Analysis of the Associations Between the Human Fecal Microbiome and Bone Density, Structure, and Strength: The Osteoporotic Fractures in Men (MrOS) Cohort DOI
Eric Orwoll,

Neeta Parimi,

Jack Wiedrick

et al.

Journal of Bone and Mineral Research, Journal Year: 2020, Volume and Issue: 37(4), P. 597 - 607

Published: Dec. 1, 2020

ABSTRACT In preclinical models, the composition and function of gut microbiota have been linked to bone growth homeostasis, but there are few available data from studies human populations. a hypothesis-generating experiment in large cohort community-dwelling older men (n = 831; age range, 78–98 years), we explored associations between fecal microbial profiles density, microarchitecture, strength measured with total hip dual-energy X-ray absorptiometry (DXA) high-resolution peripheral quantitative computed tomography (HRpQCT) (distal radius, distal diaphyseal tibia). Fecal samples were collected 16S rRNA gene V4 hypervariable region sequenced. Sequences bioinformatically processed through DADA2 pipeline then taxonomically assigned using SILVA. Generalized linear models as implemented microbiome multivariable association (MaAsLin 2) used test for skeletal measures specific genera. The abundances four bacterial genera weakly associated structure, or (false discovery rate [FDR] ≤ 0.05), directions generally consistent across multiple measures, supporting role on homeostasis. However, effect sizes small (log2 fold change < ±0.35), limiting power confidently identify these even high resolution imaging phenotypes, assessed resulting implications design future cohort-based studies. As analogous examples genomewide studies, find that larger will likely be needed health relying sequencing. Our findings bolster view is clinically important health, while also indicating challenges © 2022 American Society Bone Mineral Research (ASBMR).

Language: Английский

The microbiota-gut-bone axis and bone health DOI
Ye Tu, Ran Yang, Xin Xu

et al.

Journal of Leukocyte Biology, Journal Year: 2021, Volume and Issue: 110(3), P. 525 - 537

Published: April 22, 2021

The gastrointestinal tract is colonized by trillions of microorganisms, consisting bacteria, fungi, and viruses, known as the "second gene pool" human body. In recent years, microbiota-gut-bone axis has attracted increasing attention in field skeletal health/disorders. involvement gut microbial dysbiosis multiple bone disorders been recognized. microbiota regulates homeostasis through its effects on host metabolism, immune function, hormonal secretion. Owing to essential role homeostasis, novel microbiota-targeting therapeutics, such probiotics prebiotics, have proven effective preventing loss. However, more well-controlled clinical trials are still needed evaluate long-term efficacy safety these ecologic modulators treatment disorders.

Language: Английский

Citations

94
Are Probiotics the New Calcium and Vitamin D for Bone Health? DOI
René Rizzoli, Emmanuel Biver

Current Osteoporosis Reports, Journal Year: 2020, Volume and Issue: 18(3), P. 273 - 284

Published: April 13, 2020

Language: Английский

Citations

85
Nutritional influence on bone: role of gut microbiota DOI
René Rizzoli

Aging Clinical and Experimental Research, Journal Year: 2019, Volume and Issue: 31(6), P. 743 - 751

Published: Feb. 1, 2019

Language: Английский

Citations

66
Maternal fecal microbiome predicts gestational age, birth weight and neonatal growth in rural Zimbabwe. DOI Creative Commons
Ethan Gough,

Thaddeus J. Edens,

Hyun Min Geum

et al.

EBioMedicine, Journal Year: 2021, Volume and Issue: 68, P. 103421 - 103421

Published: June 1, 2021

BackgroundPreterm birth and low weight (LBW) affect one in ten seven livebirths, respectively, primarily low-income middle-income countries (LMIC) are major predictors of poor child health outcomes. However, both have been recalcitrant to public intervention. The maternal intestinal microbiome may undergo substantial changes during pregnancy influence fetal neonatal LMIC populations.MethodsWithin a subgroup 207 mothers infants enrolled the SHINE trial rural Zimbabwe, we performed shotgun metagenomics on 351 fecal specimens provided at 1-month post-partum investigate relationship between gut infant gestational age, weight, length-, weight-for-age z-scores using extreme gradient boosting machines.FindingsPregnancy taxa metabolic functions predicted WAZ 1 month more accurately than age LAZ. Blastoscystis sp, Brachyspira sp Treponeme carriage were high compared Western populations. Resistant starch-degraders important Microbiome capacity for environmental sensing, vitamin B metabolism, signalling increased growth; while involved biofilm formation response nutrient starvation reduced growth.InterpretationThe Zimbabwe is characterized by resistant be an target improve weight.FundingBill Melinda Gates Foundation, UK Department International Development, Wellcome Trust, Swiss Agency Development Cooperation, US National Institutes Health, UNICEF.

Language: Английский

Citations

52
Hen raising helps chicks establish gut microbiota in their early life and improve microbiota stability after H9N2 challenge DOI Creative Commons
Xiaobing Li, Ran Bi, Kangpeng Xiao

et al.

