This
PhD
thesis,
entitled
"Holographic
biosensors
made
of
DNA-functionalised
hydrogels
for
in
vitro
diagnostics",
focuses
on
the
development
an
analyte-sensitive
hydrogel,
functionalised
with
oligonucletide
probes,
a
diffractive
structure
as
optical
transducer
diagnostic
applications.The
first
chapter
includes
overview
different
concepts
related
to
biosensing,
recent
developments
diagnostics
market
and,
particular,
DNA
biosensors.In
addition,
synthesis
and
characterization
hydrogels,
their
role
support
matrix
immobilization
strategies
are
presented.Finally,
basic
holography
new
detection
strategy
diffraction
gratings
biosensing
explained.Next,
Chapter
2,
objectives
this
project
discussed.The
aim
research
is
LIST
OF
PUBBLICATIONS
Molecules,
Journal Year:
2023,
Volume and Issue:
28(3), P. 1083 - 1083
Published: Jan. 21, 2023
The
demand
for
creation
of
protein
diversity
and
regulation
function
through
native
modification
post-translational
has
ignited
the
development
selective
chemical
methods
peptides
proteins.
Chemical
bioconjugation
offers
functionalization
providing
bioconjugates
with
desired
properties
functions
diverse
applications
in
biology,
medicine,
biomaterials.
amino
group
existing
at
lysine
residue
N-terminus
proteins
been
extensively
studied
because
its
good
nucleophilicity
high
surface
exposure.
Herein,
we
review
groups
on
featuring
excellent
selectivity,
mild
reaction
conditions,
short
time,
conversion,
biocompatibility,
preservation
integrity.
This
is
organized
based
chemoselectivity
site-selectivity
reagents
to
acid
residues
aiming
provide
guidance
selection
appropriate
methods.
The Chemical Record,
Journal Year:
2023,
Volume and Issue:
23(9)
Published: June 2, 2023
Abstract
Molecules
featuring
fluorine‐containing
functional
groups
exhibit
outstanding
properties
with
high
density,
low
sensitivity,
excellent
thermal
stability,
and
good
energetic
performance
due
to
the
strong
electron‐withdrawing
ability
density
of
fluorine.
Hence,
they
play
a
pivotal
role
in
field
materials.
In
light
current
theoretical
experimental
reports,
this
review
systematically
focuses
on
three
types
materials
possessing
F‐
NF
2
‐
substituted
trinitromethyl
(C(NO
)
F,
C(NO
),
trifluoromethyl
group
(CF
3
difluoroamino
pentafluorosulfone
(NF
,
SF
5
investigates
synthetic
methods,
physicochemical
parameters,
each.
The
incorporation
moieties
is
critical
for
development
novel
energy
materials,
rapidly
being
adopted
design
International Journal of Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
653, P. 123864 - 123864
Published: Feb. 2, 2024
Gene
therapy
can
potentially
treat
a
great
number
of
diseases,
from
cancer
to
rare
genetic
disorders.
Very
recently,
the
development
and
emergency
approval
nucleic
acid-based
COVID-19
vaccines
confirmed
its
strength
versatility.
However,
gene
encounters
limitations
due
lack
suitable
carriers
vectorize
therapeutic
material
inside
target
cells.
Nanogels
are
highly
hydrated
nano-size
crosslinked
polymeric
networks
that
have
been
used
in
many
biomedical
applications,
drug
delivery
tissue
engineering
diagnostics.
Due
their
easy
production,
tunability,
swelling
properties
they
called
attention
as
promising
vectors
for
delivery.
In
this
review,
nanogels
discussed
acid
aiming
enlarge
therapy's
window.
Recent
works
highlighting
optimization
inherent
transfection
efficiency
biocompatibility
reviewed
here.
The
importance
monomer
choice,
along
with
internal
structure,
surface
decoration,
responsive
features
outlined
different
modalities.
possible
sources
toxicological
endpoints
analyzed,
strategies
limit
them
compared.
Finally,
perspectives
identify
remining
challenges
before
translation
market
agents.
