Journal of Biochemical and Molecular Toxicology,
Journal Year:
2024,
Volume and Issue:
38(6)
Published: May 21, 2024
Abstract
The
aim
of
this
investigation
was
to
evaluate
the
differential
expression
sterol
O‐acyltransferase
1
(SOAT1)
protein
in
gallbladder
cancer
tissues
and
cells,
investigate
impact
Avastin
on
proliferation,
migration,
invasion
capabilities
its
potential
induce
cell
apoptosis.
Immunohistochemical
analysis
samples
from
145
patients
conducted,
along
with
SOAT1
protein,
mRNA
levels,
cholesterol
content
lines
SGC‐996,
NOZ,
(GBC)‐SD
using
Western
blot
q‐PCR
techniques.
Furthermore,
effects
these
were
studied,
ability
apoptosis
evaluated
flow
cytometry,
blot,
immunohistochemical
methods.
Additionally,
gene
pathway
performed,
synergistic
therapeutic
combined
gemcitabine
tested
a
xenograft
model.
study
found
that
significantly
upregulated
GBC
positively
correlated
lymph
node
metastasis
TNM
staging.
In
vitro
experiments
demonstrated
inhibited
SGC‐996
GBC‐SD
induced
RNA
sequencing
revealed
multiple
differentially
expressed
genes
cells
treated
Avastin,
primarily
enriched
biological
pathways
such
as
signaling
transduction,
malignant
tumors,
immune
system.
vivo,
confirmed
could
effectively
suppress
tumor
growth
model
enhanced
treatment
efficacy
when
used
combination
gemcitabine.
Overall,
findings
provide
new
insights
strategies
for
targeted
therapy
cancer.
Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
31, P. 101502 - 101502
Published: Jan. 19, 2025
DNA-based
hydrogels
stand
out
for
bone
regeneration
due
to
their
exceptional
biocompatibility
and
programmability.
These
facilitate
the
formation
of
spatial
structures
through
bulk
hydrogel
fabricating,
microsphere
formatting,
3D
printing.
Furthermore,
microenvironment
can
be
finely
tuned
by
leveraging
degradation
products,
nanostructure,
targeting,
delivery
capabilities
inherent
materials.
In
this
review,
we
underscore
advantages
hydrogels,
detailing
composition,
gelation
techniques,
structure
optimization.
We
then
delineate
three
critical
elements
in
promotion
using
hydrogels:
(i)
osteogenesis
driven
phosphate
ions,
plasmids,
oligodeoxynucleotides
(ODNs)
that
enhance
mineralization
promote
gene
protein
expression;
(ii)
vascularization
facilitated
tetrahedral
DNA
nanostructures
(TDNs)
aptamers,
which
boosts
expression
targeted
release;
(iii)
immunomodulation
achieved
loaded
factors,
TDNs,
bound
ions
stimulate
macrophage
polarization
exhibit
antibacterial
properties.
With
these
properties,
used
construct
organoids,
providing
an
innovative
tool
disease
modeling
therapeutic
applications
tissue
engineering.
Finally,
discuss
current
challenges
future
prospects,
emphasizing
potential
impacts
regenerative
medicine.
Bone Research,
Journal Year:
2024,
Volume and Issue:
12(1)
Published: Nov. 20, 2024
Abstract
The
concept
and
development
of
bone/cartilage
organoids
are
rapidly
gaining
momentum,
providing
opportunities
for
both
fundamental
translational
research
in
bone
biology.
Bone/cartilage
organoids,
essentially
miniature
tissues
grown
vitro,
enable
the
study
complex
cellular
interactions,
biological
processes,
disease
pathology
a
representative
controlled
environment.
This
review
provides
comprehensive
up-to-date
overview
field,
focusing
on
strategies
organoid
construction
strategies,
progresses
research,
potential
applications.
We
delve
into
significance
selecting
appropriate
cells,
matrix
gels,
cytokines/inducers,
techniques.
Moreover,
we
explore
role
advancing
our
understanding
reconstruction,
modeling,
drug
screening,
prevention,
treatment
strategies.
While
acknowledging
these
discuss
inherent
challenges
limitations
field
propose
solutions,
including
use
bioprinting
induction,
AI
improved
screening
exploration
assembloids
more
complex,
multicellular
models.
believe
that
with
continuous
refinement
standardization,
can
profoundly
impact
patient-specific
therapeutic
interventions
lead
way
regenerative
medicine.
Bioactive Materials,
Journal Year:
2024,
Volume and Issue:
38, P. 438 - 454
Published: May 12, 2024
Spinal
cord
injury
(SCI)
is
a
traumatic
condition
that
results
in
impaired
motor
and
sensory
function.
Ferroptosis
one
of
the
main
causes
neural
cell
death
loss
neurological
function
spinal
cord,
ferroptosis
inhibitors
are
effective
reducing
inflammation
repairing
SCI.
Although
human
umbilical
mesenchymal
stem
cells
(Huc-MSCs)
can
ameliorate
inflammatory
microenvironments
promote
regeneration
SCI,
their
efficacy
greatly
limited
by
local
microenvironment
after
Therefore,
this
study,
we
constructed
drug-release
nanoparticle
system
with
synergistic
Huc-MSCs
inhibitor,
which
anchored
Tz-A6
peptide
based
on
CD44-targeting
sequence,
combined
reactive
oxygen
species
(ROS)-responsive
drug
nanocarrier
mPEG-b-Lys-BECI-TCO
at
other
end
for
SCI
repair.
