Analysis of the Associations Between the Human Fecal Microbiome and Bone Density, Structure, and Strength: The Osteoporotic Fractures in Men (MrOS) Cohort DOI
Eric Orwoll,

Neeta Parimi,

Jack Wiedrick

et al.

Journal of Bone and Mineral Research, Journal Year: 2020, Volume and Issue: 37(4), P. 597 - 607

Published: Dec. 1, 2020

ABSTRACT In preclinical models, the composition and function of gut microbiota have been linked to bone growth homeostasis, but there are few available data from studies human populations. a hypothesis-generating experiment in large cohort community-dwelling older men (n = 831; age range, 78–98 years), we explored associations between fecal microbial profiles density, microarchitecture, strength measured with total hip dual-energy X-ray absorptiometry (DXA) high-resolution peripheral quantitative computed tomography (HRpQCT) (distal radius, distal diaphyseal tibia). Fecal samples were collected 16S rRNA gene V4 hypervariable region sequenced. Sequences bioinformatically processed through DADA2 pipeline then taxonomically assigned using SILVA. Generalized linear models as implemented microbiome multivariable association (MaAsLin 2) used test for skeletal measures specific genera. The abundances four bacterial genera weakly associated structure, or (false discovery rate [FDR] ≤ 0.05), directions generally consistent across multiple measures, supporting role on homeostasis. However, effect sizes small (log2 fold change < ±0.35), limiting power confidently identify these even high resolution imaging phenotypes, assessed resulting implications design future cohort-based studies. As analogous examples genomewide studies, find that larger will likely be needed health relying sequencing. Our findings bolster view is clinically important health, while also indicating challenges © 2022 American Society Bone Mineral Research (ASBMR).

Language: Английский

Gut microbiota development in the growing dog: A dynamic process influenced by maternal, environmental and host factors DOI Creative Commons

Quentin Garrigues,

Emmanuelle Apper, Sylvie Chastant‐Maillard

et al.

Frontiers in Veterinary Science, Journal Year: 2022, Volume and Issue: 9

Published: Sept. 2, 2022

Microorganisms of the gastrointestinal tract play a crucial role in health, metabolism and development their host by modulating vital functions such as digestion, production key metabolites or stimulation immune system. This review aims to provide an overview on current knowledge factors shaping gut microbiota young dogs. The composition is modulated many intrinsic (i.e., age, physiology, pathology) extrinsic nutrition, environment, medication) which can cause both beneficial harmful effects depending nature changes. quickly evolving during early dog, some bacteria, mostly anaerobic, progressively colonize before puppy reaches adulthood. Those bacterial communities are paramount importance for with disturbance potentially leading altered metabolic states acute diarrhea inflammatory bowel disease. While studies focused children, there still lack concerning puppies. Understanding this evolution becoming aspect improve dogs' short long-term health wellbeing.

Language: Английский

Citations

26
Geraniol-a potential alternative to antibiotics for bovine mastitis treatment without disturbing the host microbial community or causing drug residues and resistance DOI Creative Commons
Wei Guo,

Min Qiu,

Zhonghui Pu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Feb. 16, 2023

Mastitis is one of the most prevalent diseases dairy cows. Currently, mastitis treatment in cows mainly based on antibiotics. However, use antibiotics causes adverse effects, including drug resistance, residues, host-microbiome destruction, and environmental pollution. The present study sought to investigate potentiality geraniol as an alternative for bovine Additionally, effectiveness treatment, improvement inflammatory factors, influence microbiome, presence resistance induction were compared analyzed comprehensively.Geraniol showed equivalent therapeutic rate mouse infection model with mastitis. Moreover, significantly inhibited pathogenic bacteria restored microbial community while increasing abundance probiotics milk. Notably, did not destroy gut communities mice, whereas reduced diversity destroyed structure. no residue was detected milk four days after discontinuation, but, antibiotic residues at 7th day withdrawal. In vitro experiments revealed that induce Escherichia coli strain ATCC25922 Staphylococcus aureus ATCC25923 150 generations culturing, induced 10 generations. These results suggest has antibacterial anti-inflammatory effects similar without affecting host-microbial structure or causing resistance. Therefore, can be a potential substitute treat other infectious widely used industry.

