependent
difference
influence
their
uptake
organoids.
For
showing
substantial
differences
uptake,
was
analyzed
label-free
mass
spectra.
Our
findings
revealed
involvement
of
cancer
staging-related
cytoskeleton
components
mediating
preferential
cells,
endothelial
mural
cells.
Additionally,
a
transcriptome
study
conducted
elucidate
nanomaterials.
We
confirmed
that
provoke
remodeling
at
both
transcriptional
translational
levels,
impacting
pathways
such
as
PI3K-Akt/Hippo/Wnt,
membraneless
organelle
integrity,
respectively.
further
demonstrated
potential
can
be
harnessed
synergize
antiangiogenesis
therapeutics
improve
outcomes.
anticipate
will
provide
guidance
for
safe
use
nanomedicine
future.
Journal of Biomaterials Science Polymer Edition,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 27
Published: Jan. 9, 2025
Molecular
Dynamics
(MD)
simulations
are
now
widely
utilized
in
pharmaceutical
nanotechnology
to
gain
deeper
understanding
of
nanoscale
processes
imperative
drug
design.
This
review
has
also
detailed
how
MD
simulation
can
be
employed
the
study
drug-nanocarrier
interactions,
controlling
release
chemical
compounds
from
delivery
systems
and
increasing
solubility
bioavailability
nanocarriers.
Furthermore,
contributes
examining
systems,
measuring
toxic
effects,
determining
biocompatibility
nanomedical
systems.
With
incorporation
artificial
intelligence
use
hybrid
gone
a
step
ahead
model
other
niches
biology
that
make
tremendous
opening
develop
highly
selective
nanomedications.
Nevertheless,
with
well-known
issues
such
as
computational
constraints
discrepancy
between
silico
experiment
results,
remains
work
progress,
considerable
promise
for
replacing
or
supplementing
existing
approaches
development
precision
medicine
nanomedicine,
continued
progression
healthcare
hopeful.
Molecular Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Neuronanomedicine
harnesses
nanoparticle
technology
for
the
treatment
of
neurological
disorders.
An
unavoidable
consequence
delivery
to
biological
systems
is
formation
a
protein
corona
on
surface.
Despite
well-established
influence
behavior
and
fate,
as
well
FDA
approval
neuro-targeted
nanotherapeutics,
effect
physiologically
relevant
nanoparticle-brain
cell
interactions
insufficiently
explored.
Indeed,
less
than
1%
studies
have
investigated
coronas
formed
in
cerebrospinal
fluid
(CSF),
surrounding
brain.
Herein,
we
utilize
two
clinically
polymeric
nanoparticles
(PLGA
PLGA-PEG)
evaluate
serum
CSF
coronas.
LC-MS
analysis
revealed
distinct
compositions,
with
selective
enrichment/depletion
profiles.
Enhanced
association
precoated
particles
brain
cells
demonstrates
importance
selecting
fluids
more
accurately
study
subsequent
nanoparticle-cell
interactions,
paving
way
improved
engineering
vivo
applications.
