Spatial metabolomics method to reveal the differences in chemical composition of raw and honey‐fried Stemona tuberosa Lour. by using UPLC‐Orbitrap Fusion MS and desorption electrospray ionization mass spectrometry imaging DOI

Haixuan Xiong,

Shuding Sun, Weiwei Zhang

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: July 28, 2024

Abstract Introduction Stemona tuberosa Lour. (ST) is a significant traditional Chinese medicine (TCM) renowned for its antitussive and insecticidal properties. ST commonly subjected to processing in clinical practice before being utilized as medicinal substance. Currently, the customary technique honey‐fried. Nevertheless, specific variations chemical constituents of after honey‐fried remain unclear. Objective This work aimed analyze study distribution differential markers roots. Methods UPLC‐Orbitrap Fusion MS combined with molecular network analysis was used metabolome (HST) screen metabolites by multivariate statistical analysis. Spatial metabolomics applied desorption electrospray ionization mass spectrometry imaging (DESI‐MSI). Results The HST exhibited notable disparities, 56 61 found from each, respectively. After processing, types alkaloids decreased, 12 were screened common compounds. component epibisdehydro‐tuberostemonine J, neostenine, tuberostemonine, croomine, neotuberostemonine, so forth. MSI visualized spatial metabolites. Conclusions Our research provided rapid effective visualization method identification ST. Compared method, this offered more convincing data supporting mechanism investigations macroscopic perspective.

Language: Английский

Spatial multiplexing and omics DOI
Julienne L. Carstens, Santhoshi Krishnan, Arvind Rao

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: Aug. 1, 2024

Language: Английский

Citations

8
Advances in imaging mass spectrometry for biomedical and clinical research DOI
Katerina Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 169, P. 117344 - 117344

Published: Oct. 14, 2023

Language: Английский

Citations

15
Mass spectrometry imaging in environmental monitoring: From a scarce existing past to a promising future DOI
Ana María Herruzo-Ruiz, Ángela Peralbo‐Molina, Cristina María López

et al.

Trends in Environmental Analytical Chemistry, Journal Year: 2024, Volume and Issue: 42, P. e00228 - e00228

Published: Feb. 3, 2024

Language: Английский

Citations

4
Mass spectrometry imaging in plants, microbes, and food: a review DOI Creative Commons

Mudita Vats,

Berta Cillero‐Pastor, Eva Cuypers

et al.

The Analyst, Journal Year: 2024, Volume and Issue: 149(18), P. 4553 - 4582

Published: Jan. 1, 2024

Plant health, which affects the nutritional quality and safety of derivative food products, is influenced by symbiotic interactions with microorganisms. These influence local molecular profile at tissue level. Therefore, studying distribution molecules within plants, microbes, plant-based crucial to assess plant ensure agricultural products that become part our supply, plan management practices. Within this framework, samples can be visualized mass spectrometry imaging (MSI). This review describes key MSI methodologies, highlighting role they play in unraveling localization metabolites, lipids, proteins, pigments, elemental components across products. Furthermore, investigations involve multimodal approaches combining other techniques are described. The advantages limitations different their applicability diverse agro-food studies described enable informed choices for tailored analyses. For example, some technologies meticulous sample preparation while others compromise spatial resolution gain throughput. Key parameters such as sensitivity, ionization bias fragmentation, reference database compound class specificity discussed review. With ongoing refinements instrumentation, data analysis, integration complementary techniques, deepens insight into biology ecosystem. turn empowers quest sustainable productive

Language: Английский

Citations

4
European Society of Toxicologic Pathology—Pathology 2.0 Mass Spectrometry Imaging Special Interest Group: Mass Spectrometry Imaging in Diagnostic and Toxicologic Pathology for Label-Free Detection of Molecules—From Basics to Practical Applications DOI
Enrico Vezzali, Michael W. Becker, F. Romero-Palomo

et al.

Toxicologic Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Mass Spectrometry Imaging (MSI) is a powerful tool to understand molecular pathophysiology and therapeutic toxicity mechanisms, as well for patient stratification precision medicine. MSI, label-free technique offering detailed spatial information on large number of molecules in different tissues, encompasses various techniques including Matrix-Assisted Laser Desorption Ionization (MALDI), Electrospray (DESI), Secondary Ion (SIMS) that can be applied diagnostic toxicologic pathology. Given the utmost importance high-quality samples, pathologists play pivotal role providing comprehensive pathobiology histopathology knowledge, tissue sampling, orientation, morphology, endogenous biomarkers, pathogenesis, which are crucial correct interpretation targeted experiments. This article introduces MSI its fundamentals, reports case examples, determining best suited technology address research questions. High-level principles characteristics most used modalities metabolomics, lipidomics proteomics, sensitivity specific requirements sample procurement preparation discussed. applications projects focused drug metabolism, nonclinical safety assessment, pharmacokinetics/pharmacodynamics pathology cases from clinical settings showcased.

