Langmuir,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Copolymer
nanovesicles
are
used
extensively
in
chemical
processes
and
biomedical
applications
which
they
subjected
to
dynamic
flow
environments.
Flow-induced
vesicle
deformation,
fragmentation,
reorganization
modify
the
energetic
(e.g.,
polymer-solvent
interfacial
area)
entropic
copolymer
chain
configuration)
contributions
solution
free
energy.
Equilibration
of
a
deformed
morphology
by
cessation
could
reorganize
system
into
self-assembled
state,
is
different
from
parent
structure
through
local
energy
minimization
pathway.
We
perform
nonequilibrium
molecular
dynamics
simulations
investigate
evolution
uniform
shear
unilamellar
nanovesicle
formed
self-assembly
amphiphilic
triblock
copolymers
an
aqueous
solution.
Flow
strength
characterized
Weissenberg
number
Advanced Science,
Journal Year:
2023,
Volume and Issue:
11(8)
Published: Nov. 20, 2023
The
exponential
growth
of
research
on
artificial
cells
and
organelles
underscores
their
potential
as
tools
to
advance
the
understanding
fundamental
biological
processes.
bottom-up
construction
from
a
variety
building
blocks
at
micro-
nanoscale,
in
combination
with
biomolecules
is
key
developing
cells.
In
this
review,
are
focused
upon
based
compartments
where
polymers
main
constituent
assembly.
Polymers
particular
interest
due
incredible
chemical
advantage
tuning
properties
functionality
assemblies.
First,
architectures
nanoscale
polymer
assemblies
introduced
then
usage
elaborated
upon.
Different
membrane-bound
membrane-less
supramolecular
structures
how
they
combine
into
advanced
synthetic
presented.
Then,
functional
aspects
explored,
addressing
giant
mimic
cellular
Finally,
communicate
surrounding
each
other
such
adapt
an
ever-changing
environment
achieve
collective
behavior
steppingstone
toward
tissues,
taken
look
at.
Engineering
highly
controllable
programmable
features
open
new
avenues
for
development
sophisticated
multifunctional
systems.
ACS Applied Engineering Materials,
Journal Year:
2024,
Volume and Issue:
2(12), P. 2758 - 2770
Published: March 21, 2024
This
review
explores
the
cutting-edge
development
of
multicompartment
synthetic
vesicles
designed
for
artificial
cell
applications,
drawing
inspiration
from
complex
compartmentalization
inherent
in
living
cells.
It
delves
into
recent
advancements
engineering
equipped
with
both
membranous
and
membraneless
organelles
(vesicles-in-vesicles
coacervates-in-vesicles),
offering
a
detailed
examination
methodologies
materials
employed.
paper
highlights
critical
role
these
simulating
cellular
microenvironments
functions,
facilitating
spatial
temporal
segregation
biochemical
processes,
such
as
signal
transduction,
gene
expression,
ATP
synthesis,
energy
production.
Moreover,
this
outlines
potential
future
directions,
emphasizing
importance
evolution
cells
focus
on
their
application
creating
more
sophisticated
biomimetic
systems.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(37), P. 20319 - 20327
Published: Sept. 7, 2023
The
bottom-up
assembly
of
biomimetic
multicompartmentalized
microreactors
for
use
in
continuous
flow
catalysis
remains
a
grand
challenge
because
the
structural
instability
or
absence
liquid
microenvironments
to
host
biocatalysts
existing
systems.
Here,
we
address
this
using
strategy
that
combines
stepwise
Pickering
emulsification
with
interface-confined
cross-linking.
Our
allows
fabrication
robust
liquid-containing
(MLMs),
whose
interior
architectures
can
be
exquisitely
tuned
fashion.
With
strategy,
enzymes
and
metal
catalysts
separately
confined
distinct
subcompartments
MLMs
processing
biocatalysis
chemo-enzymatic
cascade
reactions.
As
exemplified
by
enzyme-catalyzed
kinetic
resolution
racemic
alcohols,
our
systems
exhibit
durability
2000
h
99%
enantioselectivity.
Another
Pd-enzyme-cocatalyzed
dynamic
amines
further
demonstrates
versatility
long-term
operational
stability
catalysis.
This
study
opens
up
new
way
design
efficient
multicompartmental
practical
applications.
Advanced Materials Technologies,
Journal Year:
2024,
Volume and Issue:
9(18)
Published: May 1, 2024
Abstract
Designing
and
assembling
artificial
cells
(ACs)
is
a
core
direction
in
bottom‐up
synthetic
biology.
Here,
the
advancements
past
3
years
engineering
ACs
with
focus
on
compartmentalization
surface
modifications
aim
for
their
integration
semi‐synthetic
tissue
are
outlined.
Compartmentalization
vesicles,
coacervates
hydrogels
discussed
encapsulated
catalysis
or
cytoskeleton
formation
including
use
of
components
mammalian
to
increase
ACs’
complexity.
Following
on,
modification
reviewed
due
its
relevance
when
into
goal.
Finally,
interaction
cellular
communication
fabrication
toward
therapeutic
opportunities
outlined,
before
short
perspective
provided.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 10, 2025
Abstract
Multicellular
organisms
have
hierarchical
structures
where
multiple
cells
collectively
form
tissues
with
complex
3D
architectures
and
exhibit
higher‐order
functions.
Inspired
by
this,
to
date,
protocell
models
been
assembled
tissue‐like
termed
prototissues.
Despite
recent
advances
in
this
research
area,
the
programmed
assembly
of
protocells
into
prototissue
fibers
emergent
functions
still
represents
a
significant
challenge.
