Investigation of geometry-dependent sensing characteristics of microfluidic for single-cell 3D localization DOI
Tan Wang, Qiang Fang, Liang Huang

et al.

Review of Scientific Instruments, Journal Year: 2024, Volume and Issue: 95(1)

Published: Jan. 1, 2024

Flow cytometry-based measurement techniques have been widely used for single-cell characterizations, such as impedance, size, and dielectric properties. However, in the process, reliability of output signal directly affects ability microsystem to judge characteristics single cells. Here, we designed a multiple nonparallel electrode structure 3D localization. The performance structures was studied by analyzing changes electric field strength differential current. effects microchannel height, sensing distance, inclination angle, width on signals are investigated current change cells passing from center microchannel. numerical simulation results indicate that, when height is 20 µm, distance electrodes 100 angle 30°, optimal quality can be obtained. Reducing flow channel shortening spacing significantly improve amplitude. When intensity increases 80% than that 30 µm. induced with µm 42% higher 120 We analyzed presence independent adherent detection area demonstrated through caused multi-cells superimposed signals. same volume an ellipticity 1 49% lower 4. Based investigation, expect geometry design will aid development better cell impedance cytometry microsystems.

Language: Английский

Capillarity Enabled Large‐Array Liquid Metal Electrodes for Compact and High‐Throughput Dielectrophoretic Microfluidics DOI
Huichao Chai,

Junwen Zhu,

Yongxiang Feng

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)

Published: Jan. 18, 2024

Abstract Dielectrophoresis (DEP) particle separation has label‐free, well‐controllable, and low‐damage merits. Sidewall microelectrodes made of liquid metal alloy (LMA) inherits the additional advantage thick electrodes to generate impactful DEP force. However, existing LMA electrode‐based devices lack ability integrate large‐array in a compact footprint, severely limiting flow rate thus throughput. Herein, facile versatile method is proposed high‐density microfluidic devices, taking passive control capillary burst valves (CBVs). CBVs with carefully designed pressures are co‐designed channels, allowing self‐assembly electrode array through simple hand‐push injection. The arrayed configuration brings accumulative deflection effect. Specifically, fabricated 5000 pairs sidewall chip demonstrted achieve ten times higher throughput deflection. 5000‐electrode‐pair device applied successfully separate four mixed samples, including human peripheral blood mononuclear cells A549 70 µL min −1 . It envisioned that this work can greatly facilitate fabrication offer robust platform for applications.

Language: Английский

Citations

37
Microfluidic Impedance Cytometry Enabled One‐Step Sample Preparation for Efficient Single‐Cell Mass Spectrometry DOI

Junwen Zhu,

Siyuan Pan, Huichao Chai

et al.

Small, Journal Year: 2024, Volume and Issue: 20(26)

Published: March 14, 2024

Abstract Single‐cell mass spectrometry (MS) is significant in biochemical analysis and holds great potential biomedical applications. Efficient sample preparation like sorting (i.e., separating target cells from the mixed population) desalting moving off non‐volatile salt solution) urgently required single‐cell MS. However, traditional methods suffer complicated operation with various apparatus, or insufficient performance. Herein, a one‐step strategy by leveraging label‐free impedance flow cytometry (IFC) based microfluidics proposed. Specifically, IFC framework to characterize sort single‐cells adopted. Simultaneously sorting, cell transferred local high‐salinity buffer MS‐compatible solution. In this way, are achieved collected can be directly fed for MS analysis. A high efficiency (>99%), cancer purity (≈87%), whole workflow of impedance‐based separation normal (MCF‐10A) (MDA‐MB‐468) verified. As standalone module, microfluidic chip compatible variety methods, envisioned provide new paradigm efficient preparation, further multi‐modal electrical metabolic) characterization single‐cells.

Language: Английский

Citations

35
Impedance‐Based Multimodal Electrical‐Mechanical Intrinsic Flow Cytometry DOI
Yongxiang Feng,

Junwen Zhu,

Huichao Chai

et al.

Small, Journal Year: 2023, Volume and Issue: 19(45)

Published: July 12, 2023

Abstract Reflecting various physiological states and phenotypes of single cells, intrinsic biophysical characteristics (e.g., mechanical electrical properties) are reliable important, label‐free biomarkers for characterizing cells. However, single‐modal or properties alone not specific enough to characterize cells accurately, it has been long challenging couple the conventionally image‐based characterization impedance‐based characterization. In this work, spatial‐temporal impedance sensing signal leveraged, an multimodal electrical‐mechanical flow cytometry framework on‐the‐fly high‐dimensional measurement is proposed, that is, Young's modulus E , fluidity β radius r cytoplasm conductivity σ i membrane capacitance C sm With characterization, can better reveal difference in cell types, demonstrated by experimental results with three types cancer (HepG2, MCF‐7, MDA‐MB‐468) 93.4% classification accuracy pharmacological perturbations cytoskeleton (fixed Cytochalasin B treated cells) 95.1% accuracy. It envisioned provides a new perspective accurate single‐cell

Language: Английский

Citations

32
Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances DOI Creative Commons
Fatemeh Dadkhah Tehrani, Michael O’Toole, David J. Collins

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Integration of low-frequency electrical impedance and broadband electromagnetic sensing with microfluidic devices enables high-throughput analysis cell size, membrane properties, intracellular characteristics.

Language: Английский

Citations

1
Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae DOI Creative Commons
Zhongyi Guo,

Nana Ai,

Wei Ge

et al.