Language: Английский

Citations

0
Spatial proteomics: unveiling the multidimensional landscape of protein localization in human diseases DOI Creative Commons
Mengyao Wu, Huihui Tao,

Tiantian Xu

et al.

Proteome Science, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 20, 2024

Language: Английский

Citations

3
Advances in Chromatographic and Mass Spectrometric Techniques for Analyzing Reducing Monosaccharides and Their Phosphates in Biological Samples DOI
Jiaqi Chen,

Yifeng Lou,

Yuwei Liu

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 23

Published: June 10, 2024

Reducing monosaccharides and their phosphates are critical metabolites in the central carbon metabolism pathway of living organisms. Variations content can indicate abnormalities metabolic pathways onset certain diseases, necessitating analysis detection. exhibit significant variations within biological samples present many isomers, which makes accurate quantification reducing a challenging task. Various analytical methods such as spectroscopy, fluorescence detection, colorimetry, nuclear magnetic resonance sensor-based techniques, chromatography, mass spectrometry employed to detect phosphates. In comparison, chromatography highly favored for ability simultaneously analyze multiple components high sensitivity selectivity. This review thoroughly evaluates current chromatographic spectrometric used detecting from 2013 2023, highlighting efficacy advancements these technologies.

Language: Английский

Citations

2
Desorption Electrospray Ionization-Mass Spectrometry Imaging-Based Spatial Metabolomics for Visualizing and Comparing Ginsenosides and Lipids among Multiple Parts and Positions of the Panax ginseng Root DOI
Hongda Wang, Li‐Li Hong, Feifei Yang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Ginsenosides and lipids are both bioactive ingredients for ginseng. Targeting these two categories of components, this study was designed to develop desorption electrospray ionization-mass spectrometry imaging approaches spatial metabolomics strategies, achieving the visualization differentiation among different parts Panax ginseng root (e.g., rhizome, main root, lateral fibrous adventitious root). Potential chemical markers were identified by searching an in-house ginsenoside library online Lipid Maps database, together with high-resolution MS2 data analysis. Six ginsenosides 11 diagnostic differentiate five P. root. Additionally, three 20 as differential six positions High-abundance malonyl- oleanolic acid-ginsenosides observed in rhizome. These results assist understanding accumulation molecules all through ginseng, which can benefit its quality control rational use.

Language: Английский

Citations

2
Mass spectrometry imaging reveals flavor distribution in edible mushrooms DOI Creative Commons

Mudita Vats,

Berta Cillero‐Pastor,

Bryn Flinders

et al.

Journal of Food Science and Technology, Journal Year: 2023, Volume and Issue: 61(5), P. 888 - 896

Published: Nov. 13, 2023

Abstract The spatial distribution of molecules and compounds responsible for the flavor profile edible button mushrooms ( Agaricus bisporous ) has never been determined. food industry is interested in knowing localization these compounds. Such knowledge would enable extraction from a particular regions mushroom, which safer consumption compared to alternatives such as synthetic flavoring agents. present study utilizes matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI), determine mushroom. As MALDI-MSI requires very thin sections, sample preparation protocol was optimized sectioning fresh frozen at 35 µm thickness considered best method evaluate Further, effect heat on investigated by heating whole 140 ℃ prior sectioning. Heating reduced water content mushroom thus enabled generation even-thinner 17 thick sections. measurements performed underivatized on-tissue derivatized heat-treated sections elucidated several flavor-related

Language: Английский

Citations

6
Accurate Clinical Detection of Vitamin D by Mass Spectrometry: A Review DOI

Zhilong Xu,

Kai Yu, Meng Zhang

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Feb. 20, 2024

Vitamin D deficiency is thought to be associated with a wide range of diseases, including diabetes, cancer, depression, neurodegenerative and cardiovascular cerebrovascular diseases. This vitamin global epidemic affecting both developing developed countries therefore qualitative quantitative analysis in clinical context essential. Mass spectrometry has played an increasingly important role the because its accuracy, sensitivity, specificity, ability detect multiple substances at same time. Despite their many advantages, mass spectrometry-based methods are not without analytical challenges. Front-end back-end challenges such as protein precipitation, analyte extraction, derivatization, spectrometer functionality, must carefully considered provide accurate robust through well-designed approach continuous control by internal external quality control. Therefore, aim this review comprehensive overview development for analysis, emphasis on status markers, deleterious effects biological matrices, derivatization reactions, ionization sources, contribution epimers, standardization assays between laboratories.

Language: Английский

Citations

1