Effect of Nanoparticle Curvature on Its Interaction with Serum Proteins DOI
Yue‐wen Yin, Yu‐qiang Ma, Hong‐ming Ding

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(29), P. 15205 - 15213

Published: July 11, 2024

The size or the curvature of nanoparticles (NPs) plays an important role in regulating composition protein corona. However, molecular mechanisms how affects interaction NPs with serum proteins still remain elusive. In this study, we employ all-atom dynamics simulations to investigate interactions between two typical and PEGylated Au three different surface curvatures (0, 0.1, 0.5 nm

Language: Английский

Small molecule modulation of protein corona for deep plasma proteome profiling DOI Creative Commons
Ali Akbar Ashkarran, Hassan Gharibi,

Seyed Amirhossein Sadeghi

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 7, 2024

The protein corona formed on nanoparticles (NPs) has potential as a valuable diagnostic tool for improving plasma proteome coverage. Here, we show that spiking small molecules, including metabolites, lipids, vitamins, and nutrients into can induce diverse patterns otherwise identical NPs, significantly enhancing the depth of profiling. coronas polystyrene NPs when exposed to treated with an array molecules allows detection 1793 proteins marking 8.25-fold increase in number quantified compared alone (218 proteins) 2.63-fold relative untreated (681 proteins). Furthermore, discovered adding 1000 µg/ml phosphatidylcholine could singularly enable 897 proteins. At this specific concentration, selectively depletes four most abundant proteins, albumin, thus reducing dynamic range enabling lower abundance. Employing optimized data-independent acquisition approach, inclusion leads 1436 single sample. Our molecular dynamics results reveal interacts albumin via hydrophobic interactions, H-bonds, water bridges. addition also enables 337 additional proteoforms using top-down proteomics approach. Given critical role biomarker discovery disease monitoring, anticipate widespread adoption methodology identification clinical translation biomarkers.

Language: Английский

Citations

9

Unveiling the challenges of engineered protein corona from the proteins’ perspective DOI Creative Commons

Cintia Marques,

Gerrit Borchard, Olivier Jordan

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 654, P. 123987 - 123987

Published: March 11, 2024

It is well known that protein corona affects the "biological identity" of nanoparticles (NPs), which has been seen as both a challenge and an opportunity. Approaches have moved from avoiding adsorption to trying direct it, taking advantage formation favorably modify pharmacokinetic parameters NPs. Although promising, results obtained with engineered NPs still need be completely understood. While much effort put into understanding how surface nanomaterials absorption, less about proteins can affect due their specific physicochemical properties. This review addresses this knowledge gap, examining key factors influencing formation, highlighting current challenges in studying protein–protein interactions, discussing future perspectives field.

Language: Английский

Citations

8

The nanoparticle-Protein Corona untold history (1907–2007) DOI
Eudald Casals, Michele Vitali, Víctor Puntes

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 58, P. 102435 - 102435

Published: Aug. 5, 2024

Language: Английский

Citations

7

Protein Corona of Nanoparticles: Isolation and Analysis DOI Creative Commons

Yinuo Sun,

Yaxin Zhou, Mubashar Rehman

et al.

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 1(9), P. 757 - 772

Published: Oct. 3, 2024

Nanoparticles entering biological systems or fluids inevitably adsorb biomolecules, such as protein, on their surfaces, forming a protein corona. Ensuing, the corona endows nanoparticles with new identity and impacts interaction between systems. Hence, development of reliable techniques for isolation analysis is key understanding behaviors nanoparticles. First, this review systematically outlines approach isolating corona, including centrifugation, magnetic separation, size exclusion chromatography, flow-field-flow fractionation, other emerging methods. Next, we qualitative quantitative characterization methods Finally, underscore necessary steps to advance efficiency fidelity nanoparticle surfaces. We anticipate that these insights into methodologies will profoundly influence technologies aimed at elucidating bionano interactions role in various biomedical applications.

