Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 26, 2025
Abstract
Insect
development
is
primarily
controlled
by
juvenile
hormone
(JH)
and
20-hydroxyecdysone
(20E),
which
regulate
gene
cascades
leading
to
changes
in
phenotype,
physiology,
behavior.
Besides
these
hormones,
microRNAs
play
a
crucial
role
insect
regulating
expression
at
the
post-transcriptional
level.
To
advance
molecular
understanding
of
holometabolous
developmental
events,
we
investigate
pupal
phase
honeybee,
Apis
mellifera
.
In
this
study,
assessed
profiles
genes
components
JH
20E
–
Usp,
ftz-f1,
EcR,
Met,
Chd64,
InR-2,
Kr-h1
Tai
as
well
miRNA-34
miRNA-281
during
A.
We
then
analyzed
impact
treatments
on
their
putative
regulators,
microRNAs.
Overall,
selected
miRNAs
remained
stable
or
were
downregulated
following
treatment,
while
with
JH,
upregulated
most
our
candidate
Notably,
profile
Met
,
an
intracellular
receptor
showed
strong
correlation
fluctuations
titers
development.
Furthermore,
computational
analysis,
followed
experimental
assays,
points
both
miR-34
miR-281
potential
regulators
This
study
paves
way
for
better
how
hormones
interact
(miR-34
miR-281)
honeybees,
elucidating
piece
complex
network
interactions.
Frontiers in Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
8
Published: Jan. 28, 2021
Vitellogenesis
is
pre-requisite
to
insect
egg
production
and
embryonic
development
after
oviposition.
During
vitellogenesis,
the
yolk
protein
precursor
vitellogenin
(Vg)
mainly
synthesized
in
fat
body,
transported
by
hemolymph
through
intercellular
spaces
(known
as
patency)
follicular
epithelium
reach
membrane
of
maturing
oocytes,
sequestered
into
oocytes
via
receptor-mediated
endocytosis.
Insect
vitellogenesis
governed
two
critical
hormones,
sesquiterpenoid
juvenile
hormone
(JH)
ecdysteriod
20-hydroxyecdysone
(20E).
JH
acts
principal
gonadotropic
stimulate
basal
hemimetabolous
most
holometabolous
insects.
20E
for
some
hymenopterans,
lepidopterans
dipterans.
Furthermore,
microRNA
(miRNA)
nutritional
(amino
acid/Target
Rapamycin
insulin)
pathways
interplay
with
signaling
cascades
control
vitellogenesis.
Revealing
regulatory
mechanisms
underlying
understanding
reproduction
helpful
developing
new
strategies
pest
control.
Here,
we
outline
recent
research
progress
molecular
action
along
role
miRNA
sensor
regulating
We
highlight
advancements
coordination
hormone,
pathways.
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(39)
Published: Sept. 20, 2021
It
is
well
documented
that
the
juvenile
hormone
(JH)
can
function
as
a
gonadotropic
stimulates
vitellogenesis
by
activating
production
and
uptake
of
vitellogenin
in
insects.
Here,
we
describe
phenotype
associated
with
mutations
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(11), P. 9161 - 9161
Published: May 23, 2023
The
spotted
bollworm
Earias
vittella
(Lepidoptera:
Nolidae)
is
a
polyphagous
pest
with
enormous
economic
significance,
primarily
affecting
cotton
and
okra.
However,
the
lack
of
gene
sequence
information
on
this
has
significant
constraint
molecular
investigations
formulation
superior
management
strategies.
An
RNA-seq-based
transcriptome
study
was
conducted
to
alleviate
such
limitations,
de
novo
assembly
performed
obtain
transcript
sequences
pest.
Reference
identification
across
E.
developmental
stages
RNAi
treatments
were
using
its
information,
which
resulted
in
identifying
transcription
elongation
factor
(TEF),
V-type
proton
ATPase
(V-ATPase),
Glyceraldehyde
-3-phosphate
dehydrogenase
(GAPDH)
as
most
suitable
reference
genes
for
normalization
RT-qPCR-based
expression
studies.
present
also
identified
important
developmental,
pathway,
target
life-stage
analysis
RT-qPCR
select
optimal
targets
RNAi.
We
found
that
naked
dsRNA
degradation
hemolymph
primary
reason
poor
A
total
six
including
Juvenile
hormone
methyl
transferase
(JHAMT),
Chitin
synthase
(CHS),
Aminopeptidase
(AMN),
Cadherin
(CAD),
Alpha-amylase
(AMY),
(V-ATPase)
selected
knocked
down
significantly
three
different
nanoparticles
encapsulated
conjugates,
i.e.,
Chitosan-dsRNA,
carbon
quantum
dots-dsRNA
(CQD-dsRNA),
Lipofectamine-dsRNA
conjugate.
