Label-free microfluidic cell sorting and detection for rapid blood analysis

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(5), P. 1226 - 1257

Published: Jan. 1, 2023

Blood tests are considered as standard clinical procedures to screen for markers of diseases and health conditions. However, the complex cellular background (>99.9% RBCs) biomolecular composition often pose significant technical challenges accurate blood analysis. An emerging approach point-of-care diagnostics is utilizing "label-free" microfluidic technologies that rely on intrinsic cell properties fractionation disease detection without any antibody binding. A growing body evidence has also reported dysfunction their biophysical phenotypes complementary hematoanalyzer analysis (complete count) can provide a more comprehensive profiling. In this review, we will summarize recent advances in label-free separation different components including circulating tumor cells, leukocytes, platelets nanoscale extracellular vesicles. Label-free single morphology, spectrochemical properties, dielectric parameters characteristics novel blood-based biomarkers be presented. Next, highlight research efforts combine microfluidics with machine learning approaches enhance sensitivity specificity studies, well innovative solutions which capable fully integrated sorting Lastly, envisage current future outlook platforms high throughput multi-dimensional identify non-traditional diagnostics.

Language: Английский

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Capillarity Enabled Large‐Array Liquid Metal Electrodes for Compact and High‐Throughput Dielectrophoretic Microfluidics

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)

Published: Jan. 18, 2024

Abstract Dielectrophoresis (DEP) particle separation has label‐free, well‐controllable, and low‐damage merits. Sidewall microelectrodes made of liquid metal alloy (LMA) inherits the additional advantage thick electrodes to generate impactful DEP force. However, existing LMA electrode‐based devices lack ability integrate large‐array in a compact footprint, severely limiting flow rate thus throughput. Herein, facile versatile method is proposed high‐density microfluidic devices, taking passive control capillary burst valves (CBVs). CBVs with carefully designed pressures are co‐designed channels, allowing self‐assembly electrode array through simple hand‐push injection. The arrayed configuration brings accumulative deflection effect. Specifically, fabricated 5000 pairs sidewall chip demonstrted achieve ten times higher throughput deflection. 5000‐electrode‐pair device applied successfully separate four mixed samples, including human peripheral blood mononuclear cells A549 70 µL min −1 . It envisioned that this work can greatly facilitate fabrication offer robust platform for applications.

Language: Английский

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Microfluidic Impedance Cytometry Enabled One‐Step Sample Preparation for Efficient Single‐Cell Mass Spectrometry

Small, Journal Year: 2024, Volume and Issue: 20(26)

Published: March 14, 2024

Abstract Single‐cell mass spectrometry (MS) is significant in biochemical analysis and holds great potential biomedical applications. Efficient sample preparation like sorting (i.e., separating target cells from the mixed population) desalting moving off non‐volatile salt solution) urgently required single‐cell MS. However, traditional methods suffer complicated operation with various apparatus, or insufficient performance. Herein, a one‐step strategy by leveraging label‐free impedance flow cytometry (IFC) based microfluidics proposed. Specifically, IFC framework to characterize sort single‐cells adopted. Simultaneously sorting, cell transferred local high‐salinity buffer MS‐compatible solution. In this way, are achieved collected can be directly fed for MS analysis. A high efficiency (>99%), cancer purity (≈87%), whole workflow of impedance‐based separation normal (MCF‐10A) (MDA‐MB‐468) verified. As standalone module, microfluidic chip compatible variety methods, envisioned provide new paradigm efficient preparation, further multi‐modal electrical metabolic) characterization single‐cells.

Language: Английский

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Impedance‐Based Multimodal Electrical‐Mechanical Intrinsic Flow Cytometry

Small, Journal Year: 2023, Volume and Issue: 19(45)

Published: July 12, 2023

Abstract Reflecting various physiological states and phenotypes of single cells, intrinsic biophysical characteristics (e.g., mechanical electrical properties) are reliable important, label‐free biomarkers for characterizing cells. However, single‐modal or properties alone not specific enough to characterize cells accurately, it has been long challenging couple the conventionally image‐based characterization impedance‐based characterization. In this work, spatial‐temporal impedance sensing signal leveraged, an multimodal electrical‐mechanical flow cytometry framework on‐the‐fly high‐dimensional measurement is proposed, that is, Young's modulus E , fluidity β radius r cytoplasm conductivity σ i membrane capacitance C sm With characterization, can better reveal difference in cell types, demonstrated by experimental results with three types cancer (HepG2, MCF‐7, MDA‐MB‐468) 93.4% classification accuracy pharmacological perturbations cytoskeleton (fixed Cytochalasin B treated cells) 95.1% accuracy. It envisioned provides a new perspective accurate single‐cell