Meanwhile,
also
modified
classic
inhibitor
Ferrostatin-1
(Fer-1)
synthesized
new
prodrug
Feborastatin-1
(Feb-1).
The
showed
treatment
regimen
significantly
inhibited
response
promoted
recovery
rats
This
study
developed
combination
therapy
provides
strategy
construction
drug-coordinated
system.
Nano Letters,
Journal Year:
2024,
Volume and Issue:
24(17), P. 5154 - 5164
Published: April 11, 2024
Developing
novel
strategies
for
defeating
osteoporosis
has
become
a
world-wide
challenge
with
the
aging
of
population.
In
this
work,
supramolecular
nanoagonists
(NAs),
constructed
from
alkaloids
and
phenolic
acids,
emerge
as
carrier-free
nanotherapy
efficacious
treatment.
These
precision
are
formed
through
self-assembly
berberine
(BER)
chlorogenic
acid
(CGA),
utilizing
noncovalent
electrostatic,
π–π,
hydrophobic
interactions.
This
assembly
results
in
100%
drug
loading
capacity
stable
nanostructure.
Furthermore,
resulting
weights
proportions
CGA
BER
within
NAs
meticulously
controlled
strong
consistency
when
CGA/BER
feed
ratio
is
altered
1:1
to
1:4.
As
anticipated,
our
themselves
could
passively
target
osteoporotic
bone
tissues
following
prolonged
blood
circulation,
modulate
Wnt
signaling,
regulate
osteogenic
differentiation,
ameliorate
loss
ovariectomy-induced
mice.
We
hope
work
will
open
new
strategy
design
efficient
herbal-derived
dealing
intractable
osteoporosis.
Giant,
Journal Year:
2024,
Volume and Issue:
19, P. 100298 - 100298
Published: June 3, 2024
The
field
of
bone
regeneration
has
witnessed
significant
advancements
with
the
exploration
and
incorporation
marine
biomaterials,
offering
promising
avenues
for
orthopaedic
dental
applications.
Marine
environments
are
a
rich
source
biological
materials
unique
properties
conducive
to
healing
regeneration.
Repurposing
reusing
some
waste
by-products
products
not
only
contribute
environmental
protection
but
also
drives
development
economy,
thereby
achieving
sustainable
development.
Moreover,
lower
production
costs
associated
abundant
availability
easy
processing
biomaterials
make
therapies
more
accessible
broader
population,
enhancing
global
health
equity.
By
exploring
current
research
progressions
on
recounting
their
sources,
properties,
mechanisms
action,
applications
in
research,
this
review
provides
comprehensive
overview
potential
challenges
future
Military Medical Research,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Dec. 5, 2024
Abstract
Bioactive
peptides
and
proteins
(BAPPs)
are
promising
therapeutic
agents
for
tissue
repair
with
considerable
advantages,
including
multifunctionality,
specificity,
biocompatibility,
biodegradability.
However,
the
high
complexity
of
microenvironments
their
inherent
deficiencies
such
as
short
half-live
susceptibility
to
enzymatic
degradation,
adversely
affect
efficacy
clinical
applications.
Investigating
fundamental
mechanisms
by
which
BAPPs
modulate
microenvironment
developing
rational
delivery
strategies
essential
optimizing
administration
in
distinct
repairs
facilitating
translation.
This
review
initially
focuses
on
through
influence
via
reactive
oxygen
species,
blood
lymphatic
vessels,
immune
cells,
cells.
Then,
a
variety
platforms,
scaffolds
hydrogels,
electrospun
fibers,
surface
coatings,
assisted
particles,
nanotubes,
two-dimensional
nanomaterials,
nanoparticles
engineered
summarized
incorporate
effective
repair,
modification
aimed
at
enhancing
loading
efficiencies
release
kinetics
also
reviewed.
Additionally,
can
be
precisely
regulated
endogenous
stimuli
(glucose,
enzymes,
pH)
or
exogenous
(ultrasound,
heat,
light,
magnetic
field,
electric
field)
achieve
on-demand
tailored
specific
needs.
Furthermore,
this
potential
across
various
types,
bone,
cartilage,
intervertebral
discs,
muscle,
tendons,
periodontal
tissues,
skin,
myocardium,
nervous
system
(encompassing
brain,
spinal
cord,
peripheral
nerve),
endometrium,
well
ear
ocular
tissue.
Finally,
current
challenges
prospects
discussed.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 30, 2024
Abstract
Mechanical
force
is
essential
for
bone
development,
homeostasis,
and
fracture
healing.
In
the
past
few
decades,
various
biomaterials
have
been
developed
to
provide
mechanical
signals
that
mimic
natural
microenvironment,
thereby
promoting
regeneration.
Bone
organoids,
emerging
as
a
novel
research
approach,
are
3D
micro‐bone
tissues
possess
ability
self‐renew
self‐organize,
exhibiting
biomimetic
spatial
characteristics.
Incorporating
mechano‐responsive
in
construction
of
organoids
presents
promising
avenue
simulating
microenvironment.
Therefore,
this
review
commences
by
elucidating
impact
on
health,
encompassing
both
cellular
interactions
alterations
structure.
Furthermore,
most
recent
applications
within
realm
tissue
engineering
highlighted.
Three
different
types
introduced
with
focus
their
responsive
mechanisms,
strategies,
efficacy
facilitating
Based
comprehensive
overview,
prospective
utilization
future
challenges
discussed.
As
organoid
technology
advances,
these
poised
become
powerful
tools