Language: Английский

Citations

11
The diversity, composition, network characteristics and community assembly of intestinal microbiome in sea cucumber reflect the differences in habitats and aquaculture practices DOI
Cui Liang, Bing Wang, Kai Luo

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 376, P. 124487 - 124487

Published: Feb. 10, 2025

Language: Английский

Citations

0
The effects of fermented Astragalus polysaccharides on the growth performance, antioxidant capacity and intestinal health of broilers DOI Creative Commons

Zhenkun Liu,

Huaidan Zhang,

Xianxin Chen

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 25, 2025

This study aimed to investigate the effects of fermented Astragalus polysaccharides(FAP) on growth performance, antioxidant capacity and intestinal health broilers. A total 1,080 Cyan-shank Partridge chickens were divided into 4 groups, with 6 replicates per group 45 replicate. Add 0% (T1), 0.2% (T5), 0.4% (T6) 0.6% (T7) FAP basal diet, respectively. The trial lasted for 42 days. results indicated that, compared T1 group, FW ADG broilers in each treatment significantly increased (p < 0.05). slaughter rates T6 T7 groups higher meanwhile, carcass yields T5, T6, notably enhanced Compared activities CAT, GSH-Px content T-AOC 0.05), while MDA was decreased All exhibited VH VH/CD duodenum relative mRNA expression levels ZO-1 Claudin jejunal mucosa all up-regulated, expressions IL-1β, IL-6, TNF-α, IFN-γ down-regulated 16S rDNA sequencing analysis revealed that at phylum level, abundance Verrucomicrobiota Cyanobacteria, Nitrospirota, Elusimicrobiota, Acidobacteriota unique Cyanobacteria Elusimicrobiota T5 group. At genus Desulfovibrio reduced Additionally, polysaccharides Bacteroidota, Campilobacterota, Deferribacterota, Firmicutes, Fusobacteriota, Proteobacteria, Spirochaetota LEfSe found Clostridia_vadinBB60_group Comamonas identified as potential biomarkers. Overall, feeding can enhance characteristics, broiler by modulating gut microbiota strengthening barrier function.

Language: Английский

Citations

0
Duan-Nai-An, A Yeast Probiotic, Improves Intestinal Mucosa Integrity and Immune Function in Weaned Piglets DOI Creative Commons
Yingpai Zhaxi,

Xiaoqin Meng,

Wenhui Wang

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: March 12, 2020

Post-weaning diarrhea commonly occurs in piglets and results significant economic loss to swine producers. Non-antibiotic measures for managing post-weaning are critically needed. Duan-Nai-An, a probiotic produced from the yeast fermentation of egg whites, was previously shown optimize intestinal flora reduce incidence clinical weaning piglets. To study effects Duan-Nai-An on mucosal integrity immunity pig intestine, we examined microstructure ultrastructure intestines weaned pigs with or without as feed supplement. The Duan-Nai-An-fed group developed intact columnar epithelia covered by tightly packed microvilli apical surface. However, control (no supplement) showed villous atrophy thinning, microvillus slough, severe cases, damage exposure underlying lamina propria. Moreover, apparent plasmocyte hyperplasia, increased lymphoid nodule numbers, well-developed Peyer's Patchs, germinal centers. tissues were far less developed, showing lymph node atrophy, lymphocyte reduction, degeneration, necrosis. These indicate that improves development structures promotes health

Language: Английский

Citations

30
Impact of Parenteral Ceftiofur on Developmental Dynamics of Early Life Fecal Microbiota and Antibiotic Resistome in Neonatal Lambs DOI Creative Commons

Mohamed Donia,

Nasr‐Eldin M. Aref, Mohamed Zeineldin

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 434 - 434

Published: April 25, 2025

Background: Early gut microbiome development is critical for neonatal health, and its dysbiosis may impact long-term animal productivity. This study examined the effects of parenteral Ceftiofur Crystalline Free Acid (CCFA) on composition diversity lamb fecal microbiome. The emergence antimicrobial resistance genes associated with CCFA exposure was also investigated. Results: There were distinct microbial populations in CCFA-treated lambs compared to control group at each time point, a highly significant decrease alpha beta diversity. treatment showed reduction several key taxa during nursing, but these differences diminished by day 56. Unlike group, had core microbes potentially carrying multiple antibiotic genes, including those beta-lactam, fosfomycin, methicillin, multidrug resistance. Methods: Twenty-four healthy randomly assigned (n = 12) groups. Fecal samples collected days 0, 7, 14, 28, Genomic DNA extracted sequenced using Illumina MiSeq platform. Microbial analyzed MG-RAST pipeline RefSeq database. Conclusions: Despite temporary reductions bacterial early sheep demonstrated resilience repopulating after disruption. While this highlights microbiota stability short-course exposure, transient disturbance underscores potential risks health. Importantly, persistent poses environmental dissemination risks, emphasizing need cautious use livestock mitigate ecological impacts.