Microsystems & Nanoengineering, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 29, 2024

This paper presents the design of a vision-based automated robotic microinjection system for batch injection both zebrafish embryos and larvae. A novel visual recognition algorithm based on an automatic threshold excessive dilatation is introduced to accurately identify center larval yolks. corresponding software developed using producer-consumer model as framework structure, friendly user interface designed allow operators choose from range desired functions according their different needs. In addition, microstructural agarose device fabricated simultaneously immobilize mixed batches Moreover, prototype by integrating hardware devices with algorithms. An experimental study conducted verify performance system. The results show that reported can larvae efficiently complete tasks mixtures success rate 92.05% in 13.88 s per sample. Compared manual existing systems, proposed demonstrates merits versatility, excellent efficiency, high rate, survival sufficient stability.

Language: Английский

Citations

6
Convolutional Neural Network-Driven Impedance Flow Cytometry for Accurate Bacterial Differentiation DOI

Shuaihua Zhang,

Ziyu Han,

H. Jerry Qi

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4419 - 4429

Published: March 6, 2024

Impedance flow cytometry (IFC) has been demonstrated to be an efficient tool for label-free bacterial investigation obtain the electrical properties in real time. However, accurate differentiation of different species bacteria by IFC technology remains a challenge owing insignificant differences data. Here, we developed convolutional neural networks (ConvNet) deep learning approach enhance accuracy and efficiency toward distinguishing various bacteria. First, more than 1 million sets impedance data (comprising 42 characteristic features each set) groups were trained ConvNet model. To improve analysis, Spearman correlation coefficient mean decrease random forest algorithm introduced eliminate feature interaction extract opacity related wall membrane structure as predominant differentiation. Moreover, 25 optimized selected with accuracies >96% three (bacilli, cocci, vibrio) >95% two bacilli (Escherichia coli Salmonella enteritidis), compared machine algorithms (complex tree, linear discriminant, K-nearest neighbor algorithms) maximum 76.4%. Furthermore, was achieved on spiked samples mixing ratios. The proposed learning-assisted analysis method exhibits advantages analyzing huge number capacity extracting within multicomponent information will bring about progress advances fields both biosensing analysis.

Language: Английский

Citations

6
Label-Free Differentiation of Living versus Dead Single Yeast Cells using Broadband Electrical Impedance Spectroscopy DOI Creative Commons
Amirhossein Favakeh,

Amir Mokhtare,

Mohammad Javad Asadi

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The use of the intrinsic electrical properties a single cell by broadband impedance spectroscopy (EIS) as label-free and non-invasive method to monitor cellular intracellular features is an emerging field. Here, we present novel EIS-based sheathless microfluidic platform with integrated coplanar waveguide probe interior cell. This allows for precise single-cell trapping dielectrophoresis, hydrodynamic focusing, sensing trapped We measured characteristics Schizosaccharomyces pombe (fission) yeast frequency sweep (30 kHz 6GHz) in stagnant sucrose solution using two-port scattering (S) parameters. measurements revealed clear distinction between cytoplasm live versus dead cells at 3 GHz. could provide real-time monitoring responses chemical physical antagonists diagnostic purposes.

Language: Английский

Citations

0
Microfluidic Biosensors for the Detection of Motile Plant Zoospores DOI Creative Commons
Peikai Zhang, David E. Williams, Lyndon Scott Stephens

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 131 - 131

Published: Feb. 21, 2025

Plant pathogen zoospores play a vital role in the transmission of several significant plant diseases, with their early detection being important for effective management. Current methods involve labour-intensive specimen collection and laboratory testing, lacking real-time feedback capabilities. Methods that can be deployed field remotely addressed are required. In this study, we have developed an innovative zoospore-sensing device by combining microfluidic sampling system cytometer incorporating chemotactic response as means to selectively detect motile spores. Spores Phytophthora cactorum were guided swim up channel following gradient attractant. They then detected transient change impedance when they passed between pair electrodes. Single-zoospore was demonstrated signal-to-noise ratios ~17 carrying flow used ~5.9 induced into attractants. This work provides solution selective, sensitive zoospores. It has great potential further portable, addressable, low-cost sensing system, offering tool applications.

Language: Английский

Citations

0
Materials evaluation using cell-sized liposomes DOI
Tsuyoshi Yoda

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(32), P. 5509 - 5518

Published: Jan. 1, 2024

This review introduces various studies on cell-sized liposomes, which facilitate observation for the rapid detection and analysis of diverse materials based their size, dynamic shape changes, phase separation, providing valuable insights.

Language: Английский

Citations

1
Waste Nitrogen Upcycling to Amino Acids during Anaerobic Fermentation on Biochar: An Active Strategy for Regulating Metabolic Reducing Power DOI
Yanfei Tang, Eakalak Khan, Daniel C.W. Tsang

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(45), P. 20060 - 20072

Published: Nov. 1, 2024

This study proposes a novel strategy that utilizes biochar (BC) during anaerobic fermentation (AF) to generate amino acids (AAs) toward nitrogen upcycling. The BC, pyrolyzed at 800 °C (BC800) enhance graphite structures and electron-accepting sites, effectively addresses issues related biosynthetic reducing power nicotinamide adenine dinucleotide phosphate insufficiency by altering cellular conditions alleviates feedback inhibition through the immobilization of end products. process establishes unique microbial signaling energy networks, with

Language: Английский

Citations

1