Covalently Crosslinked Coacervate: Immobilization and Stabilization of Proteins with Enhanced Enzymatic Activity DOI Creative Commons
Mengmeng Zhao, Szu-Hao Cho,

X. Wu

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Thiol–ene photoclick chemistry produces chemically crosslinked coacervates with pH and salt tolerance that can encapsulate protect functional biologics.

Language: Английский

Peptide-Mediated Liquid-Liquid Phase Separation and Biomolecular Condensates DOI

Guangle Li,

Chengqian Yuan, Xuehai Yan

et al.

Soft Matter, Journal Year: 2025, Volume and Issue: 21(10), P. 1781 - 1812

Published: Jan. 1, 2025

Peptide-mediated liquid–liquid phase separation (LLPS) underpins the formation of dynamic biomolecular condensates, regulated by diverse molecular interactions, and highlights potential applications in drug delivery synthetic biology.

Language: Английский

Citations

4
Coacervate‐Derived Assembly of Poly(ethylene glycol) Nanoparticles for Combinational Tumor Therapy DOI Open Access
Hanru Liu, Rende Mu, Huimin Geng

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Coacervates have garnered significant attention as potential drug carriers. However, the instability resulting from their intrinsic membrane-free nature restricts application of coacervates in delivery. Herein, engineering poly(ethylene glycol) nanoparticles (PEG NPs) is reported using composed PEG and polyphenols templates, where subsequently cross-linked based on different chemistries (e.g., thiol-disulfide exchange, click chemistry, Schiff base reaction). The assembly strategy avoids template removal process resultant NPs exhibit excellent stability physiological environment compared to coacervates. presence enables loading various cargos including metal ions (i.e., Ru, Gd, Mn, Fe) molecules doxorubicin), which demonstrates promise magnetic resonance imaging combinational tumor therapy. This work provides a promising promote development coacervate-derived delivery system for biomedical applications.

Language: Английский

Citations

0
Intestinal nanoparticle delivery and cellular response: a review of the bidirectional nanoparticle-cell interplay in mucosa based on physiochemical properties DOI Creative Commons
Yu Wang,

Y Mo,

Yingwei Sun

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 1, 2024

Orally administered nanocarriers play an important role in improving druggability, promoting intestinal absorption, and enhancing therapeutic applications for the treatment of local systemic diseases. However, delivering efficiency cell response mucosa to orally is affected by physiological environment barriers gastrointestinal tract, physicochemical properties nanocarriers, their bidirectional interactions. Goblet cells secrete form extracellular mucus, which hinders movement nanoparticles. Meanwhile, epithelial may absorb NPs, allowing transcytosis or degradation. Conversely, nanoparticle-induced toxicity occur as a biological nanoparticle exposure. Additionally, immune functions secretions such mucin, peptide, cytokines also be altered. In this review, we discuss interactions between nanoparticles focusing on enterocytes goblet cells, M according essential crosstalk with cells. Furthermore, recent advances how physiochemical influence interplay, delivery, fate mucosa. Understanding different from perspective interaction provides support development, rational design, potency maximation, application advanced oral nanocarrier delivery systems.

Language: Английский

Citations

3
Magnoflorine alleviates dextran sulfate sodium‐induced ulcerative colitis via inhibiting JAK2/STAT3 signaling pathway DOI
Jianxia Wen, Yi Yang, Lu Li

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(9), P. 4592 - 4613

Published: July 30, 2024

Abstract Magnoflorine (Mag), a natural alkaloid component originating from the Ranunculaceae Juss . Family, has various of pharmacological activities. This study aimed to investigate therapeutic effects and potential mechanism Mag on dextran sulfate sodium (DSS)‐induced ulcerative colitis (UC) based comprehensive approaches. Therapeutic 3% DSS‐induced UC mice were analyzed. UHPLC‐Q‐TOF/MS was performed metabolites signaling pathway UC. Furthermore, predicted mRNA protein levels JAK2/STAT3 in colon tissue verified assessed by qRT‐PCR Western Blotting, respectively. presented down‐regulation serum biochemical indices, alleviating histological damage tissue. Serum untargeted metabolomics analysis showed that is mainly associated with regulation six biomarkers 11 pathways, which may be responsible for efficacy The “component–metabolites–targets” interactive network indicated exerts its anti‐UC effect regulating PTGS1 PTGS2, thereby arachidonic acid. Moreover, results could substantially decrease relative expression level Hub genes. In addition, it found inhibit pathway. present highlighted role ameliorated injury inhibiting These suggest an effective agent treatment

Language: Английский

Citations

2
A Novel Colon-Targeting Ratiometric Probe with Large Emission Shift for Imaging Peroxynitrite in Ulcerative Colitis DOI

Hui Zhang,

Sisi Chen,

Zhiqing Wang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(47), P. 18852 - 18858

Published: Nov. 15, 2024

Ulcerative colitis (UC) is an inflammatory disease that leads to the overexpression of peroxynitrite (ONOO

Language: Английский

Citations

2
Coacervation for biomedical applications: innovations involving nucleic acids DOI Creative Commons
Kimiasadat Mirlohi, Whitney C. Blocher McTigue

Soft Matter, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Coacervation emerges as a cutting-edge approach to enhance drug delivery, vaccines, and other therapeutics offer potential improvements in their efficacy. Figure created BioRender. Mirlohi, K. (2024) https://BioRender.com/c81q692.

Language: Английский

Citations

2
Comparative Study of pH-Responsive and Aggregation Stability of Bosutinib-Loaded Nanogels Comprising Gelatin Methacryloyl, Carboxymethyl Dextran, and Hyaluronic Acid for Controlled Drug Delivery in Colorectal Cancer: An Extensive In Vitro Investigation DOI
Sankha Bhattacharya, Shashikant B. Bagade, Preeti C. Sangave

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

This study investigates the use of pH-responsive nanogels for delivering Bosutinib (BOSU) in colon cancer treatment. Nanogels were formulated using three polymers: hyaluronic acid (HA), carboxymethyl dextran (CMD), and gelatin methacryloyl (GelMA). These achieved high drug entrapment efficiencies (80–90%) through polymer mixing with BOSU, followed by EDC/NHS cross-linking sonication. The stable, negative zeta potentials (−20 to −30 mV) particle sizes between 100 200 nm. Fourier-transform infrared analysis confirmed successful methacrylation GelMA nanogels. Sustained BOSU release at pH 5.0 was observed, resembling tumor environments, compared slower normal (7.4). Cytotoxicity tests showed 70–80% cell survival reduction HCT116 cells higher doses, GelMA-BOSU notably reduced migration. Antiangiogenic effects a chick chorioallantoic membrane model, highlighting potential these targeted delivery therapy.

Language: Английский

Citations

1
Covalently Crosslinked Coacervate: Immobilization and Stabilization of Proteins with Enhanced Enzymatic Activity DOI Creative Commons
Mengmeng Zhao, Szu-Hao Cho,

X. Wu

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Thiol–ene photoclick chemistry produces chemically crosslinked coacervates with pH and salt tolerance that can encapsulate protect functional biologics.

Language: Английский

Citations

0