Language: Английский

Citations

0
Faecal microbiota and functional capacity associated with weaning weight in meat rabbits DOI Creative Commons
Shaoming Fang, Xuan Chen, Liwen Zhou

et al.

Microbial Biotechnology, Journal Year: 2019, Volume and Issue: 12(6), P. 1441 - 1452

Published: Oct. 1, 2019

Weaning weight is an important economic trait in the meat rabbit industry. Evidence has linked gut microbiota to health and production performance rabbits. However, effect of on weaning remains unclear. In this study, we performed 16S rRNA gene sequencing analysis 135 faecal samples from commercial Ira We detected 50 OTUs significantly associated with weight. that showed positive associations were mostly members family Ruminococcaceae which are degrading dietary fibres producing butyrate. On contrary, annotated genera Blautia, Lachnoclostridium Butyricicoccus correlated fat deposition negatively Predicted functional capacity revealed 91 KOs 26 KEGG pathways exhibited potential correlations found involved metabolism amino acids, butanoate, energy monosaccharides affected Additionally, cross-validation indicated 16.16% variation was explained by microbiome. Our findings provide information improve rabbits modulating their

Language: Английский

Citations

27
Characteristics of Gut Microbiome and Its Metabolites, Short-Chain Fatty Acids, in Children With Idiopathic Short Stature DOI Creative Commons
Lin Li, Lifen Chen,

Yuanyan Yang

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: June 10, 2022

Background The gut microbiome is important for host nutrition and metabolism. Whether the under normal diet regulate human height remains to be addressed. Our study explored possible relationship between microbiota, its metabolic products pathogenesis of idiopathic short stature disease (ISS) by comparing microbiota children with ISS height, also short-chain fatty acids (SCFAs) produced microbiota. Methods subjects this were 32 prepubescent aged 4-8 years. fecal microbial structure was analyzed 16S rRNA high-throughput sequencing technology. concentrations SCFAs in feces determined gas chromatography-mass spectrometry. Results richness group decreased, composition significantly different control group. relative abundance nine species including family Ruminococcaceae genera Faecalibacterium Eubacterium , lower than that (P&lt;0.05). 10 species, such as those belonging genus Parabacteroides Clostridium higher concentration total butyrate correlation analysis among clinical indicators, showed positively correlated standard deviation score height. Furthermore, serum insulin-like growth factor 1 (IGF-1)-SDS. Disease prediction model constructed based on bacteria who differed healthy exhibited high diagnostic value (AUC: 0.88). Conclusions change metabolite levels may related pathogenesis. Strains increased or decreased specificity could used biomarkers diagnose ISS.

Language: Английский

Citations

16
The nutritional and functional properties of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol-rich oil: promoting early-life growth and intestinal health with alterations in the intestinal microbiota of Micropterus salmoides DOI

Meng Kang,

Konglong Feng,

Weijie Dai

et al.

Food & Function, Journal Year: 2023, Volume and Issue: 14(9), P. 4092 - 4105

Published: Jan. 1, 2023

1-Oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), a key structural lipid in the breast milk fat, plays critical role providing nutrients and energy for infants. OPL is more abundant Chinese fat might be better infants' growth. However, few studies have investigated effect of on growth intestinal health organism early life. OPL-rich oil with 45.77% was prepared by immobilized lipase-catalyzed synthesis purification. The effects nutritional properties regulation microbiota life were further vivo (Micropterus salmoides). Dietary significantly increased juvenile fish weight gain rate, protein content, muscular polyunsaturated fatty acids, which turn markedly altered muscle texture springiness cohesiveness. could also protect tissues increasing fold height, mucosal thickness, wall thickness. Furthermore, dietary regulated microbiota. Particularly, probiotics (Cetobacterium_sp014250685, Streptomyces_mutabilis, Saccharopolyspora_spinosa, Nocardiopsis_kunsanensis) reduced potential pathogens (Staphylococcus_nepalensis, Salmonella_enterica, Candidatus_berkiella). structured promoted improved composition due to its higher bioavailability relative tripalmitate (PPP). Moreover, growth, cholesterol metabolism, than mixed (MO), attributed palmitic acid content sn-2 position. Overall, structure triacylglycerols distribution acids affected health, effective improvement health.

Language: Английский

Citations

9
Structure–function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct cues supporting Drosophila juvenile growth DOI Creative Commons
Nikos Nikolopoulos, Renata Matos, Stéphanie Ravaud

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: April 12, 2023

Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by

Language: Английский

Citations

8