Heterogeneous DNA hydrogel loaded with Apt02 modified tetrahedral framework nucleic acid accelerated critical-size bone defect repair

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 1 - 16

Published: Jan. 18, 2024

Segmental bone defects, stemming from trauma, infection, and tumors, pose formidable clinical challenges. Traditional repair materials, such as autologous allogeneic grafts, grapple with limitations including source scarcity immune rejection risks. The advent of nucleic acid nanotechnology, particularly the use DNA hydrogels in tissue engineering, presents a promising solution, attributed to their biocompatibility, biodegradability, programmability. However, these hydrogels, typically hindered by high gelation temperatures (∼46 °C) construction costs, limit cell encapsulation broader application. Our research introduces novel polymer-modified hydrogel, developed using which gels at more biocompatible temperature 37 °C is cost-effective. This hydrogel then incorporates tetrahedral Framework Nucleic Acid (tFNA) enhance osteogenic mineralization. Furthermore, considering modifiability tFNA, we modified its chains Aptamer02 (Apt02), an aptamer known foster angiogenesis. dual approach significantly accelerates differentiation marrow stromal cells (BMSCs) angiogenesis human umbilical vein endothelial (HUVECs), sequencing confirming targeting efficacy, respectively. In vivo experiments rats critical-size cranial defects demonstrate effectiveness enhancing new formation. innovation not only offers viable solution for repairing segmental but also opens avenues future advancements organoids construction, marking significant advancement engineering regenerative medicine.

Language: Английский

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Research Progress in Hydrogels for Cartilage Organoids

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

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DNA-Based Hydrogels for Bone Regeneration: A Promising Tool for Bone Organoids

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101502 - 101502

Published: Jan. 19, 2025

DNA-based hydrogels stand out for bone regeneration due to their exceptional biocompatibility and programmability. These facilitate the formation of spatial structures through bulk hydrogel fabricating, microsphere formatting, 3D printing. Furthermore, microenvironment can be finely tuned by leveraging degradation products, nanostructure, targeting, delivery capabilities inherent materials. In this review, we underscore advantages hydrogels, detailing composition, gelation techniques, structure optimization. We then delineate three critical elements in promotion using hydrogels: (i) osteogenesis driven phosphate ions, plasmids, oligodeoxynucleotides (ODNs) that enhance mineralization promote gene protein expression; (ii) vascularization facilitated tetrahedral DNA nanostructures (TDNs) aptamers, which boosts expression targeted release; (iii) immunomodulation achieved loaded factors, TDNs, bound ions stimulate macrophage polarization exhibit antibacterial properties. With these properties, used construct organoids, providing an innovative tool disease modeling therapeutic applications tissue engineering. Finally, discuss current challenges future prospects, emphasizing potential impacts regenerative medicine.

Language: Английский

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DNA-encoded Dynamic Hydrogels for 3D Bioprinted Cartilage Organoids

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101509 - 101509

Published: Jan. 22, 2025

Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiological functions is crucial regenerative medicine, drug screening, disease modeling. However, commonly used matrix bioinks lack reversible cross-linking precise controllability, hindering cellular regulation. Thus, encoding adaptive cultivating an attractive idea. DNA, with its ability be intricately encoded reversibly cross-linked into hydrogels, offers manipulation at both molecular spatial structural levels. This endows hydrogels viscoelasticity, printability, cell recognition, stimuli responsiveness. paper elaborates on strategies encode bioink via emphasizing regulation predictable properties resulting interactions behavior. The significance these construction highlighted. Finally, we discuss challenges future prospects using DNA-encoded 3D bioprinted organoids, underscoring their potential impact advancing biomedical applications.

Language: Английский

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Decoding bone-inspired and cell-instructive cues of scaffolds for bone tissue engineering