Peptide Science,
Journal Year:
2023,
Volume and Issue:
116(2)
Published: July 9, 2023
Abstract
Bicyclic
peptides
have
emerged
as
one
of
the
driving
forces
within
constrained
peptide
family.
Due
to
their
unique
pharmaceutical
attributes,
bicycles
garnered
a
reputation
promising
therapeutics
future.
Combining
advantages
small
molecule
and
biologics,
target
binding
qualities
this
highly
class
molecules
been
likened
those
antibodies.
Over
past
5
years,
field
bicyclic
has
advanced
significantly.
New
secondary
metabolites
discovered
in
aquatic
organisms
and,
importantly,
diverse
assortment
synthesized
de
novo
,
exploring
new
chemical
scaffolds.
Biocompatible
synthetic
strategies
that
are
suitable
for
large
library
screening
platforms
helped
progress
into
clinical
trials.
This
review
aims
provide
comprehensive
overview
most
recent
field.
Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 16, 2024
Abstract
Polyhedral
oligomeric
silsesquioxane
(POSS)
is
a
3D,
cage‐like
nanoparticle
with
an
inorganic
Si–O–Si
core
and
eight
tunable
corner
functional
groups.
Its
well‐defined
structure
grants
it
distinctive
physical,
chemical,
biological
properties
has
been
widely
used
for
preparing
high‐performance
materials.
Recently,
click
chemistry
enabled
the
synthesis
of
various
POSS‐based
materials
diverse
biomedical
applications.
This
article
reviews
recent
applications
in
field,
including
cancer
treatment,
tissue
engineering,
antibacterial
use,
imaging.
Representative
examples
are
discussed
detail.
Among
applications,
treatment
engineering
most
important.
Finally,
this
review
presents
current
limitations
provides
guidance
future
research.
Organic Letters,
Journal Year:
2023,
Volume and Issue:
25(15), P. 2739 - 2744
Published: April 12, 2023
Both
azido
(N3)
and
trifluoromethyl
(CF3)
groups
are
key
moieties
of
numerous
valuable
molecules
that
extensively
applied
in
drug
discovery,
chemical
biology,
synthetic
chemistry.
However,
the
asymmetric
construction
chiral
quaternary
stereocenters
bearing
both
N3
CF3
is
still
unexplored.
Herein,
we
report
a
kind
bench-stable
easily
adjustable
benziodazolone-based
azidating
reagents.
These
reagents
were
used
to
achieve
an
enantioselective
copper-catalyzed
azidation
N-unprotected
3-trifluoromethylated
oxindoles
provide
diverse
enantioenriched
3-N3-3-CF3
oxindoles.
Molecules,
Journal Year:
2023,
Volume and Issue:
28(12), P. 4655 - 4655
Published: June 8, 2023
As
part
of
the
valorization
agricultural
waste
into
bioactive
compounds,
a
series
structurally
novel
oleanolic
acid
((3β-hydroxyolean-12-en-28-oic
acid,
OA-1)-phtalimidines
(isoindolinones)
conjugates
18a-u
bearing
1,2,3-triazole
moieties
were
designed
and
synthesized
by
treating
an
azide
4
previously
prepared
from
OA-1
isolated
olive
pomace
(Olea
europaea
L.)
with
wide
range
propargylated
phtalimidines
using
Cu(I)-catalyzed
click
chemistry
approach.
its
newly
analogues,
18a-u,
screened
in
vitro
for
their
antibacterial
activity
against
two
Gram-positive
bacteria,
Staphylococcus
aureus
Listeria
monocytogenes,
Gram-negative
Salmonella
thyphimurium
Pseudomonas
aeruginosa.
Attractive
results
obtained,
notably
L.
monocytogenes.
Compounds
18d,
18g,
18h
exhibited
highest
when
compared
other
compounds
tested
pathogenic
bacterial
strains.
A
molecular
docking
study
was
performed
to
explore
binding
mode
most
active
derivatives
site
ABC
substrate-binding
protein
Lmo0181
Results
showed
importance
both
hydrogen
bonding
hydrophobic
interactions
target
are
favor
experimental
data.