Language: Английский

Citations

7

Protein Coronation-Induced Cancer Staging-Dependent Multilevel Cytotoxicity: An All-Humanized Study in Blood Vessel Organoids DOI
Chan Wang,

Yingyi Quan,

Jiang Jiang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The protein corona effect refers to the phenomenon wherein nanomaterials in bloodstream are coated by serum proteins, yet how coronated interact with blood vessels and its toxicity implications remain poorly understood. In this study, we investigated corona-related vessel using an all-humanized assay integrating organoids patient-derived serum. Initially, screened various discern parameters including size, morphology, hydrophobicity, surface charge, chirality-dependent difference influence their uptake organoids. For showing substantial differences uptake, was analyzed label-free mass spectra. Our findings revealed involvement of cancer staging-related cytoskeleton components mediating preferential cells, endothelial mural cells. Additionally, a transcriptome study conducted elucidate nanomaterials. We confirmed that provoke remodeling at both transcriptional translational levels, impacting pathways such as PI3K-Akt/Hippo/Wnt, membraneless organelle integrity, respectively. further demonstrated potential can be harnessed synergize antiangiogenesis therapeutics improve outcomes. anticipate will provide guidance for safe use nanomedicine future.

Language: Английский

Citations

1

Molecular dynamics in pharmaceutical nanotechnology: simulating interactions and advancing applications DOI
Anand B. Mundada, Prashanta Chandra Pradhan, Rajapandi Raju

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 27

Published: Jan. 9, 2025

Molecular Dynamics (MD) simulations are now widely utilized in pharmaceutical nanotechnology to gain deeper understanding of nanoscale processes imperative drug design. This review has also detailed how MD simulation can be employed the study drug-nanocarrier interactions, controlling release chemical compounds from delivery systems and increasing solubility bioavailability nanocarriers. Furthermore, contributes examining systems, measuring toxic effects, determining biocompatibility nanomedical systems. With incorporation artificial intelligence use hybrid gone a step ahead model other niches biology that make tremendous opening develop highly selective nanomedications. Nevertheless, with well-known issues such as computational constraints discrepancy between silico experiment results, remains work progress, considerable promise for replacing or supplementing existing approaches development precision medicine nanomedicine, continued progression healthcare hopeful.

Language: Английский

Citations

1

Nanoparticle Association with Brain Cells Is Augmented by Protein Coronas Formed in Cerebrospinal Fluid DOI
Claire Rennie, Nabila Morshed, Matthew Faria

et al.

Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Neuronanomedicine harnesses nanoparticle technology for the treatment of neurological disorders. An unavoidable consequence delivery to biological systems is formation a protein corona on surface. Despite well-established influence behavior and fate, as well FDA approval neuro-targeted nanotherapeutics, effect physiologically relevant nanoparticle-brain cell interactions insufficiently explored. Indeed, less than 1% studies have investigated coronas formed in cerebrospinal fluid (CSF), surrounding brain. Herein, we utilize two clinically polymeric nanoparticles (PLGA PLGA-PEG) evaluate serum CSF coronas. LC-MS analysis revealed distinct compositions, with selective enrichment/depletion profiles. Enhanced association precoated particles brain cells demonstrates importance selecting fluids more accurately study subsequent nanoparticle-cell interactions, paving way improved engineering vivo applications.

Language: Английский

Citations

1

Splenic response to protein corona of nanoparticles in vivo DOI
Can Chen, Yueping Li, Dandan Zhou

et al.

Nano Today, Journal Year: 2025, Volume and Issue: 62, P. 102676 - 102676

Published: March 1, 2025

Language: Английский

Citations

1

Ai-driven prediction of cardio-oncology biomarkers through protein corona analysis DOI
Avirup Guha,

Seyed Amirhossein Sadeghi,

Harikrishnan Hyma Kunhiraman

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 509, P. 161134 - 161134

Published: March 1, 2025

Language: Английский

Citations

1

Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery DOI Creative Commons
Yuichi Suzuki,

Mai Yakuwa,

Mina Sato

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113687 - 113687

Published: April 1, 2025

Language: Английский

Citations

1