The
possibility
assembling
will
open
up
way
novel
type
subunit
capable
unprecedented
soft
functional
materials
tunable
architectures,
modular
distributed
functionalities.
Herein,
first
method
fabricate
freestanding
vesicle‐based
controlled
lengths
diameters
is
devised.
Importantly,
it
also
shown
that
can
be
composed
different
specialized
modules
that,
for
example,
endow
fiber
magnetotaxis
capabilities,
or
work
synergistically
take
an
input
diffusible
chemical
signals
transduce
readable
fluorescent
output
through
hosted
enzyme
cascade
reaction.
Overall,
addresses
important
challenge
engineering
find
applications
bio‐printing,
tissue
engineering,
robotics
as
next‐generation
bioinspired
materials.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 20, 2025
The
dynamic
study
of
coacervates
in
vitro
contributes
our
understanding
phase
separation
mechanisms
cells
due
to
complex
intracellular
physiology.
However,
current
researches
mainly
involve
the
use
exogenous
auxiliary
agents
form
multi-compartmental
with
short-term
stability.
Herein,
we
report
endogenous
self-organizing
multi-component
(HA/PDDA/BSA/DMAEMA)
induced
by
a
stimulation
process
protein-mediated
photopolymerization.
As
polymerization
proceeds,
cycled
structural
evolution
and
maturation
from
coacervate
droplets
into
coacervates,
vesicles
are
revealed,
which
driven
electrostatic
interaction
osmotic
pressure
difference
supported
thermodynamic
control.
Specially,
regulating
light
time,
type
can
be
widely
obtained
high
stability
over
300
days.
Being
promising
artificial
cell
model,
it
shows
special
characteristic
compartmentalized
encapsulation
substrates,
efficiently
improving
enzymatic
interfacial
catalytic
efficiency
organelle-like
communication.
Our
holds
great
potential
for
advancing
mechanism
membraneless
organelles
provides
an
instructive
technique
constructing
long-term
Coacervate
dynamics
studied
aid
cells,
but
obtaining
sufficient
challenging.
Here,
authors
development
that
self-organise
protein
mediated
photopolymerisation,
stable
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
Abstract
The
integration
of
artificial
and
mammalian
cells
into
semi‐synthetic
aggregates
remains
a
challenge
in
bottom‐up
synthetic
biology.
Here,
the
fabrication
cell
membrane
vesicles
(CMV)
from
HepG2
their
use
as
coating
for
alginate
microgels
to
produce
camouflaged
(ACs)
is
demonstrated.
These
ACs
are
used
assembly
either
or
aggregates.
In
first
case,
predator‐defendant
liver‐like
investigated,
showing
promising
initial
steps
toward
complex
other
show
enhanced
with
cells.
encapsulation
reactive
oxygen
species
(ROS)
scavenger
enzyme
shows
protection
against
tert
‐butyl
hydroperoxide
terms
viability,
proliferation,
mitochondrial
health
Taken
together,
this
effort
substantial
step
forward
combining
same
aggregate
where
latter
act
support
units.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 24, 2025
Abstract
Droplet‐based
microreactors
hold
great
promise
for
enzymatic
reactions,
but
their
efficiency
is
often
hindered
by
static
operation
and
poor
mass
transfer.
This
study
tackles
these
limitations
through
the
innovative
application
of
dynamic
rotating
magnetic
liquid
marbles
(LMs)
stabilized
proteinaceous
colloidosomes.
The
colloidosomes
feature
an
outer
layer
densely‐packed
hydrophobic
silica
nanoparticles
inner
zein
that
integrates
particles
enzymes,
enhancing
hydrophobicity,
responsiveness,
enzyme
immobilization.
As
proof
concept,
glucose
oxidase
(GOx)
encapsulated
within
horseradish
peroxidase
(HRP)
inside
LMs,
respectively.
results
demonstrate
LMs
significantly
accelerated
cascade
reactions
when
exposed
to
external
field.
Moreover,
applying
this
approach
biphasic
systems,
positioning
at
water/toluene
interface
yields
a
2.2‐fold
increase
in
conversion
rate
compared
as
shown
lipase‐driven
esterification
reactions.
advancement
not
only
broadens
potential
applications
also
offers
promising
strategy
catalytic
processes
heterogeneous
systems.
Small,
Journal Year:
2024,
Volume and Issue:
20(30)
Published: Feb. 28, 2024
Abstract
Multicellular
organisms
demonstrate
a
hierarchical
organization
where
multiple
cells
collectively
form
tissues,
thereby
enabling
higher‐order
cooperative
functionalities
beyond
the
capabilities
of
individual
cells.
Drawing
inspiration
from
this
biological
organization,
assemblies
protocells
are
developed
to
create
novel
functional
materials
with
emergent
functionalities.
This
paper
presents
new
artificial
tissues
derived
vesicles,
which
serve
as
protocellular
models.
These
formed
and
manipulated
through
non‐covalent
interactions
triggered
by
salt
bridge.
Exhibiting
pH‐sensitive
reversible
formation
destruction
under
neutral
conditions,
these
vesicle
three
distinct
functionalities:
transportation
large
cargoes,
photo‐induced
contractions,
enhanced
survivability
against
external
threats.
The
rapid
assembly
disassembly
in
response
pH
variations
enable
controlled
mechanical
task
performance.
Additionally,
self‐healing
property
indicates
robustness
damage.
research
suggests
that
vesicles
can
detect
specific
environments
spontaneously
assemble
into
advanced
leads
possibility
developing
intelligent
high
environmental
specificity,
particularly
for
applications
soft
robotics.