These
results
demonstrate
feeding
nanoparticle-shielded
silences
suggests
nanoparticle-based
can
efficiently
manage
Frontiers in Genetics,
Journal Year:
2020,
Volume and Issue:
11
Published: Sept. 10, 2020
Insects
are
arguably
the
most
successful
group
of
animals
in
world
terms
both
species
numbers
and
diverse
habitats.
The
sesquiterpenoids
juvenile
hormone,
methyl
farnesoate
farnesoic
acid
well
known
to
regulate
metamorphosis,
reproduction,
sexual
dimorphism,
eusociality,
defense
insects.
Nevertheless,
different
insects
have
evolved
with
sesquiterpenoid
biosynthetic
pathway
as
products.
On
other
hand,
noncoding
RNAs
such
microRNAs
been
implicated
regulation
many
important
biological
processes,
recently
explored
production.
In
this
review,
we
summarise
latest
findings
on
diversity
reported
groups
insects,
recent
advancements
understanding
production
by
microRNAs.
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(37)
Published: Sept. 7, 2021
Significance
During
vitellogenesis
of
oviparous
animals,
vitellogenin
receptor
(VgR)–mediated
endocytosis
is
crucial
for
the
incorporation
yolk
proteins
into
oocytes.
VgR
also
serves
as
a
gatekeeper
vertical
transmission
pathogenic
microbes
and
symbionts.
Juvenile
hormone
(JH)
acts
gonadotrophic
stimulates
this
process
in
many
insect
species,
but
regulatory
mechanisms
remain
elusive.
We
find
that
JH
activates
GPCR-PLC-PKC-ι
cascade
phosphorylates
an
EGF-precursor
homology
domain.
Endosomal
acidification
leads
to
dephosphorylation.
Phosphorylation
essential
translocate
from
oocyte
cytoplasm
membrane
bind
with
on
internalize
Moreover,
JH-promoted
phosphorylation
intracellular
recycling
deposition
evolutionarily
conserved
across
divergent
orders.
Journal of Nanobiotechnology,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Dec. 8, 2022
Abstract
Background
Nanomaterials
are
widely
used
as
pesticide
adjuvants
to
increase
efficiency
and
minimize
environmental
pollution.
But
it
is
increasingly
recognized
that
nanocarrier
a
double-edged
sword,
nanoparticles
emerging
new
pollutants.
This
study
aimed
determine
the
biotoxicity
of
applied
star
polycation
(SPc)
using
Drosophila
melanogaster
,
fruit
fly,
an
in
vivo
model.
Results
The
lethal
concentration
50
(LC
)
value
SPc
was
identified
2.14
g/L
toward
third-instar
larvae
26.33
for
adults.
Chronic
exposure
sub
(1
g/L)
larval
stage
showed
long-lasting
adverse
effects
on
key
life
history
traits.
Exposure
at
adversely
impacted
lifespan,
fertility,
climbing
ability
well
stresses
resistance
emerged
RNA-sequencing
analysis
found
resulted
aberrant
expression
genes
involved
metabolism,
innate
immunity,
stress
response
hormone
production
larvae.
Orally
administrated
were
mainly
accumulated
intestine
cells,
while
systemic
responses
observed.
Conclusions
These
findings
indicate
hazardous
flies
multiple
levels,
which
could
help
us
develop
guidelines
further
large-scale
application.
PLoS Genetics,
Journal Year:
2022,
Volume and Issue:
18(9), P. e1010411 - e1010411
Published: Sept. 16, 2022
Fecundity
is
arguably
one
of
the
most
important
life
history
traits,
as
it
closely
tied
to
fitness.
Most
arthropods
are
recognized
for
their
extreme
reproductive
capacity.
For
example,
a
single
female
oriental
fruit
fly
Bactrocera
dorsalis
,
highly
invasive
species
that
destructive
agricultural
pests
worldwide,
can
lay
more
than
3000
eggs
during
its
span.
The
ovary
crucial
insect
reproduction
and
development
requires
further
investigation
at
molecular
level.
We
report
here
miR-309a
regulator
ovarian
in
B
.
Our
bioinformatics
studies
have
revealed
binds
transcription
factor
pannier
(
GATA-binding
A
/
pnr
),
this
activates
yolk
vitellogenin
2
Vg
)
receptor
VgR
advancing
development.
show
under
control
juvenile
hormone
(JH)
independent
from
20-hydroxyecdysone.
Thus,
we
identified
JH-controlled
miR-309a/
axis
regulates
Vg2
This
study
has
enhanced
our
understanding
mechanisms
governing
reproduction.
It
provides
background
identifying
targets
controlling
Dipteran
pests.