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Jan. 24, 2022

Abstract Background Early gut microbial colonization is important for postnatal growth and immune development of the chicken. However, at present, commercial chickens are hatched raised without adult hens, thus cut off from microbiota transfer between hens chicks. In this study, we compared composition hen-reared separately reared chicks, its impact on resistance to H9N2 avian influenza virus, with motive investigating cutoff in transfer. Results We used 16SrRNA sequencing method assess chicks represented by three groups one group. found that diversity microbes was more abundant than group, both phylum genus levels. Our findings highlight importance early parental care influencing establishment life SourceTracker analysis showed feather cloaca main sources After exposure, viral infection lasted longer titers their oropharyngeal swabs being higher day 5 post-infection. Interestingly, our results revealed stable after comparison Conclusions Microbiota promotes a balanced diverse improves stability challenge. These advance understanding protective role should be instrumental improving chick rearing poultry industry.

Language: Английский

Citations

33
Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets DOI
Shuai Chen, Bie Tan, Yaoyao Xia

et al.

Food & Function, Journal Year: 2018, Volume and Issue: 10(1), P. 366 - 378

Published: Dec. 14, 2018

This study aims to investigate the effects of dietary gamma-aminobutyric acid (GABA) supplementation on growth performance, intestinal immunity, GABAergic system, amino profiles and gut microflora weaned piglets. Totally sixteen healthy piglets were randomly assigned into two groups be fed with basal diet (Con group) or GABA (20 mg kg-1) supplementation. Body weights feed intakes monitored weekly. Piglets sacrificed after 3 weeks collect blood, ileum, ileal mucosa luminal content. Immune-associated factors, profiles, microbiota in ileum serum explored. The results showed that improved performance modulated immunity inhibiting gene expressions IL-22, proinflammatory cytokines (IL-1 IL-18), Muc1, but promoted anti-inflammatory (IFN-γ, IL-4, IL-10), TLR6 MyD88. regulated a few components increased levels most acids reduced profiles. population diversity microbiota, such as abundances dominant microbial populations, community richness, microbiota. In conclusion, functions, including can helpful for understanding functions intestine.

Language: Английский

Citations

53
Hydrolytic Profile of the Culturable Gut Bacterial Community Associated With Hermetia illucens DOI Creative Commons
Matteo Callegari, C. Jucker, Marco Fusi

et al.

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Aug. 12, 2020

Larvae of the black soldier fly (BSF) Hermetia illucens (L.) convert organic waste into high valuable insect biomass that can be used as alternative protein source for animal nutrition or feedstock biodiesel production. Since biology and physiology are influenced by gut microbiome, knowledge about functional role BSF-associated microorganisms could exploited to enhance performance growth. Although an increasing number culture-independent studies unveiling microbiota structure composition BSF microbiota, a gap remains on experimental validation contribution growth development. We aimed at assessing if gut-associated bacteria potentially involved in breakdown diet components able improve host nutrition. A total 193 bacterial strains were obtained from guts larvae reared nutritious using selective enrichment media. Most isolates typically found gut, with major representatives belonging Gammaproteobacteria Bacilli classes. The hydrolytic profile collection was assessed compounds present diet. Finally, we tested hypothesis addition nutritionally poor two Bacillus licheniformis HI169 Stenotrophomonas maltophilia HI121, selected their complementary metabolic activities, B. positively larval final weight rate when compared control. Conversely, S. HI121 did not result enhancement terms pupal length comparison control, whereas combination affected length. In conclusion, isolated potential positive properties showed may growth, suggesting importance evaluate effect administration performance.

Language: Английский

Citations

49
Parental exposure to sulfamethazine and nanoplastics alters the gut microbial communities in the offspring of marine madaka (Oryzias melastigma) DOI
Shuiqing He, Dan Li, Feipeng Wang

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 423, P. 127003 - 127003

Published: Aug. 21, 2021

Language: Английский

Citations

34
Animal development in the microbial world: Re-thinking the conceptual framework DOI
Thomas C. G. Bosch, Margaret McFall‐Ngai

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2021, Volume and Issue: unknown, P. 399 - 427

Published: Jan. 1, 2021

Language: Английский

Citations

33
The Function and the Affecting Factors of the Zebrafish Gut Microbiota DOI Creative Commons
Pingping Li, Jinhua Zhang, Xiaoyi Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 2, 2022

Gut microbiota has become a topical issue in unraveling the research mechanisms underlying disease onset and progression. As an important potential “organ,” gut plays role regulating intestinal epithelial cell differentiation, proliferation, metabolic function immune response, angiogenesis host growth. More recently, zebrafish models have been used to study interactions between hosts. It several advantages, such as short reproductive cycle, low rearing cost, transparent larvae, high genomic similarity humans, easy construction of germ-free (GF) transgenic zebrafish. In our review, we reviewed large amount data focusing on close relationship health. Moreover, outlined functions function, response. More, summarized major factors that can influence composition, abundance, diversity microbiota, which will help us understand significance biological provide options for maintaining balance

Language: Английский

Citations

27