Language: Английский

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Application of bioelectrical impedance detection techniques: cells and tissues

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 273, P. 117159 - 117159

Published: Jan. 11, 2025

Language: Английский

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Floating-Electrode-Enabled Impedance Cytometry for Single-Cell 3D Localization

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(15), P. 6374 - 6382

Published: March 30, 2023

As a label-free, low-cost, and noninvasive tool, impedance measurement has been widely used in single-cell characterization analysis. However, due to the tiny volume of cells, uncertainty spatial position microchannel will bring errors electrical parameters. To overcome issue, we designed novel microdevice configured with coplanar differential electrode structure accurately resolve single cells without constraining techniques such as additional sheath fluids or narrow microchannels. The device precisely localizes by measuring induced current generated combined action floating electrodes when flow through electrode-sensing area. was experimentally validated 6 μm yeast 10 particles, achieving localization resolution down 2.1 (about 5.3% channel width) lateral direction 1.2 5.9% height) vertical at rate μL/min. In addition, comparing it demonstrated that not only particles but also simultaneously characterizes their status properties velocity size. offers competitive configuration cytometry advantages simple structure, low cost, high throughput, promising cell thus characterization.

Language: Английский

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Non‐Invasive and Minute‐Frequency 3D Tomographic Imaging Enabling Long‐Term Spatiotemporal Observation of Single Cell Fate

Small Methods, Journal Year: 2023, Volume and Issue: 7(7)

Published: March 22, 2023

Non-invasive and rapid imaging technique at subcellular resolution is significantly important for multiple biological applications such as cell fate study. Label-free refractive-index (RI)-based 3D tomographic constitutes an excellent candidate of cellular structures, but its full potential in long-term spatiotemporal observation locked due to the lack efficient integrated system. Here, a RI system incorporating cutting-edge white light diffraction phase microscopy module with stability, acoustofluidic device roll culture single cells customized live chamber reported. Using this system, experiments are conducted 250 demonstrate identification high accuracy. Importantly, frequency-on-demand K562 MCF-7 cancer reveal different characteristics during normal growth, drug-induced apoptosis, necrosis drug-treated cells. Overall, it believed that proposed opens up new avenue visualizing intracellular structures will find many disease diagnosis nanomedicine.

Language: Английский

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Machine learning empowered multi-stress level electromechanical phenotyping for high-dimensional single cell analysis

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 225, P. 115086 - 115086

Published: Jan. 19, 2023

Language: Английский

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Performance-enhanced clogging-free viscous sheath constriction impedance flow cytometry

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(11), P. 2531 - 2539

Published: Jan. 1, 2023

As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) suffers from clogging caused by narrow microchannel as mechanical constriction (MC). Current sheath (SC) solutions lack systematic evaluation of the performance proper guidelines for fluid. Herein, we hypothesize that viscosity non-conductive liquid is key to SC, propose employ viscous in SC unlock tradeoff between sensitivity throughput, while ensuring measurement accuracy. By placing MC series same microfluidic chip, established an platform prove hypothesis. Through modeling experiments, confirmed accuracy (error < 1.60% ± 4.71%) w.r.t. MC, demonstrated PEG solution achieved improved (7.92×) signal-to-noise ratio (1.42×) measurement, with maintained free clogging. Viscous IFC also shows satisfactory ability distinguish different types cancer cells subtypes human breast cells. It envisioned paves way be really usable practice clogging-free, accurate, sensitive performance.

Language: Английский

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Evaluating the Accuracy of Impedance Flow Cytometry with Cell-Sized Liposomes

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(7), P. 2681 - 2690

Published: June 22, 2023

Electrical properties of single cells are important label-free biomarkers disease and immunity. At present, impedance flow cytometry (IFC) provides means for high throughput characterization single-cell electrical properties. However, the accuracy spherical single-shell model widely used in IFC has not been well evaluated due to lack reliable reproducible particles with true-value parameters as benchmarks. Herein, a method is proposed evaluate cell-sized unilamellar liposomes synthesized through double emulsion droplet microfluidics. The influence three key dimension (i.e., measurement channel width w, height h, electrode gap g) were experiment. It was found that relative error intrinsic measured by less than 10% when size sensing zone close particles. further reveals h greatest on accuracy, maximum can reach ∼30%. Error caused g slightly larger w. This solid guideline design system. envisioned this advance improvement accurate cells.

Language: Английский

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