Metabolites,
Journal Year:
2024,
Volume and Issue:
14(4), P. 236 - 236
Published: April 19, 2024
This
study
evaluated
effects
of
high
starch
(20%)
on
hematological
variations,
glucose
and
lipid
metabolism,
antioxidant
ability,
inflammatory
responses,
histopathological
lesions
in
largemouth
bass.
Results
showed
hepatic
crude
triacylglycerol
(TAG)
contents
were
notably
increased
fish
fed
starch.
High
could
increase
counts
neutrophils,
lymphocytes,
monocytes,
eosinophils,
basophils
serum
TAG,
TBA,
BUN,
LEP
(
Environment International,
Journal Year:
2022,
Volume and Issue:
166, P. 107351 - 107351
Published: June 15, 2022
Perfluorooctane
sulfonate
(PFOS)
has
been
reported
to
induce
hepatotoxicity
in
wildlife
and
humans.
Novel
PFOS
alternatives
have
widely
used
following
restrictions
on
PFOS,
but
little
is
known
about
their
potential
toxicity.
Here,
the
first
comprehensive
investigation
chronic
underlying
molecular
mechanisms
of
6:2Cl-PFESA
(F-53B),
sodium
p-perfluorous
nonenoxybenzene
(OBS)
was
carried
out
adult
zebrafish
through
a
histopathological
examination,
biochemical
measurement,
multi-omics
analysis.
its
caused
changes
liver
histopathology
function
indices
order
F-53B
>
OBS,
which
consistent
with
concentration
liver.
In
silico
modeling
transcriptional
profiles
suggested
that
aberrant
hepatic
lipid
metabolism
induced
by
initiated
action
peroxisome
proliferator-activated
receptor
γ
(PPARγ),
triggered
downstream
genes
transcription
led
an
imbalance
between
synthesis
expenditure.
Gut
microbiome
analysis
provided
another
novel
mechanistic
perspective
abundance
Legionella,
Ralstonia,
Brevundimonas,
Alphaproteobacteria,
Plesiomonas,
Hyphomicrobium
might
link
alterations
PPAR
pathway
based
significant
correlation.
This
study
provides
insight
into
highlights
need
for
concern
environmental
exposure
risks.
Frontiers in Physiology,
Journal Year:
2022,
Volume and Issue:
13
Published: May 23, 2022
Due
to
its
special
flavour
and
cheapness,
starch
is
a
source
of
nutrition
for
humans
most
animals,
some
whom
even
prefer
consume
large
amounts
starchy
foods.
However,
the
use
by
carnivorous
fish
limited
excessive
intake
can
lead
liver
damage,
but
mechanism
damage
not
clear.
Therefore,
in
this
study,
two
isonitrogenous
isolipid
semi-pure
diets,
Z
diet
(0%
starch)
G
(22%
starch),
were
formulated,
respectively.
The
largemouth
bass
(
M
.
salmoides
)
cultured
fiberglass
tanks
randomly
divided
into
groups
fed
diets
45
days.
Blood
collected
on
day
30
enzymology,
histopathology,
ultramicropathology,
flow
cytometry,
transcriptomics
investigate
high
mechanism.
results
showed
that
affect
growth
performance
bass.
caused
whitening
an
increase
hepatopancreas
index
(HSI),
aspartate
aminotransferase
(AST),
alanine
(ALT)
serum.
Histopathological
observations
led
severe
vacuolisation,
congestion,
moderate
necrotizing
hepatitis
liver.
significant
postprandial
blood
glucose
insulin
serum
bass,
promoting
synthesis
accumulation
hepatic
glycogen
liver,
leading
loss
hepatocyte
organelles
inducing
fibrosis.
Meanwhile,
induced
production
oxidative
stress
promoted
apoptosis
necrosis
hepatocytes.
Transcriptome
analysis
revealed
there
10,927
2,656
unique
genes
groups,
KEGG
enrichment
19
pathways
significantly
enriched,
including
those
related
metabolism
cell
survival.
Network
mapping
based
differential
expressing
emergence
regulatory
network
dominated
PI3K/Akt
signaling
pathway.
This
indicated
signalling
pathway
plays
very
important
role
process,
regulating
injury
starch.
Our
provide
reference
starch,
could
be
potential
therapeutic
target
Aquaculture Nutrition,
Journal Year:
2022,
Volume and Issue:
2022, P. 1 - 15
Published: April 5, 2022
A
56-day
feeding
experiment
was
conducted
to
determine
the
dietary
lysine
requirement
of
juvenile
largemouth
bass
(Micropterus
salmoides)
and
investigate
effects
on
growth,
whole-body
composition,
hepatic
gene
expression
related
glycolipid
metabolism
via
AKT/FoxO1
pathway.
The
juveniles
(
g)
were
fed
six
graded
levels
(2.11%
(control),
2.56%,
2.92%,
3.33%,
3.68%,
4.09%,
dry
diet).
results
showed
that
3.33%
level
significantly
increased
final
body
weight
(FBW),
gain
rate
(WGR),
specific
growth
(SGR)
improved
feed
conversion
ratio
(FCR)
compared
with
control
group.
composition
not
affected
by
levels,
while
lowest
lipid
contents
found
in
2.92%
groups.
Regarding
metabolism,
group,
protein
kinase
B
(AKT)
inhibited
forkhead
box
O1
(FoxO1),
thus
upregulated
pyruvate
(PK)
mRNA
enhance
glycolysis.
Furthermore,
sterol-regulatory
element
binding
protein-1c
(SREBP1c)
peroxisome
proliferator-activated
receptor-γ
(PPAR-γ)
downregulated
lysine,
which
caused
downregulation
synthesis-related
genes
acetyl-CoA
carboxylase-1
(ACC)
stearyl-CoA
desaturase
(SCD)
mRNA.
In
addition,
promoted
lipolysis-related
receptor-α
(PPAR-α)
carnitine
palmitoyl
transferase-1
(CPT1).
According
quadratic
regression
analysis
based
FCR
SGR
values,
optimal
estimated
be
3.03%
3.07%
diet
(6.39%
6.48%
protein),
respectively.