Engineered Regeneration, Journal Year: 2023, Volume and Issue: 5(1), P. 21 - 44

Published: Oct. 27, 2023

Bone fractures are common occurrence in clinical settings, creating a high demand for effective repair material. Unfortunately, limited graft availability, donor site morbidities, unpredictable outcomes, immunologic reactions, infection risks, and geometrical mismatching concerns hampered tissue use underscored the need scaffolds more bone reconstructions due to their tunable properties. Significant progress has been carried out past decade fields of nanoceramics synthesis, bioconjugate chemistry, composite material processing. This review outlines hierarchical structures biology tissue, materialistic components (bioceramics, polymers, bioactive drugs), featured scaffolding strategies (nanofibers, hydrogels, aerogels, bioprinting, fiber-reinforced composite), emphasis that physiochemical characteristics should be used as an inspiration scaffold design. discussed how differences materiobiological aspects scaffolds, such polymer/bioceramic nanocomposite, mineralized matrix-rich 3D microenvironmental cues, pore space mechanical usage physical stimulation (magnetic, electroactive, photoactivated cues), surface cues (wettability, roughness, textured, charge), biointerface (cell–biomaterial interactions, cell-selective homing, cell regulatory strategies) modulate cellular biological response engineering. study further challenges benefits integrating

Language: Английский

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3D printed Nanohydroxyapatite/Polyamide 66 scaffolds with balanced mechanical property and osteogenic ability for bone repair

Materials & Design, Journal Year: 2024, Volume and Issue: 241, P. 112896 - 112896

Published: April 1, 2024

The biomaterial requirements for bone tissue repair are extremely strict. It not only requires the to have good biocompatibility and biological activity, but also sufficient mechanical strength. Polyamide 66 (PA66) nano-hydroxyapatite (n-HA) been widely investigated in engineering scaffold, however, its composites require further research. In this study, 3D printing technology was utilized fabricate personalized n-HA/PA66 scaffold. properties of composite can be adjusted by configuring different raw material components applying porosity. found that 50 % porosity scaffold showed a uniform porous structure compressive strength 33.9 MPa. exhibited excellent while improving properties, vitro cell tests indicated printed promoted differentiation marrow mesenchymal stem cells (BMSCs) into osteoblasts. vivo animal model proved endogenous regeneration rabbit patellar defects, with newly formed volume 20.9 after two months implantation. This research proposed polyamide/nano-hydroxyapatite balanced property osteogenic ability customized repair, it has promising application prospects.

Language: Английский

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Advances in Biomedical Applications of Solution Blow Spinning

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14757 - 14757

Published: Sept. 29, 2023

In recent years, Solution Blow Spinning (SBS) has emerged as a new technology for the production of polymeric, nanocomposite, and ceramic materials in form nano microfibers, with similar features to those achieved by other procedures. The advantages SBS over spinning methods are fast generation fibers simplicity experimental setup that opens up possibility their on-site production. While producing large number nanofibers short time is crucial factor large-scale manufacturing, situ generation, example, sprayable, multifunctional dressings, capable releasing embedded active agents on wounded tissue, or use operating rooms prevent hemostasis during surgical interventions, open wide range possibilities. interest this evident from growing patents issued articles published last few years. Our focus review biomedicine-oriented applications based collection most relevant scientific papers date. Drug delivery, 3D culturing, regenerative medicine, fabrication biosensors some areas which been explored, frequently at proof-of-concept level. promising results obtained demonstrate potential biomedical pharmaceutical fields.

Language: Английский

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Immunomodulatory nanomedicine for osteoporosis: Current practices and emerging prospects

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 179, P. 13 - 35

Published: March 16, 2024

Language: Английский

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Mussel-inspired antimicrobial hydrogel with cellulose nanocrystals/tannic acid modified silver nanoparticles for enhanced calvarial bone regeneration

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 132419 - 132419

Published: May 15, 2024

Language: Английский

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Enhanced bone regeneration with bioprinted GelMA/Bentonite scaffolds inspired by bone matrix

Virtual and Physical Prototyping, Journal Year: 2024, Volume and Issue: 19(1)

Published: April 27, 2024

In bone tissue engineering, the search for improved repair methods is crucial, given drawbacks of traditional strategies like donor site issues and immune rejection. Addressing these challenges, this paper introduces an innovative GelMA/Bentonite composite bioink 3D bioprinting, designed to create scaffolds that closely emulate native tissue. GelMA selected its biocompatibility modifiable mechanics, while Bentonite's mineral richness ion exchange capacity are harnessed enhance scaffold structure promote osteogenic microenvironment. This research marks inaugural incorporation Bentonite into a bioink, significant stride forward established safety in pharmaceuticals versatility across industries. formulation signifies breakthrough aiming improve osteointegration regeneration Combining with key step creating bioinks healing, potentially transforming scaffold-based pioneering use natural nanomaterials medicine.

Language: Английский

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